Planaria EST Database


DrC_01060

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01060
         (994 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P07602|SAP_HUMAN  Proactivator polypeptide precursor [Con...    45   2e-04
sp|P26779|SAP_BOVIN  Proactivator polypeptide precursor [Con...    43   0.002
sp|Q8XNE2|NADB_CLOPE  L-aspartate oxidase (LASPO) (Quinolina...    35   0.43 
sp|P30337|CHIN_RAT  N-chimaerin (NC) (N-chimerin) (Alpha chi...    32   2.1  
sp|Q6RZJ4|V27K_RABPU  27 kDa virion core protein                   32   2.1  
sp|P54757|EPHA5_RAT  Ephrin type-A receptor 5 precursor (Tyr...    32   2.1  
sp|P54756|EPHA5_HUMAN  Ephrin type-A receptor 5 precursor (T...    32   2.8  
sp|Q6L0Y7|TRUA_PICTO  tRNA pseudouridine synthase A (tRNA-ur...    32   3.6  
sp|Q91V57|CHIN_MOUSE  N-chimaerin (NC) (N-chimerin) (Alpha c...    31   4.8  
sp|P69037|VFP_NPVAC  FP protein (25 kDa protein) >gi|6880566...    31   4.8  
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
           A); Saposin B-Val; Saposin B (Sphingolipid activator
           protein 1) (SAP-1) (Cerebroside sulfate activator)
           (CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
           (Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
           activator) (Sphingolipid activator protein 2) (SAP-2);
           Saposin D (Protein C) (Component C)]
          Length = 524

 Score = 45.4 bits (106), Expect = 2e-04
 Identities = 39/175 (22%), Positives = 74/175 (42%)
 Frame = +3

Query: 333 SEKFCNRCENIPESKFLKWAKSVDIKMLTDAGYKVMCSELNTTESERCREIISHIGTVIL 512
           S+ +C  CE + +        +   K + DA +  MCS+L  + SE C+E++   G+ IL
Sbjct: 311 SDVYCEVCEFLVKEVTKLIDNNKTEKEILDA-FDKMCSKLPKSLSEECQEVVDTYGSSIL 369

Query: 513 HELENVLSVKNVCYKIYRCPMNLETTNEMHSFYYDTFDFTRQTSKRCEVCKAFSDVFSSG 692
             L   +S + VC  ++ C         +H        F       CEVCK         
Sbjct: 370 SILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGF-------CEVCKKLVGYLDRN 422

Query: 693 IDRDLFEEMVVDQFEKVYCNKLFPKKEHMCKKFLKNELPNILNKLRVSIHRDYLC 857
           ++++  ++ ++   EK  C+ L    +  C +F+    P ++  L   +   ++C
Sbjct: 423 LEKNSTKQEILAALEK-GCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVC 476
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
           A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
           (Cerebroside sulfate activator) (CSAct) (Dispersin)
           (Sulfatide/GM1 activator); Saposin C
           (Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
           activator) (Sphingolipid activator protein 2) (SAP-2);
           Saposin D (Protein C) (Component C)]
          Length = 525

 Score = 42.7 bits (99), Expect = 0.002
 Identities = 33/143 (23%), Positives = 63/143 (44%), Gaps = 3/143 (2%)
 Frame = +3

Query: 438 MCSELNTTESERCREIISHIGTVILHELENVLSVKNVCYKIYRCP---MNLETTNEMHSF 608
           +CS+L T+ +E+C+E++   G  IL  L +  S + VC  ++ C    +   T   M   
Sbjct: 346 VCSKLPTSLAEQCQEVVDTYGRSILSILLDEASPELVCSMLHLCSSRGLPAATVRVM--- 402

Query: 609 YYDTFDFTRQTSKRCEVCKAFSDVFSSGIDRDLFEEMVVDQFEKVYCNKLFPKKEHMCKK 788
                   R+    CEVCK         ++++  +E ++   EK  C+ L  +    C +
Sbjct: 403 -------PRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAALEK-GCSFLPDQYRKQCDQ 454

Query: 789 FLKNELPNILNKLRVSIHRDYLC 857
           F+    P ++  L   +   ++C
Sbjct: 455 FVTEYEPVLIEILVEVMDPSFVC 477
>sp|Q8XNE2|NADB_CLOPE L-aspartate oxidase (LASPO) (Quinolinate synthetase B)
          Length = 439

 Score = 34.7 bits (78), Expect = 0.43
 Identities = 15/44 (34%), Positives = 24/44 (54%)
 Frame = +3

Query: 693 IDRDLFEEMVVDQFEKVYCNKLFPKKEHMCKKFLKNELPNILNK 824
           + RD+  + + ++ +K   N +F     M K FL+N  PNI NK
Sbjct: 265 LPRDIVSKKIYEEMKKTNSNNVFLDVSFMEKSFLQNRFPNIYNK 308
>sp|P30337|CHIN_RAT N-chimaerin (NC) (N-chimerin) (Alpha chimerin) (A-chimaerin)
           (Rho-GTPase-activating protein 2)
          Length = 334

 Score = 32.3 bits (72), Expect = 2.1
 Identities = 19/58 (32%), Positives = 33/58 (56%)
 Frame = +3

Query: 375 KFLKWAKSVDIKMLTDAGYKVMCSELNTTESERCREIISHIGTVILHELENVLSVKNV 548
           KF++ AK VD     +  ++ + S L     E  R +++H+  V LHE EN++S +N+
Sbjct: 237 KFIESAKIVDPDEQLETLHEALRS-LPPAHCETLRYLMAHLKRVTLHEKENLMSAENL 293
>sp|Q6RZJ4|V27K_RABPU 27 kDa virion core protein
          Length = 237

 Score = 32.3 bits (72), Expect = 2.1
 Identities = 30/124 (24%), Positives = 53/124 (42%), Gaps = 6/124 (4%)
 Frame = +3

Query: 261 NHNNFYDYFFTD-EYHDFFERDQKPSEKFCNRCENIPESKFLKWAKSVDIKMLTDAGYKV 437
           NHN+F     T  + H FF       +K+ + C  +  S F    + ++++   DAG +V
Sbjct: 27  NHNDFIRSKLTKLKEHVFFSEYIVTPDKYGSLCVELNGSSFQHGGRYIEVEEFIDAGRQV 86

Query: 438 -MCSELNTTESERCREIISHIGTVILHELENVLSVKNVCYKIYRCPMNL----ETTNEMH 602
             CS  N    +   +I  H    +++++    + KN      + P +L      TN + 
Sbjct: 87  RWCSTSNHISEDIPEDI--HTDKFVIYDIYTFDAFKNKRLVFVQVPPSLGDDSYLTNPLL 144

Query: 603 SFYY 614
           S YY
Sbjct: 145 SPYY 148
>sp|P54757|EPHA5_RAT Ephrin type-A receptor 5 precursor (Tyrosine-protein kinase
           receptor EHK-1) (EPH homology kinase 1)
          Length = 1005

 Score = 32.3 bits (72), Expect = 2.1
 Identities = 19/73 (26%), Positives = 29/73 (39%), Gaps = 9/73 (12%)
 Frame = +3

Query: 306 DFFERDQKPSEKFCNRCENIPE---------SKFLKWAKSVDIKMLTDAGYKVMCSELNT 458
           D+F R+  P    C R  + P          S FL+W    D     D  Y ++C + N 
Sbjct: 343 DYFRRESDPPTMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGGKDVSYYILCKKCN- 401

Query: 459 TESERCREIISHI 497
           + +  C E   H+
Sbjct: 402 SHAGVCEECGGHV 414
>sp|P54756|EPHA5_HUMAN Ephrin type-A receptor 5 precursor (Tyrosine-protein kinase
           receptor EHK-1) (EPH homology kinase 1) (Receptor
           protein-tyrosine kinase HEK7)
          Length = 1037

 Score = 32.0 bits (71), Expect = 2.8
 Identities = 19/73 (26%), Positives = 28/73 (38%), Gaps = 9/73 (12%)
 Frame = +3

Query: 306 DFFERDQKPSEKFCNRCENIPE---------SKFLKWAKSVDIKMLTDAGYKVMCSELNT 458
           D+F R+  P    C R  + P          S FL+W    D     D  Y + C + N 
Sbjct: 341 DYFRRESDPPTMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCN- 399

Query: 459 TESERCREIISHI 497
           + +  C E   H+
Sbjct: 400 SHAGVCEECGGHV 412
>sp|Q6L0Y7|TRUA_PICTO tRNA pseudouridine synthase A (tRNA-uridine isomerase I) (tRNA
           pseudouridylate synthase I)
          Length = 251

 Score = 31.6 bits (70), Expect = 3.6
 Identities = 10/26 (38%), Positives = 15/26 (57%)
 Frame = +3

Query: 207 HQFFHEYFFNNQYTSPTFNHNNFYDY 284
           + FFH Y+ +N Y +P +N    Y Y
Sbjct: 87  NMFFHSYYLSNSYINPRYNSMKIYKY 112
>sp|Q91V57|CHIN_MOUSE N-chimaerin (NC) (N-chimerin) (Alpha chimerin) (A-chimaerin)
           (Rho-GTPase-activating protein 2)
          Length = 334

 Score = 31.2 bits (69), Expect = 4.8
 Identities = 18/58 (31%), Positives = 33/58 (56%)
 Frame = +3

Query: 375 KFLKWAKSVDIKMLTDAGYKVMCSELNTTESERCREIISHIGTVILHELENVLSVKNV 548
           KF++ AK +D     +  ++ + S L     E  R +++H+  V LHE EN++S +N+
Sbjct: 237 KFIESAKIMDPDEQLETLHEALRS-LPPAHCETLRYLMAHLKRVTLHEKENLMSAENL 293
>sp|P69037|VFP_NPVAC FP protein (25 kDa protein)
 sp|P69038|VFP_NPVGM FP protein (25 kDa protein)
          Length = 214

 Score = 31.2 bits (69), Expect = 4.8
 Identities = 13/46 (28%), Positives = 26/46 (56%)
 Frame = +1

Query: 88  MNIQHIIINIFMKHHIPKIICFHTNISNISTKIKQQIISHINFSMN 225
           +N +H+I + F K+HI   +C  T      TK +++ + + N ++N
Sbjct: 77  LNFKHVIESSFDKNHIVAKLCDATRAKEWQTKSRERRLKNFNLNIN 122
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109,883,005
Number of Sequences: 369166
Number of extensions: 2371267
Number of successful extensions: 7097
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6649
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7076
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10479017505
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01060

  1. Dr_sW_026_K17
  2. Dr_sW_022_K21