Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_K17
(867 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 43 0.001
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 40 0.006
sp|P30337|CHIN_RAT N-chimaerin (NC) (N-chimerin) (Alpha chi... 32 1.7
sp|Q6RZJ4|V27K_RABPU 27 kDa virion core protein 32 1.7
sp|P54757|EPHA5_RAT Ephrin type-A receptor 5 precursor (Tyr... 32 1.7
sp|P54756|EPHA5_HUMAN Ephrin type-A receptor 5 precursor (T... 32 2.3
sp|Q6L0Y7|TRUA_PICTO tRNA pseudouridine synthase A (tRNA-ur... 32 3.0
sp|Q91V57|CHIN_MOUSE N-chimaerin (NC) (N-chimerin) (Alpha c... 31 3.9
sp|P50436|GLYA_METTM Serine hydroxymethyltransferase (Serin... 31 3.9
sp|P69037|VFP_NPVAC FP protein (25 kDa protein) >gi|6880566... 31 3.9
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 43.1 bits (100), Expect = 0.001
Identities = 37/159 (23%), Positives = 67/159 (42%)
Frame = +3
Query: 333 SEKFCNRCENIPESKFLKWAKSVDIKMLTDAGYKVMCSELNTTESERCREIISHIGTVIL 512
S+ +C CE + + + K + DA + MCS+L + SE C+E++ G+ IL
Sbjct: 311 SDVYCEVCEFLVKEVTKLIDNNKTEKEILDA-FDKMCSKLPKSLSEECQEVVDTYGSSIL 369
Query: 513 HELENVLSVKNVCYKIYRCPMNLETTNEMHSFYYDTFDFTRQTSKRCEVCKAFSDVFSSG 692
L +S + VC ++ C +H F CEVCK
Sbjct: 370 SILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGF-------CEVCKKLVGYLDRN 422
Query: 693 IDRDLFEEMVVDQFEKVYCNKLFPKKEHMCKKFLKNELP 809
++++ ++ ++ EK C+ L + C +F+ P
Sbjct: 423 LEKNSTKQEILAALEK-GCSFLPDPYQKQCDQFVAEYEP 460
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 40.4 bits (93), Expect = 0.006
Identities = 31/127 (24%), Positives = 56/127 (44%), Gaps = 3/127 (2%)
Frame = +3
Query: 438 MCSELNTTESERCREIISHIGTVILHELENVLSVKNVCYKIYRCP---MNLETTNEMHSF 608
+CS+L T+ +E+C+E++ G IL L + S + VC ++ C + T M
Sbjct: 346 VCSKLPTSLAEQCQEVVDTYGRSILSILLDEASPELVCSMLHLCSSRGLPAATVRVM--- 402
Query: 609 YYDTFDFTRQTSKRCEVCKAFSDVFSSGIDRDLFEEMVVDQFEKVYCNKLFPKKEHMCKK 788
R+ CEVCK ++++ +E ++ EK C+ L + C +
Sbjct: 403 -------PRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAALEK-GCSFLPDQYRKQCDQ 454
Query: 789 FLKNELP 809
F+ P
Sbjct: 455 FVTEYEP 461
>sp|P30337|CHIN_RAT N-chimaerin (NC) (N-chimerin) (Alpha chimerin) (A-chimaerin)
(Rho-GTPase-activating protein 2)
Length = 334
Score = 32.3 bits (72), Expect = 1.7
Identities = 19/58 (32%), Positives = 33/58 (56%)
Frame = +3
Query: 375 KFLKWAKSVDIKMLTDAGYKVMCSELNTTESERCREIISHIGTVILHELENVLSVKNV 548
KF++ AK VD + ++ + S L E R +++H+ V LHE EN++S +N+
Sbjct: 237 KFIESAKIVDPDEQLETLHEALRS-LPPAHCETLRYLMAHLKRVTLHEKENLMSAENL 293
>sp|Q6RZJ4|V27K_RABPU 27 kDa virion core protein
Length = 237
Score = 32.3 bits (72), Expect = 1.7
Identities = 30/124 (24%), Positives = 53/124 (42%), Gaps = 6/124 (4%)
Frame = +3
Query: 261 NHNNFYDYFFTD-EYHDFFERDQKPSEKFCNRCENIPESKFLKWAKSVDIKMLTDAGYKV 437
NHN+F T + H FF +K+ + C + S F + ++++ DAG +V
Sbjct: 27 NHNDFIRSKLTKLKEHVFFSEYIVTPDKYGSLCVELNGSSFQHGGRYIEVEEFIDAGRQV 86
Query: 438 -MCSELNTTESERCREIISHIGTVILHELENVLSVKNVCYKIYRCPMNL----ETTNEMH 602
CS N + +I H +++++ + KN + P +L TN +
Sbjct: 87 RWCSTSNHISEDIPEDI--HTDKFVIYDIYTFDAFKNKRLVFVQVPPSLGDDSYLTNPLL 144
Query: 603 SFYY 614
S YY
Sbjct: 145 SPYY 148
>sp|P54757|EPHA5_RAT Ephrin type-A receptor 5 precursor (Tyrosine-protein kinase
receptor EHK-1) (EPH homology kinase 1)
Length = 1005
Score = 32.3 bits (72), Expect = 1.7
Identities = 19/73 (26%), Positives = 29/73 (39%), Gaps = 9/73 (12%)
Frame = +3
Query: 306 DFFERDQKPSEKFCNRCENIPE---------SKFLKWAKSVDIKMLTDAGYKVMCSELNT 458
D+F R+ P C R + P S FL+W D D Y ++C + N
Sbjct: 343 DYFRRESDPPTMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGGKDVSYYILCKKCN- 401
Query: 459 TESERCREIISHI 497
+ + C E H+
Sbjct: 402 SHAGVCEECGGHV 414
>sp|P54756|EPHA5_HUMAN Ephrin type-A receptor 5 precursor (Tyrosine-protein kinase
receptor EHK-1) (EPH homology kinase 1) (Receptor
protein-tyrosine kinase HEK7)
Length = 1037
Score = 32.0 bits (71), Expect = 2.3
Identities = 19/73 (26%), Positives = 28/73 (38%), Gaps = 9/73 (12%)
Frame = +3
Query: 306 DFFERDQKPSEKFCNRCENIPE---------SKFLKWAKSVDIKMLTDAGYKVMCSELNT 458
D+F R+ P C R + P S FL+W D D Y + C + N
Sbjct: 341 DYFRRESDPPTMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCN- 399
Query: 459 TESERCREIISHI 497
+ + C E H+
Sbjct: 400 SHAGVCEECGGHV 412
>sp|Q6L0Y7|TRUA_PICTO tRNA pseudouridine synthase A (tRNA-uridine isomerase I) (tRNA
pseudouridylate synthase I)
Length = 251
Score = 31.6 bits (70), Expect = 3.0
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +3
Query: 207 HQFFHEYFFNNQYTSPTFNHNNFYDY 284
+ FFH Y+ +N Y +P +N Y Y
Sbjct: 87 NMFFHSYYLSNSYINPRYNSMKIYKY 112
>sp|Q91V57|CHIN_MOUSE N-chimaerin (NC) (N-chimerin) (Alpha chimerin) (A-chimaerin)
(Rho-GTPase-activating protein 2)
Length = 334
Score = 31.2 bits (69), Expect = 3.9
Identities = 18/58 (31%), Positives = 33/58 (56%)
Frame = +3
Query: 375 KFLKWAKSVDIKMLTDAGYKVMCSELNTTESERCREIISHIGTVILHELENVLSVKNV 548
KF++ AK +D + ++ + S L E R +++H+ V LHE EN++S +N+
Sbjct: 237 KFIESAKIMDPDEQLETLHEALRS-LPPAHCETLRYLMAHLKRVTLHEKENLMSAENL 293
>sp|P50436|GLYA_METTM Serine hydroxymethyltransferase (Serine methylase) (SHMT)
Length = 424
Score = 31.2 bits (69), Expect = 3.9
Identities = 33/136 (24%), Positives = 57/136 (41%), Gaps = 2/136 (1%)
Frame = +3
Query: 414 LTDAGYKVMCSELNTTESERCREIISHIGTVILHELENVLSVKNVCYKIYRCPM-NLETT 590
L + G+ V+C L+ TES + +S IG E+ L N+ P ++ +
Sbjct: 296 LNELGFNVLCEHLDFTESHQVVMDVSDIGRAA--EISKKLEANNIILNKNLLPWDDVNRS 353
Query: 591 NEMHSFYYDTFDFTRQTSKRCEVCKAFSDVFSSGID-RDLFEEMVVDQFEKVYCNKLFPK 767
++ T + TR+ K E+ + + ID R++ EE V +F Y P
Sbjct: 354 DDPSGIRIGTQEITRRGMKESEMSEVAEYIKKVVIDGRNVKEE--VSEFMSSYTRSTTP- 410
Query: 768 KEHMCKKFLKNELPTS 815
L+++ PTS
Sbjct: 411 --------LRSQGPTS 418
>sp|P69037|VFP_NPVAC FP protein (25 kDa protein)
sp|P69038|VFP_NPVGM FP protein (25 kDa protein)
Length = 214
Score = 31.2 bits (69), Expect = 3.9
Identities = 13/46 (28%), Positives = 26/46 (56%)
Frame = +1
Query: 88 MNIQHIIINIFMKHHIPKIICFHTNISNISTKIKQQIISHINFSMN 225
+N +H+I + F K+HI +C T TK +++ + + N ++N
Sbjct: 77 LNFKHVIESSFDKNHIVAKLCDATRAKEWQTKSRERRLKNFNLNIN 122
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,899,817
Number of Sequences: 369166
Number of extensions: 2048656
Number of successful extensions: 6106
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5795
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6085
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8550075140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)