Planaria EST Database

DrC_01054

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01054
         (1069 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P90900|IFA4_CAEEL  Intermediate filament protein ifa-4 (I...    89   2e-17
sp|Q19289|IFB1_CAEEL  Intermediate filament protein ifb-1 (I...    79   3e-14
sp|P23731|IFEB_ASCSU  Intermediate filament protein B (IF-B)       75   4e-13
sp|P31732|OV71_ONCVO  Muscle cell intermediate filament prot...    74   9e-13
sp|P23730|IFEA_ASCSU  Intermediate filament protein A (IF-A)       73   1e-12
sp|P90901|IFA1_CAEEL  Intermediate filament protein ifa-1 (I...    73   2e-12
sp|Q17107|AV71_ACAVI  Muscle cell intermediate filament prot...    73   2e-12
sp|P14732|LAM2_CHICK  Lamin B2                                     72   2e-12
sp|O02365|IFA2_CAEEL  Intermediate filament protein ifa-2 (I...    72   3e-12
sp|Q01241|NF70_LOLPE  70 kDa neurofilament protein (NF70)          70   1e-11

>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
            A4) (IF-A4) (Cel IF A4)
          Length = 575

 Score = 89.0 bits (219), Expect = 2e-17
 Identities = 74/287 (25%), Positives = 119/287 (41%), Gaps = 16/287 (5%)
 Frame = +2

Query: 11   MRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQS 190
            MR ++ADLE +N   E  +  +    EE  RS E  L                 + ELQ 
Sbjct: 338  MRGKMADLEGRNLLLEKQIEDLNYQMEEDMRSYEQSLNDKDTSINKLRDESKILMVELQM 397

Query: 191  LLDAKLGLELEIAAYGKLLEFEESRVNSNTEGNMIISQFSNFTAXXXXXXXXXXXXXXXX 370
            L+D K  L+ EI  Y K+L+ EE+R         ++   S                    
Sbjct: 398  LIDTKQTLDAEIVIYRKMLDGEENRAGLRQLVEQVVKTTS-------------------- 437

Query: 371  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYETKKMQSRR--------------TTKGN 508
                                           ++TK+++S R              + KGN
Sbjct: 438  ------------------------------IHQTKEIESLRVLKGETTSHSSYSRSAKGN 467

Query: 509  ISIAESDESGLFIILENMSTKKEILSGMKIKRNIDGKSDSVVFSIPDNTELNGGEKITIW 688
            I+I E++ SG +I+LEN+S + E +   K++R I GK + +VF+ P    L   + + I+
Sbjct: 468  IAIQEAEPSGKYIVLENISRRDENIGDWKLRRKIAGKRE-IVFTFpreFTLRAQKSVKIF 526

Query: 689  ANGKKTGKGKELEIN--EKSWGFGENVVTILVDKEDEEKATLNQEVS 823
            A G+      +  +   E S+G G +VVT L +KE E++A+ +Q  S
Sbjct: 527  ARGQGVHSPPDSLVYDLEDSFGTGNDVVTTLYNKEGEDRASHSQRAS 573

>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
           B1) (IF-B1) (Cel IF B1)
          Length = 589

 Score = 78.6 bits (192), Expect = 3e-14
 Identities = 48/118 (40%), Positives = 72/118 (61%), Gaps = 4/118 (3%)
 Frame = +2

Query: 476 KMQSRRTTKGNISIAESDESGLFIILENMSTKK-EILSGMKIKRNIDGKSDSVVFSIPDN 652
           K   +R+ KGN++I+E D +G FI LEN    K E +   KI+R +DG+ + +V+SIP N
Sbjct: 467 KTTFQRSAKGNVTISECDPNGKFIKLENSHRNKDENVGEHKIRRKLDGRRE-IVYSIPAN 525

Query: 653 TELNGGEKITIWAN--GKKTGKGKELEIN-EKSWGFGENVVTILVDKEDEEKATLNQE 817
             +  G+ +TI+A   G      + L  + E +WG G NVVT LV+K+ EE+AT  Q+
Sbjct: 526 VVIKPGKNLTIYARDQGGINNPPESLVFDGENTWGIGANVVTSLVNKDGEERATHTQK 583


 Score = 50.4 bits (119), Expect = 8e-06
 Identities = 30/89 (33%), Positives = 43/89 (48%)
 Frame = +2

Query: 2   IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
           I  +R ++ DLE +N+  E  +  +     + QR  E  L                 + E
Sbjct: 345 IGDLRGKLGDLENKNSLLEKEVQNLNYQLTDDQRQYEAALNDRDATLRRMREECQTLVAE 404

Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEESRV 268
           LQ+LLD K  L+ EIA Y K+LE EE+RV
Sbjct: 405 LQALLDTKQMLDAEIAIYRKMLEGEETRV 433

>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
          Length = 589

 Score = 74.7 bits (182), Expect = 4e-13
 Identities = 44/118 (37%), Positives = 69/118 (58%), Gaps = 4/118 (3%)
 Frame = +2

Query: 476 KMQSRRTTKGNISIAESDESGLFIILENMS-TKKEILSGMKIKRNIDGKSDSVVFSIPDN 652
           K   +R+ KGN++I+E D +G FI LEN   +K E L   ++KR +D + + +V++IP N
Sbjct: 467 KTTFQRSAKGNVTISECDPNGKFITLENTHRSKDENLGEHRLKRKLDNRRE-IVYTIPPN 525

Query: 653 TELNGGEKITIWAN---GKKTGKGKELEINEKSWGFGENVVTILVDKEDEEKATLNQE 817
           T L  G  + I+A    G        +   E +WG G NVVT L++K+ +E+AT  Q+
Sbjct: 526 TVLKAGRTMKIYARDQGGIHNPPDTLVFDGENTWGIGANVVTSLINKDGDERATHTQK 583


 Score = 52.8 bits (125), Expect = 2e-06
 Identities = 32/89 (35%), Positives = 43/89 (48%)
 Frame = +2

Query: 2   IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
           I  +R ++ DLE +NA  E  +  +     + QR  E  L                 + E
Sbjct: 345 IGDLRGKLGDLEAKNALLEKEVQNLNYQLNDDQRQYEAALNDRDATLRRMREECQTLVAE 404

Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEESRV 268
           LQ+LLD K  L+ EIA Y K+LE EESRV
Sbjct: 405 LQALLDTKQMLDAEIAIYRKMLEGEESRV 433

>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
          Length = 432

 Score = 73.6 bits (179), Expect = 9e-13
 Identities = 44/115 (38%), Positives = 69/115 (60%), Gaps = 3/115 (2%)
 Frame = +2

Query: 488 RRTTKGNISIAESDESGLFIILENM-STKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 664
           +R+ KGN+SI ++   G FI+LEN   +K+E +   ++KR IDGK + +V++ P +  L 
Sbjct: 315 QRSAKGNVSIQDASSDGKFILLENTHRSKEEPIGEWRLKRKIDGKRE-IVYTFPRDFILK 373

Query: 665 GGEKITIWANGKKTGKGKELEI--NEKSWGFGENVVTILVDKEDEEKATLNQEVS 823
            G+ + IWA G+      +  +   E S+G G NV TIL +KE EE+A+  Q  S
Sbjct: 374 PGKTVKIWARGQGVYSPPDQLVFDAEDSFGVGSNVQTILFNKEGEERASHIQRSS 428


 Score = 55.1 bits (131), Expect = 3e-07
 Identities = 32/88 (36%), Positives = 45/88 (51%)
 Frame = +2

Query: 2   IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
           +  +R ++ADLE +N+  E  +  +    E+ QRS E  L               A + E
Sbjct: 189 LSDLRGKLADLEGRNSLLEKQMQELNYQLEDDQRSYEAALNDRDAQIRKMREECQALMME 248

Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEESR 265
           LQ LLD K  L+ EIA Y K+LE EE+R
Sbjct: 249 LQMLLDTKQTLDAEIAIYRKMLEGEENR 276

>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
          Length = 497

 Score = 73.2 bits (178), Expect = 1e-12
 Identities = 44/116 (37%), Positives = 72/116 (62%), Gaps = 4/116 (3%)
 Frame = +2

Query: 488 RRTTKGNISIAESDESGLFIILENM-STKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 664
           +R+ KGN+SI E+   G +++LEN   +K+E +   K+KR IDGK + +V+++P +  L 
Sbjct: 380 QRSAKGNVSIQETAPDGRYVVLENTHRSKEEAIGEWKLKRKIDGKRE-IVYTLPRDFILR 438

Query: 665 GGEKITIWANGKK--TGKGKELEIN-EKSWGFGENVVTILVDKEDEEKATLNQEVS 823
            G+ + IWA G+       ++L  + E ++G G NV TIL ++E EE+AT  Q  S
Sbjct: 439 PGKSVKIWARGQGGIHSPPEQLVFDLEDTFGSGSNVQTILFNREGEERATHIQRSS 494


 Score = 39.3 bits (90), Expect = 0.019
 Identities = 31/90 (34%), Positives = 43/90 (47%), Gaps = 2/90 (2%)
 Frame = +2

Query: 2   IQSMRSRIADLERQNAEYEHNLNMIRGNY--EETQRSLELKLMSXXXXXXXXXXXXSAAL 175
           +  +R ++ADLE +N+  +    +   NY  E+ QRS E  L               A  
Sbjct: 259 LTDLRGKLADLEGRNSLLKQTQEL---NYQLEDDQRSYEAALNDRDAQIRKMDEECQA-- 313

Query: 176 TELQSLLDAKLGLELEIAAYGKLLEFEESR 265
             L  LLD K  L+ EIA Y K+LE EE+R
Sbjct: 314 --LMMLLDTKQTLDAEIAIYRKMLEGEENR 341

>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
           A1) (IF-A1) (Cel IF A1)
          Length = 575

 Score = 72.8 bits (177), Expect = 2e-12
 Identities = 48/123 (39%), Positives = 71/123 (57%), Gaps = 4/123 (3%)
 Frame = +2

Query: 467 ETKKMQS-RRTTKGNISIAESDESGLFIILENMS-TKKEILSGMKIKRNIDGKSDSVVFS 640
           ET   QS +R+ KGN+SI E+   G FI+L+N    K E +   K+KR IDGK ++V ++
Sbjct: 452 ETASRQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRENV-YT 510

Query: 641 IPDNTELNGGEKITIWANGKKTGKGKELEIN--EKSWGFGENVVTILVDKEDEEKATLNQ 814
           +P +  L  G+ + I+A  +      +  +   E S+G G NV TIL +KE EE+AT  Q
Sbjct: 511 LPRDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 570

Query: 815 EVS 823
             S
Sbjct: 571 RQS 573


 Score = 55.8 bits (133), Expect = 2e-07
 Identities = 32/88 (36%), Positives = 45/88 (51%)
 Frame = +2

Query: 2   IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
           +  +R ++ADLE +N+  E  +  +    E+ QRS E  L               A + E
Sbjct: 334 LSDLRGKLADLESRNSLLEKQIQELNYQLEDDQRSYEAALNDRDSQIRKMREECQALMVE 393

Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEESR 265
           LQ LLD K  L+ EIA Y K+LE EE+R
Sbjct: 394 LQMLLDTKQTLDAEIAIYRKMLEGEENR 421

>sp|Q17107|AV71_ACAVI Muscle cell intermediate filament protein AV71
          Length = 394

 Score = 72.8 bits (177), Expect = 2e-12
 Identities = 43/115 (37%), Positives = 68/115 (59%), Gaps = 3/115 (2%)
 Frame = +2

Query: 488 RRTTKGNISIAESDESGLFIILENMS-TKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 664
           +R+ KGN+SI ++   G +I+ EN   +K+E +   ++KR IDGK + +V++ P N  L 
Sbjct: 277 QRSAKGNVSIQDAASDGKYILSENTHRSKEEPIGEWRLKRKIDGKRE-IVYTFPTNFVLK 335

Query: 665 GGEKITIWANGKKTGKGKELEI--NEKSWGFGENVVTILVDKEDEEKATLNQEVS 823
            G+ + IWA G+      +  +   E S+G G NV TIL +KE EE+A+  Q  S
Sbjct: 336 PGKSVKIWARGQGVYSPPDQLVFDAEDSFGIGSNVQTILFNKEGEERASHIQRSS 390


 Score = 53.9 bits (128), Expect = 8e-07
 Identities = 32/88 (36%), Positives = 44/88 (50%)
 Frame = +2

Query: 2   IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
           +  +R ++ADLE +N+  E     +    E+ QRS E  L               A + E
Sbjct: 151 LSELRGKLADLEGRNSLLEKQTQELNYQLEDDQRSYEAALNDRDAQIRKMREECQALMME 210

Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEESR 265
           LQ LLD K  L+ EIA Y K+LE EE+R
Sbjct: 211 LQMLLDTKQTLDAEIAIYRKMLEGEENR 238

>sp|P14732|LAM2_CHICK Lamin B2
          Length = 600

 Score = 72.4 bits (176), Expect = 2e-12
 Identities = 61/272 (22%), Positives = 109/272 (40%), Gaps = 5/272 (1%)
 Frame = +2

Query: 2    IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
            I+S+  +++ L++Q +  E  +  ++      +      L +               LTE
Sbjct: 293  IESLSHQLSGLQKQASATEDRIRELKETMAGERDKFRKMLDAKEREMTEMRDQMQLQLTE 352

Query: 182  LQSLLDAKLGLELEIAAYGKLLEFEES--RVNSNTEGNMIISQFSNFTAXXXXXXXXXXX 355
             Q LLD KL L++EI+AY KLLE EE   +++ +    + +S+ ++ ++           
Sbjct: 353  YQELLDVKLALDMEISAYRKLLEGEEERLKLSPSPSSRVTVSRATSSSSSSSTSLVRSSR 412

Query: 356  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYETKKMQSRRTTKGNISIAESDES 535
                                                  + +M  + +  G+ISI E D  
Sbjct: 413  GKRRRIEAEELSGSGTSGIGTGSISGSSSS-------SSFQMSQQASATGSISIEEIDLE 465

Query: 536  GLFIILENMSTKKEILSGMKIKRNIDGKSDSVVFSIPDNTELNGGEKITIWANGKKTGKG 715
            G ++ L+N S K + L   ++KR I G  + + +       L  G+ +TIW         
Sbjct: 466  GKYVQLKNNSEKDQSLGNWRLKRQI-GDGEEIAYKFTPKYVLRAGQTVTIWGADAGVSHS 524

Query: 716  KELEI---NEKSWGFGENVVTILVDKEDEEKA 802
                +   N+ SWG G N+ T LV+ + EE A
Sbjct: 525  PPSVLVWKNQGSWGTGGNIRTYLVNSDGEEVA 556

>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
           A2) (IF-A2) (Cel IF A2)
          Length = 581

 Score = 72.0 bits (175), Expect = 3e-12
 Identities = 44/116 (37%), Positives = 70/116 (60%), Gaps = 3/116 (2%)
 Frame = +2

Query: 488 RRTTKGNISIAESDESGLFIILENM-STKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 664
           +R+ KGN++I E+   G F+ILEN    K+E L   K+KR IDGK + +VF+ P +  L+
Sbjct: 462 QRSAKGNVAIKETSPEGKFVILENTHRAKEEPLGDWKLKRKIDGKRE-IVFTFPSDYILH 520

Query: 665 GGEKITIWANGKKTGKGKELEI--NEKSWGFGENVVTILVDKEDEEKATLNQEVSK 826
             + + I+A G+      E+ I   ++++G G NV TIL + + EE+AT  Q  S+
Sbjct: 521 PFQSVKIFARGQGIANPPEVLIFEGDETFGVGANVQTILYNNKGEERATHIQRQSQ 576


 Score = 45.1 bits (105), Expect = 4e-04
 Identities = 29/93 (31%), Positives = 43/93 (46%)
 Frame = +2

Query: 11  MRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQS 190
           +R +++DLE +N   E  +  +    E+ QRS E  L               A + ELQ 
Sbjct: 339 LRQKLSDLESRNLLLEKQIEDLNYQLEDDQRSYEAALNDKDAQIRKLREECQALMVELQM 398

Query: 191 LLDAKLGLELEIAAYGKLLEFEESRVNSNTEGN 289
           LLD K  L+ E+  Y ++LE        N+EGN
Sbjct: 399 LLDTKQTLDGELKVYRQMLE-------GNSEGN 424

>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
          Length = 615

 Score = 70.1 bits (170), Expect = 1e-11
 Identities = 46/115 (40%), Positives = 67/115 (58%), Gaps = 2/115 (1%)
 Frame = +2

Query: 476 KMQSRRTTKGNISIAESDESGLFIILENMSTKKEILSGMKIKRNIDGKSDSVVFSIPDNT 655
           K   +RT+KG++SI E+D  G FI LE  + K+E L+G KI R +D  +    + IP N 
Sbjct: 500 KTTYQRTSKGSVSIKEADSQGCFIALE--TKKEENLTGWKIVRKVD-DNKVYTYEIP-NL 555

Query: 656 ELNGGEKITIWA-NGKKTGKGKELEINEK-SWGFGENVVTILVDKEDEEKATLNQ 814
            L  G  + IW+ N +   +G +L   E  +WG G NVVTIL +++ E+KA   Q
Sbjct: 556 VLKTGTVVKIWSKNHQAQARGDDLVSRENDTWGTGSNVVTILQNEKGEDKANYTQ 610


 Score = 65.1 bits (157), Expect = 3e-10
 Identities = 39/86 (45%), Positives = 49/86 (56%)
 Frame = +2

Query: 11  MRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQS 190
           +R+R+ADLE +NA+ E     +  + EE  R  EL+                + L ELQ 
Sbjct: 364 IRNRLADLEARNAQLERTNQDLLRDLEEKDRQNELESCQYKEEITKLRGEMESILKELQD 423

Query: 191 LLDAKLGLELEIAAYGKLLEFEESRV 268
           L+D KL LELEIAAY KLLE EESRV
Sbjct: 424 LMDIKLSLELEIAAYRKLLEGEESRV 449

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,805,478
Number of Sequences: 369166
Number of extensions: 1461393
Number of successful extensions: 4257
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4023
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4201
length of database: 68,354,980
effective HSP length: 112
effective length of database: 47,664,660
effective search space used: 11582512380
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01054

  1. Dr_sW_026_J12
  2. Dr_sW_013_A22
  3. Dr_sW_019_L06