Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_L06
(959 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (I... 79 2e-14
sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B) 75 4e-13
sp|P31732|OV71_ONCVO Muscle cell intermediate filament prot... 74 8e-13
sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (I... 73 1e-12
sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A) 73 1e-12
sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (I... 73 1e-12
sp|Q17107|AV71_ACAVI Muscle cell intermediate filament prot... 73 1e-12
sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (I... 72 2e-12
sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70) 70 9e-12
sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (I... 70 1e-11
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
B1) (IF-B1) (Cel IF B1)
Length = 589
Score = 78.6 bits (192), Expect = 2e-14
Identities = 48/118 (40%), Positives = 72/118 (61%), Gaps = 4/118 (3%)
Frame = +3
Query: 366 KMQSRRTTKGNISIAESDESGLFIILENMSTKK-EILSGMKIKRNIDGKSDSVVFSIPDN 542
K +R+ KGN++I+E D +G FI LEN K E + KI+R +DG+ + +V+SIP N
Sbjct: 467 KTTFQRSAKGNVTISECDPNGKFIKLENSHRNKDENVGEHKIRRKLDGRRE-IVYSIPAN 525
Query: 543 TELNGGEKITIWAN--GKKTGKGKELEIN-EKSWGFGENVVTILVDKEDEEKATLNQE 707
+ G+ +TI+A G + L + E +WG G NVVT LV+K+ EE+AT Q+
Sbjct: 526 VVIKPGKNLTIYARDQGGINNPPESLVFDGENTWGIGANVVTSLVNKDGEERATHTQK 583
Score = 37.4 bits (85), Expect = 0.063
Identities = 19/32 (59%), Positives = 24/32 (75%)
Frame = +3
Query: 63 LTELQSLLDAKLGLELEIAAYGKLLEFEESRV 158
+ ELQ+LLD K L+ EIA Y K+LE EE+RV
Sbjct: 402 VAELQALLDTKQMLDAEIAIYRKMLEGEETRV 433
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
Length = 589
Score = 74.7 bits (182), Expect = 4e-13
Identities = 44/118 (37%), Positives = 69/118 (58%), Gaps = 4/118 (3%)
Frame = +3
Query: 366 KMQSRRTTKGNISIAESDESGLFIILENMS-TKKEILSGMKIKRNIDGKSDSVVFSIPDN 542
K +R+ KGN++I+E D +G FI LEN +K E L ++KR +D + + +V++IP N
Sbjct: 467 KTTFQRSAKGNVTISECDPNGKFITLENTHRSKDENLGEHRLKRKLDNRRE-IVYTIPPN 525
Query: 543 TELNGGEKITIWAN---GKKTGKGKELEINEKSWGFGENVVTILVDKEDEEKATLNQE 707
T L G + I+A G + E +WG G NVVT L++K+ +E+AT Q+
Sbjct: 526 TVLKAGRTMKIYARDQGGIHNPPDTLVFDGENTWGIGANVVTSLINKDGDERATHTQK 583
Score = 38.5 bits (88), Expect = 0.028
Identities = 20/32 (62%), Positives = 24/32 (75%)
Frame = +3
Query: 63 LTELQSLLDAKLGLELEIAAYGKLLEFEESRV 158
+ ELQ+LLD K L+ EIA Y K+LE EESRV
Sbjct: 402 VAELQALLDTKQMLDAEIAIYRKMLEGEESRV 433
>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
Length = 432
Score = 73.6 bits (179), Expect = 8e-13
Identities = 44/115 (38%), Positives = 69/115 (60%), Gaps = 3/115 (2%)
Frame = +3
Query: 378 RRTTKGNISIAESDESGLFIILENM-STKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 554
+R+ KGN+SI ++ G FI+LEN +K+E + ++KR IDGK + +V++ P + L
Sbjct: 315 QRSAKGNVSIQDASSDGKFILLENTHRSKEEPIGEWRLKRKIDGKRE-IVYTFPRDFILK 373
Query: 555 GGEKITIWANGKKTGKGKELEI--NEKSWGFGENVVTILVDKEDEEKATLNQEVS 713
G+ + IWA G+ + + E S+G G NV TIL +KE EE+A+ Q S
Sbjct: 374 PGKTVKIWARGQGVYSPPDQLVFDAEDSFGVGSNVQTILFNKEGEERASHIQRSS 428
Score = 36.6 bits (83), Expect = 0.11
Identities = 19/33 (57%), Positives = 23/33 (69%)
Frame = +3
Query: 57 AALTELQSLLDAKLGLELEIAAYGKLLEFEESR 155
A + ELQ LLD K L+ EIA Y K+LE EE+R
Sbjct: 244 ALMMELQMLLDTKQTLDAEIAIYRKMLEGEENR 276
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
A4) (IF-A4) (Cel IF A4)
Length = 575
Score = 73.2 bits (178), Expect = 1e-12
Identities = 60/233 (25%), Positives = 100/233 (42%), Gaps = 16/233 (6%)
Frame = +3
Query: 63 LTELQSLLDAKLGLELEIAAYGKLLEFEESRVNSNTEGNMIISQFSNFTAXXXXXXXXXX 242
+ ELQ L+D K L+ EI Y K+L+ EE+R ++ S
Sbjct: 392 MVELQMLIDTKQTLDAEIVIYRKMLDGEENRAGLRQLVEQVVKTTS-------------- 437
Query: 243 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYETKKMQSRR------------- 383
++TK+++S R
Sbjct: 438 ------------------------------------IHQTKEIESLRVLKGETTSHSSYS 461
Query: 384 -TTKGNISIAESDESGLFIILENMSTKKEILSGMKIKRNIDGKSDSVVFSIPDNTELNGG 560
+ KGNI+I E++ SG +I+LEN+S + E + K++R I GK + +VF+ P L
Sbjct: 462 RSAKGNIAIQEAEPSGKYIVLENISRRDENIGDWKLRRKIAGKRE-IVFTFpreFTLRAQ 520
Query: 561 EKITIWANGKKTGKGKELEIN--EKSWGFGENVVTILVDKEDEEKATLNQEVS 713
+ + I+A G+ + + E S+G G +VVT L +KE E++A+ +Q S
Sbjct: 521 KSVKIFARGQGVHSPPDSLVYDLEDSFGTGNDVVTTLYNKEGEDRASHSQRAS 573
>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
Length = 497
Score = 73.2 bits (178), Expect = 1e-12
Identities = 44/116 (37%), Positives = 72/116 (62%), Gaps = 4/116 (3%)
Frame = +3
Query: 378 RRTTKGNISIAESDESGLFIILENM-STKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 554
+R+ KGN+SI E+ G +++LEN +K+E + K+KR IDGK + +V+++P + L
Sbjct: 380 QRSAKGNVSIQETAPDGRYVVLENTHRSKEEAIGEWKLKRKIDGKRE-IVYTLPRDFILR 438
Query: 555 GGEKITIWANGKK--TGKGKELEIN-EKSWGFGENVVTILVDKEDEEKATLNQEVS 713
G+ + IWA G+ ++L + E ++G G NV TIL ++E EE+AT Q S
Sbjct: 439 PGKSVKIWARGQGGIHSPPEQLVFDLEDTFGSGSNVQTILFNREGEERATHIQRSS 494
Score = 31.2 bits (69), Expect = 4.5
Identities = 16/28 (57%), Positives = 19/28 (67%)
Frame = +3
Query: 72 LQSLLDAKLGLELEIAAYGKLLEFEESR 155
L LLD K L+ EIA Y K+LE EE+R
Sbjct: 314 LMMLLDTKQTLDAEIAIYRKMLEGEENR 341
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
A1) (IF-A1) (Cel IF A1)
Length = 575
Score = 72.8 bits (177), Expect = 1e-12
Identities = 48/123 (39%), Positives = 71/123 (57%), Gaps = 4/123 (3%)
Frame = +3
Query: 357 ETKKMQS-RRTTKGNISIAESDESGLFIILENMS-TKKEILSGMKIKRNIDGKSDSVVFS 530
ET QS +R+ KGN+SI E+ G FI+L+N K E + K+KR IDGK ++V ++
Sbjct: 452 ETASRQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRENV-YT 510
Query: 531 IPDNTELNGGEKITIWANGKKTGKGKELEIN--EKSWGFGENVVTILVDKEDEEKATLNQ 704
+P + L G+ + I+A + + + E S+G G NV TIL +KE EE+AT Q
Sbjct: 511 LPRDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 570
Query: 705 EVS 713
S
Sbjct: 571 RQS 573
Score = 37.0 bits (84), Expect = 0.082
Identities = 19/33 (57%), Positives = 23/33 (69%)
Frame = +3
Query: 57 AALTELQSLLDAKLGLELEIAAYGKLLEFEESR 155
A + ELQ LLD K L+ EIA Y K+LE EE+R
Sbjct: 389 ALMVELQMLLDTKQTLDAEIAIYRKMLEGEENR 421
>sp|Q17107|AV71_ACAVI Muscle cell intermediate filament protein AV71
Length = 394
Score = 72.8 bits (177), Expect = 1e-12
Identities = 43/115 (37%), Positives = 68/115 (59%), Gaps = 3/115 (2%)
Frame = +3
Query: 378 RRTTKGNISIAESDESGLFIILENMS-TKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 554
+R+ KGN+SI ++ G +I+ EN +K+E + ++KR IDGK + +V++ P N L
Sbjct: 277 QRSAKGNVSIQDAASDGKYILSENTHRSKEEPIGEWRLKRKIDGKRE-IVYTFPTNFVLK 335
Query: 555 GGEKITIWANGKKTGKGKELEI--NEKSWGFGENVVTILVDKEDEEKATLNQEVS 713
G+ + IWA G+ + + E S+G G NV TIL +KE EE+A+ Q S
Sbjct: 336 PGKSVKIWARGQGVYSPPDQLVFDAEDSFGIGSNVQTILFNKEGEERASHIQRSS 390
Score = 36.6 bits (83), Expect = 0.11
Identities = 19/33 (57%), Positives = 23/33 (69%)
Frame = +3
Query: 57 AALTELQSLLDAKLGLELEIAAYGKLLEFEESR 155
A + ELQ LLD K L+ EIA Y K+LE EE+R
Sbjct: 206 ALMMELQMLLDTKQTLDAEIAIYRKMLEGEENR 238
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
A2) (IF-A2) (Cel IF A2)
Length = 581
Score = 72.0 bits (175), Expect = 2e-12
Identities = 44/116 (37%), Positives = 70/116 (60%), Gaps = 3/116 (2%)
Frame = +3
Query: 378 RRTTKGNISIAESDESGLFIILENM-STKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 554
+R+ KGN++I E+ G F+ILEN K+E L K+KR IDGK + +VF+ P + L+
Sbjct: 462 QRSAKGNVAIKETSPEGKFVILENTHRAKEEPLGDWKLKRKIDGKRE-IVFTFPSDYILH 520
Query: 555 GGEKITIWANGKKTGKGKELEI--NEKSWGFGENVVTILVDKEDEEKATLNQEVSK 716
+ + I+A G+ E+ I ++++G G NV TIL + + EE+AT Q S+
Sbjct: 521 PFQSVKIFARGQGIANPPEVLIFEGDETFGVGANVQTILYNNKGEERATHIQRQSQ 576
>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
Length = 615
Score = 70.1 bits (170), Expect = 9e-12
Identities = 46/115 (40%), Positives = 67/115 (58%), Gaps = 2/115 (1%)
Frame = +3
Query: 366 KMQSRRTTKGNISIAESDESGLFIILENMSTKKEILSGMKIKRNIDGKSDSVVFSIPDNT 545
K +RT+KG++SI E+D G FI LE + K+E L+G KI R +D + + IP N
Sbjct: 500 KTTYQRTSKGSVSIKEADSQGCFIALE--TKKEENLTGWKIVRKVD-DNKVYTYEIP-NL 555
Query: 546 ELNGGEKITIWA-NGKKTGKGKELEINEK-SWGFGENVVTILVDKEDEEKATLNQ 704
L G + IW+ N + +G +L E +WG G NVVTIL +++ E+KA Q
Sbjct: 556 VLKTGTVVKIWSKNHQAQARGDDLVSRENDTWGTGSNVVTILQNEKGEDKANYTQ 610
Score = 47.0 bits (110), Expect = 8e-05
Identities = 25/32 (78%), Positives = 26/32 (81%)
Frame = +3
Query: 63 LTELQSLLDAKLGLELEIAAYGKLLEFEESRV 158
L ELQ L+D KL LELEIAAY KLLE EESRV
Sbjct: 418 LKELQDLMDIKLSLELEIAAYRKLLEGEESRV 449
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
A3) (IF-A3) (Cel IF A3)
Length = 581
Score = 69.7 bits (169), Expect = 1e-11
Identities = 44/116 (37%), Positives = 66/116 (56%), Gaps = 3/116 (2%)
Frame = +3
Query: 378 RRTTKGNISIAESDESGLFIILENM-STKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 554
+R+ KGN+SI E G F+ILEN K+E L K+KR IDGK + +VF+ P + L+
Sbjct: 462 QRSAKGNVSIKEVSPEGKFVILENTHRDKEEPLGDWKLKRKIDGKRE-IVFTFPSDYILH 520
Query: 555 GGEKITIWANGKKTGKGKELEI--NEKSWGFGENVVTILVDKEDEEKATLNQEVSK 716
+ + I+A G E+ + + ++G G NV TIL + EE+AT Q S+
Sbjct: 521 PVQTVKIFARGNGVANPPEVLVFEGDDTFGAGANVQTILYNNSGEERATHMQRQSQ 576
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,902,717
Number of Sequences: 369166
Number of extensions: 1316517
Number of successful extensions: 3703
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3562
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3680
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 9952674160
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)