Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_J12
(868 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (I... 89 2e-17
sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (I... 79 2e-14
sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B) 75 3e-13
sp|P31732|OV71_ONCVO Muscle cell intermediate filament prot... 74 7e-13
sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A) 73 9e-13
sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (I... 73 1e-12
sp|Q17107|AV71_ACAVI Muscle cell intermediate filament prot... 73 1e-12
sp|P14732|LAM2_CHICK Lamin B2 72 2e-12
sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (I... 72 2e-12
sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70) 70 8e-12
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
A4) (IF-A4) (Cel IF A4)
Length = 575
Score = 89.0 bits (219), Expect = 2e-17
Identities = 74/287 (25%), Positives = 119/287 (41%), Gaps = 16/287 (5%)
Frame = +2
Query: 11 MRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQS 190
MR ++ADLE +N E + + EE RS E L + ELQ
Sbjct: 338 MRGKMADLEGRNLLLEKQIEDLNYQMEEDMRSYEQSLNDKDTSINKLRDESKILMVELQM 397
Query: 191 LLDAKLGLELEIAAYGKLLEFEESRVNSNTEGNMIISQFSNFTAXXXXXXXXXXXXXXXX 370
L+D K L+ EI Y K+L+ EE+R ++ S
Sbjct: 398 LIDTKQTLDAEIVIYRKMLDGEENRAGLRQLVEQVVKTTS-------------------- 437
Query: 371 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYETKKMQSRR--------------TTKGN 508
++TK+++S R + KGN
Sbjct: 438 ------------------------------IHQTKEIESLRVLKGETTSHSSYSRSAKGN 467
Query: 509 ISIAESDESGLFIILENMSTKKEILSGMKIKRNIDGKSDSVVFSIPDNTELNGGEKITIW 688
I+I E++ SG +I+LEN+S + E + K++R I GK + +VF+ P L + + I+
Sbjct: 468 IAIQEAEPSGKYIVLENISRRDENIGDWKLRRKIAGKRE-IVFTFpreFTLRAQKSVKIF 526
Query: 689 ANGKKTGKGKELEIN--EKSWGFGENVVTILVDKEDEEKATLNQEVS 823
A G+ + + E S+G G +VVT L +KE E++A+ +Q S
Sbjct: 527 ARGQGVHSPPDSLVYDLEDSFGTGNDVVTTLYNKEGEDRASHSQRAS 573
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
B1) (IF-B1) (Cel IF B1)
Length = 589
Score = 78.6 bits (192), Expect = 2e-14
Identities = 48/118 (40%), Positives = 72/118 (61%), Gaps = 4/118 (3%)
Frame = +2
Query: 476 KMQSRRTTKGNISIAESDESGLFIILENMSTKK-EILSGMKIKRNIDGKSDSVVFSIPDN 652
K +R+ KGN++I+E D +G FI LEN K E + KI+R +DG+ + +V+SIP N
Sbjct: 467 KTTFQRSAKGNVTISECDPNGKFIKLENSHRNKDENVGEHKIRRKLDGRRE-IVYSIPAN 525
Query: 653 TELNGGEKITIWAN--GKKTGKGKELEIN-EKSWGFGENVVTILVDKEDEEKATLNQE 817
+ G+ +TI+A G + L + E +WG G NVVT LV+K+ EE+AT Q+
Sbjct: 526 VVIKPGKNLTIYARDQGGINNPPESLVFDGENTWGIGANVVTSLVNKDGEERATHTQK 583
Score = 50.4 bits (119), Expect = 6e-06
Identities = 30/89 (33%), Positives = 43/89 (48%)
Frame = +2
Query: 2 IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
I +R ++ DLE +N+ E + + + QR E L + E
Sbjct: 345 IGDLRGKLGDLENKNSLLEKEVQNLNYQLTDDQRQYEAALNDRDATLRRMREECQTLVAE 404
Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEESRV 268
LQ+LLD K L+ EIA Y K+LE EE+RV
Sbjct: 405 LQALLDTKQMLDAEIAIYRKMLEGEETRV 433
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
Length = 589
Score = 74.7 bits (182), Expect = 3e-13
Identities = 44/118 (37%), Positives = 69/118 (58%), Gaps = 4/118 (3%)
Frame = +2
Query: 476 KMQSRRTTKGNISIAESDESGLFIILENMS-TKKEILSGMKIKRNIDGKSDSVVFSIPDN 652
K +R+ KGN++I+E D +G FI LEN +K E L ++KR +D + + +V++IP N
Sbjct: 467 KTTFQRSAKGNVTISECDPNGKFITLENTHRSKDENLGEHRLKRKLDNRRE-IVYTIPPN 525
Query: 653 TELNGGEKITIWAN---GKKTGKGKELEINEKSWGFGENVVTILVDKEDEEKATLNQE 817
T L G + I+A G + E +WG G NVVT L++K+ +E+AT Q+
Sbjct: 526 TVLKAGRTMKIYARDQGGIHNPPDTLVFDGENTWGIGANVVTSLINKDGDERATHTQK 583
Score = 52.8 bits (125), Expect = 1e-06
Identities = 32/89 (35%), Positives = 43/89 (48%)
Frame = +2
Query: 2 IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
I +R ++ DLE +NA E + + + QR E L + E
Sbjct: 345 IGDLRGKLGDLEAKNALLEKEVQNLNYQLNDDQRQYEAALNDRDATLRRMREECQTLVAE 404
Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEESRV 268
LQ+LLD K L+ EIA Y K+LE EESRV
Sbjct: 405 LQALLDTKQMLDAEIAIYRKMLEGEESRV 433
>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
Length = 432
Score = 73.6 bits (179), Expect = 7e-13
Identities = 44/115 (38%), Positives = 69/115 (60%), Gaps = 3/115 (2%)
Frame = +2
Query: 488 RRTTKGNISIAESDESGLFIILENM-STKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 664
+R+ KGN+SI ++ G FI+LEN +K+E + ++KR IDGK + +V++ P + L
Sbjct: 315 QRSAKGNVSIQDASSDGKFILLENTHRSKEEPIGEWRLKRKIDGKRE-IVYTFPRDFILK 373
Query: 665 GGEKITIWANGKKTGKGKELEI--NEKSWGFGENVVTILVDKEDEEKATLNQEVS 823
G+ + IWA G+ + + E S+G G NV TIL +KE EE+A+ Q S
Sbjct: 374 PGKTVKIWARGQGVYSPPDQLVFDAEDSFGVGSNVQTILFNKEGEERASHIQRSS 428
Score = 55.1 bits (131), Expect = 3e-07
Identities = 32/88 (36%), Positives = 45/88 (51%)
Frame = +2
Query: 2 IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
+ +R ++ADLE +N+ E + + E+ QRS E L A + E
Sbjct: 189 LSDLRGKLADLEGRNSLLEKQMQELNYQLEDDQRSYEAALNDRDAQIRKMREECQALMME 248
Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEESR 265
LQ LLD K L+ EIA Y K+LE EE+R
Sbjct: 249 LQMLLDTKQTLDAEIAIYRKMLEGEENR 276
>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
Length = 497
Score = 73.2 bits (178), Expect = 9e-13
Identities = 44/116 (37%), Positives = 72/116 (62%), Gaps = 4/116 (3%)
Frame = +2
Query: 488 RRTTKGNISIAESDESGLFIILENM-STKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 664
+R+ KGN+SI E+ G +++LEN +K+E + K+KR IDGK + +V+++P + L
Sbjct: 380 QRSAKGNVSIQETAPDGRYVVLENTHRSKEEAIGEWKLKRKIDGKRE-IVYTLPRDFILR 438
Query: 665 GGEKITIWANGKK--TGKGKELEIN-EKSWGFGENVVTILVDKEDEEKATLNQEVS 823
G+ + IWA G+ ++L + E ++G G NV TIL ++E EE+AT Q S
Sbjct: 439 PGKSVKIWARGQGGIHSPPEQLVFDLEDTFGSGSNVQTILFNREGEERATHIQRSS 494
Score = 39.3 bits (90), Expect = 0.014
Identities = 31/90 (34%), Positives = 43/90 (47%), Gaps = 2/90 (2%)
Frame = +2
Query: 2 IQSMRSRIADLERQNAEYEHNLNMIRGNY--EETQRSLELKLMSXXXXXXXXXXXXSAAL 175
+ +R ++ADLE +N+ + + NY E+ QRS E L A
Sbjct: 259 LTDLRGKLADLEGRNSLLKQTQEL---NYQLEDDQRSYEAALNDRDAQIRKMDEECQA-- 313
Query: 176 TELQSLLDAKLGLELEIAAYGKLLEFEESR 265
L LLD K L+ EIA Y K+LE EE+R
Sbjct: 314 --LMMLLDTKQTLDAEIAIYRKMLEGEENR 341
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
A1) (IF-A1) (Cel IF A1)
Length = 575
Score = 72.8 bits (177), Expect = 1e-12
Identities = 48/123 (39%), Positives = 71/123 (57%), Gaps = 4/123 (3%)
Frame = +2
Query: 467 ETKKMQS-RRTTKGNISIAESDESGLFIILENMS-TKKEILSGMKIKRNIDGKSDSVVFS 640
ET QS +R+ KGN+SI E+ G FI+L+N K E + K+KR IDGK ++V ++
Sbjct: 452 ETASRQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRENV-YT 510
Query: 641 IPDNTELNGGEKITIWANGKKTGKGKELEIN--EKSWGFGENVVTILVDKEDEEKATLNQ 814
+P + L G+ + I+A + + + E S+G G NV TIL +KE EE+AT Q
Sbjct: 511 LPRDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 570
Query: 815 EVS 823
S
Sbjct: 571 RQS 573
Score = 55.8 bits (133), Expect = 1e-07
Identities = 32/88 (36%), Positives = 45/88 (51%)
Frame = +2
Query: 2 IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
+ +R ++ADLE +N+ E + + E+ QRS E L A + E
Sbjct: 334 LSDLRGKLADLESRNSLLEKQIQELNYQLEDDQRSYEAALNDRDSQIRKMREECQALMVE 393
Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEESR 265
LQ LLD K L+ EIA Y K+LE EE+R
Sbjct: 394 LQMLLDTKQTLDAEIAIYRKMLEGEENR 421
>sp|Q17107|AV71_ACAVI Muscle cell intermediate filament protein AV71
Length = 394
Score = 72.8 bits (177), Expect = 1e-12
Identities = 43/115 (37%), Positives = 68/115 (59%), Gaps = 3/115 (2%)
Frame = +2
Query: 488 RRTTKGNISIAESDESGLFIILENMS-TKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 664
+R+ KGN+SI ++ G +I+ EN +K+E + ++KR IDGK + +V++ P N L
Sbjct: 277 QRSAKGNVSIQDAASDGKYILSENTHRSKEEPIGEWRLKRKIDGKRE-IVYTFPTNFVLK 335
Query: 665 GGEKITIWANGKKTGKGKELEI--NEKSWGFGENVVTILVDKEDEEKATLNQEVS 823
G+ + IWA G+ + + E S+G G NV TIL +KE EE+A+ Q S
Sbjct: 336 PGKSVKIWARGQGVYSPPDQLVFDAEDSFGIGSNVQTILFNKEGEERASHIQRSS 390
Score = 53.9 bits (128), Expect = 6e-07
Identities = 32/88 (36%), Positives = 44/88 (50%)
Frame = +2
Query: 2 IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
+ +R ++ADLE +N+ E + E+ QRS E L A + E
Sbjct: 151 LSELRGKLADLEGRNSLLEKQTQELNYQLEDDQRSYEAALNDRDAQIRKMREECQALMME 210
Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEESR 265
LQ LLD K L+ EIA Y K+LE EE+R
Sbjct: 211 LQMLLDTKQTLDAEIAIYRKMLEGEENR 238
>sp|P14732|LAM2_CHICK Lamin B2
Length = 600
Score = 72.4 bits (176), Expect = 2e-12
Identities = 61/272 (22%), Positives = 109/272 (40%), Gaps = 5/272 (1%)
Frame = +2
Query: 2 IQSMRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTE 181
I+S+ +++ L++Q + E + ++ + L + LTE
Sbjct: 293 IESLSHQLSGLQKQASATEDRIRELKETMAGERDKFRKMLDAKEREMTEMRDQMQLQLTE 352
Query: 182 LQSLLDAKLGLELEIAAYGKLLEFEES--RVNSNTEGNMIISQFSNFTAXXXXXXXXXXX 355
Q LLD KL L++EI+AY KLLE EE +++ + + +S+ ++ ++
Sbjct: 353 YQELLDVKLALDMEISAYRKLLEGEEERLKLSPSPSSRVTVSRATSSSSSSSTSLVRSSR 412
Query: 356 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYETKKMQSRRTTKGNISIAESDES 535
+ +M + + G+ISI E D
Sbjct: 413 GKRRRIEAEELSGSGTSGIGTGSISGSSSS-------SSFQMSQQASATGSISIEEIDLE 465
Query: 536 GLFIILENMSTKKEILSGMKIKRNIDGKSDSVVFSIPDNTELNGGEKITIWANGKKTGKG 715
G ++ L+N S K + L ++KR I G + + + L G+ +TIW
Sbjct: 466 GKYVQLKNNSEKDQSLGNWRLKRQI-GDGEEIAYKFTPKYVLRAGQTVTIWGADAGVSHS 524
Query: 716 KELEI---NEKSWGFGENVVTILVDKEDEEKA 802
+ N+ SWG G N+ T LV+ + EE A
Sbjct: 525 PPSVLVWKNQGSWGTGGNIRTYLVNSDGEEVA 556
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
A2) (IF-A2) (Cel IF A2)
Length = 581
Score = 72.0 bits (175), Expect = 2e-12
Identities = 44/116 (37%), Positives = 70/116 (60%), Gaps = 3/116 (2%)
Frame = +2
Query: 488 RRTTKGNISIAESDESGLFIILENM-STKKEILSGMKIKRNIDGKSDSVVFSIPDNTELN 664
+R+ KGN++I E+ G F+ILEN K+E L K+KR IDGK + +VF+ P + L+
Sbjct: 462 QRSAKGNVAIKETSPEGKFVILENTHRAKEEPLGDWKLKRKIDGKRE-IVFTFPSDYILH 520
Query: 665 GGEKITIWANGKKTGKGKELEI--NEKSWGFGENVVTILVDKEDEEKATLNQEVSK 826
+ + I+A G+ E+ I ++++G G NV TIL + + EE+AT Q S+
Sbjct: 521 PFQSVKIFARGQGIANPPEVLIFEGDETFGVGANVQTILYNNKGEERATHIQRQSQ 576
Score = 45.1 bits (105), Expect = 3e-04
Identities = 29/93 (31%), Positives = 43/93 (46%)
Frame = +2
Query: 11 MRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQS 190
+R +++DLE +N E + + E+ QRS E L A + ELQ
Sbjct: 339 LRQKLSDLESRNLLLEKQIEDLNYQLEDDQRSYEAALNDKDAQIRKLREECQALMVELQM 398
Query: 191 LLDAKLGLELEIAAYGKLLEFEESRVNSNTEGN 289
LLD K L+ E+ Y ++LE N+EGN
Sbjct: 399 LLDTKQTLDGELKVYRQMLE-------GNSEGN 424
>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
Length = 615
Score = 70.1 bits (170), Expect = 8e-12
Identities = 46/115 (40%), Positives = 67/115 (58%), Gaps = 2/115 (1%)
Frame = +2
Query: 476 KMQSRRTTKGNISIAESDESGLFIILENMSTKKEILSGMKIKRNIDGKSDSVVFSIPDNT 655
K +RT+KG++SI E+D G FI LE + K+E L+G KI R +D + + IP N
Sbjct: 500 KTTYQRTSKGSVSIKEADSQGCFIALE--TKKEENLTGWKIVRKVD-DNKVYTYEIP-NL 555
Query: 656 ELNGGEKITIWA-NGKKTGKGKELEINEK-SWGFGENVVTILVDKEDEEKATLNQ 814
L G + IW+ N + +G +L E +WG G NVVTIL +++ E+KA Q
Sbjct: 556 VLKTGTVVKIWSKNHQAQARGDDLVSRENDTWGTGSNVVTILQNEKGEDKANYTQ 610
Score = 65.1 bits (157), Expect = 2e-10
Identities = 39/86 (45%), Positives = 49/86 (56%)
Frame = +2
Query: 11 MRSRIADLERQNAEYEHNLNMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQS 190
+R+R+ADLE +NA+ E + + EE R EL+ + L ELQ
Sbjct: 364 IRNRLADLEARNAQLERTNQDLLRDLEEKDRQNELESCQYKEEITKLRGEMESILKELQD 423
Query: 191 LLDAKLGLELEIAAYGKLLEFEESRV 268
L+D KL LELEIAAY KLLE EESRV
Sbjct: 424 LMDIKLSLELEIAAYRKLLEGEESRV 449
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,309,521
Number of Sequences: 369166
Number of extensions: 1107588
Number of successful extensions: 3742
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3686
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8550075140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)