Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= DrC_01053 (837 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|O14359|YB4E_SCHPO Hypothetical protein C30D10.14 in chro... 75 3e-13 sp|Q9ZT66|E134_MAIZE Endo-1,3;1,4-beta-D-glucanase precursor 56 1e-07 sp|P0A115|CLCD_PSESB Carboxymethylenebutenolidase (Dienelac... 47 5e-05 sp|P27136|TFDE1_RALEJ Carboxymethylenebutenolidase I (Diene... 39 0.014 sp|P73163|DLHH_SYNY3 Putative carboxymethylenebutenolidase ... 39 0.018 sp|P71505|DLHH_METEX Putative carboxymethylenebutenolidase ... 38 0.030 sp|P27100|TCBE_PSESQ Carboxymethylenebutenolidase (Dienelac... 36 0.12 sp|P39721|YAE9_YEAST Hypothetical 27.1 kDa protein in ACS1-... 36 0.12 sp|Q8Z3B8|DLHH_SALTI Putative carboxymethylenebutenolidase ... 35 0.20 sp|P56262|DLHH_ECOLI Putative carboxymethylenebutenolidase ... 35 0.20
>sp|O14359|YB4E_SCHPO Hypothetical protein C30D10.14 in chromosome II Length = 249 Score = 74.7 bits (182), Expect = 3e-13 Identities = 68/250 (27%), Positives = 103/250 (41%), Gaps = 17/250 (6%) Frame = +3 Query: 48 CCTSGQPQC---DKYTSVGTEIDTSKDFKLYQVGSGNKNGVIIVY-DIFGFNIGQTRRFA 215 CC + + YT G EI+ Y VGS + V+I + DIFG + Q + A Sbjct: 4 CCPTSRGAAASNKSYTFKGKEIENFGGLTTYVVGSTSNTRVLIGFMDIFGLS-DQIKEGA 62 Query: 216 DSLAELAFNVVMPDIFRGKPWTLENFPPKNSGE-------FMKWLNEAGAYSLVEKDLVI 374 D LA+ F V +PD GKP + PPK + F ++ + + K + Sbjct: 63 DKLADDGFTVYLPDFLEGKPLPVTALPPKTPEDQKLCNDFFSTRISPNLHWPKLAKVVEA 122 Query: 375 AREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVSVHGAGISEELVNDIKVPMMF 554 R +G GFCWG K+ T + S H + ++ P++F Sbjct: 123 VRANHGPNVTIG---TYGFCWGAKVLVTYPATIDFVGIASCHPSFPDSADAANVHCPVLF 179 Query: 555 LPAGDDPC--LDSVEKLLQSKPIGKDCVFHRFDKMTHGFAAARGDWNNEETR----DAVC 716 L + D+ + E+ ++ P F F M HG+ AAR D +N E R + Sbjct: 180 LCSKDEDAKIIKEWEEAFKTNPAYAKSSFETFSDMFHGWMAARADLSNPEQRKRFDEGYQ 239 Query: 717 KVISLAQNFM 746 KV S Q+ M Sbjct: 240 KVSSFFQSLM 249
>sp|Q9ZT66|E134_MAIZE Endo-1,3;1,4-beta-D-glucanase precursor Length = 303 Score = 56.2 bits (134), Expect = 1e-07 Identities = 39/146 (26%), Positives = 68/146 (46%), Gaps = 2/146 (1%) Frame = +3 Query: 141 SGNKNGVIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLENFPPKNSGEFM 320 + + V++ D+FG+ R+ D +A+ + VV+PD +G + K + F Sbjct: 65 ASSSRAVVLASDVFGYEAPLLRQIVDKVAKAGYFVVVPDFLKG-----DYLDDKKN--FT 117 Query: 321 KWLNEAGAYSLVE--KDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVS 494 +WL E K L A ++ K+ AVG G+CWGGK+S +TS Sbjct: 118 EWLEAHSPVKAAEDAKPLFAALKKEGKSVAVG-----GYCWGGKLSVEVGKTSDVKAVCL 172 Query: 495 VHGAGISEELVNDIKVPMMFLPAGDD 572 H ++ + + ++K P+ L A +D Sbjct: 173 SHPYSVTADDMKEVKWPIEILGAQND 198
>sp|P0A115|CLCD_PSESB Carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH) sp|P0A114|CLCD_PSEPU Carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH) Length = 236 Score = 47.4 bits (111), Expect = 5e-05 Identities = 46/180 (25%), Positives = 79/180 (43%), Gaps = 10/180 (5%) Frame = +3 Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF-RGKPWTLENFPPKNSGE--FMKWL 329 ++I +IFG N R L + + V PD++ R P T + + E + W Sbjct: 31 IVIAQEIFGVNAFM-RETVSWLVDQGYAAVCPDLYARQAPGTALDPQDERQREQAYKLWQ 89 Query: 330 N---EAGAYSLVEKDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVSVH 500 EAG L E + AR Q G VG ++G+C GG ++ V + Sbjct: 90 AFDMEAGVGDL-EAAIRYARHQPYSNGKVG---LVGYCLGGALAFLVAAKGYVDRAVGYY 145 Query: 501 GAGISEEL--VNDIKVPMMFLPAGDDPCLDSVEKLLQSKPIGKDCVF--HRFDKMTHGFA 668 G G+ ++L V ++K P +F G D + + + L ++ G + + H +++ H FA Sbjct: 146 GVGLEKQLKKVPEVKHPALFHMGGQDHFVPAPSRQLITEGFGANPLLQVHWYEEAGHSFA 205
>sp|P27136|TFDE1_RALEJ Carboxymethylenebutenolidase I (Dienelactone hydrolase I) (DLH I) Length = 234 Score = 39.3 bits (90), Expect = 0.014 Identities = 39/141 (27%), Positives = 61/141 (43%), Gaps = 9/141 (6%) Frame = +3 Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLE---NFPPKNSGE---FM 320 V+I +IFG R + L F V PD++ + +E N P + F Sbjct: 31 VVIAQEIFGIT-PFIRETVEWLVGAGFGCVCPDLYWRQAPNIELDANVPSEREQALALFR 89 Query: 321 KWLNEAGAYSLVEKDLVIAREQLQKTG-AVGKPAILGFCWGGKMSTCACQTSLYSCGVSV 497 + EAG DL A E + + G+ A++G+C GG ++ SL C + Sbjct: 90 DFDMEAGV-----NDLSCAIEYARALPFSNGRVAVVGYCLGGALAFDVAARSLADCSIGY 144 Query: 498 HGAGISEE--LVNDIKVPMMF 554 +G G+ ++ LV I P MF Sbjct: 145 YGVGLEKKVSLVPAITRPAMF 165
>sp|P73163|DLHH_SYNY3 Putative carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH) Length = 246 Score = 38.9 bits (89), Expect = 0.018 Identities = 50/222 (22%), Positives = 82/222 (36%), Gaps = 21/222 (9%) Frame = +3 Query: 135 VGSGNKNGVIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF-RGKPWTLENFPPKNSG 311 V G VI++ +IFG N R + +A+ + + P I+ R P E + P+ Sbjct: 27 VAVGQYPVVIVIQEIFGVN-SHIRDVTERVAKEGYVAIAPAIYQRQAPGFEEGYTPEGIE 85 Query: 312 EFMKWLNE-AGAYSLVEKDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCG 488 K ++ + A L + + IA Q + ++GFC+GG + Sbjct: 86 AGRKLKDQTSSAEILSDLEATIAYAQTLPNVKPEEVGLIGFCFGGWIVYLGASLPTVKAT 145 Query: 489 VSVHGAGISEELVNDIKVPMMF------------------LPAGDDPCLDSVEKLLQSKP 614 S +GAGI + P+ + +P D + +E+ L Sbjct: 146 ASFYGAGIPHWAPGTAEPPITYTDKIQGTLYAFFGLEDTSIPMAD---TEQIEQALTKYQ 202 Query: 615 IGKDCVFHRFDKMTHG-FAAARGDWNNEETRDAVCKVISLAQ 737 + R+ HG F R +N E DA KV L Q Sbjct: 203 VNHK--IFRYPGADHGFFCDQRASYNAEAAADAWQKVKQLFQ 242
>sp|P71505|DLHH_METEX Putative carboxymethylenebutenolidase precursor (Dienelactone hydrolase) (DLH) Length = 291 Score = 38.1 bits (87), Expect = 0.030 Identities = 43/189 (22%), Positives = 84/189 (44%), Gaps = 18/189 (9%) Frame = +3 Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF--RGKPWTLENFPPKNSGEFMKWLN 332 +++V +IFG + + LA+L + + P+++ +G TL + S K + Sbjct: 81 ILVVQEIFGVH-EHIKDVCRRLAKLGYFALAPELYARQGDVSTLTDIQQIVSEVVSKVPD 139 Query: 333 EAGAYSLVEKDLVIAREQLQKTGAV--GKPAILGFCWGGKMS-TCACQTSLYSCGVSVHG 503 + V DL A + TG + I GFCWGG+++ A GV+ +G Sbjct: 140 DQ-----VMSDLDAAVAFAKGTGKADTARLGITGFCWGGRITWLYAAHNPAVKAGVAWYG 194 Query: 504 AGISE----------ELVNDIKVPMMFLPAGDDPCLD--SVEKLLQS-KPIGKDCVFHRF 644 + + ++ D+K P++ L G D + +++++ ++ + GK C F + Sbjct: 195 RLVGDSSALMPKNPVDVAADLKAPVLGLYGGADQGIPVATIDRMKEACRAAGKTCDFVVY 254 Query: 645 DKMTHGFAA 671 + H F A Sbjct: 255 PEAGHAFHA 263
>sp|P27100|TCBE_PSESQ Carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH) Length = 238 Score = 36.2 bits (82), Expect = 0.12 Identities = 50/203 (24%), Positives = 82/203 (40%), Gaps = 12/203 (5%) Frame = +3 Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLENFPPKNS-------GEF 317 V++ +IFG N T A LA F + PD++ +E F P + G F Sbjct: 31 VMVAQEIFGVNPFMTEVLA-WLASEGFVGLCPDLYWRHGPGIE-FDPNDEVQRARALGMF 88 Query: 318 MKWLNEAGAYSLVEKDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVSV 497 + E G L + A Q G V A++G+C GG ++ C V Sbjct: 89 RDYKLEDGVADL-RATVAYAASQPFCDGGV---AVIGYCLGGALAYEVAAEGFAQCCVGY 144 Query: 498 HGAGISEEL--VNDIKVPMMFLPAGDDPCLDSVEKLLQSKPIGKD--CVFHRFDKMTHGF 665 +G G + L +K P MF +D + + + L + + H +D H F Sbjct: 145 YGVGFEKRLERARLVKTPSMFHMGTNDHFVTAEARQLITNAFEANPAIALHWYD-AGHSF 203 Query: 666 A-AARGDWNNEETRDAVCKVISL 731 A A+ +++ E TR A + + + Sbjct: 204 ARASSPNFSPEATRTANARTLEM 226
>sp|P39721|YAE9_YEAST Hypothetical 27.1 kDa protein in ACS1-GCV3 intergenic region Length = 246 Score = 36.2 bits (82), Expect = 0.12 Identities = 58/241 (24%), Positives = 99/241 (41%), Gaps = 10/241 (4%) Frame = +3 Query: 33 EGVKVCCTSGQPQCDKYTSVGTEIDTSKDFKLYQVGSGN--KNGVIIVYDIFGFNIGQTR 206 EGV C G P+ + G +DT Y GS + + ++I+ D++G Sbjct: 12 EGV---CHDGTPKGRREEIFG--LDT------YAAGSTSPKEKVIVILTDVYGNKFNNVL 60 Query: 207 RFADSLAELAFNVVMPDIFRGKPWTLENFPPKNSGEFMKWLNEAGAYSLVEKDLVIAREQ 386 AD A + V +PDI G + + P F + E V K +V + Sbjct: 61 LTADKFASAGYMVFVPDILFGDAISSDK-PIDRDAWFQRHSPE------VTKKIVDGFMK 113 Query: 387 LQKTGAVGK-PAILGFCWGGKMST--CACQTSLYSCGVSVHGAGISEELVN--DIKVPMM 551 L K K ++G+C+G K + + L + H + +S E + D K P++ Sbjct: 114 LLKLEYDPKFIGVVGYCFGAKFAVQHISGDGGLANAAAIAHPSFVSIEEIEAIDSKKPIL 173 Query: 552 FLPAGDDPCLDSVEKLLQSKPIGKDCVFHR---FDKMTHGFAAARGDWNNEETRDAVCKV 722 A +D + + L + + + ++ F + HGF AARGD + + A KV Sbjct: 174 ISAAEEDHIFPANLRHLTEEKLKDNHATYQLDLFSGVAHGF-AARGDISIPAVKYAKEKV 232 Query: 723 I 725 + Sbjct: 233 L 233
>sp|Q8Z3B8|DLHH_SALTI Putative carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH) Length = 270 Score = 35.4 bits (80), Expect = 0.20 Identities = 41/166 (24%), Positives = 71/166 (42%), Gaps = 16/166 (9%) Frame = +3 Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF--RGKPWTLENFPPKNSGEFMKWLN 332 VI+V +IFG + R LA + + P+++ G P + P SG K + Sbjct: 59 VIVVQEIFGVH-EHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPD 117 Query: 333 EAGAYSLVEKDLV-IAREQLQKTGAVGKPAILGFCWGGKMS-TCACQTSLYSCGVSVHGA 506 S V DL +A + G + I GFCWGG+++ A V+ +G Sbjct: 118 -----SQVLADLDHVASWASRNGGDAHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGK 172 Query: 507 GISE----------ELVNDIKVPMMFLPAGDDPCL--DSVEKLLQS 608 + + ++ D+ P++ L +G D + +SVE + Q+ Sbjct: 173 LVGDTSLNSPKHPVDIATDLNAPVLGLYSGQDTSIPQESVETMRQA 218
>sp|P56262|DLHH_ECOLI Putative carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH) Length = 271 Score = 35.4 bits (80), Expect = 0.20 Identities = 43/166 (25%), Positives = 71/166 (42%), Gaps = 16/166 (9%) Frame = +3 Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF--RGKPWTLENFPPKNSGEFMKWLN 332 VI+V +IFG + R LA + + P+++ G P + P SG K + Sbjct: 59 VIVVQEIFGVH-EHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPD 117 Query: 333 EAGAYSLVEKDLV-IAREQLQKTGAVGKPAILGFCWGGKMS-TCACQTSLYSCGVSVHGA 506 S V DL +A + G V + I GFCWGG+++ A V+ +G Sbjct: 118 -----SQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGK 172 Query: 507 GISEELVN----------DIKVPMMFLPAGDDPCL--DSVEKLLQS 608 ++ +N D+ P++ L G D + +SVE + Q+ Sbjct: 173 LTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA 218
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 101,073,520 Number of Sequences: 369166 Number of extensions: 2144663 Number of successful extensions: 4354 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 4221 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4348 length of database: 68,354,980 effective HSP length: 109 effective length of database: 48,218,865 effective search space used: 8148988185 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)