Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_P22
(702 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O14359|YB4E_SCHPO Hypothetical protein C30D10.14 in chro... 66 1e-10
sp|Q9ZT66|E134_MAIZE Endo-1,3;1,4-beta-D-glucanase precursor 56 8e-08
sp|P0A115|CLCD_PSESB Carboxymethylenebutenolidase (Dienelac... 47 4e-05
sp|P27136|TFDE1_RALEJ Carboxymethylenebutenolidase I (Diene... 39 0.010
sp|P73163|DLHH_SYNY3 Putative carboxymethylenebutenolidase ... 39 0.013
sp|P71505|DLHH_METEX Putative carboxymethylenebutenolidase ... 38 0.023
sp|P27100|TCBE_PSESQ Carboxymethylenebutenolidase (Dienelac... 36 0.086
sp|P39721|YAE9_YEAST Hypothetical 27.1 kDa protein in ACS1-... 36 0.11
sp|Q8Z3B8|DLHH_SALTI Putative carboxymethylenebutenolidase ... 35 0.15
sp|P56262|DLHH_ECOLI Putative carboxymethylenebutenolidase ... 35 0.15
>sp|O14359|YB4E_SCHPO Hypothetical protein C30D10.14 in chromosome II
Length = 249
Score = 65.9 bits (159), Expect = 1e-10
Identities = 60/218 (27%), Positives = 92/218 (42%), Gaps = 14/218 (6%)
Frame = +1
Query: 1 VGSGNKNGVIIVY-DIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLENFPPKNSG 177
VGS + V+I + DIFG + Q + AD LA+ F V +PD GKP + PPK
Sbjct: 36 VGSTSNTRVLIGFMDIFGLS-DQIKEGADKLADDGFTVYLPDFLEGKPLPVTALPPKTPE 94
Query: 178 E-------FMKWLNEAGAYSLVEKDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQT 336
+ F ++ + + K + R +G GFCWG K+ T
Sbjct: 95 DQKLCNDFFSTRISPNLHWPKLAKVVEAVRANHGPNVTIG---TYGFCWGAKVLVTYPAT 151
Query: 337 SLYSCGVSVHGAGISEELVNDIKVPMMFLPAGDDPC--LDSVEKLLQSKPIGKDCVFHRF 510
+ S H + ++ P++FL + D+ + E+ ++ P F F
Sbjct: 152 IDFVGIASCHPSFPDSADAANVHCPVLFLCSKDEDAKIIKEWEEAFKTNPAYAKSSFETF 211
Query: 511 DKMTHGFAAARGDWNNEETR----DAVCKVISLAQNFM 612
M HG+ AAR D +N E R + KV S Q+ M
Sbjct: 212 SDMFHGWMAARADLSNPEQRKRFDEGYQKVSSFFQSLM 249
>sp|Q9ZT66|E134_MAIZE Endo-1,3;1,4-beta-D-glucanase precursor
Length = 303
Score = 56.2 bits (134), Expect = 8e-08
Identities = 39/146 (26%), Positives = 68/146 (46%), Gaps = 2/146 (1%)
Frame = +1
Query: 7 SGNKNGVIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLENFPPKNSGEFM 186
+ + V++ D+FG+ R+ D +A+ + VV+PD +G + K + F
Sbjct: 65 ASSSRAVVLASDVFGYEAPLLRQIVDKVAKAGYFVVVPDFLKG-----DYLDDKKN--FT 117
Query: 187 KWLNEAGAYSLVE--KDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVS 360
+WL E K L A ++ K+ AVG G+CWGGK+S +TS
Sbjct: 118 EWLEAHSPVKAAEDAKPLFAALKKEGKSVAVG-----GYCWGGKLSVEVGKTSDVKAVCL 172
Query: 361 VHGAGISEELVNDIKVPMMFLPAGDD 438
H ++ + + ++K P+ L A +D
Sbjct: 173 SHPYSVTADDMKEVKWPIEILGAQND 198
>sp|P0A115|CLCD_PSESB Carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH)
sp|P0A114|CLCD_PSEPU Carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH)
Length = 236
Score = 47.4 bits (111), Expect = 4e-05
Identities = 46/180 (25%), Positives = 79/180 (43%), Gaps = 10/180 (5%)
Frame = +1
Query: 25 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF-RGKPWTLENFPPKNSGE--FMKWL 195
++I +IFG N R L + + V PD++ R P T + + E + W
Sbjct: 31 IVIAQEIFGVNAFM-RETVSWLVDQGYAAVCPDLYARQAPGTALDPQDERQREQAYKLWQ 89
Query: 196 N---EAGAYSLVEKDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVSVH 366
EAG L E + AR Q G VG ++G+C GG ++ V +
Sbjct: 90 AFDMEAGVGDL-EAAIRYARHQPYSNGKVG---LVGYCLGGALAFLVAAKGYVDRAVGYY 145
Query: 367 GAGISEEL--VNDIKVPMMFLPAGDDPCLDSVEKLLQSKPIGKDCVF--HRFDKMTHGFA 534
G G+ ++L V ++K P +F G D + + + L ++ G + + H +++ H FA
Sbjct: 146 GVGLEKQLKKVPEVKHPALFHMGGQDHFVPAPSRQLITEGFGANPLLQVHWYEEAGHSFA 205
>sp|P27136|TFDE1_RALEJ Carboxymethylenebutenolidase I (Dienelactone hydrolase I) (DLH I)
Length = 234
Score = 39.3 bits (90), Expect = 0.010
Identities = 39/141 (27%), Positives = 61/141 (43%), Gaps = 9/141 (6%)
Frame = +1
Query: 25 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLE---NFPPKNSGE---FM 186
V+I +IFG R + L F V PD++ + +E N P + F
Sbjct: 31 VVIAQEIFGIT-PFIRETVEWLVGAGFGCVCPDLYWRQAPNIELDANVPSEREQALALFR 89
Query: 187 KWLNEAGAYSLVEKDLVIAREQLQKTG-AVGKPAILGFCWGGKMSTCACQTSLYSCGVSV 363
+ EAG DL A E + + G+ A++G+C GG ++ SL C +
Sbjct: 90 DFDMEAGV-----NDLSCAIEYARALPFSNGRVAVVGYCLGGALAFDVAARSLADCSIGY 144
Query: 364 HGAGISEE--LVNDIKVPMMF 420
+G G+ ++ LV I P MF
Sbjct: 145 YGVGLEKKVSLVPAITRPAMF 165
>sp|P73163|DLHH_SYNY3 Putative carboxymethylenebutenolidase (Dienelactone hydrolase)
(DLH)
Length = 246
Score = 38.9 bits (89), Expect = 0.013
Identities = 50/222 (22%), Positives = 82/222 (36%), Gaps = 21/222 (9%)
Frame = +1
Query: 1 VGSGNKNGVIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF-RGKPWTLENFPPKNSG 177
V G VI++ +IFG N R + +A+ + + P I+ R P E + P+
Sbjct: 27 VAVGQYPVVIVIQEIFGVN-SHIRDVTERVAKEGYVAIAPAIYQRQAPGFEEGYTPEGIE 85
Query: 178 EFMKWLNE-AGAYSLVEKDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCG 354
K ++ + A L + + IA Q + ++GFC+GG +
Sbjct: 86 AGRKLKDQTSSAEILSDLEATIAYAQTLPNVKPEEVGLIGFCFGGWIVYLGASLPTVKAT 145
Query: 355 VSVHGAGISEELVNDIKVPMMF------------------LPAGDDPCLDSVEKLLQSKP 480
S +GAGI + P+ + +P D + +E+ L
Sbjct: 146 ASFYGAGIPHWAPGTAEPPITYTDKIQGTLYAFFGLEDTSIPMAD---TEQIEQALTKYQ 202
Query: 481 IGKDCVFHRFDKMTHG-FAAARGDWNNEETRDAVCKVISLAQ 603
+ R+ HG F R +N E DA KV L Q
Sbjct: 203 VNHK--IFRYPGADHGFFCDQRASYNAEAAADAWQKVKQLFQ 242
>sp|P71505|DLHH_METEX Putative carboxymethylenebutenolidase precursor (Dienelactone
hydrolase) (DLH)
Length = 291
Score = 38.1 bits (87), Expect = 0.023
Identities = 43/189 (22%), Positives = 84/189 (44%), Gaps = 18/189 (9%)
Frame = +1
Query: 25 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF--RGKPWTLENFPPKNSGEFMKWLN 198
+++V +IFG + + LA+L + + P+++ +G TL + S K +
Sbjct: 81 ILVVQEIFGVH-EHIKDVCRRLAKLGYFALAPELYARQGDVSTLTDIQQIVSEVVSKVPD 139
Query: 199 EAGAYSLVEKDLVIAREQLQKTGAV--GKPAILGFCWGGKMS-TCACQTSLYSCGVSVHG 369
+ V DL A + TG + I GFCWGG+++ A GV+ +G
Sbjct: 140 DQ-----VMSDLDAAVAFAKGTGKADTARLGITGFCWGGRITWLYAAHNPAVKAGVAWYG 194
Query: 370 AGISE----------ELVNDIKVPMMFLPAGDDPCLD--SVEKLLQS-KPIGKDCVFHRF 510
+ + ++ D+K P++ L G D + +++++ ++ + GK C F +
Sbjct: 195 RLVGDSSALMPKNPVDVAADLKAPVLGLYGGADQGIPVATIDRMKEACRAAGKTCDFVVY 254
Query: 511 DKMTHGFAA 537
+ H F A
Sbjct: 255 PEAGHAFHA 263
>sp|P27100|TCBE_PSESQ Carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH)
Length = 238
Score = 36.2 bits (82), Expect = 0.086
Identities = 50/203 (24%), Positives = 82/203 (40%), Gaps = 12/203 (5%)
Frame = +1
Query: 25 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLENFPPKNS-------GEF 183
V++ +IFG N T A LA F + PD++ +E F P + G F
Sbjct: 31 VMVAQEIFGVNPFMTEVLA-WLASEGFVGLCPDLYWRHGPGIE-FDPNDEVQRARALGMF 88
Query: 184 MKWLNEAGAYSLVEKDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVSV 363
+ E G L + A Q G V A++G+C GG ++ C V
Sbjct: 89 RDYKLEDGVADL-RATVAYAASQPFCDGGV---AVIGYCLGGALAYEVAAEGFAQCCVGY 144
Query: 364 HGAGISEEL--VNDIKVPMMFLPAGDDPCLDSVEKLLQSKPIGKD--CVFHRFDKMTHGF 531
+G G + L +K P MF +D + + + L + + H +D H F
Sbjct: 145 YGVGFEKRLERARLVKTPSMFHMGTNDHFVTAEARQLITNAFEANPAIALHWYD-AGHSF 203
Query: 532 A-AARGDWNNEETRDAVCKVISL 597
A A+ +++ E TR A + + +
Sbjct: 204 ARASSPNFSPEATRTANARTLEM 226
>sp|P39721|YAE9_YEAST Hypothetical 27.1 kDa protein in ACS1-GCV3 intergenic region
Length = 246
Score = 35.8 bits (81), Expect = 0.11
Identities = 46/197 (23%), Positives = 82/197 (41%), Gaps = 8/197 (4%)
Frame = +1
Query: 25 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLENFPPKNSGEFMKWLNEA 204
++I+ D++G AD A + V +PDI G + + P F + E
Sbjct: 45 IVILTDVYGNKFNNVLLTADKFASAGYMVFVPDILFGDAISSDK-PIDRDAWFQRHSPE- 102
Query: 205 GAYSLVEKDLVIAREQLQKTGAVGK-PAILGFCWGGKMST--CACQTSLYSCGVSVHGAG 375
V K +V +L K K ++G+C+G K + + L + H +
Sbjct: 103 -----VTKKIVDGFMKLLKLEYDPKFIGVVGYCFGAKFAVQHISGDGGLANAAAIAHPSF 157
Query: 376 ISEELVN--DIKVPMMFLPAGDDPCLDSVEKLLQSKPIGKDCVFHR---FDKMTHGFAAA 540
+S E + D K P++ A +D + + L + + + ++ F + HGF AA
Sbjct: 158 VSIEEIEAIDSKKPILISAAEEDHIFPANLRHLTEEKLKDNHATYQLDLFSGVAHGF-AA 216
Query: 541 RGDWNNEETRDAVCKVI 591
RGD + + A KV+
Sbjct: 217 RGDISIPAVKYAKEKVL 233
>sp|Q8Z3B8|DLHH_SALTI Putative carboxymethylenebutenolidase (Dienelactone hydrolase)
(DLH)
Length = 270
Score = 35.4 bits (80), Expect = 0.15
Identities = 41/166 (24%), Positives = 71/166 (42%), Gaps = 16/166 (9%)
Frame = +1
Query: 25 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF--RGKPWTLENFPPKNSGEFMKWLN 198
VI+V +IFG + R LA + + P+++ G P + P SG K +
Sbjct: 59 VIVVQEIFGVH-EHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPD 117
Query: 199 EAGAYSLVEKDLV-IAREQLQKTGAVGKPAILGFCWGGKMS-TCACQTSLYSCGVSVHGA 372
S V DL +A + G + I GFCWGG+++ A V+ +G
Sbjct: 118 -----SQVLADLDHVASWASRNGGDAHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGK 172
Query: 373 GISE----------ELVNDIKVPMMFLPAGDDPCL--DSVEKLLQS 474
+ + ++ D+ P++ L +G D + +SVE + Q+
Sbjct: 173 LVGDTSLNSPKHPVDIATDLNAPVLGLYSGQDTSIPQESVETMRQA 218
>sp|P56262|DLHH_ECOLI Putative carboxymethylenebutenolidase (Dienelactone hydrolase)
(DLH)
Length = 271
Score = 35.4 bits (80), Expect = 0.15
Identities = 43/166 (25%), Positives = 71/166 (42%), Gaps = 16/166 (9%)
Frame = +1
Query: 25 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF--RGKPWTLENFPPKNSGEFMKWLN 198
VI+V +IFG + R LA + + P+++ G P + P SG K +
Sbjct: 59 VIVVQEIFGVH-EHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPD 117
Query: 199 EAGAYSLVEKDLV-IAREQLQKTGAVGKPAILGFCWGGKMS-TCACQTSLYSCGVSVHGA 372
S V DL +A + G V + I GFCWGG+++ A V+ +G
Sbjct: 118 -----SQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGK 172
Query: 373 GISEELVN----------DIKVPMMFLPAGDDPCL--DSVEKLLQS 474
++ +N D+ P++ L G D + +SVE + Q+
Sbjct: 173 LTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA 218
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,163,956
Number of Sequences: 369166
Number of extensions: 1749940
Number of successful extensions: 3588
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3506
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3583
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6122130210
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)