Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_J09
(833 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O14359|YB4E_SCHPO Hypothetical protein C30D10.14 in chro... 75 3e-13
sp|Q9ZT66|E134_MAIZE Endo-1,3;1,4-beta-D-glucanase precursor 56 1e-07
sp|P0A115|CLCD_PSESB Carboxymethylenebutenolidase (Dienelac... 47 5e-05
sp|P27136|TFDE1_RALEJ Carboxymethylenebutenolidase I (Diene... 39 0.014
sp|P73163|DLHH_SYNY3 Putative carboxymethylenebutenolidase ... 39 0.018
sp|P71505|DLHH_METEX Putative carboxymethylenebutenolidase ... 38 0.030
sp|P27100|TCBE_PSESQ Carboxymethylenebutenolidase (Dienelac... 36 0.11
sp|P39721|YAE9_YEAST Hypothetical 27.1 kDa protein in ACS1-... 36 0.11
sp|Q8Z3B8|DLHH_SALTI Putative carboxymethylenebutenolidase ... 35 0.20
sp|P56262|DLHH_ECOLI Putative carboxymethylenebutenolidase ... 35 0.20
>sp|O14359|YB4E_SCHPO Hypothetical protein C30D10.14 in chromosome II
Length = 249
Score = 74.7 bits (182), Expect = 3e-13
Identities = 68/250 (27%), Positives = 103/250 (41%), Gaps = 17/250 (6%)
Frame = +3
Query: 48 CCTSGQPQC---DKYTSVGTEIDTSKDFKLYQVGSGNKNGVIIVY-DIFGFNIGQTRRFA 215
CC + + YT G EI+ Y VGS + V+I + DIFG + Q + A
Sbjct: 4 CCPTSRGAAASNKSYTFKGKEIENFGGLTTYVVGSTSNTRVLIGFMDIFGLS-DQIKEGA 62
Query: 216 DSLAELAFNVVMPDIFRGKPWTLENFPPKNSGE-------FMKWLNEAGAYSLVEKDLVI 374
D LA+ F V +PD GKP + PPK + F ++ + + K +
Sbjct: 63 DKLADDGFTVYLPDFLEGKPLPVTALPPKTPEDQKLCNDFFSTRISPNLHWPKLAKVVEA 122
Query: 375 AREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVSVHGAGISEELVNDIKVPMMF 554
R +G GFCWG K+ T + S H + ++ P++F
Sbjct: 123 VRANHGPNVTIG---TYGFCWGAKVLVTYPATIDFVGIASCHPSFPDSADAANVHCPVLF 179
Query: 555 LPAGDDPC--LDSVEKLLQSKPIGKDCVFHRFDKMTHGFAAARGDWNNEETR----DAVC 716
L + D+ + E+ ++ P F F M HG+ AAR D +N E R +
Sbjct: 180 LCSKDEDAKIIKEWEEAFKTNPAYAKSSFETFSDMFHGWMAARADLSNPEQRKRFDEGYQ 239
Query: 717 KVISLAQNFM 746
KV S Q+ M
Sbjct: 240 KVSSFFQSLM 249
>sp|Q9ZT66|E134_MAIZE Endo-1,3;1,4-beta-D-glucanase precursor
Length = 303
Score = 56.2 bits (134), Expect = 1e-07
Identities = 39/146 (26%), Positives = 68/146 (46%), Gaps = 2/146 (1%)
Frame = +3
Query: 141 SGNKNGVIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLENFPPKNSGEFM 320
+ + V++ D+FG+ R+ D +A+ + VV+PD +G + K + F
Sbjct: 65 ASSSRAVVLASDVFGYEAPLLRQIVDKVAKAGYFVVVPDFLKG-----DYLDDKKN--FT 117
Query: 321 KWLNEAGAYSLVE--KDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVS 494
+WL E K L A ++ K+ AVG G+CWGGK+S +TS
Sbjct: 118 EWLEAHSPVKAAEDAKPLFAALKKEGKSVAVG-----GYCWGGKLSVEVGKTSDVKAVCL 172
Query: 495 VHGAGISEELVNDIKVPMMFLPAGDD 572
H ++ + + ++K P+ L A +D
Sbjct: 173 SHPYSVTADDMKEVKWPIEILGAQND 198
>sp|P0A115|CLCD_PSESB Carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH)
sp|P0A114|CLCD_PSEPU Carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH)
Length = 236
Score = 47.4 bits (111), Expect = 5e-05
Identities = 46/180 (25%), Positives = 79/180 (43%), Gaps = 10/180 (5%)
Frame = +3
Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF-RGKPWTLENFPPKNSGE--FMKWL 329
++I +IFG N R L + + V PD++ R P T + + E + W
Sbjct: 31 IVIAQEIFGVNAFM-RETVSWLVDQGYAAVCPDLYARQAPGTALDPQDERQREQAYKLWQ 89
Query: 330 N---EAGAYSLVEKDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVSVH 500
EAG L E + AR Q G VG ++G+C GG ++ V +
Sbjct: 90 AFDMEAGVGDL-EAAIRYARHQPYSNGKVG---LVGYCLGGALAFLVAAKGYVDRAVGYY 145
Query: 501 GAGISEEL--VNDIKVPMMFLPAGDDPCLDSVEKLLQSKPIGKDCVF--HRFDKMTHGFA 668
G G+ ++L V ++K P +F G D + + + L ++ G + + H +++ H FA
Sbjct: 146 GVGLEKQLKKVPEVKHPALFHMGGQDHFVPAPSRQLITEGFGANPLLQVHWYEEAGHSFA 205
>sp|P27136|TFDE1_RALEJ Carboxymethylenebutenolidase I (Dienelactone hydrolase I) (DLH I)
Length = 234
Score = 39.3 bits (90), Expect = 0.014
Identities = 39/141 (27%), Positives = 61/141 (43%), Gaps = 9/141 (6%)
Frame = +3
Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLE---NFPPKNSGE---FM 320
V+I +IFG R + L F V PD++ + +E N P + F
Sbjct: 31 VVIAQEIFGIT-PFIRETVEWLVGAGFGCVCPDLYWRQAPNIELDANVPSEREQALALFR 89
Query: 321 KWLNEAGAYSLVEKDLVIAREQLQKTG-AVGKPAILGFCWGGKMSTCACQTSLYSCGVSV 497
+ EAG DL A E + + G+ A++G+C GG ++ SL C +
Sbjct: 90 DFDMEAGV-----NDLSCAIEYARALPFSNGRVAVVGYCLGGALAFDVAARSLADCSIGY 144
Query: 498 HGAGISEE--LVNDIKVPMMF 554
+G G+ ++ LV I P MF
Sbjct: 145 YGVGLEKKVSLVPAITRPAMF 165
>sp|P73163|DLHH_SYNY3 Putative carboxymethylenebutenolidase (Dienelactone hydrolase)
(DLH)
Length = 246
Score = 38.9 bits (89), Expect = 0.018
Identities = 50/222 (22%), Positives = 82/222 (36%), Gaps = 21/222 (9%)
Frame = +3
Query: 135 VGSGNKNGVIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF-RGKPWTLENFPPKNSG 311
V G VI++ +IFG N R + +A+ + + P I+ R P E + P+
Sbjct: 27 VAVGQYPVVIVIQEIFGVN-SHIRDVTERVAKEGYVAIAPAIYQRQAPGFEEGYTPEGIE 85
Query: 312 EFMKWLNE-AGAYSLVEKDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCG 488
K ++ + A L + + IA Q + ++GFC+GG +
Sbjct: 86 AGRKLKDQTSSAEILSDLEATIAYAQTLPNVKPEEVGLIGFCFGGWIVYLGASLPTVKAT 145
Query: 489 VSVHGAGISEELVNDIKVPMMF------------------LPAGDDPCLDSVEKLLQSKP 614
S +GAGI + P+ + +P D + +E+ L
Sbjct: 146 ASFYGAGIPHWAPGTAEPPITYTDKIQGTLYAFFGLEDTSIPMAD---TEQIEQALTKYQ 202
Query: 615 IGKDCVFHRFDKMTHG-FAAARGDWNNEETRDAVCKVISLAQ 737
+ R+ HG F R +N E DA KV L Q
Sbjct: 203 VNHK--IFRYPGADHGFFCDQRASYNAEAAADAWQKVKQLFQ 242
>sp|P71505|DLHH_METEX Putative carboxymethylenebutenolidase precursor (Dienelactone
hydrolase) (DLH)
Length = 291
Score = 38.1 bits (87), Expect = 0.030
Identities = 43/189 (22%), Positives = 84/189 (44%), Gaps = 18/189 (9%)
Frame = +3
Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF--RGKPWTLENFPPKNSGEFMKWLN 332
+++V +IFG + + LA+L + + P+++ +G TL + S K +
Sbjct: 81 ILVVQEIFGVH-EHIKDVCRRLAKLGYFALAPELYARQGDVSTLTDIQQIVSEVVSKVPD 139
Query: 333 EAGAYSLVEKDLVIAREQLQKTGAV--GKPAILGFCWGGKMS-TCACQTSLYSCGVSVHG 503
+ V DL A + TG + I GFCWGG+++ A GV+ +G
Sbjct: 140 DQ-----VMSDLDAAVAFAKGTGKADTARLGITGFCWGGRITWLYAAHNPAVKAGVAWYG 194
Query: 504 AGISE----------ELVNDIKVPMMFLPAGDDPCLD--SVEKLLQS-KPIGKDCVFHRF 644
+ + ++ D+K P++ L G D + +++++ ++ + GK C F +
Sbjct: 195 RLVGDSSALMPKNPVDVAADLKAPVLGLYGGADQGIPVATIDRMKEACRAAGKTCDFVVY 254
Query: 645 DKMTHGFAA 671
+ H F A
Sbjct: 255 PEAGHAFHA 263
>sp|P27100|TCBE_PSESQ Carboxymethylenebutenolidase (Dienelactone hydrolase) (DLH)
Length = 238
Score = 36.2 bits (82), Expect = 0.11
Identities = 50/203 (24%), Positives = 82/203 (40%), Gaps = 12/203 (5%)
Frame = +3
Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIFRGKPWTLENFPPKNS-------GEF 317
V++ +IFG N T A LA F + PD++ +E F P + G F
Sbjct: 31 VMVAQEIFGVNPFMTEVLA-WLASEGFVGLCPDLYWRHGPGIE-FDPNDEVQRARALGMF 88
Query: 318 MKWLNEAGAYSLVEKDLVIAREQLQKTGAVGKPAILGFCWGGKMSTCACQTSLYSCGVSV 497
+ E G L + A Q G V A++G+C GG ++ C V
Sbjct: 89 RDYKLEDGVADL-RATVAYAASQPFCDGGV---AVIGYCLGGALAYEVAAEGFAQCCVGY 144
Query: 498 HGAGISEEL--VNDIKVPMMFLPAGDDPCLDSVEKLLQSKPIGKD--CVFHRFDKMTHGF 665
+G G + L +K P MF +D + + + L + + H +D H F
Sbjct: 145 YGVGFEKRLERARLVKTPSMFHMGTNDHFVTAEARQLITNAFEANPAIALHWYD-AGHSF 203
Query: 666 A-AARGDWNNEETRDAVCKVISL 731
A A+ +++ E TR A + + +
Sbjct: 204 ARASSPNFSPEATRTANARTLEM 226
>sp|P39721|YAE9_YEAST Hypothetical 27.1 kDa protein in ACS1-GCV3 intergenic region
Length = 246
Score = 36.2 bits (82), Expect = 0.11
Identities = 58/241 (24%), Positives = 99/241 (41%), Gaps = 10/241 (4%)
Frame = +3
Query: 33 EGVKVCCTSGQPQCDKYTSVGTEIDTSKDFKLYQVGSGN--KNGVIIVYDIFGFNIGQTR 206
EGV C G P+ + G +DT Y GS + + ++I+ D++G
Sbjct: 12 EGV---CHDGTPKGRREEIFG--LDT------YAAGSTSPKEKVIVILTDVYGNKFNNVL 60
Query: 207 RFADSLAELAFNVVMPDIFRGKPWTLENFPPKNSGEFMKWLNEAGAYSLVEKDLVIAREQ 386
AD A + V +PDI G + + P F + E V K +V +
Sbjct: 61 LTADKFASAGYMVFVPDILFGDAISSDK-PIDRDAWFQRHSPE------VTKKIVDGFMK 113
Query: 387 LQKTGAVGK-PAILGFCWGGKMST--CACQTSLYSCGVSVHGAGISEELVN--DIKVPMM 551
L K K ++G+C+G K + + L + H + +S E + D K P++
Sbjct: 114 LLKLEYDPKFIGVVGYCFGAKFAVQHISGDGGLANAAAIAHPSFVSIEEIEAIDSKKPIL 173
Query: 552 FLPAGDDPCLDSVEKLLQSKPIGKDCVFHR---FDKMTHGFAAARGDWNNEETRDAVCKV 722
A +D + + L + + + ++ F + HGF AARGD + + A KV
Sbjct: 174 ISAAEEDHIFPANLRHLTEEKLKDNHATYQLDLFSGVAHGF-AARGDISIPAVKYAKEKV 232
Query: 723 I 725
+
Sbjct: 233 L 233
>sp|Q8Z3B8|DLHH_SALTI Putative carboxymethylenebutenolidase (Dienelactone hydrolase)
(DLH)
Length = 270
Score = 35.4 bits (80), Expect = 0.20
Identities = 41/166 (24%), Positives = 71/166 (42%), Gaps = 16/166 (9%)
Frame = +3
Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF--RGKPWTLENFPPKNSGEFMKWLN 332
VI+V +IFG + R LA + + P+++ G P + P SG K +
Sbjct: 59 VIVVQEIFGVH-EHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPD 117
Query: 333 EAGAYSLVEKDLV-IAREQLQKTGAVGKPAILGFCWGGKMS-TCACQTSLYSCGVSVHGA 506
S V DL +A + G + I GFCWGG+++ A V+ +G
Sbjct: 118 -----SQVLADLDHVASWASRNGGDAHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGK 172
Query: 507 GISE----------ELVNDIKVPMMFLPAGDDPCL--DSVEKLLQS 608
+ + ++ D+ P++ L +G D + +SVE + Q+
Sbjct: 173 LVGDTSLNSPKHPVDIATDLNAPVLGLYSGQDTSIPQESVETMRQA 218
>sp|P56262|DLHH_ECOLI Putative carboxymethylenebutenolidase (Dienelactone hydrolase)
(DLH)
Length = 271
Score = 35.4 bits (80), Expect = 0.20
Identities = 43/166 (25%), Positives = 71/166 (42%), Gaps = 16/166 (9%)
Frame = +3
Query: 159 VIIVYDIFGFNIGQTRRFADSLAELAFNVVMPDIF--RGKPWTLENFPPKNSGEFMKWLN 332
VI+V +IFG + R LA + + P+++ G P + P SG K +
Sbjct: 59 VIVVQEIFGVH-EHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPD 117
Query: 333 EAGAYSLVEKDLV-IAREQLQKTGAVGKPAILGFCWGGKMS-TCACQTSLYSCGVSVHGA 506
S V DL +A + G V + I GFCWGG+++ A V+ +G
Sbjct: 118 -----SQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGK 172
Query: 507 GISEELVN----------DIKVPMMFLPAGDDPCL--DSVEKLLQS 608
++ +N D+ P++ L G D + +SVE + Q+
Sbjct: 173 LTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA 218
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 98,892,074
Number of Sequences: 369166
Number of extensions: 2084437
Number of successful extensions: 4269
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4263
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8100769320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)