Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= DrC_00973 (712 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|Q61496|DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh) 102 1e-21 sp|P09052|VASA_DROME Vasa protein (Antigen Mab46F11) 98 2e-20 sp|Q64060|DDX4_RAT DEAD-box protein 4 (VASA homolog) (rVLG) 97 4e-20 sp|P24784|DBP1_YEAST Probable ATP-dependent RNA helicase DB... 96 7e-20 sp|P16381|PL10_MOUSE Putative ATP-dependent RNA helicase PL10 96 1e-19 sp|P24346|AN3_XENLA Putative ATP-dependent RNA helicase An3 95 2e-19 sp|Q62095|DDX3Y_MOUSE DEAD-box protein 3, Y-chromosomal (DE... 95 2e-19 sp|Q62167|DDX3X_MOUSE DEAD-box protein 3, X-chromosomal (DE... 95 2e-19 sp|O00571|DDX3X_HUMAN DEAD-box protein 3, X-chromosomal (He... 94 3e-19 sp|Q9NQI0|DDX4_HUMAN DEAD-box protein 4 (VASA homolog) 94 4e-19
>sp|Q61496|DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh) Length = 702 Score = 102 bits (253), Expect = 1e-21 Identities = 53/137 (38%), Positives = 81/137 (59%), Gaps = 3/137 (2%) Frame = +3 Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185 +HGD QR+R+ G F+ C VLVATSVAARG+D+ +++ V+N PS +EY+HR G Sbjct: 543 IHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIG 602 Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSS- 362 R GR G G A++F +D +N + +VK+L+ ++VP ++ EI + + SS Sbjct: 603 RTGRCGNTGRAISFF-DTDSDNHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPPSFSSST 661 Query: 363 --GTRYAKDRLESNYRG 407 G +A NY+G Sbjct: 662 RGGAVFASVDTRKNYQG 678
>sp|P09052|VASA_DROME Vasa protein (Antigen Mab46F11) Length = 661 Score = 97.8 bits (242), Expect = 2e-20 Identities = 47/103 (45%), Positives = 69/103 (66%) Frame = +3 Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185 +HGD Q QR+ FKN + KVL+ATSVA+RG+D+ +I+ V+N PS ++Y+HR G Sbjct: 519 IHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIG 578 Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFM 314 R GR+G G A +F P + + A D+VK+L +G+ VPDF+ Sbjct: 579 RTGRVGNNGRATSFFDP-EKDRAIAADLVKILEGSGQTVPDFL 620
>sp|Q64060|DDX4_RAT DEAD-box protein 4 (VASA homolog) (rVLG) Length = 713 Score = 97.1 bits (240), Expect = 4e-20 Identities = 50/136 (36%), Positives = 79/136 (58%), Gaps = 2/136 (1%) Frame = +3 Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185 +HGD QR+R+ G F+ C VLVATSVAARG+D+ +++ V+N PS +EY+HR G Sbjct: 555 IHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFNLPSTIDEYVHRIG 614 Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEIC--KDVGIQFKYKS 359 R GR G G A++F ++ +N + +VK+L+ ++VP ++ EI F + Sbjct: 615 RTGRCGNTGRAISFF-DTESDNHLAQPLVKVLSDAQQDVPAWLEEIAFSSYAPPSFSNST 673 Query: 360 SGTRYAKDRLESNYRG 407 G +A N++G Sbjct: 674 RGAVFASFDTRKNFQG 689
>sp|P24784|DBP1_YEAST Probable ATP-dependent RNA helicase DBP1 (Helicase CA1) Length = 617 Score = 96.3 bits (238), Expect = 7e-20 Identities = 50/146 (34%), Positives = 81/146 (55%) Frame = +3 Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185 +HGD +Q +R+ FK N +LVAT+VAARG+D+ ++ V+N PS+ ++Y+HR G Sbjct: 441 IHGDRTQAERERALSAFKANVADILVATAVAARGLDIPNVTHVINYDLPSDIDDYVHRIG 500 Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365 R GR G G+A +F + N V+ ++++LN+ +EVP F++++ + Sbjct: 501 RTGRAGNTGVATSFFNSN--NQNIVKGLMEILNEANQEVPTFLSDLSR------------ 546 Query: 366 TRYAKDRLESNYRGGQARYGGDRFES 443 N RGG+ R GG F S Sbjct: 547 ---------QNSRGGRTRGGGGFFNS 563
>sp|P16381|PL10_MOUSE Putative ATP-dependent RNA helicase PL10 Length = 660 Score = 95.5 bits (236), Expect = 1e-19 Identities = 54/176 (30%), Positives = 88/176 (50%) Frame = +3 Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185 +HGD SQR R+ F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR G Sbjct: 470 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 529 Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365 R GR+G G+A +F + N +D++ +L + +EVP ++ +++ + YK Sbjct: 530 RTGRVGNLGLATSFFNERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAFEHHYKGGS 583 Query: 366 TRYAKDRLESNYRGGQARYGGDRFESNXXXXXXXXXXXXXFESNYRDGQARYGGDR 533 +K R + R S+ SN R G +G R Sbjct: 584 RGRSKSRFSGGFGARDYRQSSGASSSSFSSGR---------ASNSRSGGGSHGSSR 630
>sp|P24346|AN3_XENLA Putative ATP-dependent RNA helicase An3 Length = 697 Score = 95.1 bits (235), Expect = 2e-19 Identities = 47/132 (35%), Positives = 80/132 (60%) Frame = +3 Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185 +HGD SQR R+ F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR G Sbjct: 512 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 571 Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365 R GR+G G+A +F + N +D++ +L + +EVP ++ +++ + +KSS Sbjct: 572 RTGRVGNLGLATSFFNEKNIN--ITKDLLDLLVEAKQEVPSWL----ENMAYEQHHKSSS 625 Query: 366 TRYAKDRLESNY 401 +K R + Sbjct: 626 RGRSKSRFSGGF 637
>sp|Q62095|DDX3Y_MOUSE DEAD-box protein 3, Y-chromosomal (DEAD-box RNA helicase DEAD2) (mDEAD2) (D1Pas1-related sequence 1) Length = 658 Score = 95.1 bits (235), Expect = 2e-19 Identities = 55/191 (28%), Positives = 89/191 (46%) Frame = +3 Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185 +HGD SQ+ R+ F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR G Sbjct: 470 IHGDRSQKDREEALHQFRSGRKPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 529 Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365 R GR+G G+A +F + N +D++ +L + +EVP ++ + + + YK S Sbjct: 530 RTGRVGNLGLATSFFNERNLN--ITKDLLDLLVEAKQEVPSWL----ESMAYEHHYKGSS 583 Query: 366 TRYAKDRLESNYRGGQARYGGDRFESNXXXXXXXXXXXXXFESNYRDGQARYGGDRLESN 545 +K R + R + N G YGG Sbjct: 584 RGRSKSRFSGGFGARDYRQSSGSANAGFNSNRANSSRSSGSSHNRGFGGGGYGGFYNNDG 643 Query: 546 LGSDQTKDAED 578 G + A D Sbjct: 644 YGGNYNSQAVD 654
>sp|Q62167|DDX3X_MOUSE DEAD-box protein 3, X-chromosomal (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1Pas1-related sequence 2) Length = 662 Score = 94.7 bits (234), Expect = 2e-19 Identities = 49/135 (36%), Positives = 81/135 (60%) Frame = +3 Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185 +HGD SQR R+ F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR G Sbjct: 471 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 530 Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365 R GR+G G+A +F + N +D++ +L + +EVP ++ +++ + YK S Sbjct: 531 RTGRVGNLGLATSFFNERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAFEHHYKGSS 584 Query: 366 TRYAKDRLESNYRGG 410 +K S + GG Sbjct: 585 RGRSK---SSRFSGG 596
>sp|O00571|DDX3X_HUMAN DEAD-box protein 3, X-chromosomal (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform) Length = 662 Score = 94.4 bits (233), Expect = 3e-19 Identities = 49/135 (36%), Positives = 81/135 (60%) Frame = +3 Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185 +HGD SQR R+ F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR G Sbjct: 471 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 530 Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365 R GR+G G+A +F + N +D++ +L + +EVP ++ +++ + YK S Sbjct: 531 RTGRVGNLGLATSFFNERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAYEHHYKGSS 584 Query: 366 TRYAKDRLESNYRGG 410 +K S + GG Sbjct: 585 RGRSK---SSRFSGG 596
>sp|Q9NQI0|DDX4_HUMAN DEAD-box protein 4 (VASA homolog) Length = 724 Score = 94.0 bits (232), Expect = 4e-19 Identities = 45/106 (42%), Positives = 68/106 (64%) Frame = +3 Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185 +HGD QR+R+ G F+ C VLVATSVAARG+D+ +++ V+N PS +EY+HR G Sbjct: 570 IHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIG 629 Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEI 323 R GR G G A++F + +N + +VK+L ++VP ++ EI Sbjct: 630 RTGRCGNTGRAISFF-DLESDNHLAQPLVKVLTDAQQDVPAWLEEI 674
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 75,963,052 Number of Sequences: 369166 Number of extensions: 1529026 Number of successful extensions: 4846 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 4597 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4804 length of database: 68,354,980 effective HSP length: 107 effective length of database: 48,588,335 effective search space used: 6267895215 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)