Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_E09
(674 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q61496|DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh) 88 2e-17
sp|P09052|VASA_DROME Vasa protein (Antigen Mab46F11) 88 2e-17
sp|P24784|DBP1_YEAST Probable ATP-dependent RNA helicase DB... 85 2e-16
sp|Q64060|DDX4_RAT DEAD-box protein 4 (VASA homolog) (rVLG) 83 6e-16
sp|P16381|PL10_MOUSE Putative ATP-dependent RNA helicase PL10 82 1e-15
sp|P24346|AN3_XENLA Putative ATP-dependent RNA helicase An3 82 1e-15
sp|Q62095|DDX3Y_MOUSE DEAD-box protein 3, Y-chromosomal (DE... 82 2e-15
sp|Q62167|DDX3X_MOUSE DEAD-box protein 3, X-chromosomal (DE... 82 2e-15
sp|Q6BY27|DBP2_DEBHA Probable ATP-dependent RNA helicase DBP2 82 2e-15
sp|O00571|DDX3X_HUMAN DEAD-box protein 3, X-chromosomal (He... 81 2e-15
>sp|Q61496|DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh)
Length = 702
Score = 88.2 bits (217), Expect = 2e-17
Identities = 47/123 (38%), Positives = 72/123 (58%), Gaps = 3/123 (2%)
Frame = +1
Query: 10 GLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTGRCGRMGRQGIAVTF 189
G F+ C VLVATSVAARG+D+ +++ V+N PS +EY+HR GR GR G G A++F
Sbjct: 557 GDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISF 616
Query: 190 IKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSS---GTRYAKDRLESN 360
+D +N + +VK+L+ ++VP ++ EI + + SS G +A N
Sbjct: 617 F-DTDSDNHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPPSFSSSTRGGAVFASVDTRKN 675
Query: 361 YRG 369
Y+G
Sbjct: 676 YQG 678
>sp|P09052|VASA_DROME Vasa protein (Antigen Mab46F11)
Length = 661
Score = 88.2 bits (217), Expect = 2e-17
Identities = 41/87 (47%), Positives = 61/87 (70%)
Frame = +1
Query: 16 FKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTGRCGRMGRQGIAVTFIK 195
FKN + KVL+ATSVA+RG+D+ +I+ V+N PS ++Y+HR GR GR+G G A +F
Sbjct: 535 FKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSFFD 594
Query: 196 PSDFNNACVRDIVKMLNKTGKEVPDFM 276
P + + A D+VK+L +G+ VPDF+
Sbjct: 595 P-EKDRAIAADLVKILEGSGQTVPDFL 620
>sp|P24784|DBP1_YEAST Probable ATP-dependent RNA helicase DBP1 (Helicase CA1)
Length = 617
Score = 85.1 bits (209), Expect = 2e-16
Identities = 45/130 (34%), Positives = 72/130 (55%)
Frame = +1
Query: 16 FKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTGRCGRMGRQGIAVTFIK 195
FK N +LVAT+VAARG+D+ ++ V+N PS+ ++Y+HR GR GR G G+A +F
Sbjct: 457 FKANVADILVATAVAARGLDIPNVTHVINYDLPSDIDDYVHRIGRTGRAGNTGVATSFFN 516
Query: 196 PSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSGTRYAKDRLESNYRGGQ 375
+ N V+ ++++LN+ +EVP F++++ + N RGG+
Sbjct: 517 SN--NQNIVKGLMEILNEANQEVPTFLSDLSR---------------------QNSRGGR 553
Query: 376 ARYGGDRFES 405
R GG F S
Sbjct: 554 TRGGGGFFNS 563
>sp|Q64060|DDX4_RAT DEAD-box protein 4 (VASA homolog) (rVLG)
Length = 713
Score = 83.2 bits (204), Expect = 6e-16
Identities = 44/122 (36%), Positives = 70/122 (57%), Gaps = 2/122 (1%)
Frame = +1
Query: 10 GLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTGRCGRMGRQGIAVTF 189
G F+ C VLVATSVAARG+D+ +++ V+N PS +EY+HR GR GR G G A++F
Sbjct: 569 GDFRCGKCPVLVATSVAARGLDIENVQHVINFNLPSTIDEYVHRIGRTGRCGNTGRAISF 628
Query: 190 IKPSDFNNACVRDIVKMLNKTGKEVPDFMNEIC--KDVGIQFKYKSSGTRYAKDRLESNY 363
++ +N + +VK+L+ ++VP ++ EI F + G +A N+
Sbjct: 629 F-DTESDNHLAQPLVKVLSDAQQDVPAWLEEIAFSSYAPPSFSNSTRGAVFASFDTRKNF 687
Query: 364 RG 369
+G
Sbjct: 688 QG 689
>sp|P16381|PL10_MOUSE Putative ATP-dependent RNA helicase PL10
Length = 660
Score = 82.4 bits (202), Expect = 1e-15
Identities = 47/160 (29%), Positives = 79/160 (49%)
Frame = +1
Query: 16 FKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTGRCGRMGRQGIAVTFIK 195
F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR GR GR+G G+A +F
Sbjct: 486 FRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN 545
Query: 196 PSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSGTRYAKDRLESNYRGGQ 375
+ N +D++ +L + +EVP ++ +++ + YK +K R +
Sbjct: 546 ERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAFEHHYKGGSRGRSKSRFSGGFGARD 599
Query: 376 ARYGGDRFESNXXXXXXXXXXXXXFESNYRDGQARYGGDR 495
R S+ SN R G +G R
Sbjct: 600 YRQSSGASSSSFSSGR---------ASNSRSGGGSHGSSR 630
>sp|P24346|AN3_XENLA Putative ATP-dependent RNA helicase An3
Length = 697
Score = 82.0 bits (201), Expect = 1e-15
Identities = 40/116 (34%), Positives = 71/116 (61%)
Frame = +1
Query: 16 FKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTGRCGRMGRQGIAVTFIK 195
F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR GR GR+G G+A +F
Sbjct: 528 FRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN 587
Query: 196 PSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSGTRYAKDRLESNY 363
+ N +D++ +L + +EVP ++ +++ + +KSS +K R +
Sbjct: 588 EKNIN--ITKDLLDLLVEAKQEVPSWL----ENMAYEQHHKSSSRGRSKSRFSGGF 637
>sp|Q62095|DDX3Y_MOUSE DEAD-box protein 3, Y-chromosomal (DEAD-box RNA helicase DEAD2)
(mDEAD2) (D1Pas1-related sequence 1)
Length = 658
Score = 81.6 bits (200), Expect = 2e-15
Identities = 40/116 (34%), Positives = 69/116 (59%)
Frame = +1
Query: 16 FKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTGRCGRMGRQGIAVTFIK 195
F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR GR GR+G G+A +F
Sbjct: 486 FRSGRKPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN 545
Query: 196 PSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSGTRYAKDRLESNY 363
+ N +D++ +L + +EVP ++ + + + YK S +K R +
Sbjct: 546 ERNLN--ITKDLLDLLVEAKQEVPSWL----ESMAYEHHYKGSSRGRSKSRFSGGF 595
>sp|Q62167|DDX3X_MOUSE DEAD-box protein 3, X-chromosomal (DEAD-box RNA helicase DEAD3)
(mDEAD3) (Embryonic RNA helicase) (D1Pas1-related
sequence 2)
Length = 662
Score = 81.6 bits (200), Expect = 2e-15
Identities = 42/119 (35%), Positives = 72/119 (60%)
Frame = +1
Query: 16 FKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTGRCGRMGRQGIAVTFIK 195
F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR GR GR+G G+A +F
Sbjct: 487 FRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN 546
Query: 196 PSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSGTRYAKDRLESNYRGG 372
+ N +D++ +L + +EVP ++ +++ + YK S +K S + GG
Sbjct: 547 ERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAFEHHYKGSSRGRSK---SSRFSGG 596
>sp|Q6BY27|DBP2_DEBHA Probable ATP-dependent RNA helicase DBP2
Length = 536
Score = 81.6 bits (200), Expect = 2e-15
Identities = 46/126 (36%), Positives = 67/126 (53%), Gaps = 3/126 (2%)
Frame = +1
Query: 16 FKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTGRCGRMGRQGIAVTFIK 195
FK ++VAT VAARGIDV I V+N+ P N E+Y+HR GR GR G G AV+F
Sbjct: 398 FKTGKSPIMVATDVAARGIDVKGISYVINLDMPGNIEDYVHRIGRTGRAGSTGTAVSFF- 456
Query: 196 PSDFNNACVRDIVKMLNKTGKEV-PDFMNEICKDVGIQFKYKSSGTR--YAKDRLESNYR 366
+D N+ D+ K++ + + + P+ M + G +Y G R + R +R
Sbjct: 457 -TDNNSKLGGDLCKIMREANQTIPPELMRFDRRSFGSHIRYGRGGGRGGFRGGRGRGGFR 515
Query: 367 GGQARY 384
GG+ Y
Sbjct: 516 GGRGGY 521
>sp|O00571|DDX3X_HUMAN DEAD-box protein 3, X-chromosomal (Helicase-like protein 2) (HLP2)
(DEAD-box, X isoform)
Length = 662
Score = 81.3 bits (199), Expect = 2e-15
Identities = 42/119 (35%), Positives = 72/119 (60%)
Frame = +1
Query: 16 FKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTGRCGRMGRQGIAVTFIK 195
F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR GR GR+G G+A +F
Sbjct: 487 FRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFN 546
Query: 196 PSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSGTRYAKDRLESNYRGG 372
+ N +D++ +L + +EVP ++ +++ + YK S +K S + GG
Sbjct: 547 ERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAYEHHYKGSSRGRSK---SSRFSGG 596
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,849,142
Number of Sequences: 369166
Number of extensions: 1452927
Number of successful extensions: 4584
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4344
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4543
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5684835195
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)