Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_J13
(604 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q61496|DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh) 102 1e-21
sp|P09052|VASA_DROME Vasa protein (Antigen Mab46F11) 98 2e-20
sp|Q64060|DDX4_RAT DEAD-box protein 4 (VASA homolog) (rVLG) 97 3e-20
sp|P24784|DBP1_YEAST Probable ATP-dependent RNA helicase DB... 96 5e-20
sp|P16381|PL10_MOUSE Putative ATP-dependent RNA helicase PL10 96 9e-20
sp|P24346|AN3_XENLA Putative ATP-dependent RNA helicase An3 95 1e-19
sp|Q62095|DDX3Y_MOUSE DEAD-box protein 3, Y-chromosomal (DE... 95 1e-19
sp|Q62167|DDX3X_MOUSE DEAD-box protein 3, X-chromosomal (DE... 95 2e-19
sp|O00571|DDX3X_HUMAN DEAD-box protein 3, X-chromosomal (He... 94 2e-19
sp|Q9NQI0|DDX4_HUMAN DEAD-box protein 4 (VASA homolog) 94 3e-19
>sp|Q61496|DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh)
Length = 702
Score = 102 bits (253), Expect = 1e-21
Identities = 53/137 (38%), Positives = 81/137 (59%), Gaps = 3/137 (2%)
Frame = +3
Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
+HGD QR+R+ G F+ C VLVATSVAARG+D+ +++ V+N PS +EY+HR G
Sbjct: 543 IHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIG 602
Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSS- 362
R GR G G A++F +D +N + +VK+L+ ++VP ++ EI + + SS
Sbjct: 603 RTGRCGNTGRAISFF-DTDSDNHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPPSFSSST 661
Query: 363 --GTRYAKDRLESNYRG 407
G +A NY+G
Sbjct: 662 RGGAVFASVDTRKNYQG 678
>sp|P09052|VASA_DROME Vasa protein (Antigen Mab46F11)
Length = 661
Score = 97.8 bits (242), Expect = 2e-20
Identities = 47/103 (45%), Positives = 69/103 (66%)
Frame = +3
Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
+HGD Q QR+ FKN + KVL+ATSVA+RG+D+ +I+ V+N PS ++Y+HR G
Sbjct: 519 IHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIG 578
Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFM 314
R GR+G G A +F P + + A D+VK+L +G+ VPDF+
Sbjct: 579 RTGRVGNNGRATSFFDP-EKDRAIAADLVKILEGSGQTVPDFL 620
>sp|Q64060|DDX4_RAT DEAD-box protein 4 (VASA homolog) (rVLG)
Length = 713
Score = 97.1 bits (240), Expect = 3e-20
Identities = 50/136 (36%), Positives = 79/136 (58%), Gaps = 2/136 (1%)
Frame = +3
Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
+HGD QR+R+ G F+ C VLVATSVAARG+D+ +++ V+N PS +EY+HR G
Sbjct: 555 IHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFNLPSTIDEYVHRIG 614
Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEIC--KDVGIQFKYKS 359
R GR G G A++F ++ +N + +VK+L+ ++VP ++ EI F +
Sbjct: 615 RTGRCGNTGRAISFF-DTESDNHLAQPLVKVLSDAQQDVPAWLEEIAFSSYAPPSFSNST 673
Query: 360 SGTRYAKDRLESNYRG 407
G +A N++G
Sbjct: 674 RGAVFASFDTRKNFQG 689
>sp|P24784|DBP1_YEAST Probable ATP-dependent RNA helicase DBP1 (Helicase CA1)
Length = 617
Score = 96.3 bits (238), Expect = 5e-20
Identities = 50/146 (34%), Positives = 81/146 (55%)
Frame = +3
Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
+HGD +Q +R+ FK N +LVAT+VAARG+D+ ++ V+N PS+ ++Y+HR G
Sbjct: 441 IHGDRTQAERERALSAFKANVADILVATAVAARGLDIPNVTHVINYDLPSDIDDYVHRIG 500
Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
R GR G G+A +F + N V+ ++++LN+ +EVP F++++ +
Sbjct: 501 RTGRAGNTGVATSFFNSN--NQNIVKGLMEILNEANQEVPTFLSDLSR------------ 546
Query: 366 TRYAKDRLESNYRGGQARYGGDRFES 443
N RGG+ R GG F S
Sbjct: 547 ---------QNSRGGRTRGGGGFFNS 563
>sp|P16381|PL10_MOUSE Putative ATP-dependent RNA helicase PL10
Length = 660
Score = 95.5 bits (236), Expect = 9e-20
Identities = 54/176 (30%), Positives = 88/176 (50%)
Frame = +3
Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
+HGD SQR R+ F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR G
Sbjct: 470 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 529
Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
R GR+G G+A +F + N +D++ +L + +EVP ++ +++ + YK
Sbjct: 530 RTGRVGNLGLATSFFNERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAFEHHYKGGS 583
Query: 366 TRYAKDRLESNYRGGQARYGGDRFESNXXXXXXXXXXXXXFESNYRDGQARYGGDR 533
+K R + R S+ SN R G +G R
Sbjct: 584 RGRSKSRFSGGFGARDYRQSSGASSSSFSSGR---------ASNSRSGGGSHGSSR 630
>sp|P24346|AN3_XENLA Putative ATP-dependent RNA helicase An3
Length = 697
Score = 95.1 bits (235), Expect = 1e-19
Identities = 47/132 (35%), Positives = 80/132 (60%)
Frame = +3
Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
+HGD SQR R+ F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR G
Sbjct: 512 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 571
Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
R GR+G G+A +F + N +D++ +L + +EVP ++ +++ + +KSS
Sbjct: 572 RTGRVGNLGLATSFFNEKNIN--ITKDLLDLLVEAKQEVPSWL----ENMAYEQHHKSSS 625
Query: 366 TRYAKDRLESNY 401
+K R +
Sbjct: 626 RGRSKSRFSGGF 637
>sp|Q62095|DDX3Y_MOUSE DEAD-box protein 3, Y-chromosomal (DEAD-box RNA helicase DEAD2)
(mDEAD2) (D1Pas1-related sequence 1)
Length = 658
Score = 95.1 bits (235), Expect = 1e-19
Identities = 55/191 (28%), Positives = 89/191 (46%)
Frame = +3
Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
+HGD SQ+ R+ F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR G
Sbjct: 470 IHGDRSQKDREEALHQFRSGRKPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 529
Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
R GR+G G+A +F + N +D++ +L + +EVP ++ + + + YK S
Sbjct: 530 RTGRVGNLGLATSFFNERNLN--ITKDLLDLLVEAKQEVPSWL----ESMAYEHHYKGSS 583
Query: 366 TRYAKDRLESNYRGGQARYGGDRFESNXXXXXXXXXXXXXFESNYRDGQARYGGDRLESN 545
+K R + R + N G YGG
Sbjct: 584 RGRSKSRFSGGFGARDYRQSSGSANAGFNSNRANSSRSSGSSHNRGFGGGGYGGFYNNDG 643
Query: 546 LGSDQTKDAED 578
G + A D
Sbjct: 644 YGGNYNSQAVD 654
>sp|Q62167|DDX3X_MOUSE DEAD-box protein 3, X-chromosomal (DEAD-box RNA helicase DEAD3)
(mDEAD3) (Embryonic RNA helicase) (D1Pas1-related
sequence 2)
Length = 662
Score = 94.7 bits (234), Expect = 2e-19
Identities = 49/135 (36%), Positives = 81/135 (60%)
Frame = +3
Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
+HGD SQR R+ F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR G
Sbjct: 471 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 530
Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
R GR+G G+A +F + N +D++ +L + +EVP ++ +++ + YK S
Sbjct: 531 RTGRVGNLGLATSFFNERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAFEHHYKGSS 584
Query: 366 TRYAKDRLESNYRGG 410
+K S + GG
Sbjct: 585 RGRSK---SSRFSGG 596
>sp|O00571|DDX3X_HUMAN DEAD-box protein 3, X-chromosomal (Helicase-like protein 2) (HLP2)
(DEAD-box, X isoform)
Length = 662
Score = 94.4 bits (233), Expect = 2e-19
Identities = 49/135 (36%), Positives = 81/135 (60%)
Frame = +3
Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
+HGD SQR R+ F++ +LVAT+VAARG+D+S+++ V+N PS+ EEY+HR G
Sbjct: 471 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 530
Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
R GR+G G+A +F + N +D++ +L + +EVP ++ +++ + YK S
Sbjct: 531 RTGRVGNLGLATSFFNERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAYEHHYKGSS 584
Query: 366 TRYAKDRLESNYRGG 410
+K S + GG
Sbjct: 585 RGRSK---SSRFSGG 596
>sp|Q9NQI0|DDX4_HUMAN DEAD-box protein 4 (VASA homolog)
Length = 724
Score = 94.0 bits (232), Expect = 3e-19
Identities = 45/106 (42%), Positives = 68/106 (64%)
Frame = +3
Query: 6 LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
+HGD QR+R+ G F+ C VLVATSVAARG+D+ +++ V+N PS +EY+HR G
Sbjct: 570 IHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIG 629
Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEI 323
R GR G G A++F + +N + +VK+L ++VP ++ EI
Sbjct: 630 RTGRCGNTGRAISFF-DLESDNHLAQPLVKVLTDAQQDVPAWLEEI 674
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,387,876
Number of Sequences: 369166
Number of extensions: 1342035
Number of successful extensions: 4574
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4271
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4527
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4650991475
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)