Planarian EST Database


Dr_sW_024_J13

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_024_J13
         (604 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q61496|DDX4_MOUSE  DEAD-box protein 4 (VASA homolog) (Mvh)     102   1e-21
sp|P09052|VASA_DROME  Vasa protein (Antigen Mab46F11)              98   2e-20
sp|Q64060|DDX4_RAT  DEAD-box protein 4 (VASA homolog) (rVLG)       97   3e-20
sp|P24784|DBP1_YEAST  Probable ATP-dependent RNA helicase DB...    96   5e-20
sp|P16381|PL10_MOUSE  Putative ATP-dependent RNA helicase PL10     96   9e-20
sp|P24346|AN3_XENLA  Putative ATP-dependent RNA helicase An3       95   1e-19
sp|Q62095|DDX3Y_MOUSE  DEAD-box protein 3, Y-chromosomal (DE...    95   1e-19
sp|Q62167|DDX3X_MOUSE  DEAD-box protein 3, X-chromosomal (DE...    95   2e-19
sp|O00571|DDX3X_HUMAN  DEAD-box protein 3, X-chromosomal (He...    94   2e-19
sp|Q9NQI0|DDX4_HUMAN  DEAD-box protein 4 (VASA homolog)            94   3e-19
>sp|Q61496|DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh)
          Length = 702

 Score =  102 bits (253), Expect = 1e-21
 Identities = 53/137 (38%), Positives = 81/137 (59%), Gaps = 3/137 (2%)
 Frame = +3

Query: 6   LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
           +HGD  QR+R+   G F+   C VLVATSVAARG+D+ +++ V+N   PS  +EY+HR G
Sbjct: 543 IHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIG 602

Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSS- 362
           R GR G  G A++F   +D +N   + +VK+L+   ++VP ++ EI     +   + SS 
Sbjct: 603 RTGRCGNTGRAISFF-DTDSDNHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPPSFSSST 661

Query: 363 --GTRYAKDRLESNYRG 407
             G  +A      NY+G
Sbjct: 662 RGGAVFASVDTRKNYQG 678
>sp|P09052|VASA_DROME Vasa protein (Antigen Mab46F11)
          Length = 661

 Score = 97.8 bits (242), Expect = 2e-20
 Identities = 47/103 (45%), Positives = 69/103 (66%)
 Frame = +3

Query: 6   LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
           +HGD  Q QR+     FKN + KVL+ATSVA+RG+D+ +I+ V+N   PS  ++Y+HR G
Sbjct: 519 IHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIG 578

Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFM 314
           R GR+G  G A +F  P + + A   D+VK+L  +G+ VPDF+
Sbjct: 579 RTGRVGNNGRATSFFDP-EKDRAIAADLVKILEGSGQTVPDFL 620
>sp|Q64060|DDX4_RAT DEAD-box protein 4 (VASA homolog) (rVLG)
          Length = 713

 Score = 97.1 bits (240), Expect = 3e-20
 Identities = 50/136 (36%), Positives = 79/136 (58%), Gaps = 2/136 (1%)
 Frame = +3

Query: 6   LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
           +HGD  QR+R+   G F+   C VLVATSVAARG+D+ +++ V+N   PS  +EY+HR G
Sbjct: 555 IHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFNLPSTIDEYVHRIG 614

Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEIC--KDVGIQFKYKS 359
           R GR G  G A++F   ++ +N   + +VK+L+   ++VP ++ EI         F   +
Sbjct: 615 RTGRCGNTGRAISFF-DTESDNHLAQPLVKVLSDAQQDVPAWLEEIAFSSYAPPSFSNST 673

Query: 360 SGTRYAKDRLESNYRG 407
            G  +A      N++G
Sbjct: 674 RGAVFASFDTRKNFQG 689
>sp|P24784|DBP1_YEAST Probable ATP-dependent RNA helicase DBP1 (Helicase CA1)
          Length = 617

 Score = 96.3 bits (238), Expect = 5e-20
 Identities = 50/146 (34%), Positives = 81/146 (55%)
 Frame = +3

Query: 6   LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
           +HGD +Q +R+     FK N   +LVAT+VAARG+D+ ++  V+N   PS+ ++Y+HR G
Sbjct: 441 IHGDRTQAERERALSAFKANVADILVATAVAARGLDIPNVTHVINYDLPSDIDDYVHRIG 500

Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
           R GR G  G+A +F   +  N   V+ ++++LN+  +EVP F++++ +            
Sbjct: 501 RTGRAGNTGVATSFFNSN--NQNIVKGLMEILNEANQEVPTFLSDLSR------------ 546

Query: 366 TRYAKDRLESNYRGGQARYGGDRFES 443
                     N RGG+ R GG  F S
Sbjct: 547 ---------QNSRGGRTRGGGGFFNS 563
>sp|P16381|PL10_MOUSE Putative ATP-dependent RNA helicase PL10
          Length = 660

 Score = 95.5 bits (236), Expect = 9e-20
 Identities = 54/176 (30%), Positives = 88/176 (50%)
 Frame = +3

Query: 6   LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
           +HGD SQR R+     F++    +LVAT+VAARG+D+S+++ V+N   PS+ EEY+HR G
Sbjct: 470 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 529

Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
           R GR+G  G+A +F    + N    +D++ +L +  +EVP ++    +++  +  YK   
Sbjct: 530 RTGRVGNLGLATSFFNERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAFEHHYKGGS 583

Query: 366 TRYAKDRLESNYRGGQARYGGDRFESNXXXXXXXXXXXXXFESNYRDGQARYGGDR 533
              +K R    +     R       S+               SN R G   +G  R
Sbjct: 584 RGRSKSRFSGGFGARDYRQSSGASSSSFSSGR---------ASNSRSGGGSHGSSR 630
>sp|P24346|AN3_XENLA Putative ATP-dependent RNA helicase An3
          Length = 697

 Score = 95.1 bits (235), Expect = 1e-19
 Identities = 47/132 (35%), Positives = 80/132 (60%)
 Frame = +3

Query: 6   LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
           +HGD SQR R+     F++    +LVAT+VAARG+D+S+++ V+N   PS+ EEY+HR G
Sbjct: 512 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 571

Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
           R GR+G  G+A +F    + N    +D++ +L +  +EVP ++    +++  +  +KSS 
Sbjct: 572 RTGRVGNLGLATSFFNEKNIN--ITKDLLDLLVEAKQEVPSWL----ENMAYEQHHKSSS 625

Query: 366 TRYAKDRLESNY 401
              +K R    +
Sbjct: 626 RGRSKSRFSGGF 637
>sp|Q62095|DDX3Y_MOUSE DEAD-box protein 3, Y-chromosomal (DEAD-box RNA helicase DEAD2)
            (mDEAD2) (D1Pas1-related sequence 1)
          Length = 658

 Score = 95.1 bits (235), Expect = 1e-19
 Identities = 55/191 (28%), Positives = 89/191 (46%)
 Frame = +3

Query: 6    LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
            +HGD SQ+ R+     F++    +LVAT+VAARG+D+S+++ V+N   PS+ EEY+HR G
Sbjct: 470  IHGDRSQKDREEALHQFRSGRKPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 529

Query: 186  RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
            R GR+G  G+A +F    + N    +D++ +L +  +EVP ++    + +  +  YK S 
Sbjct: 530  RTGRVGNLGLATSFFNERNLN--ITKDLLDLLVEAKQEVPSWL----ESMAYEHHYKGSS 583

Query: 366  TRYAKDRLESNYRGGQARYGGDRFESNXXXXXXXXXXXXXFESNYRDGQARYGGDRLESN 545
               +K R    +     R       +                 N   G   YGG      
Sbjct: 584  RGRSKSRFSGGFGARDYRQSSGSANAGFNSNRANSSRSSGSSHNRGFGGGGYGGFYNNDG 643

Query: 546  LGSDQTKDAED 578
             G +    A D
Sbjct: 644  YGGNYNSQAVD 654
>sp|Q62167|DDX3X_MOUSE DEAD-box protein 3, X-chromosomal (DEAD-box RNA helicase DEAD3)
           (mDEAD3) (Embryonic RNA helicase) (D1Pas1-related
           sequence 2)
          Length = 662

 Score = 94.7 bits (234), Expect = 2e-19
 Identities = 49/135 (36%), Positives = 81/135 (60%)
 Frame = +3

Query: 6   LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
           +HGD SQR R+     F++    +LVAT+VAARG+D+S+++ V+N   PS+ EEY+HR G
Sbjct: 471 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 530

Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
           R GR+G  G+A +F    + N    +D++ +L +  +EVP ++    +++  +  YK S 
Sbjct: 531 RTGRVGNLGLATSFFNERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAFEHHYKGSS 584

Query: 366 TRYAKDRLESNYRGG 410
              +K    S + GG
Sbjct: 585 RGRSK---SSRFSGG 596
>sp|O00571|DDX3X_HUMAN DEAD-box protein 3, X-chromosomal (Helicase-like protein 2) (HLP2)
           (DEAD-box, X isoform)
          Length = 662

 Score = 94.4 bits (233), Expect = 2e-19
 Identities = 49/135 (36%), Positives = 81/135 (60%)
 Frame = +3

Query: 6   LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
           +HGD SQR R+     F++    +LVAT+VAARG+D+S+++ V+N   PS+ EEY+HR G
Sbjct: 471 IHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIG 530

Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEICKDVGIQFKYKSSG 365
           R GR+G  G+A +F    + N    +D++ +L +  +EVP ++    +++  +  YK S 
Sbjct: 531 RTGRVGNLGLATSFFNERNIN--ITKDLLDLLVEAKQEVPSWL----ENMAYEHHYKGSS 584

Query: 366 TRYAKDRLESNYRGG 410
              +K    S + GG
Sbjct: 585 RGRSK---SSRFSGG 596
>sp|Q9NQI0|DDX4_HUMAN DEAD-box protein 4 (VASA homolog)
          Length = 724

 Score = 94.0 bits (232), Expect = 3e-19
 Identities = 45/106 (42%), Positives = 68/106 (64%)
 Frame = +3

Query: 6   LHGDLSQRQRDVEFGLFKNNNCKVLVATSVAARGIDVSDIECVVNIGAPSNFEEYIHRTG 185
           +HGD  QR+R+   G F+   C VLVATSVAARG+D+ +++ V+N   PS  +EY+HR G
Sbjct: 570 IHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIG 629

Query: 186 RCGRMGRQGIAVTFIKPSDFNNACVRDIVKMLNKTGKEVPDFMNEI 323
           R GR G  G A++F    + +N   + +VK+L    ++VP ++ EI
Sbjct: 630 RTGRCGNTGRAISFF-DLESDNHLAQPLVKVLTDAQQDVPAWLEEI 674
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,387,876
Number of Sequences: 369166
Number of extensions: 1342035
Number of successful extensions: 4574
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4271
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4527
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4650991475
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)