Planaria EST Database

DrC_00963

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00963
         (762 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q99460|PSD1_HUMAN  26S proteasome non-ATPase regulatory s...   137   2e-32
sp|O88761|PSD1_RAT  26S proteasome non-ATPase regulatory sub...   137   2e-32
sp|Q9V3P6|PSD1_DROME  26S proteasome non-ATPase regulatory s...    89   2e-17
sp|O74762|RPN2_SCHPO  26S proteasome regulatory subunit rpn2       80   6e-15
sp|Q6FIP2|RPN2_CANGA  26S proteasome regulatory subunit RPN2       76   1e-13
sp|Q75CF3|RPN2_ASHGO  26S proteasome regulatory subunit RPN2       74   3e-13
sp|Q18115|PSD1_CAEEL  26S proteasome non-ATPase regulatory s...    74   6e-13
sp|P32565|RPN2_YEAST  26S proteasome regulatory subunit RPN2       69   1e-11
sp|P61328|FGF12_HUMAN  Fibroblast growth factor 12 (FGF-12) ...    30   7.1  
sp|P42527|KMHA_DICDI  Myosin heavy chain kinase A (MHCK A)         30   7.1  

>sp|Q99460|PSD1_HUMAN 26S proteasome non-ATPase regulatory subunit 1 (26S proteasome
            regulatory subunit RPN2) (26S proteasome regulatory
            subunit S1) (26S proteasome subunit p112)
          Length = 953

 Score =  137 bits (346), Expect = 2e-32
 Identities = 79/183 (43%), Positives = 107/183 (58%), Gaps = 3/183 (1%)
 Frame = +3

Query: 3    MTAAVGLLIFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDP 182
            M + VG+L+F  +WFW+PL++F+SLA+T T VI LNK+LKMPK++ KS  KPSTFAYP P
Sbjct: 757  MPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAP 816

Query: 183  IQIXXXXXXXXVETAVLSVXXXXXXXXXXXXXXXXXXEPMVVDTPTKLPE---KNDPELD 353
            +++        V TAVLS+                  E M VD   K  E   K +PE +
Sbjct: 817  LEVPKEKEKEKVSTAVLSI--TAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPN 874

Query: 354  FAILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIEHGGILVARDSQPNKPSDIVET 533
            F +L NPARVMP+QL+V+      T  E  RY P K +  GGI++ +D+      DI E 
Sbjct: 875  FQLLDNPARVMPAQLKVL------TMPETCRYQPFKPLSIGGIIILKDTS----EDIEEL 924

Query: 534  VKP 542
            V+P
Sbjct: 925  VEP 927

>sp|O88761|PSD1_RAT 26S proteasome non-ATPase regulatory subunit 1 (26S proteasome
            regulatory subunit RPN2) (26S proteasome regulatory
            subunit S1) (26S proteasome subunit p112)
          Length = 953

 Score =  137 bits (346), Expect = 2e-32
 Identities = 77/183 (42%), Positives = 108/183 (59%), Gaps = 3/183 (1%)
 Frame = +3

Query: 3    MTAAVGLLIFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDP 182
            M + VG+L+F  +WFW+PL++F+SLA+T T +I LNK+LKMPK++ KS  KPSTFAYP P
Sbjct: 757  MPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCIIGLNKDLKMPKVQYKSNCKPSTFAYPAP 816

Query: 183  IQIXXXXXXXXVETAVLSVXXXXXXXXXXXXXXXXXXEPMVVDTPTKLPE---KNDPELD 353
            +++        V TAVLS+                  E M VD   K  E   K +PE +
Sbjct: 817  LEVPKEKEKEKVSTAVLSI--TAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPN 874

Query: 354  FAILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIEHGGILVARDSQPNKPSDIVET 533
            F +L NPARVMP+QL+V+ ++      E  RY P K +  GGI++ +D+      DI E 
Sbjct: 875  FQLLDNPARVMPAQLKVLSMT------ETCRYQPFKPLSIGGIIILKDTS----EDIEEL 924

Query: 534  VKP 542
            V+P
Sbjct: 925  VEP 927

>sp|Q9V3P6|PSD1_DROME 26S proteasome non-ATPase regulatory subunit 1 (26S proteasome
            regulatory subunit RPN2) (26S proteasome regulatory
            complex subunit p110)
          Length = 1020

 Score = 88.6 bits (218), Expect = 2e-17
 Identities = 42/79 (53%), Positives = 54/79 (68%)
 Frame = +3

Query: 3    MTAAVGLLIFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDP 182
            + A VG+L F  YW+W+PL + +SLAFT T VI LN +LKMPK+E KS AKPS +AYP P
Sbjct: 773  LQAVVGMLAFTQYWYWFPLAHTLSLAFTPTCVIGLNSDLKMPKMEYKSAAKPSLYAYPAP 832

Query: 183  IQIXXXXXXXXVETAVLSV 239
            ++         V TAVLS+
Sbjct: 833  LEEKKSEEREKVATAVLSI 851


 Score = 43.9 bits (102), Expect = 5e-04
 Identities = 28/69 (40%), Positives = 40/69 (57%)
 Frame = +3

Query: 330  EKNDPELDFAILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIEHGGILVARDSQPN 509
            EK +PE    IL NPARV+  QL+V  LS ID     + Y P+K +  GGI+V + +   
Sbjct: 934  EKKEPEPTSEILQNPARVLRQQLKV--LSVIDG----QSYEPLKDVTIGGIIVFQHTGKA 987

Query: 510  KPSDIVETV 536
            +  ++VE V
Sbjct: 988  EDQELVEPV 996

>sp|O74762|RPN2_SCHPO 26S proteasome regulatory subunit rpn2
          Length = 965

 Score = 80.1 bits (196), Expect = 6e-15
 Identities = 59/200 (29%), Positives = 91/200 (45%), Gaps = 24/200 (12%)
 Frame = +3

Query: 3    MTAAVGLLIFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDP 182
            ++A VGL +F  YW+W+PLT+F+SL+F+ T++I L+K L  PK    S  +P  FAYP  
Sbjct: 740  LSAIVGLTVFLQYWYWFPLTHFMSLSFSPTALIGLDKNLNAPKFSFISNVRPKLFAYPPK 799

Query: 183  IQIXXXXXXXXVETAVLS----VXXXXXXXXXXXXXXXXXXEPMVVD---------TPTK 323
                       VETAVLS                       + M +D         TP +
Sbjct: 800  SVQPTAKTVQKVETAVLSTTVKAQARAKRAEREKASKGSNDDEMKIDKKTTEEKEATPME 859

Query: 324  LPEKNDPELDF-----------AILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIE 470
            + E+   ++               L N  RV+P+QL  I     + +G   RY P++   
Sbjct: 860  MDEEKSQDISINGNSKKEEPKSETLENFTRVVPAQLPYI---SFNLNG---RYYPVRKFT 913

Query: 471  HGGILVARDSQPNKPSDIVE 530
             GG+L+  D + +K  D++E
Sbjct: 914  -GGVLMLIDRESDKAPDLIE 932

>sp|Q6FIP2|RPN2_CANGA 26S proteasome regulatory subunit RPN2
          Length = 941

 Score = 75.9 bits (185), Expect = 1e-13
 Identities = 51/200 (25%), Positives = 96/200 (48%), Gaps = 21/200 (10%)
 Frame = +3

Query: 9    AAVGLLIFQNYWFWYPLTNFISLAFTLTSVI-VLNKELKMPKLEIKSKAKPSTFAYPDPI 185
            + VGL +F  +W+W+P+ +F+SL+FT T+++ V   +L +PK ++   A+   F+YP   
Sbjct: 726  SVVGLAMFTQFWYWFPMAHFLSLSFTPTTIVGVRGSDLNIPKFDMNCYAREDVFSYPKMF 785

Query: 186  QIXXXXXXXXVETAVLSVXXXXXXXXXXXXXXXXXXE----PMVVDTPTKLPEKND---- 341
            +         V TA+LS                   E        D   +  +K+D    
Sbjct: 786  EESADKEVEKVATAILSTTARAKARAKKTKKEKDTNEDDKKKKEKDLKKEETKKDDAKKE 845

Query: 342  --PELDF---------AILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIE-HGGIL 485
               E DF           + N +RV+P QL+ ++  + +      R+ P++  +   G++
Sbjct: 846  SEAEEDFNKNRYSSKPYKIENMSRVLPQQLKYVQFIKEE------RFTPVRKFKGTNGVV 899

Query: 486  VARDSQPNKPSDIVETVKPS 545
            V +D++P++P+ I+ETV+ S
Sbjct: 900  VLKDNKPSEPASIIETVRQS 919

>sp|Q75CF3|RPN2_ASHGO 26S proteasome regulatory subunit RPN2
          Length = 930

 Score = 74.3 bits (181), Expect = 3e-13
 Identities = 52/195 (26%), Positives = 91/195 (46%), Gaps = 18/195 (9%)
 Frame = +3

Query: 9    AAVGLLIFQNYWFWYPLTNFISLAFTLTSVI-VLNKELKMPKLEIKSKAKPSTFAYPDPI 185
            + +GL +F  +W+W+PL +F+SL+FT T+ I V + +LK+PK       K   F YP   
Sbjct: 721  SVIGLAMFTQFWYWFPLAHFLSLSFTPTTTIGVRSHDLKIPKFSFHCHTKEGIFDYPPMF 780

Query: 186  QIXXXXXXXXVETAVLSV-----XXXXXXXXXXXXXXXXXXEPMVVDTPTKLPEKND--- 341
            +         V TAVLS                        E + V+   +  +++D   
Sbjct: 781  EEDIDKSIEKVATAVLSTTAKAKARAKKSKKDKAVEPDKSKEEIKVENEQRDKKEHDADV 840

Query: 342  PELDFAI--------LTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIE-HGGILVAR 494
            PE +F I        + N  RV+P QL+ I   + +      R+ P++  +   G++V  
Sbjct: 841  PEEEFKIKYTSTYYKVENMTRVVPQQLKYIAFPKDE------RFTPVRKFKGSNGVIVLS 894

Query: 495  DSQPNKPSDIVETVK 539
            D  P++P ++++TV+
Sbjct: 895  DKTPDEPVEVIKTVR 909

>sp|Q18115|PSD1_CAEEL 26S proteasome non-ATPase regulatory subunit 1 (26S proteasome
            regulatory subunit rpn-2)
          Length = 965

 Score = 73.6 bits (179), Expect = 6e-13
 Identities = 56/208 (26%), Positives = 88/208 (42%), Gaps = 30/208 (14%)
 Frame = +3

Query: 3    MTAAVGLLIFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDP 182
            M + VG++ F + WFW+ +  FI+LA   + ++++N+ LK+P L+    A    FAYP  
Sbjct: 736  MGSMVGMMCFLHGWFWHSMHFFIALAAKPSCLVMMNENLKIPVLDYICHANSQKFAYPPR 795

Query: 183  IQIXXXXXXXXVETAVLSV---------------------------XXXXXXXXXXXXXX 281
             +         +ET VLS+                                         
Sbjct: 796  AESKKDKDVKKIETVVLSITGKKNASKKLIAEEKKRREAAASASSAAAAPSSSSTSGTAP 855

Query: 282  XXXXEPMVVDTPTKLPEKNDPELD---FAILTNPARVMPSQLRVIELSQIDTSGELRRYN 452
                E M VD P K  ++  PE D      L NPARV+P+Q ++I +S      + R Y+
Sbjct: 856  AAEDEKMEVDQPGKSKKEKAPEKDTKPLHRLQNPARVIPAQRQLISIS------DARAYS 909

Query: 453  PIKGIEHGGILVARDSQPNKPSDIVETV 536
            P+K +  GGI+VA      +   +V  V
Sbjct: 910  PMKPLYKGGIIVADRVDKEREEKLVSEV 937

>sp|P32565|RPN2_YEAST 26S proteasome regulatory subunit RPN2
          Length = 945

 Score = 69.3 bits (168), Expect = 1e-11
 Identities = 53/198 (26%), Positives = 87/198 (43%), Gaps = 21/198 (10%)
 Frame = +3

Query: 9    AAVGLLIFQNYWFWYPLTNFISLAFTLTSVI-VLNKELKMPKLEIKSKAKPSTFAYPDPI 185
            + VGL++F  +W+W+PL +F+SL+FT T+VI +   +  +PK ++   AK   F+YP   
Sbjct: 729  SVVGLVMFSQFWYWFPLAHFLSLSFTPTTVIGIRGSDQAIPKFQMNCYAKEDAFSYPRMY 788

Query: 186  QIXXXXXXXXVETAVLSVXXXXXXXXXXXXXXXXXXEPM------------------VVD 311
            +         V TAVLS                   E                    + +
Sbjct: 789  EEASGKEVEKVATAVLSTTARAKARAKKTKKEKGPNEEEKKKEHEEKEKERETNKKGIKE 848

Query: 312  TPTKLPE--KNDPELDFAILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIEHGGIL 485
            T     E  KN        + N  R++P Q R I   + D    +R++   KG  + G++
Sbjct: 849  TKENDEEFYKNKYSSKPYKVDNMTRILPQQSRYISFIKDDRFVPVRKF---KG--NNGVV 903

Query: 486  VARDSQPNKPSDIVETVK 539
            V RD +P +P  ++ETV+
Sbjct: 904  VLRDREPKEPVALIETVR 921

>sp|P61328|FGF12_HUMAN Fibroblast growth factor 12 (FGF-12) (Fibroblast growth factor
           homologous factor 1) (FHF-1) (Myocyte-activating factor)
 sp|P61150|FGF12_RAT Fibroblast growth factor 12 (FGF-12) (Fibroblast growth factor
           homologous factor 1) (FHF-1)
 sp|P61329|FGF12_MOUSE Fibroblast growth factor 12 (FGF-12) (Fibroblast growth factor
           homologous factor 1) (FHF-1) (Myocyte-activating factor)
          Length = 243

 Score = 30.0 bits (66), Expect = 7.1
 Identities = 17/55 (30%), Positives = 27/55 (49%)
 Frame = +3

Query: 27  IFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDPIQI 191
           +F+NY+  Y  T +          + LNKE ++ K     K KPS+   P PI++
Sbjct: 151 VFENYYVIYSSTLYRQQESGRAWFLGLNKEGQIMKGNRVKKTKPSSHFVPKPIEV 205

>sp|P42527|KMHA_DICDI Myosin heavy chain kinase A (MHCK A)
          Length = 1146

 Score = 30.0 bits (66), Expect = 7.1
 Identities = 14/63 (22%), Positives = 31/63 (49%)
 Frame = +3

Query: 330 EKNDPELDFAILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIEHGGILVARDSQPN 509
           EK   E +  +  +    +  ++++IE  Q+DTS E+R+   ++    G ++   +    
Sbjct: 284 EKKKSEQNQLLFDSKIESLKDKIKIIETQQLDTSSEVRKLK-LESTSSGNLMAGLNGTSG 342

Query: 510 KPS 518
           +PS
Sbjct: 343 RPS 345

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,271,205
Number of Sequences: 369166
Number of extensions: 1158740
Number of successful extensions: 3125
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3032
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3114
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7018522000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00963

  1. Dr_sW_024_E20
  2. Dr_sW_015_P20