Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_E20
(578 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99460|PSD1_HUMAN 26S proteasome non-ATPase regulatory s... 138 1e-32
sp|O88761|PSD1_RAT 26S proteasome non-ATPase regulatory sub... 138 1e-32
sp|Q9V3P6|PSD1_DROME 26S proteasome non-ATPase regulatory s... 89 1e-17
sp|O74762|RPN2_SCHPO 26S proteasome regulatory subunit rpn2 81 2e-15
sp|Q6FIP2|RPN2_CANGA 26S proteasome regulatory subunit RPN2 76 7e-14
sp|Q75CF3|RPN2_ASHGO 26S proteasome regulatory subunit RPN2 75 9e-14
sp|Q18115|PSD1_CAEEL 26S proteasome non-ATPase regulatory s... 74 3e-13
sp|P32565|RPN2_YEAST 26S proteasome regulatory subunit RPN2 69 6e-12
sp|P34940|CH60_PLAFG Chaperonin CPN60, mitochondrial precursor 30 4.3
sp|P61328|FGF12_HUMAN Fibroblast growth factor 12 (FGF-12) ... 30 4.3
>sp|Q99460|PSD1_HUMAN 26S proteasome non-ATPase regulatory subunit 1 (26S proteasome
regulatory subunit RPN2) (26S proteasome regulatory
subunit S1) (26S proteasome subunit p112)
Length = 953
Score = 138 bits (347), Expect = 1e-32
Identities = 79/195 (40%), Positives = 112/195 (57%), Gaps = 3/195 (1%)
Frame = +3
Query: 3 MTAAVGLLIFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDP 182
M + VG+L+F +WFW+PL++F+SLA+T T VI LNK+LKMPK++ KS KPSTFAYP P
Sbjct: 757 MPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAP 816
Query: 183 IQIXXXXXXXXVETAVLSVXXXXXXXXXXXXXXXXXXEPMVVDTPTKLPE---KNDPELD 353
+++ V TAVLS+ E M VD K E K +PE +
Sbjct: 817 LEVPKEKEKEKVSTAVLSI--TAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPN 874
Query: 354 FAILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIEHGGILVARDSQPNKPSDIVET 533
F +L NPARVMP+QL+V+ T E RY P K + GGI++ +D+ DI E
Sbjct: 875 FQLLDNPARVMPAQLKVL------TMPETCRYQPFKPLSIGGIIILKDTS----EDIEEL 924
Query: 534 VKPSLHRDDETDDDD 578
V+P + ++++
Sbjct: 925 VEPVAAHGPKIEEEE 939
>sp|O88761|PSD1_RAT 26S proteasome non-ATPase regulatory subunit 1 (26S proteasome
regulatory subunit RPN2) (26S proteasome regulatory
subunit S1) (26S proteasome subunit p112)
Length = 953
Score = 138 bits (347), Expect = 1e-32
Identities = 77/195 (39%), Positives = 113/195 (57%), Gaps = 3/195 (1%)
Frame = +3
Query: 3 MTAAVGLLIFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDP 182
M + VG+L+F +WFW+PL++F+SLA+T T +I LNK+LKMPK++ KS KPSTFAYP P
Sbjct: 757 MPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCIIGLNKDLKMPKVQYKSNCKPSTFAYPAP 816
Query: 183 IQIXXXXXXXXVETAVLSVXXXXXXXXXXXXXXXXXXEPMVVDTPTKLPE---KNDPELD 353
+++ V TAVLS+ E M VD K E K +PE +
Sbjct: 817 LEVPKEKEKEKVSTAVLSI--TAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPN 874
Query: 354 FAILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIEHGGILVARDSQPNKPSDIVET 533
F +L NPARVMP+QL+V+ ++ E RY P K + GGI++ +D+ DI E
Sbjct: 875 FQLLDNPARVMPAQLKVLSMT------ETCRYQPFKPLSIGGIIILKDTS----EDIEEL 924
Query: 534 VKPSLHRDDETDDDD 578
V+P + ++++
Sbjct: 925 VEPVAAHGPKIEEEE 939
>sp|Q9V3P6|PSD1_DROME 26S proteasome non-ATPase regulatory subunit 1 (26S proteasome
regulatory subunit RPN2) (26S proteasome regulatory
complex subunit p110)
Length = 1020
Score = 88.6 bits (218), Expect = 1e-17
Identities = 42/79 (53%), Positives = 54/79 (68%)
Frame = +3
Query: 3 MTAAVGLLIFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDP 182
+ A VG+L F YW+W+PL + +SLAFT T VI LN +LKMPK+E KS AKPS +AYP P
Sbjct: 773 LQAVVGMLAFTQYWYWFPLAHTLSLAFTPTCVIGLNSDLKMPKMEYKSAAKPSLYAYPAP 832
Query: 183 IQIXXXXXXXXVETAVLSV 239
++ V TAVLS+
Sbjct: 833 LEEKKSEEREKVATAVLSI 851
Score = 44.7 bits (104), Expect = 2e-04
Identities = 30/83 (36%), Positives = 45/83 (54%)
Frame = +3
Query: 330 EKNDPELDFAILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIEHGGILVARDSQPN 509
EK +PE IL NPARV+ QL+V LS ID + Y P+K + GGI+V + +
Sbjct: 934 EKKEPEPTSEILQNPARVLRQQLKV--LSVIDG----QSYEPLKDVTIGGIIVFQHTGKA 987
Query: 510 KPSDIVETVKPSLHRDDETDDDD 578
+ ++VE V +DE + +
Sbjct: 988 EDQELVEPVAAFGPMNDEEKEPE 1010
>sp|O74762|RPN2_SCHPO 26S proteasome regulatory subunit rpn2
Length = 965
Score = 81.3 bits (199), Expect = 2e-15
Identities = 60/213 (28%), Positives = 97/213 (45%), Gaps = 24/213 (11%)
Frame = +3
Query: 3 MTAAVGLLIFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDP 182
++A VGL +F YW+W+PLT+F+SL+F+ T++I L+K L PK S +P FAYP
Sbjct: 740 LSAIVGLTVFLQYWYWFPLTHFMSLSFSPTALIGLDKNLNAPKFSFISNVRPKLFAYPPK 799
Query: 183 IQIXXXXXXXXVETAVLS----VXXXXXXXXXXXXXXXXXXEPMVVD---------TPTK 323
VETAVLS + M +D TP +
Sbjct: 800 SVQPTAKTVQKVETAVLSTTVKAQARAKRAEREKASKGSNDDEMKIDKKTTEEKEATPME 859
Query: 324 LPEKNDPELDF-----------AILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIE 470
+ E+ ++ L N RV+P+QL I + +G RY P++
Sbjct: 860 MDEEKSQDISINGNSKKEEPKSETLENFTRVVPAQLPYI---SFNLNG---RYYPVRKFT 913
Query: 471 HGGILVARDSQPNKPSDIVETVKPSLHRDDETD 569
GG+L+ D + +K D++E + ++ +T+
Sbjct: 914 -GGVLMLIDRESDKAPDLIELNRDAVPASADTE 945
>sp|Q6FIP2|RPN2_CANGA 26S proteasome regulatory subunit RPN2
Length = 941
Score = 75.9 bits (185), Expect = 7e-14
Identities = 51/200 (25%), Positives = 96/200 (48%), Gaps = 21/200 (10%)
Frame = +3
Query: 9 AAVGLLIFQNYWFWYPLTNFISLAFTLTSVI-VLNKELKMPKLEIKSKAKPSTFAYPDPI 185
+ VGL +F +W+W+P+ +F+SL+FT T+++ V +L +PK ++ A+ F+YP
Sbjct: 726 SVVGLAMFTQFWYWFPMAHFLSLSFTPTTIVGVRGSDLNIPKFDMNCYAREDVFSYPKMF 785
Query: 186 QIXXXXXXXXVETAVLSVXXXXXXXXXXXXXXXXXXE----PMVVDTPTKLPEKND---- 341
+ V TA+LS E D + +K+D
Sbjct: 786 EESADKEVEKVATAILSTTARAKARAKKTKKEKDTNEDDKKKKEKDLKKEETKKDDAKKE 845
Query: 342 --PELDF---------AILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIE-HGGIL 485
E DF + N +RV+P QL+ ++ + + R+ P++ + G++
Sbjct: 846 SEAEEDFNKNRYSSKPYKIENMSRVLPQQLKYVQFIKEE------RFTPVRKFKGTNGVV 899
Query: 486 VARDSQPNKPSDIVETVKPS 545
V +D++P++P+ I+ETV+ S
Sbjct: 900 VLKDNKPSEPASIIETVRQS 919
>sp|Q75CF3|RPN2_ASHGO 26S proteasome regulatory subunit RPN2
Length = 930
Score = 75.5 bits (184), Expect = 9e-14
Identities = 55/205 (26%), Positives = 96/205 (46%), Gaps = 18/205 (8%)
Frame = +3
Query: 9 AAVGLLIFQNYWFWYPLTNFISLAFTLTSVI-VLNKELKMPKLEIKSKAKPSTFAYPDPI 185
+ +GL +F +W+W+PL +F+SL+FT T+ I V + +LK+PK K F YP
Sbjct: 721 SVIGLAMFTQFWYWFPLAHFLSLSFTPTTTIGVRSHDLKIPKFSFHCHTKEGIFDYPPMF 780
Query: 186 QIXXXXXXXXVETAVLSV-----XXXXXXXXXXXXXXXXXXEPMVVDTPTKLPEKND--- 341
+ V TAVLS E + V+ + +++D
Sbjct: 781 EEDIDKSIEKVATAVLSTTAKAKARAKKSKKDKAVEPDKSKEEIKVENEQRDKKEHDADV 840
Query: 342 PELDFAI--------LTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIE-HGGILVAR 494
PE +F I + N RV+P QL+ I + + R+ P++ + G++V
Sbjct: 841 PEEEFKIKYTSTYYKVENMTRVVPQQLKYIAFPKDE------RFTPVRKFKGSNGVIVLS 894
Query: 495 DSQPNKPSDIVETVKPSLHRDDETD 569
D P++P ++++TV+ ++ ETD
Sbjct: 895 DKTPDEPVEVIKTVR----QEKETD 915
>sp|Q18115|PSD1_CAEEL 26S proteasome non-ATPase regulatory subunit 1 (26S proteasome
regulatory subunit rpn-2)
Length = 965
Score = 73.6 bits (179), Expect = 3e-13
Identities = 56/208 (26%), Positives = 88/208 (42%), Gaps = 30/208 (14%)
Frame = +3
Query: 3 MTAAVGLLIFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDP 182
M + VG++ F + WFW+ + FI+LA + ++++N+ LK+P L+ A FAYP
Sbjct: 736 MGSMVGMMCFLHGWFWHSMHFFIALAAKPSCLVMMNENLKIPVLDYICHANSQKFAYPPR 795
Query: 183 IQIXXXXXXXXVETAVLSV---------------------------XXXXXXXXXXXXXX 281
+ +ET VLS+
Sbjct: 796 AESKKDKDVKKIETVVLSITGKKNASKKLIAEEKKRREAAASASSAAAAPSSSSTSGTAP 855
Query: 282 XXXXEPMVVDTPTKLPEKNDPELD---FAILTNPARVMPSQLRVIELSQIDTSGELRRYN 452
E M VD P K ++ PE D L NPARV+P+Q ++I +S + R Y+
Sbjct: 856 AAEDEKMEVDQPGKSKKEKAPEKDTKPLHRLQNPARVIPAQRQLISIS------DARAYS 909
Query: 453 PIKGIEHGGILVARDSQPNKPSDIVETV 536
P+K + GGI+VA + +V V
Sbjct: 910 PMKPLYKGGIIVADRVDKEREEKLVSEV 937
>sp|P32565|RPN2_YEAST 26S proteasome regulatory subunit RPN2
Length = 945
Score = 69.3 bits (168), Expect = 6e-12
Identities = 53/198 (26%), Positives = 87/198 (43%), Gaps = 21/198 (10%)
Frame = +3
Query: 9 AAVGLLIFQNYWFWYPLTNFISLAFTLTSVI-VLNKELKMPKLEIKSKAKPSTFAYPDPI 185
+ VGL++F +W+W+PL +F+SL+FT T+VI + + +PK ++ AK F+YP
Sbjct: 729 SVVGLVMFSQFWYWFPLAHFLSLSFTPTTVIGIRGSDQAIPKFQMNCYAKEDAFSYPRMY 788
Query: 186 QIXXXXXXXXVETAVLSVXXXXXXXXXXXXXXXXXXEPM------------------VVD 311
+ V TAVLS E + +
Sbjct: 789 EEASGKEVEKVATAVLSTTARAKARAKKTKKEKGPNEEEKKKEHEEKEKERETNKKGIKE 848
Query: 312 TPTKLPE--KNDPELDFAILTNPARVMPSQLRVIELSQIDTSGELRRYNPIKGIEHGGIL 485
T E KN + N R++P Q R I + D +R++ KG + G++
Sbjct: 849 TKENDEEFYKNKYSSKPYKVDNMTRILPQQSRYISFIKDDRFVPVRKF---KG--NNGVV 903
Query: 486 VARDSQPNKPSDIVETVK 539
V RD +P +P ++ETV+
Sbjct: 904 VLRDREPKEPVALIETVR 921
>sp|P34940|CH60_PLAFG Chaperonin CPN60, mitochondrial precursor
Length = 700
Score = 30.0 bits (66), Expect = 4.3
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = +3
Query: 477 GILVARDSQPNKPSDIVETVKPSLHRDDETDDDD 578
GIL + + PN S T K LH D++TD+DD
Sbjct: 618 GILDSSINSPNYLSKHRRTYKHKLHDDEDTDEDD 651
>sp|P61328|FGF12_HUMAN Fibroblast growth factor 12 (FGF-12) (Fibroblast growth factor
homologous factor 1) (FHF-1) (Myocyte-activating factor)
sp|P61150|FGF12_RAT Fibroblast growth factor 12 (FGF-12) (Fibroblast growth factor
homologous factor 1) (FHF-1)
sp|P61329|FGF12_MOUSE Fibroblast growth factor 12 (FGF-12) (Fibroblast growth factor
homologous factor 1) (FHF-1) (Myocyte-activating factor)
Length = 243
Score = 30.0 bits (66), Expect = 4.3
Identities = 17/55 (30%), Positives = 27/55 (49%)
Frame = +3
Query: 27 IFQNYWFWYPLTNFISLAFTLTSVIVLNKELKMPKLEIKSKAKPSTFAYPDPIQI 191
+F+NY+ Y T + + LNKE ++ K K KPS+ P PI++
Sbjct: 151 VFENYYVIYSSTLYRQQESGRAWFLGLNKEGQIMKGNRVKKTKPSSHFVPKPIEV 205
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,557,242
Number of Sequences: 369166
Number of extensions: 884953
Number of successful extensions: 2512
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2443
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2498
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4259329035
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)