Planaria EST Database

DrC_00944

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00944
         (815 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q6P7W5|SEN2_MOUSE  tRNA-splicing endonuclease subunit Sen...    63   1e-09
sp|Q8NCE0|SEN2_HUMAN  tRNA-splicing endonuclease subunit Sen...    60   7e-09
sp|Q6LY59|ENDA_METMP  tRNA-splicing endonuclease (tRNA-intro...    57   8e-08
sp|Q74ZY5|SEN2_ASHGO  tRNA-splicing endonuclease subunit SEN...    53   1e-06
sp|P16658|SEN2_YEAST  tRNA-splicing endonuclease subunit SEN...    52   3e-06
sp|Q58819|ENDA_METJA  tRNA-splicing endonuclease (tRNA-intro...    49   2e-05
sp|Q97ZY3|ENDA_SULSO  tRNA-splicing endonuclease (tRNA-intro...    47   6e-05
sp|Q4JAF4|ENDA_SULAC  tRNA-splicing endonuclease (tRNA-intro...    47   6e-05
sp|Q8TFH7|SEN2_SCHPO  Probable tRNA-splicing endonuclease su...    45   3e-04
sp|Q975R3|ENDA_SULTO  tRNA-splicing endonuclease (tRNA-intro...    44   4e-04

>sp|Q6P7W5|SEN2_MOUSE tRNA-splicing endonuclease subunit Sen2 (tRNA-intron endonuclease
           Sen2)
          Length = 460

 Score = 62.8 bits (151), Expect = 1e-09
 Identities = 48/165 (29%), Positives = 83/165 (50%), Gaps = 6/165 (3%)
 Frame = +3

Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFG 446
           L LS  E  FL Y    L I  +  K+ +++ ++W          F + ++AYHY+RS G
Sbjct: 292 LQLSLEEAFFLAYALGCLSI--YYEKEPLTIVKLWQAFTAVQPT-FRTTYMAYHYFRSKG 348

Query: 447 YAVRPGLRFGSDYLLYIASADHSHASYSVSVKCV---LNGELIDSFTQEQLLDQVRVSHA 617
           +  + GL++G+D LLY       HASYSV ++ +     G L   F+ + L    RVS  
Sbjct: 349 WVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELLDDNYEGSLRRPFSWKSLAALSRVSGN 408

Query: 618 VLKKMVYCKVVINSSNPLTNL---TIINDCEVEEIHTSRFDPNSE 743
           V K+++ C ++  S+    ++     +   +V+E+  SR+  + E
Sbjct: 409 VSKELMLCYLIKPSTMTAEDMETPECMKRIQVQEVILSRWVSSRE 453

>sp|Q8NCE0|SEN2_HUMAN tRNA-splicing endonuclease subunit Sen2 (tRNA-intron endonuclease
           Sen2) (HsSen2 protein)
          Length = 465

 Score = 60.1 bits (144), Expect = 7e-09
 Identities = 48/165 (29%), Positives = 82/165 (49%), Gaps = 6/165 (3%)
 Frame = +3

Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFG 446
           L LS  E  FL Y    L I  +  K+ +++ ++W          F + ++AYHY+RS G
Sbjct: 297 LQLSLEEAFFLVYALGCLSI--YYEKEPLTIVKLWKAFTVVQPT-FRTTYMAYHYFRSKG 353

Query: 447 YAVRPGLRFGSDYLLYIASADHSHASYSVSVKCV---LNGELIDSFTQEQLLDQVRVSHA 617
           +  + GL++G+D LLY       HASYSV ++ V     G L    + + L    RVS  
Sbjct: 354 WVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVN 413

Query: 618 VLKKMVYCKVVINSS---NPLTNLTIINDCEVEEIHTSRFDPNSE 743
           V K+++ C ++  S+     + +   +   +V+E+  SR+  + E
Sbjct: 414 VSKELMLCYLIKPSTMTDKEMESPECMKRIKVQEVILSRWVSSRE 458

>sp|Q6LY59|ENDA_METMP tRNA-splicing endonuclease (tRNA-intron endonuclease)
          Length = 177

 Score = 56.6 bits (135), Expect = 8e-08
 Identities = 48/146 (32%), Positives = 79/146 (54%), Gaps = 8/146 (5%)
 Frame = +3

Query: 249 DTRDYLLILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISL-FVAY 425
           +  +  L LS VE ++L  V  N I +  +NKK++S EE++  +V +N  R + + ++AY
Sbjct: 36  ELHENFLSLSFVEGLYL--VSKNWISLRDKNKKLLSFEELF--DVAQNIDRKLCIRYLAY 91

Query: 426 HYYRSFGYAVRPGLRFGSDYLLYIAS-ADHSHASYSVSVKCVLNGELIDSFTQE------ 584
              R+ GY VR GL++GSD+ LY  S  D  H+ Y V V           F++E      
Sbjct: 92  KDLRNRGYTVRTGLKYGSDFRLYERSNIDEIHSRYLVKV-----------FSEEIPCEIS 140

Query: 585 QLLDQVRVSHAVLKKMVYCKVVINSS 662
           ++   VRV+H+V K+++   V  + S
Sbjct: 141 EITGFVRVAHSVRKELIIAIVDADGS 166

>sp|Q74ZY5|SEN2_ASHGO tRNA-splicing endonuclease subunit SEN2 (tRNA-intron endonuclease
           SEN2)
          Length = 356

 Score = 52.8 bits (125), Expect = 1e-06
 Identities = 23/47 (48%), Positives = 30/47 (63%)
 Frame = +3

Query: 399 RFISLFVAYHYYRSFGYAVRPGLRFGSDYLLYIASADHSHASYSVSV 539
           R   L+ AYH+YRS G+ VR G++FG D+LLY       HA +SV V
Sbjct: 237 RLCRLYAAYHHYRSHGWCVRSGIKFGCDFLLYRRGPPFHHAEFSVMV 283

>sp|P16658|SEN2_YEAST tRNA-splicing endonuclease subunit SEN2 (tRNA-intron endonuclease
           SEN2) (Splicing endonuclease protein 2)
          Length = 377

 Score = 51.6 bits (122), Expect = 3e-06
 Identities = 33/91 (36%), Positives = 43/91 (47%), Gaps = 2/91 (2%)
 Frame = +3

Query: 267 LILSEVEVVFLQYVFNNLIIM--CFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRS 440
           L L  VE +FL +    L I   C   K          D   K+   F+  +V YH+YRS
Sbjct: 218 LELMPVEAMFLTFALPVLDISPACLAGKLF------QFDAKYKDIHSFVRSYVIYHHYRS 271

Query: 441 FGYAVRPGLRFGSDYLLYIASADHSHASYSV 533
            G+ VR G++FG DYLLY       HA + V
Sbjct: 272 HGWCVRSGIKFGCDYLLYKRGPPFQHAEFCV 302

>sp|Q58819|ENDA_METJA tRNA-splicing endonuclease (tRNA-intron endonuclease)
          Length = 179

 Score = 48.9 bits (115), Expect = 2e-05
 Identities = 41/143 (28%), Positives = 71/143 (49%), Gaps = 2/143 (1%)
 Frame = +3

Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFG 446
           L LS VE ++L  +    + + +++ K +S EE++ +       R    ++ Y   R+ G
Sbjct: 43  LSLSLVEALYL--INLGWLEVKYKDNKPLSFEELY-EYARNVEERLCLKYLVYKDLRTRG 99

Query: 447 YAVRPGLRFGSDYLLYI--ASADHSHASYSVSVKCVLNGELIDSFTQEQLLDQVRVSHAV 620
           Y V+ GL++G+D+ LY   A+ D  H+ Y V V          SF   +L   VRV+H+V
Sbjct: 100 YIVKTGLKYGADFRLYERGANIDKEHSVYLVKV-----FPEDSSFLLSELTGFVRVAHSV 154

Query: 621 LKKMVYCKVVINSSNPLTNLTII 689
            KK++   V  +      N+T +
Sbjct: 155 RKKLLIAIVDADGDIVYYNMTYV 177

>sp|Q97ZY3|ENDA_SULSO tRNA-splicing endonuclease (tRNA-intron endonuclease)
          Length = 182

 Score = 47.0 bits (110), Expect = 6e-05
 Identities = 35/127 (27%), Positives = 63/127 (49%)
 Frame = +3

Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFG 446
           L LS +E V+L      LI +  +N  ++  ++++  +  K   +F  L+  Y   R  G
Sbjct: 47  LELSLIESVYLTK--KGLINVVDKNGDLLEYKKLYEYSAMKIN-KFEILYKVYEDLREKG 103

Query: 447 YAVRPGLRFGSDYLLYIASADHSHASYSVSVKCVLNGELIDSFTQEQLLDQVRVSHAVLK 626
           + VR G+++G+D+ +Y       HA Y      V+  ++ +  T  +LL   RVSH+  K
Sbjct: 104 FIVRSGVKYGADFAVYTLGPGLEHAPY-----VVIAVDIDEEITPHELLSFGRVSHSTKK 158

Query: 627 KMVYCKV 647
           ++V   V
Sbjct: 159 RLVLALV 165

>sp|Q4JAF4|ENDA_SULAC tRNA-splicing endonuclease (tRNA-intron endonuclease)
          Length = 181

 Score = 47.0 bits (110), Expect = 6e-05
 Identities = 38/136 (27%), Positives = 65/136 (47%), Gaps = 3/136 (2%)
 Frame = +3

Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIIS---LEEIWMDNVNKNGARFISLFVAYHYYR 437
           LILS +E ++L       II    + K+I    L +I M+N+     RF  L+  Y   R
Sbjct: 47  LILSLIEALYLS---KKKIIEVIRDGKVIDDALLYKIGMENI----PRFELLYTVYEDLR 99

Query: 438 SFGYAVRPGLRFGSDYLLYIASADHSHASYSVSVKCVLNGELIDSFTQEQLLDQVRVSHA 617
              + VR G+++G+D+ +Y  +    HA Y V     ++ E     +  ++L   RVSH+
Sbjct: 100 EKKFVVRSGMKYGADFAIYTVAPGLEHAPYLV---IAIDEE--QEISPNEILGFGRVSHS 154

Query: 618 VLKKMVYCKVVINSSN 665
             K ++    ++N  N
Sbjct: 155 TRKNLILS--IVNQRN 168

>sp|Q8TFH7|SEN2_SCHPO Probable tRNA-splicing endonuclease subunit sen2 (tRNA-intron
           endonuclease sen2)
          Length = 380

 Score = 44.7 bits (104), Expect = 3e-04
 Identities = 32/123 (26%), Positives = 61/123 (49%), Gaps = 2/123 (1%)
 Frame = +3

Query: 285 EVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFGYAVRPG 464
           E++ +  +F N++          SL++   D + +   +F++   AY Y+R  G+ V+ G
Sbjct: 212 ELLPILKLFANIVANSVALTHDYSLQQSHEDPIIEPDNKFLTELAAYFYFRQQGWVVKNG 271

Query: 465 LRFGSDYLLYIASADHSHASYSV-SVKCVLNGELID-SFTQEQLLDQVRVSHAVLKKMVY 638
            +F  D+LLY      SHA +++  + CV N +  +  + +   L+  RV   V K ++ 
Sbjct: 272 TKFSVDFLLYKKGPVFSHAEFAILLIPCVGNKQKYNMQWHEVHCLN--RVIAQVKKSLIL 329

Query: 639 CKV 647
           C V
Sbjct: 330 CYV 332

>sp|Q975R3|ENDA_SULTO tRNA-splicing endonuclease (tRNA-intron endonuclease)
          Length = 180

 Score = 44.3 bits (103), Expect = 4e-04
 Identities = 35/123 (28%), Positives = 60/123 (48%)
 Frame = +3

Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFG 446
           LILS +E V+L       I+    N + +  E ++   V +   RF  L+  Y   R  G
Sbjct: 46  LILSLIEGVYLVKKGKLEIV---SNGERLDFERLYQIGVTQI-PRFRILYSVYEDLREKG 101

Query: 447 YAVRPGLRFGSDYLLYIASADHSHASYSVSVKCVLNGELIDSFTQEQLLDQVRVSHAVLK 626
           Y VR G+++G+D+ +Y       HA Y V +    N ++    +  ++L   RVSH+  K
Sbjct: 102 YVVRSGIKYGADFAVYTIGPGIEHAPYLV-IALDENSQI----SSNEILGFGRVSHSTRK 156

Query: 627 KMV 635
           +++
Sbjct: 157 ELI 159

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,357,733
Number of Sequences: 369166
Number of extensions: 1793942
Number of successful extensions: 4611
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4456
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4609
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7811456130
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00944

  1. Dr_sW_023_O04
  2. Dr_sW_020_L12