Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_L12
(471 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6P7W5|SEN2_MOUSE tRNA-splicing endonuclease subunit Sen... 58 9e-09
sp|Q8NCE0|SEN2_HUMAN tRNA-splicing endonuclease subunit Sen... 57 3e-08
sp|Q74ZY5|SEN2_ASHGO tRNA-splicing endonuclease subunit SEN... 53 4e-07
sp|P16658|SEN2_YEAST tRNA-splicing endonuclease subunit SEN... 51 2e-06
sp|Q6LY59|ENDA_METMP tRNA-splicing endonuclease (tRNA-intro... 49 6e-06
sp|Q58819|ENDA_METJA tRNA-splicing endonuclease (tRNA-intro... 46 5e-05
sp|Q97ZY3|ENDA_SULSO tRNA-splicing endonuclease (tRNA-intro... 45 1e-04
sp|Q9YBF1|ENDA_AERPE tRNA-splicing endonuclease (tRNA-intro... 44 1e-04
sp|Q8TFH7|SEN2_SCHPO Probable tRNA-splicing endonuclease su... 44 2e-04
sp|Q9P6Y2|SEN2_NEUCR Probable tRNA-splicing endonuclease su... 42 7e-04
>sp|Q6P7W5|SEN2_MOUSE tRNA-splicing endonuclease subunit Sen2 (tRNA-intron endonuclease
Sen2)
Length = 460
Score = 58.2 bits (139), Expect = 9e-09
Identities = 37/120 (30%), Positives = 65/120 (54%), Gaps = 6/120 (5%)
Frame = +1
Query: 61 FISLFVAYHYYRSFGYAVRPGLRFGSDYLLYIASADHSHASYSVSVKCV---LNGELIDS 231
F + ++AYHY+RS G+ + GL++G+D LLY HASYSV ++ + G L
Sbjct: 334 FRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELLDDNYEGSLRRP 393
Query: 232 FTQEQLLDQVRVSHAVLKKMVYCKVVINSSNPLTNL---TIINDCEVEEIHTSRFDPNSE 402
F+ + L RVS V K+++ C ++ S+ ++ + +V+E+ SR+ + E
Sbjct: 394 FSWKSLAALSRVSGNVSKELMLCYLIKPSTMTAEDMETPECMKRIQVQEVILSRWVSSRE 453
>sp|Q8NCE0|SEN2_HUMAN tRNA-splicing endonuclease subunit Sen2 (tRNA-intron endonuclease
Sen2) (HsSen2 protein)
Length = 465
Score = 56.6 bits (135), Expect = 3e-08
Identities = 37/120 (30%), Positives = 64/120 (53%), Gaps = 6/120 (5%)
Frame = +1
Query: 61 FISLFVAYHYYRSFGYAVRPGLRFGSDYLLYIASADHSHASYSVSVKCV---LNGELIDS 231
F + ++AYHY+RS G+ + GL++G+D LLY HASYSV ++ V G L
Sbjct: 339 FRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRP 398
Query: 232 FTQEQLLDQVRVSHAVLKKMVYCKVVINSS---NPLTNLTIINDCEVEEIHTSRFDPNSE 402
+ + L RVS V K+++ C ++ S+ + + + +V+E+ SR+ + E
Sbjct: 399 LSWKSLAALSRVSVNVSKELMLCYLIKPSTMTDKEMESPECMKRIKVQEVILSRWVSSRE 458
>sp|Q74ZY5|SEN2_ASHGO tRNA-splicing endonuclease subunit SEN2 (tRNA-intron endonuclease
SEN2)
Length = 356
Score = 52.8 bits (125), Expect = 4e-07
Identities = 23/47 (48%), Positives = 30/47 (63%)
Frame = +1
Query: 58 RFISLFVAYHYYRSFGYAVRPGLRFGSDYLLYIASADHSHASYSVSV 198
R L+ AYH+YRS G+ VR G++FG D+LLY HA +SV V
Sbjct: 237 RLCRLYAAYHHYRSHGWCVRSGIKFGCDFLLYRRGPPFHHAEFSVMV 283
>sp|P16658|SEN2_YEAST tRNA-splicing endonuclease subunit SEN2 (tRNA-intron endonuclease
SEN2) (Splicing endonuclease protein 2)
Length = 377
Score = 50.8 bits (120), Expect = 2e-06
Identities = 21/44 (47%), Positives = 28/44 (63%)
Frame = +1
Query: 61 FISLFVAYHYYRSFGYAVRPGLRFGSDYLLYIASADHSHASYSV 192
F+ +V YH+YRS G+ VR G++FG DYLLY HA + V
Sbjct: 259 FVRSYVIYHHYRSHGWCVRSGIKFGCDYLLYKRGPPFQHAEFCV 302
>sp|Q6LY59|ENDA_METMP tRNA-splicing endonuclease (tRNA-intron endonuclease)
Length = 177
Score = 48.9 bits (115), Expect = 6e-06
Identities = 38/115 (33%), Positives = 63/115 (54%), Gaps = 8/115 (6%)
Frame = +1
Query: 1 KKIISLEEIWMDNVNKNGARFISL-FVAYHYYRSFGYAVRPGLRFGSDYLLYIAS-ADHS 174
KK++S EE++ +V +N R + + ++AY R+ GY VR GL++GSD+ LY S D
Sbjct: 65 KKLLSFEELF--DVAQNIDRKLCIRYLAYKDLRNRGYTVRTGLKYGSDFRLYERSNIDEI 122
Query: 175 HASYSVSVKCVLNGELIDSFTQE------QLLDQVRVSHAVLKKMVYCKVVINSS 321
H+ Y V V F++E ++ VRV+H+V K+++ V + S
Sbjct: 123 HSRYLVKV-----------FSEEIPCEISEITGFVRVAHSVRKELIIAIVDADGS 166
>sp|Q58819|ENDA_METJA tRNA-splicing endonuclease (tRNA-intron endonuclease)
Length = 179
Score = 45.8 bits (107), Expect = 5e-05
Identities = 35/117 (29%), Positives = 57/117 (48%), Gaps = 2/117 (1%)
Frame = +1
Query: 4 KIISLEEIWMDNVNKNGARFISLFVAYHYYRSFGYAVRPGLRFGSDYLLYI--ASADHSH 177
K +S EE++ + R ++ Y R+ GY V+ GL++G+D+ LY A+ D H
Sbjct: 67 KPLSFEELY-EYARNVEERLCLKYLVYKDLRTRGYIVKTGLKYGADFRLYERGANIDKEH 125
Query: 178 ASYSVSVKCVLNGELIDSFTQEQLLDQVRVSHAVLKKMVYCKVVINSSNPLTNLTII 348
+ Y V V SF +L VRV+H+V KK++ V + N+T +
Sbjct: 126 SVYLVKV-----FPEDSSFLLSELTGFVRVAHSVRKKLLIAIVDADGDIVYYNMTYV 177
>sp|Q97ZY3|ENDA_SULSO tRNA-splicing endonuclease (tRNA-intron endonuclease)
Length = 182
Score = 44.7 bits (104), Expect = 1e-04
Identities = 25/83 (30%), Positives = 42/83 (50%)
Frame = +1
Query: 58 RFISLFVAYHYYRSFGYAVRPGLRFGSDYLLYIASADHSHASYSVSVKCVLNGELIDSFT 237
+F L+ Y R G+ VR G+++G+D+ +Y HA Y V+ ++ + T
Sbjct: 88 KFEILYKVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPY-----VVIAVDIDEEIT 142
Query: 238 QEQLLDQVRVSHAVLKKMVYCKV 306
+LL RVSH+ K++V V
Sbjct: 143 PHELLSFGRVSHSTKKRLVLALV 165
>sp|Q9YBF1|ENDA_AERPE tRNA-splicing endonuclease (tRNA-intron endonuclease)
Length = 187
Score = 44.3 bits (103), Expect = 1e-04
Identities = 27/90 (30%), Positives = 43/90 (47%), Gaps = 6/90 (6%)
Frame = +1
Query: 46 KNGARFISLFVAYHYYRSFGYAVRPGLRFGSDYLLYIASADHSHASYSVSVKCVLNGELI 225
+ RF L+ Y R G+ VR GL+FGSD+ +Y HA + ++
Sbjct: 91 RGNPRFSMLYNIYRDLRERGFVVRSGLKFGSDFAVYRLGPGIDHAPF-----------IV 139
Query: 226 DSFTQEQLLDQV------RVSHAVLKKMVY 297
+++ E +D V R+SH+V KK V+
Sbjct: 140 HAYSPEDNIDPVEIVRAGRLSHSVRKKFVF 169
>sp|Q8TFH7|SEN2_SCHPO Probable tRNA-splicing endonuclease subunit sen2 (tRNA-intron
endonuclease sen2)
Length = 380
Score = 43.9 bits (102), Expect = 2e-04
Identities = 29/100 (29%), Positives = 52/100 (52%), Gaps = 2/100 (2%)
Frame = +1
Query: 13 SLEEIWMDNVNKNGARFISLFVAYHYYRSFGYAVRPGLRFGSDYLLYIASADHSHASYSV 192
SL++ D + + +F++ AY Y+R G+ V+ G +F D+LLY SHA +++
Sbjct: 235 SLQQSHEDPIIEPDNKFLTELAAYFYFRQQGWVVKNGTKFSVDFLLYKKGPVFSHAEFAI 294
Query: 193 -SVKCVLNGELID-SFTQEQLLDQVRVSHAVLKKMVYCKV 306
+ CV N + + + + L+ RV V K ++ C V
Sbjct: 295 LLIPCVGNKQKYNMQWHEVHCLN--RVIAQVKKSLILCYV 332
>sp|Q9P6Y2|SEN2_NEUCR Probable tRNA-splicing endonuclease subunit sen-2 (tRNA-intron
endonuclease sen-2)
Length = 633
Score = 42.0 bits (97), Expect = 7e-04
Identities = 23/85 (27%), Positives = 40/85 (47%), Gaps = 7/85 (8%)
Frame = +1
Query: 61 FISLFVAYHYYRSFGYAVRPGLRFGSDYLLYIASADHSHASYSVSVKCVLNGELIDSFTQ 240
F+ + YH++RS G+ R G++FG D+++Y H+ + + V + F
Sbjct: 505 FLVQYAVYHHFRSLGWVPRHGIKFGVDWIIYQRGPVFDHSEFGIMVVPSFSDPRWSEFEH 564
Query: 241 EQ-------LLDQVRVSHAVLKKMV 294
E+ L+ RV VLK +V
Sbjct: 565 EESKKTWSWLMGVNRVLSHVLKSLV 589
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,509,936
Number of Sequences: 369166
Number of extensions: 1074108
Number of successful extensions: 2877
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2817
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2876
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2733320975
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)