Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_O04
(815 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6P7W5|SEN2_MOUSE tRNA-splicing endonuclease subunit Sen... 63 1e-09
sp|Q8NCE0|SEN2_HUMAN tRNA-splicing endonuclease subunit Sen... 60 7e-09
sp|Q6LY59|ENDA_METMP tRNA-splicing endonuclease (tRNA-intro... 57 8e-08
sp|Q74ZY5|SEN2_ASHGO tRNA-splicing endonuclease subunit SEN... 53 1e-06
sp|P16658|SEN2_YEAST tRNA-splicing endonuclease subunit SEN... 52 3e-06
sp|Q58819|ENDA_METJA tRNA-splicing endonuclease (tRNA-intro... 49 2e-05
sp|Q97ZY3|ENDA_SULSO tRNA-splicing endonuclease (tRNA-intro... 47 6e-05
sp|Q4JAF4|ENDA_SULAC tRNA-splicing endonuclease (tRNA-intro... 47 6e-05
sp|Q8TFH7|SEN2_SCHPO Probable tRNA-splicing endonuclease su... 45 3e-04
sp|Q975R3|ENDA_SULTO tRNA-splicing endonuclease (tRNA-intro... 44 4e-04
>sp|Q6P7W5|SEN2_MOUSE tRNA-splicing endonuclease subunit Sen2 (tRNA-intron endonuclease
Sen2)
Length = 460
Score = 62.8 bits (151), Expect = 1e-09
Identities = 48/165 (29%), Positives = 83/165 (50%), Gaps = 6/165 (3%)
Frame = +3
Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFG 446
L LS E FL Y L I + K+ +++ ++W F + ++AYHY+RS G
Sbjct: 292 LQLSLEEAFFLAYALGCLSI--YYEKEPLTIVKLWQAFTAVQPT-FRTTYMAYHYFRSKG 348
Query: 447 YAVRPGLRFGSDYLLYIASADHSHASYSVSVKCV---LNGELIDSFTQEQLLDQVRVSHA 617
+ + GL++G+D LLY HASYSV ++ + G L F+ + L RVS
Sbjct: 349 WVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELLDDNYEGSLRRPFSWKSLAALSRVSGN 408
Query: 618 VLKKMVYCKVVINSSNPLTNL---TIINDCEVEEIHTSRFDPNSE 743
V K+++ C ++ S+ ++ + +V+E+ SR+ + E
Sbjct: 409 VSKELMLCYLIKPSTMTAEDMETPECMKRIQVQEVILSRWVSSRE 453
>sp|Q8NCE0|SEN2_HUMAN tRNA-splicing endonuclease subunit Sen2 (tRNA-intron endonuclease
Sen2) (HsSen2 protein)
Length = 465
Score = 60.1 bits (144), Expect = 7e-09
Identities = 48/165 (29%), Positives = 82/165 (49%), Gaps = 6/165 (3%)
Frame = +3
Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFG 446
L LS E FL Y L I + K+ +++ ++W F + ++AYHY+RS G
Sbjct: 297 LQLSLEEAFFLVYALGCLSI--YYEKEPLTIVKLWKAFTVVQPT-FRTTYMAYHYFRSKG 353
Query: 447 YAVRPGLRFGSDYLLYIASADHSHASYSVSVKCV---LNGELIDSFTQEQLLDQVRVSHA 617
+ + GL++G+D LLY HASYSV ++ V G L + + L RVS
Sbjct: 354 WVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVN 413
Query: 618 VLKKMVYCKVVINSS---NPLTNLTIINDCEVEEIHTSRFDPNSE 743
V K+++ C ++ S+ + + + +V+E+ SR+ + E
Sbjct: 414 VSKELMLCYLIKPSTMTDKEMESPECMKRIKVQEVILSRWVSSRE 458
>sp|Q6LY59|ENDA_METMP tRNA-splicing endonuclease (tRNA-intron endonuclease)
Length = 177
Score = 56.6 bits (135), Expect = 8e-08
Identities = 48/146 (32%), Positives = 79/146 (54%), Gaps = 8/146 (5%)
Frame = +3
Query: 249 DTRDYLLILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISL-FVAY 425
+ + L LS VE ++L V N I + +NKK++S EE++ +V +N R + + ++AY
Sbjct: 36 ELHENFLSLSFVEGLYL--VSKNWISLRDKNKKLLSFEELF--DVAQNIDRKLCIRYLAY 91
Query: 426 HYYRSFGYAVRPGLRFGSDYLLYIAS-ADHSHASYSVSVKCVLNGELIDSFTQE------ 584
R+ GY VR GL++GSD+ LY S D H+ Y V V F++E
Sbjct: 92 KDLRNRGYTVRTGLKYGSDFRLYERSNIDEIHSRYLVKV-----------FSEEIPCEIS 140
Query: 585 QLLDQVRVSHAVLKKMVYCKVVINSS 662
++ VRV+H+V K+++ V + S
Sbjct: 141 EITGFVRVAHSVRKELIIAIVDADGS 166
>sp|Q74ZY5|SEN2_ASHGO tRNA-splicing endonuclease subunit SEN2 (tRNA-intron endonuclease
SEN2)
Length = 356
Score = 52.8 bits (125), Expect = 1e-06
Identities = 23/47 (48%), Positives = 30/47 (63%)
Frame = +3
Query: 399 RFISLFVAYHYYRSFGYAVRPGLRFGSDYLLYIASADHSHASYSVSV 539
R L+ AYH+YRS G+ VR G++FG D+LLY HA +SV V
Sbjct: 237 RLCRLYAAYHHYRSHGWCVRSGIKFGCDFLLYRRGPPFHHAEFSVMV 283
>sp|P16658|SEN2_YEAST tRNA-splicing endonuclease subunit SEN2 (tRNA-intron endonuclease
SEN2) (Splicing endonuclease protein 2)
Length = 377
Score = 51.6 bits (122), Expect = 3e-06
Identities = 33/91 (36%), Positives = 43/91 (47%), Gaps = 2/91 (2%)
Frame = +3
Query: 267 LILSEVEVVFLQYVFNNLIIM--CFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRS 440
L L VE +FL + L I C K D K+ F+ +V YH+YRS
Sbjct: 218 LELMPVEAMFLTFALPVLDISPACLAGKLF------QFDAKYKDIHSFVRSYVIYHHYRS 271
Query: 441 FGYAVRPGLRFGSDYLLYIASADHSHASYSV 533
G+ VR G++FG DYLLY HA + V
Sbjct: 272 HGWCVRSGIKFGCDYLLYKRGPPFQHAEFCV 302
>sp|Q58819|ENDA_METJA tRNA-splicing endonuclease (tRNA-intron endonuclease)
Length = 179
Score = 48.9 bits (115), Expect = 2e-05
Identities = 41/143 (28%), Positives = 71/143 (49%), Gaps = 2/143 (1%)
Frame = +3
Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFG 446
L LS VE ++L + + + +++ K +S EE++ + R ++ Y R+ G
Sbjct: 43 LSLSLVEALYL--INLGWLEVKYKDNKPLSFEELY-EYARNVEERLCLKYLVYKDLRTRG 99
Query: 447 YAVRPGLRFGSDYLLYI--ASADHSHASYSVSVKCVLNGELIDSFTQEQLLDQVRVSHAV 620
Y V+ GL++G+D+ LY A+ D H+ Y V V SF +L VRV+H+V
Sbjct: 100 YIVKTGLKYGADFRLYERGANIDKEHSVYLVKV-----FPEDSSFLLSELTGFVRVAHSV 154
Query: 621 LKKMVYCKVVINSSNPLTNLTII 689
KK++ V + N+T +
Sbjct: 155 RKKLLIAIVDADGDIVYYNMTYV 177
>sp|Q97ZY3|ENDA_SULSO tRNA-splicing endonuclease (tRNA-intron endonuclease)
Length = 182
Score = 47.0 bits (110), Expect = 6e-05
Identities = 35/127 (27%), Positives = 63/127 (49%)
Frame = +3
Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFG 446
L LS +E V+L LI + +N ++ ++++ + K +F L+ Y R G
Sbjct: 47 LELSLIESVYLTK--KGLINVVDKNGDLLEYKKLYEYSAMKIN-KFEILYKVYEDLREKG 103
Query: 447 YAVRPGLRFGSDYLLYIASADHSHASYSVSVKCVLNGELIDSFTQEQLLDQVRVSHAVLK 626
+ VR G+++G+D+ +Y HA Y V+ ++ + T +LL RVSH+ K
Sbjct: 104 FIVRSGVKYGADFAVYTLGPGLEHAPY-----VVIAVDIDEEITPHELLSFGRVSHSTKK 158
Query: 627 KMVYCKV 647
++V V
Sbjct: 159 RLVLALV 165
>sp|Q4JAF4|ENDA_SULAC tRNA-splicing endonuclease (tRNA-intron endonuclease)
Length = 181
Score = 47.0 bits (110), Expect = 6e-05
Identities = 38/136 (27%), Positives = 65/136 (47%), Gaps = 3/136 (2%)
Frame = +3
Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIIS---LEEIWMDNVNKNGARFISLFVAYHYYR 437
LILS +E ++L II + K+I L +I M+N+ RF L+ Y R
Sbjct: 47 LILSLIEALYLS---KKKIIEVIRDGKVIDDALLYKIGMENI----PRFELLYTVYEDLR 99
Query: 438 SFGYAVRPGLRFGSDYLLYIASADHSHASYSVSVKCVLNGELIDSFTQEQLLDQVRVSHA 617
+ VR G+++G+D+ +Y + HA Y V ++ E + ++L RVSH+
Sbjct: 100 EKKFVVRSGMKYGADFAIYTVAPGLEHAPYLV---IAIDEE--QEISPNEILGFGRVSHS 154
Query: 618 VLKKMVYCKVVINSSN 665
K ++ ++N N
Sbjct: 155 TRKNLILS--IVNQRN 168
>sp|Q8TFH7|SEN2_SCHPO Probable tRNA-splicing endonuclease subunit sen2 (tRNA-intron
endonuclease sen2)
Length = 380
Score = 44.7 bits (104), Expect = 3e-04
Identities = 32/123 (26%), Positives = 61/123 (49%), Gaps = 2/123 (1%)
Frame = +3
Query: 285 EVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFGYAVRPG 464
E++ + +F N++ SL++ D + + +F++ AY Y+R G+ V+ G
Sbjct: 212 ELLPILKLFANIVANSVALTHDYSLQQSHEDPIIEPDNKFLTELAAYFYFRQQGWVVKNG 271
Query: 465 LRFGSDYLLYIASADHSHASYSV-SVKCVLNGELID-SFTQEQLLDQVRVSHAVLKKMVY 638
+F D+LLY SHA +++ + CV N + + + + L+ RV V K ++
Sbjct: 272 TKFSVDFLLYKKGPVFSHAEFAILLIPCVGNKQKYNMQWHEVHCLN--RVIAQVKKSLIL 329
Query: 639 CKV 647
C V
Sbjct: 330 CYV 332
>sp|Q975R3|ENDA_SULTO tRNA-splicing endonuclease (tRNA-intron endonuclease)
Length = 180
Score = 44.3 bits (103), Expect = 4e-04
Identities = 35/123 (28%), Positives = 60/123 (48%)
Frame = +3
Query: 267 LILSEVEVVFLQYVFNNLIIMCFENKKIISLEEIWMDNVNKNGARFISLFVAYHYYRSFG 446
LILS +E V+L I+ N + + E ++ V + RF L+ Y R G
Sbjct: 46 LILSLIEGVYLVKKGKLEIV---SNGERLDFERLYQIGVTQI-PRFRILYSVYEDLREKG 101
Query: 447 YAVRPGLRFGSDYLLYIASADHSHASYSVSVKCVLNGELIDSFTQEQLLDQVRVSHAVLK 626
Y VR G+++G+D+ +Y HA Y V + N ++ + ++L RVSH+ K
Sbjct: 102 YVVRSGIKYGADFAVYTIGPGIEHAPYLV-IALDENSQI----SSNEILGFGRVSHSTRK 156
Query: 627 KMV 635
+++
Sbjct: 157 ELI 159
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,357,733
Number of Sequences: 369166
Number of extensions: 1793942
Number of successful extensions: 4611
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4456
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4609
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7811456130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)