Planaria EST Database

DrC_00895

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00895
         (413 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9Y6A9|SPCS1_HUMAN  Signal peptidase complex subunit 1 (M...    48   9e-06
sp|Q9D958|SPCS1_MOUSE  Signal peptidase complex subunit 1 (M...    45   6e-05
sp|Q9VAL0|SPCS1_DROME  Probable signal peptidase complex sub...    44   1e-04
sp|Q61CQ8|SPCS1_CAEBR  Probable signal peptidase complex sub...    41   8e-04
sp|O44953|SPCS1_CAEEL  Probable signal peptidase complex sub...    40   0.001
sp|P67048|TYSY_STAAW  Thymidylate synthase (TS) (TSase) >gi|...    31   1.1  
sp|Q5HFZ6|TYSY_STAAC  Thymidylate synthase (TS) (TSase) >gi|...    31   1.1  
sp|P0C0M5|TYSY_STAES  Thymidylate synthase (TS) (TSase) >gi|...    30   1.9  
sp|Q944J0|SPCS1_ARATH  Probable signal peptidase complex sub...    30   2.5  
sp|Q81R23|TYSY_BACAN  Thymidylate synthase (TS) (TSase) >gi|...    29   4.3  

>sp|Q9Y6A9|SPCS1_HUMAN Signal peptidase complex subunit 1 (Microsomal signal peptidase 12
           kDa subunit) (SPase 12 kDa subunit)
 sp|Q5RF96|SPCS1_PONPY Signal peptidase complex subunit 1 (Microsomal signal peptidase 12
           kDa subunit) (SPase 12 kDa subunit)
          Length = 102

 Score = 47.8 bits (112), Expect = 9e-06
 Identities = 23/81 (28%), Positives = 33/81 (40%)
 Frame = +3

Query: 27  DRIESIPHHLDFVGQKFAEKXXXXXXXXXXXXXXXWGYYCQQISQTXXXXXXXXXXXXXX 206
           + + S+P  +D+ GQK AE+               +GY  +Q   T              
Sbjct: 3   EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLL 62

Query: 207 XXIPWPFFRRYPLNWQKVSES 269
              PWP +RR+PL W  V ES
Sbjct: 63  TLPPWPIYRRHPLKWLPVQES 83

>sp|Q9D958|SPCS1_MOUSE Signal peptidase complex subunit 1 (Microsomal signal peptidase 12
           kDa subunit) (SPase 12 kDa subunit)
          Length = 102

 Score = 45.1 bits (105), Expect = 6e-05
 Identities = 21/80 (26%), Positives = 32/80 (40%)
 Frame = +3

Query: 27  DRIESIPHHLDFVGQKFAEKXXXXXXXXXXXXXXXWGYYCQQISQTXXXXXXXXXXXXXX 206
           + + S+P  +D+ GQK AE+               +GY  +Q   T              
Sbjct: 3   EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLL 62

Query: 207 XXIPWPFFRRYPLNWQKVSE 266
              PWP +RR+PL W  V +
Sbjct: 63  TLPPWPIYRRHPLKWLPVQD 82

>sp|Q9VAL0|SPCS1_DROME Probable signal peptidase complex subunit 1 (Microsomal signal
           peptidase 12 kDa subunit) (SPase 12 kDa subunit)
          Length = 98

 Score = 43.9 bits (102), Expect = 1e-04
 Identities = 26/87 (29%), Positives = 36/87 (41%), Gaps = 3/87 (3%)
 Frame = +3

Query: 42  IPHHLDFVGQKFAEKXXXXXXXXXXXXXXXWGYYCQQISQTXXXXXXXXXXXXXXXXIPW 221
           I  H+DF GQ  AE+               +G + QQ SQT                 PW
Sbjct: 4   IQTHMDFAGQGKAERWSRFIITFFGIVGLVYGAFVQQFSQTVYILGAGFVLSSLITIPPW 63

Query: 222 PFFRRYPLNWQKVSESEL---HAEAGD 293
           P +RR  L WQK  +++     +E+GD
Sbjct: 64  PLYRRNALKWQKPIDTDAKSSSSESGD 90

>sp|Q61CQ8|SPCS1_CAEBR Probable signal peptidase complex subunit 1 (Microsomal signal
           peptidase 12 kDa subunit) (SPase 12 kDa subunit)
          Length = 113

 Score = 41.2 bits (95), Expect = 8e-04
 Identities = 24/88 (27%), Positives = 35/88 (39%), Gaps = 1/88 (1%)
 Frame = +3

Query: 33  IESIPHHLDFVGQKFAEKXXXXXXXXXXXXXXXWGYYCQQISQTXXXXXXXXXXXXXXXX 212
           ++ +  H+DF GQK AE+                GY  QQ+S                  
Sbjct: 12  LQQLSSHIDFQGQKVAERTYQVILTLAGIIGFFVGYSTQQLSYAMYTVMGAAVFTALIIL 71

Query: 213 IPWPF-FRRYPLNWQKVSESELHAEAGD 293
            PWPF FR+ P+ WQ   E +  + + D
Sbjct: 72  PPWPFLFRKNPIVWQTPIEEQEASSSSD 99

>sp|O44953|SPCS1_CAEEL Probable signal peptidase complex subunit 1 (Microsomal signal
           peptidase 12 kDa subunit) (SPase 12 kDa subunit)
          Length = 105

 Score = 40.4 bits (93), Expect = 0.001
 Identities = 24/88 (27%), Positives = 37/88 (42%), Gaps = 1/88 (1%)
 Frame = +3

Query: 33  IESIPHHLDFVGQKFAEKXXXXXXXXXXXXXXXWGYYCQQISQTXXXXXXXXXXXXXXXX 212
           ++ +  H+DF GQK AE+                G++ QQ+S                  
Sbjct: 12  LQKLSSHIDFQGQKVAERTYQVILTIAGIIGFLVGFWTQQLSYAMFTVLGASAFTALIIL 71

Query: 213 IPWPF-FRRYPLNWQKVSESELHAEAGD 293
            PWPF FR+ P+ W   +E +   E+GD
Sbjct: 72  PPWPFLFRKNPIVWHTPAEPQ---ESGD 96

>sp|P67048|TYSY_STAAW Thymidylate synthase (TS) (TSase)
 sp|P67047|TYSY_STAAN Thymidylate synthase (TS) (TSase)
 sp|P67046|TYSY_STAAM Thymidylate synthase (TS) (TSase)
 sp|Q6G9D4|TYSY_STAAS Thymidylate synthase (TS) (TSase)
          Length = 318

 Score = 30.8 bits (68), Expect = 1.1
 Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
 Frame = +2

Query: 95  EYNSNLWNNWIYMGLLLST--NFPNFIGFGRRICS 193
           +YN+N+WN W +   + S   N P+   FG R  S
Sbjct: 79  KYNNNIWNEWAFENYIKSDEYNGPDMTDFGHRALS 113

>sp|Q5HFZ6|TYSY_STAAC Thymidylate synthase (TS) (TSase)
 sp|Q6GGY0|TYSY_STAAR Thymidylate synthase (TS) (TSase)
          Length = 318

 Score = 30.8 bits (68), Expect = 1.1
 Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
 Frame = +2

Query: 95  EYNSNLWNNWIYMGLLLSTNF--PNFIGFGRRICS 193
           +YN+N+WN W +   + S  +  P+   FG R  S
Sbjct: 79  KYNNNIWNEWAFENYIKSDEYKGPDMTDFGHRALS 113

>sp|P0C0M5|TYSY_STAES Thymidylate synthase (TS) (TSase)
 sp|P0A0M5|TYSY_STAAU Thymidylate synthase (TS) (TSase)
 sp|Q5HMP6|TYSY_STAEQ Thymidylate synthase (TS) (TSase)
          Length = 318

 Score = 30.0 bits (66), Expect = 1.9
 Identities = 11/32 (34%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
 Frame = +2

Query: 95  EYNSNLWNNWIYMGLLLSTNF--PNFIGFGRR 184
           +YN+N+WN W +   + S ++  P+   FG R
Sbjct: 79  KYNNNIWNEWAFENYVQSDDYHGPDMTDFGHR 110

>sp|Q944J0|SPCS1_ARATH Probable signal peptidase complex subunit 1 (Microsomal signal
           peptidase 12 kDa subunit) (SPase 12 kDa subunit)
          Length = 92

 Score = 29.6 bits (65), Expect = 2.5
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = +3

Query: 219 WPFFRRYPLNWQKVSESELH 278
           WPF+  +PL W   SE+E H
Sbjct: 56  WPFYNLHPLKWLDPSEAEKH 75

>sp|Q81R23|TYSY_BACAN Thymidylate synthase (TS) (TSase)
 sp|Q6HJC7|TYSY_BACHK Thymidylate synthase (TS) (TSase)
          Length = 318

 Score = 28.9 bits (63), Expect = 4.3
 Identities = 11/32 (34%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
 Frame = +2

Query: 95  EYNSNLWNNWIYMGLLLSTNF--PNFIGFGRR 184
           ++N+N+WN W +   + S  +  P+ I FG R
Sbjct: 78  QHNNNIWNEWAFKSWVESDEYTGPDMIDFGLR 109

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,444,161
Number of Sequences: 369166
Number of extensions: 627675
Number of successful extensions: 1310
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1293
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1307
length of database: 68,354,980
effective HSP length: 99
effective length of database: 50,066,215
effective search space used: 1902516170
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00895

  1. Dr_sW_022_J08
  2. Dr_sW_026_N01
  3. Dr_sW_006_I06
  4. Dr_sW_028_M04