Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_M04
(379 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9Y6A9|SPCS1_HUMAN Signal peptidase complex subunit 1 (M... 47 1e-05
sp|Q9D958|SPCS1_MOUSE Signal peptidase complex subunit 1 (M... 44 9e-05
sp|Q9VAL0|SPCS1_DROME Probable signal peptidase complex sub... 44 1e-04
sp|Q61CQ8|SPCS1_CAEBR Probable signal peptidase complex sub... 41 7e-04
sp|O44953|SPCS1_CAEEL Probable signal peptidase complex sub... 40 0.001
sp|P67048|TYSY_STAAW Thymidylate synthase (TS) (TSase) >gi|... 31 1.0
sp|Q5HFZ6|TYSY_STAAC Thymidylate synthase (TS) (TSase) >gi|... 31 1.0
sp|P0C0M5|TYSY_STAES Thymidylate synthase (TS) (TSase) >gi|... 30 1.7
sp|Q944J0|SPCS1_ARATH Probable signal peptidase complex sub... 30 2.2
sp|Q81R23|TYSY_BACAN Thymidylate synthase (TS) (TSase) >gi|... 29 3.8
>sp|Q9Y6A9|SPCS1_HUMAN Signal peptidase complex subunit 1 (Microsomal signal peptidase 12
kDa subunit) (SPase 12 kDa subunit)
sp|Q5RF96|SPCS1_PONPY Signal peptidase complex subunit 1 (Microsomal signal peptidase 12
kDa subunit) (SPase 12 kDa subunit)
Length = 102
Score = 47.0 bits (110), Expect = 1e-05
Identities = 23/79 (29%), Positives = 32/79 (40%)
Frame = +2
Query: 5 IESIPHHLDFVGQKFAEKXXXXXXXXXXXXXXXWGYYCQQISQTXXXXXXXXXXXXXXXX 184
+ S+P +D+ GQK AE+ +GY +Q T
Sbjct: 5 LSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTL 64
Query: 185 IPWPFFRRYPLNWQKVSES 241
PWP +RR+PL W V ES
Sbjct: 65 PPWPIYRRHPLKWLPVQES 83
>sp|Q9D958|SPCS1_MOUSE Signal peptidase complex subunit 1 (Microsomal signal peptidase 12
kDa subunit) (SPase 12 kDa subunit)
Length = 102
Score = 44.3 bits (103), Expect = 9e-05
Identities = 21/78 (26%), Positives = 31/78 (39%)
Frame = +2
Query: 5 IESIPHHLDFVGQKFAEKXXXXXXXXXXXXXXXWGYYCQQISQTXXXXXXXXXXXXXXXX 184
+ S+P +D+ GQK AE+ +GY +Q T
Sbjct: 5 LSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTL 64
Query: 185 IPWPFFRRYPLNWQKVSE 238
PWP +RR+PL W V +
Sbjct: 65 PPWPIYRRHPLKWLPVQD 82
>sp|Q9VAL0|SPCS1_DROME Probable signal peptidase complex subunit 1 (Microsomal signal
peptidase 12 kDa subunit) (SPase 12 kDa subunit)
Length = 98
Score = 43.9 bits (102), Expect = 1e-04
Identities = 26/87 (29%), Positives = 36/87 (41%), Gaps = 3/87 (3%)
Frame = +2
Query: 14 IPHHLDFVGQKFAEKXXXXXXXXXXXXXXXWGYYCQQISQTXXXXXXXXXXXXXXXXIPW 193
I H+DF GQ AE+ +G + QQ SQT PW
Sbjct: 4 IQTHMDFAGQGKAERWSRFIITFFGIVGLVYGAFVQQFSQTVYILGAGFVLSSLITIPPW 63
Query: 194 PFFRRYPLNWQKVSESEL---HAEAGD 265
P +RR L WQK +++ +E+GD
Sbjct: 64 PLYRRNALKWQKPIDTDAKSSSSESGD 90
>sp|Q61CQ8|SPCS1_CAEBR Probable signal peptidase complex subunit 1 (Microsomal signal
peptidase 12 kDa subunit) (SPase 12 kDa subunit)
Length = 113
Score = 41.2 bits (95), Expect = 7e-04
Identities = 24/88 (27%), Positives = 35/88 (39%), Gaps = 1/88 (1%)
Frame = +2
Query: 5 IESIPHHLDFVGQKFAEKXXXXXXXXXXXXXXXWGYYCQQISQTXXXXXXXXXXXXXXXX 184
++ + H+DF GQK AE+ GY QQ+S
Sbjct: 12 LQQLSSHIDFQGQKVAERTYQVILTLAGIIGFFVGYSTQQLSYAMYTVMGAAVFTALIIL 71
Query: 185 IPWPF-FRRYPLNWQKVSESELHAEAGD 265
PWPF FR+ P+ WQ E + + + D
Sbjct: 72 PPWPFLFRKNPIVWQTPIEEQEASSSSD 99
>sp|O44953|SPCS1_CAEEL Probable signal peptidase complex subunit 1 (Microsomal signal
peptidase 12 kDa subunit) (SPase 12 kDa subunit)
Length = 105
Score = 40.4 bits (93), Expect = 0.001
Identities = 24/88 (27%), Positives = 37/88 (42%), Gaps = 1/88 (1%)
Frame = +2
Query: 5 IESIPHHLDFVGQKFAEKXXXXXXXXXXXXXXXWGYYCQQISQTXXXXXXXXXXXXXXXX 184
++ + H+DF GQK AE+ G++ QQ+S
Sbjct: 12 LQKLSSHIDFQGQKVAERTYQVILTIAGIIGFLVGFWTQQLSYAMFTVLGASAFTALIIL 71
Query: 185 IPWPF-FRRYPLNWQKVSESELHAEAGD 265
PWPF FR+ P+ W +E + E+GD
Sbjct: 72 PPWPFLFRKNPIVWHTPAEPQ---ESGD 96
>sp|P67048|TYSY_STAAW Thymidylate synthase (TS) (TSase)
sp|P67047|TYSY_STAAN Thymidylate synthase (TS) (TSase)
sp|P67046|TYSY_STAAM Thymidylate synthase (TS) (TSase)
sp|Q6G9D4|TYSY_STAAS Thymidylate synthase (TS) (TSase)
Length = 318
Score = 30.8 bits (68), Expect = 1.0
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
Frame = +1
Query: 67 EYNSNLWNNWIYMGLLLST--NFPNFIGFGRRICS 165
+YN+N+WN W + + S N P+ FG R S
Sbjct: 79 KYNNNIWNEWAFENYIKSDEYNGPDMTDFGHRALS 113
>sp|Q5HFZ6|TYSY_STAAC Thymidylate synthase (TS) (TSase)
sp|Q6GGY0|TYSY_STAAR Thymidylate synthase (TS) (TSase)
Length = 318
Score = 30.8 bits (68), Expect = 1.0
Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
Frame = +1
Query: 67 EYNSNLWNNWIYMGLLLSTNF--PNFIGFGRRICS 165
+YN+N+WN W + + S + P+ FG R S
Sbjct: 79 KYNNNIWNEWAFENYIKSDEYKGPDMTDFGHRALS 113
>sp|P0C0M5|TYSY_STAES Thymidylate synthase (TS) (TSase)
sp|P0A0M5|TYSY_STAAU Thymidylate synthase (TS) (TSase)
sp|Q5HMP6|TYSY_STAEQ Thymidylate synthase (TS) (TSase)
Length = 318
Score = 30.0 bits (66), Expect = 1.7
Identities = 11/32 (34%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
Frame = +1
Query: 67 EYNSNLWNNWIYMGLLLSTNF--PNFIGFGRR 156
+YN+N+WN W + + S ++ P+ FG R
Sbjct: 79 KYNNNIWNEWAFENYVQSDDYHGPDMTDFGHR 110
>sp|Q944J0|SPCS1_ARATH Probable signal peptidase complex subunit 1 (Microsomal signal
peptidase 12 kDa subunit) (SPase 12 kDa subunit)
Length = 92
Score = 29.6 bits (65), Expect = 2.2
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +2
Query: 191 WPFFRRYPLNWQKVSESELH 250
WPF+ +PL W SE+E H
Sbjct: 56 WPFYNLHPLKWLDPSEAEKH 75
>sp|Q81R23|TYSY_BACAN Thymidylate synthase (TS) (TSase)
sp|Q6HJC7|TYSY_BACHK Thymidylate synthase (TS) (TSase)
Length = 318
Score = 28.9 bits (63), Expect = 3.8
Identities = 11/32 (34%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
Frame = +1
Query: 67 EYNSNLWNNWIYMGLLLSTNF--PNFIGFGRR 156
++N+N+WN W + + S + P+ I FG R
Sbjct: 78 QHNNNIWNEWAFKSWVESDEYTGPDMIDFGLR 109
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,924,010
Number of Sequences: 369166
Number of extensions: 596823
Number of successful extensions: 1164
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1161
length of database: 68,354,980
effective HSP length: 92
effective length of database: 51,359,360
effective search space used: 1694858880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)