Planaria EST Database

DrC_00875

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00875
         (737 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9GZZ9|UE1D1_HUMAN  Ubiquitin-activating enzyme E1-domain...   169   5e-42
sp|Q5R8X4|UE1D1_PONPY  Ubiquitin-activating enzyme E1-domain...   169   7e-42
sp|Q5M7A4|UE1D1_RAT  Ubiquitin-activating enzyme E1-domain c...   157   2e-38
sp|Q8VE47|UE1D1_MOUSE  Ubiquitin-activating enzyme E1-domain...   155   1e-37
sp|P46467|VPS4B_MOUSE  Vacuolar sorting protein 4b (SKD1 pro...    38   0.032
sp|O75351|VPS4B_HUMAN  Vacuolar sorting protein 4b (SKD1 pro...    37   0.042
sp|Q9UHK6|AMACR_HUMAN  Alpha-methylacyl-CoA racemase (2-meth...    34   0.46 
sp|Q03763|DSG1_BOVIN  Desmoglein-1 precursor (Desmosomal gly...    33   0.61 
sp|P52917|VPS4_YEAST  Vacuolar protein sorting-associated pr...    33   0.61 
sp|O07347|SRP54_THEAQ  Signal recognition particle protein (...    33   1.0  

>sp|Q9GZZ9|UE1D1_HUMAN Ubiquitin-activating enzyme E1-domain containing protein 1
           (UFM1-activating enzyme) (Ubiquitin-activating enzyme 5)
           (ThiFP1)
          Length = 404

 Score =  169 bits (429), Expect = 5e-42
 Identities = 89/179 (49%), Positives = 124/179 (69%), Gaps = 2/179 (1%)
 Frame = +1

Query: 187 FECLPPLIVASGLDEKTLKRDGVCAASLPTTMAIVAGFLVQNSLKFLLNFGTVSEFIGYG 366
           F C PPL+VA+ +DEKTLKR+GVCAASLPTTM +VAG LVQN LKFLLNFGTVS ++GY 
Sbjct: 227 FACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYN 286

Query: 367 ALEDNFYKVSLKPNPECGDRFCLMRQAEYREILRNTVVNCEPIEDKEEIVHEDNEFQIEL 546
           A++D F  +S+KPNP+C DR C  +Q EY++ +   +   E I+++EEI+HEDNE+ IEL
Sbjct: 287 AMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVA-ALPKQEVIQEEEEIIHEDNEWGIEL 345

Query: 547 VSDEEDAVPGDLLRKSLNLGDGVKLAY--DPNQSGKGNEAFVDKTELSLDELMKQMKNL 717
           VS+  +    +      +L +G+ +AY     Q     E  V+ +  SL++LM +MKN+
Sbjct: 346 VSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM 404

>sp|Q5R8X4|UE1D1_PONPY Ubiquitin-activating enzyme E1-domain containing protein 1
           (UFM1-activating enzyme)
          Length = 404

 Score =  169 bits (428), Expect = 7e-42
 Identities = 89/179 (49%), Positives = 124/179 (69%), Gaps = 2/179 (1%)
 Frame = +1

Query: 187 FECLPPLIVASGLDEKTLKRDGVCAASLPTTMAIVAGFLVQNSLKFLLNFGTVSEFIGYG 366
           F C PPL+VA+ +DEK+LKR+GVCAASLPTTM +VAG LVQN LKFLLNFGTVS ++GY 
Sbjct: 227 FACAPPLVVAANIDEKSLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYN 286

Query: 367 ALEDNFYKVSLKPNPECGDRFCLMRQAEYREILRNTVVNCEPIEDKEEIVHEDNEFQIEL 546
           A++D F  +S+KPNP+C DR C  +Q EY++ +   +   E I++KEEI+HEDNE+ IEL
Sbjct: 287 AMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVA-ALPKQEVIQEKEEIIHEDNEWGIEL 345

Query: 547 VSDEEDAVPGDLLRKSLNLGDGVKLAY--DPNQSGKGNEAFVDKTELSLDELMKQMKNL 717
           VS+  +    +      +L +G+ +AY     Q     E  V+ +  SL++LM +MKN+
Sbjct: 346 VSEVSEEELKNSSGPVPDLPEGITVAYTIPKKQEDSVTEVTVEDSGESLEDLMAKMKNM 404

>sp|Q5M7A4|UE1D1_RAT Ubiquitin-activating enzyme E1-domain containing protein 1
           (UFM1-activating enzyme)
          Length = 403

 Score =  157 bits (398), Expect = 2e-38
 Identities = 83/179 (46%), Positives = 117/179 (65%), Gaps = 2/179 (1%)
 Frame = +1

Query: 187 FECLPPLIVASGLDEKTLKRDGVCAASLPTTMAIVAGFLVQNSLKFLLNFGTVSEFIGYG 366
           F C PPL+VAS +DEKTLKR+GVCAASLPTTM +VAG LVQN LKFLL FGTVS ++GY 
Sbjct: 225 FACAPPLVVASNIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLKFGTVSFYLGYN 284

Query: 367 ALEDNFYKVSLKPNPECGDRFCLMRQAEYREILRNTVVNCEPIEDKEEIVHEDNEFQIEL 546
           A++D F  + +KPNP+C D+ C  +Q EY++            +++EE+VHEDNE+ IEL
Sbjct: 285 AMQDFFPTMFMKPNPQCDDKNCRKQQEEYKKRAPAQPTQETAPQEEEEVVHEDNEWGIEL 344

Query: 547 VSDEEDAVPGDLLRKSLNLGDGVKLAY--DPNQSGKGNEAFVDKTELSLDELMKQMKNL 717
           VS+  +    +       L +G+ +AY     +    +E  V+ +  SL++LM +MK +
Sbjct: 345 VSEVSEEELKNSSGPVPTLPEGITVAYTVPKKREDSVSEVTVEDSGESLEDLMARMKKM 403

>sp|Q8VE47|UE1D1_MOUSE Ubiquitin-activating enzyme E1-domain containing protein 1
           (UFM1-activating enzyme)
          Length = 403

 Score =  155 bits (392), Expect = 1e-37
 Identities = 82/179 (45%), Positives = 114/179 (63%), Gaps = 2/179 (1%)
 Frame = +1

Query: 187 FECLPPLIVASGLDEKTLKRDGVCAASLPTTMAIVAGFLVQNSLKFLLNFGTVSEFIGYG 366
           F C PPL+VAS +DEKTLKR+GVCAASLPTTM +VAG LVQN LKFLL FGTVS ++GY 
Sbjct: 225 FACAPPLVVASNIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLKFGTVSFYLGYN 284

Query: 367 ALEDNFYKVSLKPNPECGDRFCLMRQAEYREILRNTVVNCEPIEDKEEIVHEDNEFQIEL 546
           A++D F  + +KPNP C D+ C  +Q EY++            +++ E+VHEDNE+ IEL
Sbjct: 285 AMQDFFPTMFMKPNPHCDDKNCRKQQEEYKKRAAALPTQEAEPQEEAEVVHEDNEWGIEL 344

Query: 547 VSDEEDAVPGDLLRKSLNLGDGVKLAY--DPNQSGKGNEAFVDKTELSLDELMKQMKNL 717
           VS+  +    +       L +G+ +AY          +E  V+ +  SL++LM +MK +
Sbjct: 345 VSEVSEEELKNSSGPVPTLPEGITVAYTVPKKTEDSASEVTVEDSGESLEDLMARMKTM 403

>sp|P46467|VPS4B_MOUSE Vacuolar sorting protein 4b (SKD1 protein)
          Length = 444

 Score = 37.7 bits (86), Expect = 0.032
 Identities = 18/29 (62%), Positives = 22/29 (75%)
 Frame = -3

Query: 159 MKLALSRNKPTVNQKDLEKLIQFTTDFGQ 73
           M  +LS  KPTVN++DL KL +FT DFGQ
Sbjct: 414 MLRSLSSTKPTVNEQDLLKLKKFTEDFGQ 442

>sp|O75351|VPS4B_HUMAN Vacuolar sorting protein 4b (SKD1 protein)
          Length = 444

 Score = 37.4 bits (85), Expect = 0.042
 Identities = 18/29 (62%), Positives = 21/29 (72%)
 Frame = -3

Query: 159 MKLALSRNKPTVNQKDLEKLIQFTTDFGQ 73
           M  +LS  KPTVN+ DL KL +FT DFGQ
Sbjct: 414 MLRSLSNTKPTVNEHDLLKLKKFTEDFGQ 442

>sp|Q9UHK6|AMACR_HUMAN Alpha-methylacyl-CoA racemase (2-methylacyl-CoA racemase)
          Length = 382

 Score = 33.9 bits (76), Expect = 0.46
 Identities = 29/97 (29%), Positives = 43/97 (44%), Gaps = 22/97 (22%)
 Frame = +1

Query: 349 EFIGYGALEDNFYKVSLK---------PN-------PEC----GDRFCLMRQAEYREILR 468
           EF+  GA+E  FY++ +K         PN       PE      D F    +AE+ +I  
Sbjct: 229 EFMAVGAIEPQFYELLIKGLGLKSDELPNQMSMDDWPEMKKKFADVFAKKTKAEWCQIFD 288

Query: 469 NTVVNCEPIEDKEEIVHEDN--EFQIELVSDEEDAVP 573
            T     P+   EE+VH D+  E    + S+E+D  P
Sbjct: 289 GTDACVTPVLTFEEVVHHDHNKERGSFITSEEQDVSP 325

>sp|Q03763|DSG1_BOVIN Desmoglein-1 precursor (Desmosomal glycoprotein 1) (DG1) (DGI)
           (Pemphigus foliaceus antigen)
          Length = 1043

 Score = 33.5 bits (75), Expect = 0.61
 Identities = 33/122 (27%), Positives = 49/122 (40%), Gaps = 6/122 (4%)
 Frame = +1

Query: 286 IVAGFLVQNSLKFLLNFGTVSEFIGYGALEDNFYKVSLKPNPECGDRFCLMRQAEYRE-- 459
           ++ GFLV   + FLL         G GA        + +P PEC D        E  +  
Sbjct: 557 LIMGFLVLGLVPFLLMCCDCGGAPGGGA--------AFEPVPECSDGAIHSWAVEGAQAD 608

Query: 460 --ILRNTVVNCEPIEDKEEIVHEDNE--FQIELVSDEEDAVPGDLLRKSLNLGDGVKLAY 627
             +L N+ V C P+ +   I + DN   +  E  + E   + G        L DGVK++ 
Sbjct: 609 PGVLANSAVPCIPVTNANVIEYVDNSGVYTNEYGAREMQDLGGGERTTGFELTDGVKMSG 668

Query: 628 DP 633
            P
Sbjct: 669 GP 670

>sp|P52917|VPS4_YEAST Vacuolar protein sorting-associated protein VPS4 (END13 protein)
           (DOA4-independent degradation protein 6)
          Length = 437

 Score = 33.5 bits (75), Expect = 0.61
 Identities = 15/26 (57%), Positives = 18/26 (69%)
 Frame = -3

Query: 150 ALSRNKPTVNQKDLEKLIQFTTDFGQ 73
           A+   +PTVN+ DL K  QFT DFGQ
Sbjct: 409 AIKSTRPTVNEDDLLKQEQFTRDFGQ 434

>sp|O07347|SRP54_THEAQ Signal recognition particle protein (Fifty-four homolog)
          Length = 430

 Score = 32.7 bits (73), Expect = 1.0
 Identities = 20/53 (37%), Positives = 27/53 (50%)
 Frame = +1

Query: 559 EDAVPGDLLRKSLNLGDGVKLAYDPNQSGKGNEAFVDKTELSLDELMKQMKNL 717
           E   P  L  + L +GD   LA     +G   EA     ELSL++ +KQM+NL
Sbjct: 280 EPFYPERLAGRILGMGDVASLAEKVRAAGLEAEAPKSAKELSLEDFLKQMQNL 332

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,582,082
Number of Sequences: 369166
Number of extensions: 1676642
Number of successful extensions: 5827
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5452
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5809
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6631293200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00875

  1. Dr_sW_022_B05
  2. Dr_sW_006_L12