Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_B05
(721 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9GZZ9|UE1D1_HUMAN Ubiquitin-activating enzyme E1-domain... 169 5e-42
sp|Q5R8X4|UE1D1_PONPY Ubiquitin-activating enzyme E1-domain... 169 7e-42
sp|Q5M7A4|UE1D1_RAT Ubiquitin-activating enzyme E1-domain c... 157 2e-38
sp|Q8VE47|UE1D1_MOUSE Ubiquitin-activating enzyme E1-domain... 155 1e-37
sp|P46467|VPS4B_MOUSE Vacuolar sorting protein 4b (SKD1 pro... 38 0.031
sp|O75351|VPS4B_HUMAN Vacuolar sorting protein 4b (SKD1 pro... 37 0.041
sp|Q9UHK6|AMACR_HUMAN Alpha-methylacyl-CoA racemase (2-meth... 34 0.45
sp|Q03763|DSG1_BOVIN Desmoglein-1 precursor (Desmosomal gly... 33 0.59
sp|P52917|VPS4_YEAST Vacuolar protein sorting-associated pr... 33 0.59
sp|O07347|SRP54_THEAQ Signal recognition particle protein (... 33 1.0
>sp|Q9GZZ9|UE1D1_HUMAN Ubiquitin-activating enzyme E1-domain containing protein 1
(UFM1-activating enzyme) (Ubiquitin-activating enzyme 5)
(ThiFP1)
Length = 404
Score = 169 bits (429), Expect = 5e-42
Identities = 89/179 (49%), Positives = 124/179 (69%), Gaps = 2/179 (1%)
Frame = +1
Query: 187 FECLPPLIVASGLDEKTLKRDGVCAASLPTTMAIVAGFLVQNSLKFLLNFGTVSEFIGYG 366
F C PPL+VA+ +DEKTLKR+GVCAASLPTTM +VAG LVQN LKFLLNFGTVS ++GY
Sbjct: 227 FACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYN 286
Query: 367 ALEDNFYKVSLKPNPECGDRFCLMRQAEYREILRNTVVNCEPIEDKEEIVHEDNEFQIEL 546
A++D F +S+KPNP+C DR C +Q EY++ + + E I+++EEI+HEDNE+ IEL
Sbjct: 287 AMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVA-ALPKQEVIQEEEEIIHEDNEWGIEL 345
Query: 547 VSDEEDAVPGDLLRKSLNLGDGVKLAY--DPNQSGKGNEAFVDKTELSLDELMKQMKNL 717
VS+ + + +L +G+ +AY Q E V+ + SL++LM +MKN+
Sbjct: 346 VSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM 404
>sp|Q5R8X4|UE1D1_PONPY Ubiquitin-activating enzyme E1-domain containing protein 1
(UFM1-activating enzyme)
Length = 404
Score = 169 bits (428), Expect = 7e-42
Identities = 89/179 (49%), Positives = 124/179 (69%), Gaps = 2/179 (1%)
Frame = +1
Query: 187 FECLPPLIVASGLDEKTLKRDGVCAASLPTTMAIVAGFLVQNSLKFLLNFGTVSEFIGYG 366
F C PPL+VA+ +DEK+LKR+GVCAASLPTTM +VAG LVQN LKFLLNFGTVS ++GY
Sbjct: 227 FACAPPLVVAANIDEKSLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYN 286
Query: 367 ALEDNFYKVSLKPNPECGDRFCLMRQAEYREILRNTVVNCEPIEDKEEIVHEDNEFQIEL 546
A++D F +S+KPNP+C DR C +Q EY++ + + E I++KEEI+HEDNE+ IEL
Sbjct: 287 AMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVA-ALPKQEVIQEKEEIIHEDNEWGIEL 345
Query: 547 VSDEEDAVPGDLLRKSLNLGDGVKLAY--DPNQSGKGNEAFVDKTELSLDELMKQMKNL 717
VS+ + + +L +G+ +AY Q E V+ + SL++LM +MKN+
Sbjct: 346 VSEVSEEELKNSSGPVPDLPEGITVAYTIPKKQEDSVTEVTVEDSGESLEDLMAKMKNM 404
>sp|Q5M7A4|UE1D1_RAT Ubiquitin-activating enzyme E1-domain containing protein 1
(UFM1-activating enzyme)
Length = 403
Score = 157 bits (398), Expect = 2e-38
Identities = 83/179 (46%), Positives = 117/179 (65%), Gaps = 2/179 (1%)
Frame = +1
Query: 187 FECLPPLIVASGLDEKTLKRDGVCAASLPTTMAIVAGFLVQNSLKFLLNFGTVSEFIGYG 366
F C PPL+VAS +DEKTLKR+GVCAASLPTTM +VAG LVQN LKFLL FGTVS ++GY
Sbjct: 225 FACAPPLVVASNIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLKFGTVSFYLGYN 284
Query: 367 ALEDNFYKVSLKPNPECGDRFCLMRQAEYREILRNTVVNCEPIEDKEEIVHEDNEFQIEL 546
A++D F + +KPNP+C D+ C +Q EY++ +++EE+VHEDNE+ IEL
Sbjct: 285 AMQDFFPTMFMKPNPQCDDKNCRKQQEEYKKRAPAQPTQETAPQEEEEVVHEDNEWGIEL 344
Query: 547 VSDEEDAVPGDLLRKSLNLGDGVKLAY--DPNQSGKGNEAFVDKTELSLDELMKQMKNL 717
VS+ + + L +G+ +AY + +E V+ + SL++LM +MK +
Sbjct: 345 VSEVSEEELKNSSGPVPTLPEGITVAYTVPKKREDSVSEVTVEDSGESLEDLMARMKKM 403
>sp|Q8VE47|UE1D1_MOUSE Ubiquitin-activating enzyme E1-domain containing protein 1
(UFM1-activating enzyme)
Length = 403
Score = 155 bits (392), Expect = 1e-37
Identities = 82/179 (45%), Positives = 114/179 (63%), Gaps = 2/179 (1%)
Frame = +1
Query: 187 FECLPPLIVASGLDEKTLKRDGVCAASLPTTMAIVAGFLVQNSLKFLLNFGTVSEFIGYG 366
F C PPL+VAS +DEKTLKR+GVCAASLPTTM +VAG LVQN LKFLL FGTVS ++GY
Sbjct: 225 FACAPPLVVASNIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLKFGTVSFYLGYN 284
Query: 367 ALEDNFYKVSLKPNPECGDRFCLMRQAEYREILRNTVVNCEPIEDKEEIVHEDNEFQIEL 546
A++D F + +KPNP C D+ C +Q EY++ +++ E+VHEDNE+ IEL
Sbjct: 285 AMQDFFPTMFMKPNPHCDDKNCRKQQEEYKKRAAALPTQEAEPQEEAEVVHEDNEWGIEL 344
Query: 547 VSDEEDAVPGDLLRKSLNLGDGVKLAY--DPNQSGKGNEAFVDKTELSLDELMKQMKNL 717
VS+ + + L +G+ +AY +E V+ + SL++LM +MK +
Sbjct: 345 VSEVSEEELKNSSGPVPTLPEGITVAYTVPKKTEDSASEVTVEDSGESLEDLMARMKTM 403
>sp|P46467|VPS4B_MOUSE Vacuolar sorting protein 4b (SKD1 protein)
Length = 444
Score = 37.7 bits (86), Expect = 0.031
Identities = 18/29 (62%), Positives = 22/29 (75%)
Frame = -2
Query: 159 MKLALSRNKPTVNQKDLEKLIQFTTDFGQ 73
M +LS KPTVN++DL KL +FT DFGQ
Sbjct: 414 MLRSLSSTKPTVNEQDLLKLKKFTEDFGQ 442
>sp|O75351|VPS4B_HUMAN Vacuolar sorting protein 4b (SKD1 protein)
Length = 444
Score = 37.4 bits (85), Expect = 0.041
Identities = 18/29 (62%), Positives = 21/29 (72%)
Frame = -2
Query: 159 MKLALSRNKPTVNQKDLEKLIQFTTDFGQ 73
M +LS KPTVN+ DL KL +FT DFGQ
Sbjct: 414 MLRSLSNTKPTVNEHDLLKLKKFTEDFGQ 442
>sp|Q9UHK6|AMACR_HUMAN Alpha-methylacyl-CoA racemase (2-methylacyl-CoA racemase)
Length = 382
Score = 33.9 bits (76), Expect = 0.45
Identities = 29/97 (29%), Positives = 43/97 (44%), Gaps = 22/97 (22%)
Frame = +1
Query: 349 EFIGYGALEDNFYKVSLK---------PN-------PEC----GDRFCLMRQAEYREILR 468
EF+ GA+E FY++ +K PN PE D F +AE+ +I
Sbjct: 229 EFMAVGAIEPQFYELLIKGLGLKSDELPNQMSMDDWPEMKKKFADVFAKKTKAEWCQIFD 288
Query: 469 NTVVNCEPIEDKEEIVHEDN--EFQIELVSDEEDAVP 573
T P+ EE+VH D+ E + S+E+D P
Sbjct: 289 GTDACVTPVLTFEEVVHHDHNKERGSFITSEEQDVSP 325
>sp|Q03763|DSG1_BOVIN Desmoglein-1 precursor (Desmosomal glycoprotein 1) (DG1) (DGI)
(Pemphigus foliaceus antigen)
Length = 1043
Score = 33.5 bits (75), Expect = 0.59
Identities = 33/122 (27%), Positives = 49/122 (40%), Gaps = 6/122 (4%)
Frame = +1
Query: 286 IVAGFLVQNSLKFLLNFGTVSEFIGYGALEDNFYKVSLKPNPECGDRFCLMRQAEYRE-- 459
++ GFLV + FLL G GA + +P PEC D E +
Sbjct: 557 LIMGFLVLGLVPFLLMCCDCGGAPGGGA--------AFEPVPECSDGAIHSWAVEGAQAD 608
Query: 460 --ILRNTVVNCEPIEDKEEIVHEDNE--FQIELVSDEEDAVPGDLLRKSLNLGDGVKLAY 627
+L N+ V C P+ + I + DN + E + E + G L DGVK++
Sbjct: 609 PGVLANSAVPCIPVTNANVIEYVDNSGVYTNEYGAREMQDLGGGERTTGFELTDGVKMSG 668
Query: 628 DP 633
P
Sbjct: 669 GP 670
>sp|P52917|VPS4_YEAST Vacuolar protein sorting-associated protein VPS4 (END13 protein)
(DOA4-independent degradation protein 6)
Length = 437
Score = 33.5 bits (75), Expect = 0.59
Identities = 15/26 (57%), Positives = 18/26 (69%)
Frame = -2
Query: 150 ALSRNKPTVNQKDLEKLIQFTTDFGQ 73
A+ +PTVN+ DL K QFT DFGQ
Sbjct: 409 AIKSTRPTVNEDDLLKQEQFTRDFGQ 434
>sp|O07347|SRP54_THEAQ Signal recognition particle protein (Fifty-four homolog)
Length = 430
Score = 32.7 bits (73), Expect = 1.0
Identities = 20/53 (37%), Positives = 27/53 (50%)
Frame = +1
Query: 559 EDAVPGDLLRKSLNLGDGVKLAYDPNQSGKGNEAFVDKTELSLDELMKQMKNL 717
E P L + L +GD LA +G EA ELSL++ +KQM+NL
Sbjct: 280 EPFYPERLAGRILGMGDVASLAEKVRAAGLEAEAPKSAKELSLEDFLKQMQNL 332
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,338,799
Number of Sequences: 369166
Number of extensions: 1649195
Number of successful extensions: 5799
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5427
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5782
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6413660220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)