Planaria EST Database


DrC_00861

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00861
         (763 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P40492|YIJ7_YEAST  Hypothetical 59.9 kDa protein in SGA1-...    42   0.002
sp|Q96G75|U2508_HUMAN  Protein UNQ2508/PRO5996                     32   1.4  
sp|Q91YQ7|U2508_MOUSE  Protein UNQ2508/PRO5996 homolog             32   1.9  
sp|P22611|VMT8_MYXVL  Kelch repeat protein M-T8                    31   3.2  
sp|Q96TP5|ACEA_COCIM  Isocitrate lyase (Isocitrase) (Isocitr...    31   3.2  
sp|Q86ZF1|ACEA_LEPMC  Isocitrate lyase (Isocitrase) (Isocitr...    30   5.4  
sp|P29551|EF3_PNECA  Elongation factor 3 (EF-3)                    30   7.1  
sp|P04958|TETX_CLOTE  Tetanus toxin precursor (Tentoxylysin)...    30   9.3  
sp|Q12508|RMD5_YEAST  Sporulation protein RMD5 (Glucose-indu...    30   9.3  
sp|Q9PL60|Y248_CHLMU  Hypothetical protein TC0248                  30   9.3  
>sp|P40492|YIJ7_YEAST Hypothetical 59.9 kDa protein in SGA1-KTR7 intergenic region
          Length = 516

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 40/166 (24%), Positives = 57/166 (34%), Gaps = 48/166 (28%)
 Frame = +2

Query: 308 DEDWIVQRFHSFILQFYSFY--HEPSVMSLMIKTGIAVRKTENCYKPE------------ 445
           D+ W V     F+  FYS Y   +   + + +  GI+  KT +C  P             
Sbjct: 353 DQRWSVLN-DLFLSDFYSMYGISQNDPLLIYLSLGISSLKTRDCLHPSDDENGNQETETA 411

Query: 446 -----------------MKRKECVVCIDRVNKISVDIPAARHDHSILICWKTGLEMNEDN 574
                            +KRK C VC +    I+  +P A H  S L             
Sbjct: 412 TTAEKEVEDLQLFTLHSLKRKNCPVCSETFKPITQALPFAHHIQSQLF-----------E 460

Query: 575 PPIAFPNGYVYSTKAIPEL-----------------IDPTDNKVVC 661
            PI  PNG VY +K + +L                 +DP D K+ C
Sbjct: 461 NPILLPNGNVYDSKKLKKLAKTLKKQNLISLNPGQIMDPVDMKIFC 506
>sp|Q96G75|U2508_HUMAN Protein UNQ2508/PRO5996
          Length = 393

 Score = 32.3 bits (72), Expect = 1.4
 Identities = 23/106 (21%), Positives = 50/106 (47%), Gaps = 10/106 (9%)
 Frame = +2

Query: 377 SVMSLMIKTGIAVRKTENCY--------KPEMKRKECVVCIDRVNKISVDIPAARHD--H 526
           S++ L +++ ++V     C         K  +++++C    +  +++ ++I        H
Sbjct: 274 SLLGLSVESPLSVSFASGCVALPVLMNIKAVIEQRQCTGVWNHKDELPIEIELGMKCWYH 333

Query: 527 SILICWKTGLEMNEDNPPIAFPNGYVYSTKAIPELIDPTDNKVVCP 664
           S+  C     + ++ NPPI    G+V S  A+ +LI+    K+ CP
Sbjct: 334 SVFACPILRQQTSDSNPPIKLICGHVISRDALNKLIN--GGKLKCP 377
>sp|Q91YQ7|U2508_MOUSE Protein UNQ2508/PRO5996 homolog
          Length = 393

 Score = 32.0 bits (71), Expect = 1.9
 Identities = 23/106 (21%), Positives = 49/106 (46%), Gaps = 10/106 (9%)
 Frame = +2

Query: 377 SVMSLMIKTGIAVRKTENCY--------KPEMKRKECVVCIDRVNKISVDIPAARHD--H 526
           S++ L +++ ++V     C         K  +++++C       +++ ++I        H
Sbjct: 274 SLLGLSVESPLSVSFASGCVALPVLMNIKAVIEQRQCTGVWSHKDELPIEIELGMKCWYH 333

Query: 527 SILICWKTGLEMNEDNPPIAFPNGYVYSTKAIPELIDPTDNKVVCP 664
           S+  C     + ++ NPPI    G+V S  A+ +LI+    K+ CP
Sbjct: 334 SVFACPILRQQTSDSNPPIKLICGHVISRDALNKLIN--GGKLKCP 377
>sp|P22611|VMT8_MYXVL Kelch repeat protein M-T8
          Length = 515

 Score = 31.2 bits (69), Expect = 3.2
 Identities = 16/45 (35%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
 Frame = -1

Query: 208 HLRVAKRMSK-FYSLFPITFFYQQIELEDLQITFKCVFDVSLFIF 77
           H  V    SK FY+LF   F  + +++ DL+  +K VFDV  +++
Sbjct: 30  HRLVLSAYSKYFYNLFNGNFLEKNVDVIDLEADYKTVFDVIYYMY 74
>sp|Q96TP5|ACEA_COCIM Isocitrate lyase (Isocitrase) (Isocitratase) (ICL)
          Length = 538

 Score = 31.2 bits (69), Expect = 3.2
 Identities = 17/69 (24%), Positives = 35/69 (50%), Gaps = 12/69 (17%)
 Frame = +2

Query: 47  VEQWLQSIQFKY------------KKGDVKNTFESDLQIFKLYLLIKEGDWEQAIKFAHS 190
           V+QW +  +++Y            K+G++K  + S++Q  KL+ L++E    +   F + 
Sbjct: 19  VKQWWKDSRWRYTKRPFTAEQIVAKRGNLKIEYPSNVQSKKLWKLVEEKFKTKTASFTYG 78

Query: 191 FCDPKMIKE 217
             DP M+ +
Sbjct: 79  CLDPTMVTQ 87
>sp|Q86ZF1|ACEA_LEPMC Isocitrate lyase (Isocitrase) (Isocitratase) (ICL)
          Length = 537

 Score = 30.4 bits (67), Expect = 5.4
 Identities = 18/83 (21%), Positives = 39/83 (46%), Gaps = 12/83 (14%)
 Frame = +2

Query: 5   LDIVEALKQGNFHPVEQWLQSIQFKY------------KKGDVKNTFESDLQIFKLYLLI 148
           +D  +A  Q     V+QW    +++Y            K+G++K T+ S+ Q  KL+ ++
Sbjct: 4   MDAEDAQFQKEVAEVKQWWNDSRWRYTKRTFTAEEIVSKRGNLKITYPSNSQSKKLWNIV 63

Query: 149 KEGDWEQAIKFAHSFCDPKMIKE 217
           +     + + + +   DP M+ +
Sbjct: 64  EHRFKNKDVSYTYGCLDPVMVTQ 86
>sp|P29551|EF3_PNECA Elongation factor 3 (EF-3)
          Length = 1042

 Score = 30.0 bits (66), Expect = 7.1
 Identities = 15/53 (28%), Positives = 29/53 (54%), Gaps = 4/53 (7%)
 Frame = -1

Query: 256 HQSHNLAQLIHRVL-FDHLRVAK---RMSKFYSLFPITFFYQQIELEDLQITF 110
           H S  L  ++  ++ ++H ++ K    MSKF +L P    YQ I + +++ +F
Sbjct: 599 HDSKFLDNVVQAIIHYEHFKLKKYMGNMSKFITLVPSARSYQDISMSEIEFSF 651
>sp|P04958|TETX_CLOTE Tetanus toxin precursor (Tentoxylysin) [Contains: Tetanus toxin
           light chain (Tetanus toxin chain L); Tetanus toxin heavy
           chain (Tetanus toxin chain H)]
          Length = 1315

 Score = 29.6 bits (65), Expect = 9.3
 Identities = 12/37 (32%), Positives = 25/37 (67%), Gaps = 1/37 (2%)
 Frame = +3

Query: 276 NWWPPHEN-GTLMKIGLCRDFTPSFYSFIHSITSLQL 383
           N++P  +  G++M++  C ++ P+F + I +ITSL +
Sbjct: 182 NYFPCRDGFGSIMQMAFCPEYVPTFDNVIENITSLTI 218
>sp|Q12508|RMD5_YEAST Sporulation protein RMD5 (Glucose-induced degradation protein 2)
          Length = 421

 Score = 29.6 bits (65), Expect = 9.3
 Identities = 19/66 (28%), Positives = 25/66 (37%)
 Frame = +2

Query: 518 HDHSILICWKTGLEMNEDNPPIAFPNGYVYSTKAIPELIDPTDNKVVCPRSKCRCKESEL 697
           H H I IC     E   +NPP +    ++ S KA+  L         CP        S  
Sbjct: 354 HFHPIFICPVLKEETTTENPPYSLACHHIISKKALDRLSKNGTITFKCPYCPVNTSMSST 413

Query: 698 QKVYIV 715
           +KV  V
Sbjct: 414 KKVRFV 419
>sp|Q9PL60|Y248_CHLMU Hypothetical protein TC0248
          Length = 601

 Score = 29.6 bits (65), Expect = 9.3
 Identities = 21/66 (31%), Positives = 31/66 (46%), Gaps = 9/66 (13%)
 Frame = +2

Query: 476 DRVNKISVDIPAARH----DHSILICWKTG-LEMNEDNPPIAF----PNGYVYSTKAIPE 628
           D +    +D+ AA +     H +L CWK G +E++   P   F    P+  V  TK I  
Sbjct: 424 DNMEGYPIDLQAAEYLKSFAHQVLACWKNGDIELSTPIPLFGFEKIHPHPRVQYTKPICV 483

Query: 629 LIDPTD 646
           LI+  D
Sbjct: 484 LINEQD 489
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,574,021
Number of Sequences: 369166
Number of extensions: 1745628
Number of successful extensions: 4688
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4518
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4688
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7018522000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00861

  1. Dr_sW_021_O04
  2. Dr_sW_012_I15