Planaria EST Database


DrC_00826

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00826
         (845 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P13230|GRP33_ARTSA  Glycine-rich protein GRP33                 125   1e-28
sp|Q07666|SAM68_HUMAN  KH domain containing, RNA binding, si...   122   2e-27
sp|Q60749|SAM68_MOUSE  KH domain containing, RNA binding, si...   119   8e-27
sp|Q91V33|SAM68_RAT  KH domain containing, RNA binding, sign...   119   8e-27
sp|Q8UUW7|SAM68_CHICK  KH domain containing, RNA binding, si...   119   1e-26
sp|Q17339|GLD1_CAEEL  Female germline-specific tumor suppres...    85   2e-16
sp|O01367|HOW_DROME  Held out wings protein (KH-domain prote...    76   1e-13
sp|Q15637|SF01_HUMAN  Splicing factor 1 (Zinc finger protein...    47   9e-05
sp|Q64213|SF01_MOUSE  Splicing factor 1 (Zinc finger protein...    46   1e-04
sp|Q91WJ8|FUBP1_MOUSE  Far upstream element-binding protein ...    36   0.15 
>sp|P13230|GRP33_ARTSA Glycine-rich protein GRP33
          Length = 308

 Score =  125 bits (315), Expect = 1e-28
 Identities = 55/113 (48%), Positives = 89/113 (78%)
 Frame = +3

Query: 147 IDMLSNKKVKIRAKIDFPVEQYPGVNFPGKIIGPGGKTLKELQEQTETRMSVLGAGSMRN 326
           +D+ ++ KVK+ ++   PV+Q+P  NF GK++GPGG T+K+LQ++T T++S+LG GSMR+
Sbjct: 64  MDLYNDTKVKLVSRCCLPVDQFPKYNFLGKLLGPGGSTMKQLQDETMTKISILGRGSMRD 123

Query: 327 QSKEMELIQTGDPKYQHLNERMHLQVDCLALPSEAHDKISKALVEIKKALTPD 485
           ++KE EL  +GD KY HLNE++H+++  +A P+EAH +++ AL EIKK +TP+
Sbjct: 124 RNKEEELRNSGDVKYAHLNEQLHIEIISIASPAEAHARMAYALTEIKKYITPE 176
>sp|Q07666|SAM68_HUMAN KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score =  122 bits (305), Expect = 2e-27
 Identities = 65/146 (44%), Positives = 96/146 (65%), Gaps = 3/146 (2%)
 Frame = +3

Query: 147 IDMLSNKKVKIRAKIDFPVEQYPGVNFPGKIIGPGGKTLKELQEQTETRMSVLGAGSMRN 326
           +D+ S+K +K++ ++  PV+QYP  NF GKI+GP G T+K LQE+T  ++SVLG GSMR+
Sbjct: 146 LDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRD 205

Query: 327 QSKEMELIQTGDPKYQHLNERMHLQVDCLALPSEAHDKISKALVEIKKALTPD-PSEIQQ 503
           ++KE EL + GDPKY HLN  +H+ ++    P EA+  ++ A+ E+KK L PD   +I Q
Sbjct: 206 KAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQ 265

Query: 504 PPGPLSWNEISYQQSV--AGRGRGLP 575
                 + E+SY   V    RGRG+P
Sbjct: 266 E----QFLELSYLNGVPEPSRGRGVP 287
>sp|Q60749|SAM68_MOUSE KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score =  119 bits (299), Expect = 8e-27
 Identities = 67/166 (40%), Positives = 105/166 (63%), Gaps = 9/166 (5%)
 Frame = +3

Query: 102 IRMEIRRLREGVI------DTIDMLSNKKVKIRAKIDFPVEQYPGVNFPGKIIGPGGKTL 263
           + +EI ++++G        + +D+ S+K +K++ ++  PV+QYP  NF GKI+GP G T+
Sbjct: 125 LSVEIEKIQKGESKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTI 184

Query: 264 KELQEQTETRMSVLGAGSMRNQSKEMELIQTGDPKYQHLNERMHLQVDCLALPSEAHDKI 443
           K LQE+T  ++SVLG GSMR+++KE EL + GDPKY HLN  +H+ ++    P EA+  +
Sbjct: 185 KRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALM 244

Query: 444 SKALVEIKKALTPD-PSEIQQPPGPLSWNEISYQQSV--AGRGRGL 572
           + A+ E+KK L PD   +I Q      + E+SY   V    RGRG+
Sbjct: 245 AHAMEEVKKFLVPDMMDDICQE----QFLELSYLNGVPEPSRGRGV 286
>sp|Q91V33|SAM68_RAT KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score =  119 bits (299), Expect = 8e-27
 Identities = 67/166 (40%), Positives = 105/166 (63%), Gaps = 9/166 (5%)
 Frame = +3

Query: 102 IRMEIRRLREGVI------DTIDMLSNKKVKIRAKIDFPVEQYPGVNFPGKIIGPGGKTL 263
           + +EI ++++G        + +D+ S+K +K++ ++  PV+QYP  NF GKI+GP G T+
Sbjct: 125 LSVEIEKIQKGESKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTI 184

Query: 264 KELQEQTETRMSVLGAGSMRNQSKEMELIQTGDPKYQHLNERMHLQVDCLALPSEAHDKI 443
           K LQE+T  ++SVLG GSMR+++KE EL + GDPKY HLN  +H+ ++    P EA+  +
Sbjct: 185 KRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALM 244

Query: 444 SKALVEIKKALTPD-PSEIQQPPGPLSWNEISYQQSV--AGRGRGL 572
           + A+ E+KK L PD   +I Q      + E+SY   V    RGRG+
Sbjct: 245 AHAMEEVKKFLVPDMMDDICQE----QFLELSYLNGVPEPSRGRGV 286
>sp|Q8UUW7|SAM68_CHICK KH domain containing, RNA binding, signal transduction associated
           protein 1 (Src-associated in mitosis 68 kDa protein)
           (Sam68)
          Length = 433

 Score =  119 bits (298), Expect = 1e-26
 Identities = 65/146 (44%), Positives = 95/146 (65%), Gaps = 3/146 (2%)
 Frame = +3

Query: 147 IDMLSNKKVKIRAKIDFPVEQYPGVNFPGKIIGPGGKTLKELQEQTETRMSVLGAGSMRN 326
           +D+ S+K +K++ ++  PV+QYP  NF GKI+GP G T+K LQE+T  ++SVLG GSMR+
Sbjct: 129 LDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRD 188

Query: 327 QSKEMELIQTGDPKYQHLNERMHLQVDCLALPSEAHDKISKALVEIKKALTPD-PSEIQQ 503
           ++KE EL + GDPKY HLN  +H+ ++    P EA+  ++ A+ E+KK L PD   +I Q
Sbjct: 189 KAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQ 248

Query: 504 PPGPLSWNEISYQQSV--AGRGRGLP 575
                 + E+SY   V    RGRG P
Sbjct: 249 E----QFLELSYLNGVPEPTRGRGGP 270
>sp|Q17339|GLD1_CAEEL Female germline-specific tumor suppressor gld-1 (Defective in germ
           line development protein 1)
          Length = 463

 Score = 85.1 bits (209), Expect = 2e-16
 Identities = 51/153 (33%), Positives = 79/153 (51%), Gaps = 6/153 (3%)
 Frame = +3

Query: 48  EQELLTIDAVTCPNVHSQIRMEIRRLREGVIDT------IDMLSNKKVKIRAKIDFPVEQ 209
           E++ LT+      NV   +  EI R+R  +  T      +   +   + I  KI  P  +
Sbjct: 156 EKKHLTLFPHMFSNVERLLDDEIGRVRVALFQTEFPRVELPEPAGDMISITEKIYVPKNE 215

Query: 210 YPGVNFPGKIIGPGGKTLKELQEQTETRMSVLGAGSMRNQSKEMELIQTGDPKYQHLNER 389
           YP  NF G+I+GP G T K+L++ T  ++ V G GSMR++SKE      G   ++HL + 
Sbjct: 216 YPDYNFVGRILGPRGMTAKQLEQDTGCKIMVRGKGSMRDKSKES--AHRGKANWEHLEDD 273

Query: 390 MHLQVDCLALPSEAHDKISKALVEIKKALTPDP 488
           +H+ V C    +  H K+  AL ++KK L P P
Sbjct: 274 LHVLVQCEDTENRVHIKLQAALEQVKKLLIPAP 306
>sp|O01367|HOW_DROME Held out wings protein (KH-domain protein KH93F) (Putative
           RNA-binding protein) (Muscle-specific protein) (Wings
           held out protein) (Struthio protein) (Quaking-related
           93F)
          Length = 405

 Score = 75.9 bits (185), Expect = 1e-13
 Identities = 44/146 (30%), Positives = 77/146 (52%), Gaps = 13/146 (8%)
 Frame = +3

Query: 84  PNVHSQIRM----EIRRLREGVID---------TIDMLSNKKVKIRAKIDFPVEQYPGVN 224
           PNV + +      EI R+R  +           T+       V +  K+  PV ++P  N
Sbjct: 93  PNVFTHVERLLDEEIARVRASLFQINGVKKEPLTLPEPEGSVVTMNEKVYVPVREHPDFN 152

Query: 225 FPGKIIGPGGKTLKELQEQTETRMSVLGAGSMRNQSKEMELIQTGDPKYQHLNERMHLQV 404
           F G+I+GP G T K+L+++T  ++ V G GSMR++ KE      G P ++HL++ +H+ +
Sbjct: 153 FVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKED--ANRGKPNWEHLSDDLHVLI 210

Query: 405 DCLALPSEAHDKISKALVEIKKALTP 482
                 + A  K+++A+ E++K L P
Sbjct: 211 TVEDTENRATVKLAQAVAEVQKLLVP 236
>sp|Q15637|SF01_HUMAN Splicing factor 1 (Zinc finger protein 162) (Transcription factor
           ZFM1) (Zinc finger gene in MEN1 locus) (Mammalian branch
           point binding protein mBBP) (BBP)
          Length = 639

 Score = 46.6 bits (109), Expect = 9e-05
 Identities = 17/50 (34%), Positives = 33/50 (66%)
 Frame = +3

Query: 174 KIRAKIDFPVEQYPGVNFPGKIIGPGGKTLKELQEQTETRMSVLGAGSMR 323
           ++  K+  P ++YP +NF G +IGP G TLK ++++   ++ + G GS++
Sbjct: 135 RVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVK 184
>sp|Q64213|SF01_MOUSE Splicing factor 1 (Zinc finger protein 162) (Transcription factor
           ZFM1) (mZFM) (Zinc finger gene in MEN1 locus) (Mammalian
           branch point binding protein mBBP) (BBP) (CW17)
          Length = 653

 Score = 45.8 bits (107), Expect = 1e-04
 Identities = 17/49 (34%), Positives = 32/49 (65%)
 Frame = +3

Query: 174 KIRAKIDFPVEQYPGVNFPGKIIGPGGKTLKELQEQTETRMSVLGAGSM 320
           ++  K+  P ++YP +NF G +IGP G TLK ++++   ++ + G GS+
Sbjct: 135 RVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSV 183
>sp|Q91WJ8|FUBP1_MOUSE Far upstream element-binding protein 1 (FUSE-binding protein 1)
           (FBP)
          Length = 651

 Score = 35.8 bits (81), Expect = 0.15
 Identities = 21/53 (39%), Positives = 31/53 (58%), Gaps = 1/53 (1%)
 Frame = +3

Query: 231 GKIIGPGGKTLKELQEQTETRMSVLGAGSMRNQSKEMELIQTGDP-KYQHLNE 386
           G +IG GG+T+K+LQE+   +M ++  G  +N   +  L  TGDP K Q   E
Sbjct: 194 GLVIGKGGETIKQLQERAGVKMVMIQDGP-QNTGADKPLRITGDPYKVQQAKE 245
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,633,899
Number of Sequences: 369166
Number of extensions: 1687376
Number of successful extensions: 4121
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3996
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4116
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8293644780
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00826

  1. Dr_sW_022_D10
  2. Dr_sW_021_B24
  3. Dr_sW_008_P11
  4. Dr_sW_021_E13