Planaria EST Database


DrC_00740

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00740
         (538 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9Y3E5|PTH2_HUMAN  Peptidyl-tRNA hydrolase 2, mitochondri...   118   8e-27
sp|Q8R2Y8|PTH2_MOUSE  Peptidyl-tRNA hydrolase 2, mitochondri...   117   2e-26
sp|O13830|PTH2_SCHPO  Probable peptidyl-tRNA hydrolase 2 (PT...   109   4e-24
sp|O97067|PTH2_DROME  Probable peptidyl-tRNA hydrolase 2 (PT...    96   7e-20
sp|O74017|PTH_PYRHO  Peptidyl-tRNA hydrolase (PTH)                 92   6e-19
sp|Q976I0|PTH_SULTO  Peptidyl-tRNA hydrolase (PTH)                 88   1e-17
sp|Q8U0N0|PTH_PYRFU  Peptidyl-tRNA hydrolase (PTH)                 87   3e-17
sp|Q9V108|PTH_PYRAB  Peptidyl-tRNA hydrolase (PTH)                 86   4e-17
sp|Q5JDB8|PTH_PYRKO  Peptidyl-tRNA hydrolase (PTH)                 86   8e-17
sp|Q980V1|PTH_SULSO  Peptidyl-tRNA hydrolase (PTH)                 84   3e-16
>sp|Q9Y3E5|PTH2_HUMAN Peptidyl-tRNA hydrolase 2, mitochondrial precursor (PTH 2) (Bcl-2
           inhibitor of transcription 1)
          Length = 179

 Score =  118 bits (296), Expect = 8e-27
 Identities = 63/117 (53%), Positives = 75/117 (64%)
 Frame = +2

Query: 152 SKEIKLILDARXXXXXXXXXVAAQCAHAAVGCYKCGLRSAPKMINYWEICGQPKIVVKVD 331
           S E K+IL  R         VAAQC+HAAV  YK   R  P+M+  WE CGQPK+VVK  
Sbjct: 62  SGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAP 121

Query: 332 TEESLFKLQQDAFSLNLLNVLIRDKGKTQVDSGTSTVLGIGPGPASLIDKITGNLKL 502
            EE+L  L   A  L L   LI+D G+TQ+  G+ TVLGIGPGPA LIDK+TG+LKL
Sbjct: 122 DEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVLGIGPGPADLIDKVTGHLKL 178
>sp|Q8R2Y8|PTH2_MOUSE Peptidyl-tRNA hydrolase 2, mitochondrial precursor (PTH 2)
          Length = 181

 Score =  117 bits (292), Expect = 2e-26
 Identities = 59/117 (50%), Positives = 78/117 (66%)
 Frame = +2

Query: 152 SKEIKLILDARXXXXXXXXXVAAQCAHAAVGCYKCGLRSAPKMINYWEICGQPKIVVKVD 331
           S E K+IL  R         VAAQC+HAAV  YK   R +P+++  WE CGQPK+VVK  
Sbjct: 64  SGEYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAP 123

Query: 332 TEESLFKLQQDAFSLNLLNVLIRDKGKTQVDSGTSTVLGIGPGPASLIDKITGNLKL 502
            E++L +L   A +L L   LI+D G+TQ++ G+ TVLGIGPGP  LID++TG+LKL
Sbjct: 124 DEDTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVELIDEVTGHLKL 180
>sp|O13830|PTH2_SCHPO Probable peptidyl-tRNA hydrolase 2 (PTH 2)
          Length = 205

 Score =  109 bits (273), Expect = 4e-24
 Identities = 54/115 (46%), Positives = 74/115 (64%)
 Frame = +2

Query: 158 EIKLILDARXXXXXXXXXVAAQCAHAAVGCYKCGLRSAPKMINYWEICGQPKIVVKVDTE 337
           + K++L  R         +AAQCAHAA+ CYK      P ++  WE  GQ KI ++  TE
Sbjct: 90  QTKMVLVVRTDLGMTKGKIAAQCAHAALACYKIASAVDPDLVRIWENAGQAKITLQAQTE 149

Query: 338 ESLFKLQQDAFSLNLLNVLIRDKGKTQVDSGTSTVLGIGPGPASLIDKITGNLKL 502
           E+L  LQ  A SL L   +I D G+TQ+ SG++TVLGIGPGP S+I+++TG+LKL
Sbjct: 150 ETLELLQAQAMSLGLCARVIHDAGRTQIASGSATVLGIGPGPVSVINEVTGSLKL 204
>sp|O97067|PTH2_DROME Probable peptidyl-tRNA hydrolase 2 (PTH 2)
          Length = 186

 Score = 95.5 bits (236), Expect = 7e-20
 Identities = 45/113 (39%), Positives = 70/113 (61%)
 Frame = +2

Query: 164 KLILDARXXXXXXXXXVAAQCAHAAVGCYKCGLRSAPKMINYWEICGQPKIVVKVDTEES 343
           K++L  R         +AAQC H AVG Y+  +   P+++  WE CG  KI V+V++E  
Sbjct: 73  KMVLVVRNDLKMGKGKIAAQCGHGAVGAYQRAVVRTPRLLRSWENCGCAKIAVRVESEAE 132

Query: 344 LFKLQQDAFSLNLLNVLIRDKGKTQVDSGTSTVLGIGPGPASLIDKITGNLKL 502
           L  ++++A    L   LIRD G+TQ+++ + TVL +GP  A+ ID++TG+LKL
Sbjct: 133 LMAIKKEAERQQLNTCLIRDAGRTQIEANSKTVLAVGPAAAADIDRVTGHLKL 185
>sp|O74017|PTH_PYRHO Peptidyl-tRNA hydrolase (PTH)
          Length = 121

 Score = 92.4 bits (228), Expect = 6e-19
 Identities = 50/113 (44%), Positives = 66/113 (58%)
 Frame = +2

Query: 164 KLILDARXXXXXXXXXVAAQCAHAAVGCYKCGLRSAPKMINYWEICGQPKIVVKVDTEES 343
           K ++ AR         +AAQ AH AV       +   +    W   GQ K+VVKV++EE 
Sbjct: 8   KQVIVARADLKLSKGKLAAQVAHGAVTAAFEAYKKKREWFEAWFREGQKKVVVKVESEEE 67

Query: 344 LFKLQQDAFSLNLLNVLIRDKGKTQVDSGTSTVLGIGPGPASLIDKITGNLKL 502
           LFKL+ +A  L L N LIRD G T++  GT TVL +GP P  ++DK+TGNLKL
Sbjct: 68  LFKLKAEAEKLGLPNALIRDAGLTEIPPGTVTVLAVGPAPEEIVDKVTGNLKL 120
>sp|Q976I0|PTH_SULTO Peptidyl-tRNA hydrolase (PTH)
          Length = 121

 Score = 88.2 bits (217), Expect = 1e-17
 Identities = 51/117 (43%), Positives = 67/117 (57%), Gaps = 4/117 (3%)
 Frame = +2

Query: 164 KLILDARXXXXXXXXXVAAQCAHAAVGCYKCGLRSAPKMINYWEIC----GQPKIVVKVD 331
           K+++  R         +AAQ AHAAV      L S+  +   W       GQPKIVVKV 
Sbjct: 4   KMVIVVRTDLDMGKGKIAAQVAHAAVSLVLDILNSSNSLWKEWLYKWLEEGQPKIVVKVP 63

Query: 332 TEESLFKLQQDAFSLNLLNVLIRDKGKTQVDSGTSTVLGIGPGPASLIDKITGNLKL 502
           + + L K  + A  LNL   +I+D GKTQ++ GT T +GIGP P  L+DKITG+LKL
Sbjct: 64  SLDELNKRYEKAIELNLPTTIIQDAGKTQIEPGTITCIGIGPAPVELVDKITGDLKL 120
>sp|Q8U0N0|PTH_PYRFU Peptidyl-tRNA hydrolase (PTH)
          Length = 119

 Score = 86.7 bits (213), Expect = 3e-17
 Identities = 48/113 (42%), Positives = 62/113 (54%)
 Frame = +2

Query: 164 KLILDARXXXXXXXXXVAAQCAHAAVGCYKCGLRSAPKMINYWEICGQPKIVVKVDTEES 343
           K ++ AR         +A Q AH AV       +   +    W   GQ K+VVK   EE 
Sbjct: 6   KQVIVARSDLKLSKGKLAVQVAHGAVTAAFEAYKKKREWFEAWFREGQKKVVVKAQNEEE 65

Query: 344 LFKLQQDAFSLNLLNVLIRDKGKTQVDSGTSTVLGIGPGPASLIDKITGNLKL 502
           LFKL+ +A  L L   LIRD G T++  GT TVL IGPGP  ++DK+TG+LKL
Sbjct: 66  LFKLKSEAEKLGLPTALIRDAGLTEIPPGTITVLAIGPGPEEVVDKVTGHLKL 118
>sp|Q9V108|PTH_PYRAB Peptidyl-tRNA hydrolase (PTH)
          Length = 121

 Score = 86.3 bits (212), Expect = 4e-17
 Identities = 47/115 (40%), Positives = 66/115 (57%)
 Frame = +2

Query: 158 EIKLILDARXXXXXXXXXVAAQCAHAAVGCYKCGLRSAPKMINYWEICGQPKIVVKVDTE 337
           + K ++  R         +A Q AH AV       +   +    W   GQ K+VVKV++ 
Sbjct: 6   KFKQVIVVRKDLKLSKGKLAVQVAHGAVTAAFKAYKEKREWFEKWFHEGQKKVVVKVESL 65

Query: 338 ESLFKLQQDAFSLNLLNVLIRDKGKTQVDSGTSTVLGIGPGPASLIDKITGNLKL 502
           + LFKL+ +A  LNL   LI+D G T++  GT TVL IGPGP+ +IDK+TG+LKL
Sbjct: 66  DELFKLKAEAEKLNLPTALIQDAGLTEIPPGTITVLAIGPGPSEIIDKVTGHLKL 120
>sp|Q5JDB8|PTH_PYRKO Peptidyl-tRNA hydrolase (PTH)
          Length = 118

 Score = 85.5 bits (210), Expect = 8e-17
 Identities = 47/113 (41%), Positives = 62/113 (54%)
 Frame = +2

Query: 164 KLILDARXXXXXXXXXVAAQCAHAAVGCYKCGLRSAPKMINYWEICGQPKIVVKVDTEES 343
           K ++ AR          A Q AH AV       +  P+    W   GQ K+VVK +  E 
Sbjct: 5   KQVIVARKDLKLSKGKFAVQVAHGAVTAAIKAQKEKPEWFKAWFHEGQKKVVVKAENLEE 64

Query: 344 LFKLQQDAFSLNLLNVLIRDKGKTQVDSGTSTVLGIGPGPASLIDKITGNLKL 502
           LFKL+ +A  L L   LI+D G T++  GT TVL IGPGP  ++DK+TG+LKL
Sbjct: 65  LFKLKAEAEKLGLPTALIKDAGLTEIPPGTVTVLAIGPGPEEVVDKVTGHLKL 117
>sp|Q980V1|PTH_SULSO Peptidyl-tRNA hydrolase (PTH)
          Length = 120

 Score = 83.6 bits (205), Expect = 3e-16
 Identities = 48/118 (40%), Positives = 69/118 (58%), Gaps = 4/118 (3%)
 Frame = +2

Query: 161 IKLILDARXXXXXXXXXVAAQCAHAAVGCYKCGLRSA----PKMINYWEICGQPKIVVKV 328
           IK+++  R         +AAQ AHAAV      + S      + +N W   GQPKI+VKV
Sbjct: 2   IKMVIVVRSDIKMGKGKIAAQVAHAAVTLVVSIINSNNLRWKEWLNEWLHQGQPKIIVKV 61

Query: 329 DTEESLFKLQQDAFSLNLLNVLIRDKGKTQVDSGTSTVLGIGPGPASLIDKITGNLKL 502
           ++ + +    + A ++NL   +I D GKTQ++ GT T LGIGP P +L+D ITG+LKL
Sbjct: 62  NSLDEIISRAKKAETMNLPFSIIEDAGKTQLEPGTITCLGIGPAPENLVDSITGDLKL 119
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,414,344
Number of Sequences: 369166
Number of extensions: 948457
Number of successful extensions: 2292
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2230
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2281
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3699545625
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00740

  1. Dr_sW_019_E20
  2. Dr_sW_018_C06