Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= DrC_00735 (1284 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|Q9BXI6|TBC10_HUMAN TBC1 domain family member 10A (EBP50-... 263 6e-70 sp|P58802|TBC10_MOUSE TBC1 domain family member 10A (EBP50-... 263 8e-70 sp|P53258|GYP2_YEAST GTPase-activating protein GYP2 (MAC1-d... 114 5e-25 sp|O95759|TBCD8_HUMAN TBC1 domain family member 8 (Vascular... 110 9e-24 sp|Q9Z1A9|TBCD8_MOUSE TBC1 domain family member 8 (Vascular... 108 3e-23 sp|Q60949|TBCD1_MOUSE TBC1 domain family member 1 104 6e-22 sp|Q86TI0|TBCD1_HUMAN TBC1 domain family member 1 101 4e-21 sp|O97790|TBCD1_BOVIN TBC1 domain family member 1 (Lyncein) 101 5e-21 sp|Q8BYJ6|TBCD4_MOUSE TBC1 domain family member 4 (Akt subs... 97 1e-19 sp|O60343|TBCD4_HUMAN TBC1 domain family member 4 (Akt subs... 97 1e-19
>sp|Q9BXI6|TBC10_HUMAN TBC1 domain family member 10A (EBP50-PDX interactor of 64 kDa) (EPI64 protein) Length = 508 Score = 263 bits (673), Expect = 6e-70 Identities = 133/328 (40%), Positives = 197/328 (60%) Frame = +2 Query: 164 VDKFGFTGGEQYSKPADEPDNEEVKKLRRRESKWLEVLSNWEMFMSKRFDKVRNRCRKGI 343 +DKFGF G Q ++ A E EV LR+RESKWL++L+NW+ +M+K+ K+R RC+KGI Sbjct: 55 IDKFGFIVGSQGAEGALEEVPLEV--LRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGI 112 Query: 344 PEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQEGDIAVIDQIGKDVGRQYPQHEIXXXXX 523 P +R RAWQYL G + + + G++ L GD +D I +D+ RQ+P HE+ Sbjct: 113 PPSLRGRAWQYLSGGKVKLQQNPGKFDELDMSPGDPKWLDVIERDLHRQFPFHEMFVSRG 172 Query: 524 XXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEHYLPGYFD 703 LF VLKA+++ P GYC HMP E+AFW ICE YLPGY+ Sbjct: 173 GHGQQDLFRVLKAYTLYRPEEGYCQAQAPIAAVLLMHMPAEQAFWCLVQICEKYLPGYYS 232 Query: 704 SGLERVQLDAKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVL 883 LE +QLD +IL+ LL++ P A+KHL +++P+ +M +WFMC F+R P ++V+RV Sbjct: 233 EKLEAIQLDGEILFSLLQKVSPVAHKHLSRQKIDPLLYMTEWFMCAFSRTLPWSSVLRVW 292 Query: 884 DMFFCEGIKVIFRIALALVKAAAGTDDAKRAHNSTESIVAFLRNLPPEVTTPEFIVSESL 1063 DMFFCEG+K+IFR+ L L+K A G+ + +A + LR+L P++ F+V E + Sbjct: 293 DMFFCEGVKIIFRVGLVLLKHALGSPEKVKACQGQYETIERLRSLSPKIMQEAFLVQEVV 352 Query: 1064 KFANIPAAEFVYEHLKQRRSYADGSSQL 1147 + + + EHL Q R + + +L Sbjct: 353 ELP-VTERQIEREHLIQLRRWQETRGEL 379
>sp|P58802|TBC10_MOUSE TBC1 domain family member 10A (EBP50-PDX interactor of 64 kDa) (EPI64 protein) Length = 500 Score = 263 bits (672), Expect = 8e-70 Identities = 132/328 (40%), Positives = 196/328 (59%) Frame = +2 Query: 164 VDKFGFTGGEQYSKPADEPDNEEVKKLRRRESKWLEVLSNWEMFMSKRFDKVRNRCRKGI 343 +DKFGF G Q ++ A E EV LR+RESKWL++L+NW+ +M+K+ K+R RC+KGI Sbjct: 55 IDKFGFIVGSQGAEGALEEVPLEV--LRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGI 112 Query: 344 PEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQEGDIAVIDQIGKDVGRQYPQHEIXXXXX 523 P +R RAWQYL G + + + G++ L GD +D I +D+ RQ+P HE+ Sbjct: 113 PPSLRGRAWQYLSGGKVKLQQNPGKFDELDMSPGDPKWLDVIERDLHRQFPFHEMFVSRG 172 Query: 524 XXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEHYLPGYFD 703 LF VLKA+++ P GYC HMP E+AFW +CE YLPGY+ Sbjct: 173 GHGQQDLFRVLKAYTLYRPEEGYCQAQAPIAAVLLMHMPAEQAFWCLVQVCEKYLPGYYS 232 Query: 704 SGLERVQLDAKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVL 883 LE +QLD +IL+ LL++ P A+KHL +++P+ +M +WFMC F R P ++V+RV Sbjct: 233 EKLEAIQLDGEILFSLLQKVSPVAHKHLSRQKIDPLLYMTEWFMCAFARTLPWSSVLRVW 292 Query: 884 DMFFCEGIKVIFRIALALVKAAAGTDDAKRAHNSTESIVAFLRNLPPEVTTPEFIVSESL 1063 DMFFCEG+K+IFR+ L L+K A G+ + +A + LR+L P++ F+V E + Sbjct: 293 DMFFCEGVKIIFRVGLVLLKHALGSPEKLKACQGQYETIEQLRSLSPKIMQEAFLVQEVI 352 Query: 1064 KFANIPAAEFVYEHLKQRRSYADGSSQL 1147 + + + EHL Q R + + +L Sbjct: 353 ELP-VTERQIEREHLIQLRRWQETRGEL 379
>sp|P53258|GYP2_YEAST GTPase-activating protein GYP2 (MAC1-dependent regulator) (MIC1 protein) Length = 950 Score = 114 bits (286), Expect = 5e-25 Identities = 73/278 (26%), Positives = 130/278 (46%), Gaps = 2/278 (0%) Frame = +2 Query: 149 EQEIFVDKFGFTGGEQYSKPADEPDNEEVKKLRRRESKWLEVLSNWEMFMSKRFDKVRNR 328 +++I V + G G+ + P + +E KLR + E N + + F K+ Sbjct: 186 KKDITVPRAGL--GQHFKYPGNPTMVKEKAKLRLWFDYFRENGRNLAVVQTPMFRKL--- 240 Query: 329 CRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQEGDIA--VIDQIGKDVGRQYPQH 502 R G+P +R W+ G+ + G+Y ++ + ID+I KD+ R P++ Sbjct: 241 IRIGVPNRMRGEIWELCSGAMYMRYANSGEYERILNENAGKTSQAIDEIEKDLKRSLPEY 300 Query: 503 EIXXXXXXXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEH 682 L NVL A+S P GYC M EE+AFW C +C+ Sbjct: 301 SAYQTEEGIQR--LRNVLTAYSWKNPDVGYCQAMNIVVAGFLIFMSEEQAFWCLCNLCDI 358 Query: 683 YLPGYFDSGLERVQLDAKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP* 862 Y+PGY+ + LD ++ ++ +P ++++ H+++ + WF+ +F + P Sbjct: 359 YVPGYYSKTMYGTLLDQRVFESFVEDRMPVLWEYILQHDIQLSVVSLPWFLSLFFTSMPL 418 Query: 863 ATVVRVLDMFFCEGIKVIFRIALALVKAAAGTDDAKRA 976 VR++D+FF G +F++ALA++K A DD +A Sbjct: 419 EYAVRIMDIFFMNGSITLFQVALAVLKINA--DDILQA 454
>sp|O95759|TBCD8_HUMAN TBC1 domain family member 8 (Vascular Rab-GAP/TBC-containing protein) (AD 3) Length = 897 Score = 110 bits (275), Expect = 9e-24 Identities = 67/219 (30%), Positives = 108/219 (49%), Gaps = 3/219 (1%) Frame = +2 Query: 305 RFDKVRNRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQE-GDIAVI-DQIGKD 478 R +K+R GIPE +R R W + ++ G Y L+++ G ++ ++I +D Sbjct: 251 RTEKIRKLVAMGIPESLRGRLWLLFSDAVTDLASHPGYYGNLVEESLGKCCLVTEEIERD 310 Query: 479 VGRQYPQHEIXXXXXXXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFW 658 + R P+H L VL A++ P GYC + EEEAFW Sbjct: 311 LHRSLPEHPAFQNETGIAA--LRRVLTAYAHRNPKIGYCQSMNILTSVLLLYTKEEEAFW 368 Query: 659 TFCAICEHYLPGYFDSGLERVQLDAKILYGLLKRHVPQAYKHLK-LHEVEPVYFMIDWFM 835 A+CE LP YF+ + Q+D + L+K H+P+ +H+ L + V + WF+ Sbjct: 369 LLVAVCERMLPDYFNHRVIGAQVDQSVFEELIKGHLPELAEHMNDLSALASV--SLSWFL 426 Query: 836 CIFTRNFP*ATVVRVLDMFFCEGIKVIFRIALALVKAAA 952 +F P + V V+D FF +GIK IF++ LA+++A A Sbjct: 427 TLFLSIMPLESAVNVVDCFFYDGIKAIFQLGLAVLEANA 465
>sp|Q9Z1A9|TBCD8_MOUSE TBC1 domain family member 8 (Vascular Rab-GAP/TBC-containing protein) (BUB2-like protein 1) Length = 891 Score = 108 bits (270), Expect = 3e-23 Identities = 66/219 (30%), Positives = 105/219 (47%), Gaps = 3/219 (1%) Frame = +2 Query: 305 RFDKVRNRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQEGD--IAVIDQIGKD 478 R +K+R GIPE +R R W + ++ G Y L++Q V ++I +D Sbjct: 250 RTEKIRKLVAMGIPESLRGRLWLLFSDAVTDLASHPGYYGNLVEQSLGRCCLVTEEIERD 309 Query: 479 VGRQYPQHEIXXXXXXXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFW 658 + R P+H L VL A++ P GYC + EEEAFW Sbjct: 310 LHRSLPEHPAFQNETGIAA--LRRVLTAYAHRNPKIGYCQSMNILTSVLLLYAKEEEAFW 367 Query: 659 TFCAICEHYLPGYFDSGLERVQLDAKILYGLLKRHVPQAYKHLK-LHEVEPVYFMIDWFM 835 A+CE LP YF+ + Q+D + L+K +P+ +H+ L + + + WF+ Sbjct: 368 LLVAVCERMLPDYFNHRVIGAQVDQSVFEELIKEQLPELAEHMSDLSALASI--SLSWFL 425 Query: 836 CIFTRNFP*ATVVRVLDMFFCEGIKVIFRIALALVKAAA 952 +F P + V V+D FF +GIK IF++ LA+++A A Sbjct: 426 TLFLSIMPLESAVHVVDCFFYDGIKAIFQLGLAVLEANA 464
>sp|Q60949|TBCD1_MOUSE TBC1 domain family member 1 Length = 1255 Score = 104 bits (259), Expect = 6e-22 Identities = 81/280 (28%), Positives = 121/280 (43%), Gaps = 26/280 (9%) Frame = +2 Query: 269 EVLSNWEMFMSK------RFD--KVRNRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYH 424 EV + WE +S +FD KV + +G+P R W++L F K + Sbjct: 856 EVTTVWEKMLSTPGRSKIKFDMEKVHSAVGQGVPRHHRGEIWKFLAEQ----FHLKHPFP 911 Query: 425 ALIQQEGDIAVIDQIGK----------DVGRQYPQHEIXXXXXXXXXXXLFNVLKAFSVL 574 + QQ D+ + + K D+GR +P H L+N+LKA+S+L Sbjct: 912 SK-QQPKDVPYKELLKKLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLL 970 Query: 575 YPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEHYLPGYFDSGLER--------VQLD 730 GYC HM EEEAF + FD GL + +Q+ Sbjct: 971 DQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFLM-------FDMGLRKQYRPDMIILQIQ 1023 Query: 731 AKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVLDMFFCEGIK 910 L LL + Y HL+ HE+ P + WF+ +F FP V RV DM F +G + Sbjct: 1024 MYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTVFASQFPLGFVARVFDMIFLQGSE 1083 Query: 911 VIFRIALALVKAAAGTDDAKRAHNSTESIVAFLRNLPPEV 1030 VIF++AL+L+ H + E+IV F++N P + Sbjct: 1084 VIFKVALSLL---GSHKPLILQHENLETIVDFIKNTLPNL 1120
>sp|Q86TI0|TBCD1_HUMAN TBC1 domain family member 1 Length = 1168 Score = 101 bits (252), Expect = 4e-21 Identities = 79/280 (28%), Positives = 122/280 (43%), Gaps = 26/280 (9%) Frame = +2 Query: 269 EVLSNWEMFMSK------RFD--KVRNRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYH 424 EV + WE +S +FD K+ + +G+P R W++L F K Q+ Sbjct: 769 EVTTVWEKMLSTPGRSKIKFDMEKMHSAVGQGVPRHHRGEIWKFLAEQ----FHLKHQFP 824 Query: 425 ALIQQEGDIAVIDQIGK----------DVGRQYPQHEIXXXXXXXXXXXLFNVLKAFSVL 574 + QQ D+ + + + D+GR +P H L+N+LKA+S+L Sbjct: 825 SK-QQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLL 883 Query: 575 YPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEHYLPGYFDSGLER--------VQLD 730 GYC HM EEEAF + FD GL + +Q+ Sbjct: 884 DQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFLM-------FDMGLRKQYRPDMIILQIQ 936 Query: 731 AKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVLDMFFCEGIK 910 L LL + Y HL+ HE+ P + WF+ +F FP V RV DM F +G + Sbjct: 937 MYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTE 996 Query: 911 VIFRIALALVKAAAGTDDAKRAHNSTESIVAFLRNLPPEV 1030 VIF++AL+L+ H + E+IV F+++ P + Sbjct: 997 VIFKVALSLL---GSHKPLILQHENLETIVDFIKSTLPNL 1033
>sp|O97790|TBCD1_BOVIN TBC1 domain family member 1 (Lyncein) Length = 556 Score = 101 bits (251), Expect = 5e-21 Identities = 78/281 (27%), Positives = 119/281 (42%), Gaps = 27/281 (9%) Frame = +2 Query: 269 EVLSNWEMFMSK------RFD--KVRNRCRKGIPEGIRPRAWQYL-----------CGSR 391 EV + WE +S +FD K+ + +G+P R W++L C + Sbjct: 157 EVTTVWEKILSTPGRSKIKFDMEKMHSAVGQGVPRHHRGEIWKFLAEQYHLKHPFPCKQQ 216 Query: 392 QNIFKFKGQYHALIQQEGDIAVIDQIGKDVGRQYPQHEIXXXXXXXXXXXLFNVLKAFSV 571 +K L Q+ I + D+GR +P H L+N+LKA+S+ Sbjct: 217 PKDTPYKELLKQLTSQQHAILI------DLGRTFPTHPYYSAQLGAGQLSLYNILKAYSL 270 Query: 572 LYPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEHYLPGYFDSGLER--------VQL 727 L GYC HM EEEAF + FD GL + +Q+ Sbjct: 271 LDQEVGYCQGLSFVAGILLLHMGEEEAFNMLKFLM-------FDMGLRKQYRPDMIILQI 323 Query: 728 DAKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVLDMFFCEGI 907 L LL + Y HL+ HE+ P + WF+ +F FP V RV DM F +G Sbjct: 324 QMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTVFASQFPLGFVARVFDMIFLQGS 383 Query: 908 KVIFRIALALVKAAAGTDDAKRAHNSTESIVAFLRNLPPEV 1030 +VIF++AL+L+ H + E+IV F+++ P + Sbjct: 384 EVIFKVALSLL---GSHKPLILQHENLETIVDFIKSTLPNL 421
>sp|Q8BYJ6|TBCD4_MOUSE TBC1 domain family member 4 (Akt substrate of 160 kDa) (AS160) Length = 1307 Score = 97.1 bits (240), Expect = 1e-19 Identities = 72/273 (26%), Positives = 119/273 (43%), Gaps = 13/273 (4%) Frame = +2 Query: 263 WLEVLSNWEMFMSKRFDKVRNRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQ-------Y 421 W + L N + + + ++G+P+ R WQ+L + + + Y Sbjct: 902 WDKKLLNCRTKIRCDMEDIHTSLKEGVPKSRRGEIWQFLALQYRLRHRLPNKHQPPDTSY 961 Query: 422 HALIQQEGDIAVIDQIGKDVGRQYPQHEIXXXXXXXXXXXLFNVLKAFSVLYPATGYCXX 601 L++Q A I D+GR +P H LFN+LKA+S+L GYC Sbjct: 962 KELLKQL--TAQQHAILVDLGRTFPTHPYFSVQLGAGQLSLFNLLKAYSLLDKEVGYCQG 1019 Query: 602 XXXXXXXXXXHMPEEEAFWTFCAIC------EHYLPGYFDSGLERVQLDAKILYGLLKRH 763 HM EE+AF + + Y P ++ QL LL + Sbjct: 1020 ISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSR-----LLHDY 1074 Query: 764 VPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVLDMFFCEGIKVIFRIALALVK 943 + Y HL+ +E+ P + WF+ +F FP V RV D+ F +G +VIF++AL+L+ Sbjct: 1075 HRELYNHLEENEISPSLYAAPWFLTLFASQFPLGFVARVFDIIFLQGTEVIFKVALSLL- 1133 Query: 944 AAAGTDDAKRAHNSTESIVAFLRNLPPEVTTPE 1042 + + + E+IV FL++ P++ T E Sbjct: 1134 --SSQEALIMECENFENIVEFLKSTLPDMNTTE 1164
>sp|O60343|TBCD4_HUMAN TBC1 domain family member 4 (Akt substrate of 160 kDa) (AS160) Length = 1298 Score = 96.7 bits (239), Expect = 1e-19 Identities = 75/281 (26%), Positives = 119/281 (42%), Gaps = 23/281 (8%) Frame = +2 Query: 269 EVLSNWEMFMSKRFDKVR-------NRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYHA 427 EVL W+ + K+R ++G+P+ R WQ+L + + + Sbjct: 888 EVLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNK--- 944 Query: 428 LIQQEGDIAVIDQIGK----------DVGRQYPQHEIXXXXXXXXXXXLFNVLKAFSVLY 577 QQ DI+ + + + D+GR +P H LFN+LKA+S+L Sbjct: 945 --QQPPDISYKELLKQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLD 1002 Query: 578 PATGYCXXXXXXXXXXXXHMPEEEAFWTF------CAICEHYLPGYFDSGLERVQLDAKI 739 GYC HM EE+AF + Y P ++ QL Sbjct: 1003 KEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSR-- 1060 Query: 740 LYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVLDMFFCEGIKVIF 919 LL + Y HL+ +E+ P + WF+ +F F V RV D+ F +G +VIF Sbjct: 1061 ---LLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIF 1117 Query: 920 RIALALVKAAAGTDDAKRAHNSTESIVAFLRNLPPEVTTPE 1042 ++AL+L+ + + S E+IV FL+N P++ T E Sbjct: 1118 KVALSLL---SSQETLIMECESFENIVEFLKNTLPDMNTSE 1155
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 142,477,268 Number of Sequences: 369166 Number of extensions: 2959320 Number of successful extensions: 8231 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 7746 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 8191 length of database: 68,354,980 effective HSP length: 113 effective length of database: 47,479,925 effective search space used: 14908696450 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)