Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_C16
(898 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9BXI6|TBC10_HUMAN TBC1 domain family member 10A (EBP50-... 222 1e-57
sp|P58802|TBC10_MOUSE TBC1 domain family member 10A (EBP50-... 221 2e-57
sp|P53258|GYP2_YEAST GTPase-activating protein GYP2 (MAC1-d... 102 2e-21
sp|O95759|TBCD8_HUMAN TBC1 domain family member 8 (Vascular... 93 9e-19
sp|Q9Z1A9|TBCD8_MOUSE TBC1 domain family member 8 (Vascular... 91 3e-18
sp|Q80XC3|US6NL_MOUSE USP6 N-terminal-like protein 87 6e-17
sp|Q92738|US6NL_HUMAN USP6 N-terminal-like protein (Related... 85 3e-16
sp|P48566|GYP3_YEAST GTPase-activating protein GYP3 (MSB3 p... 84 5e-16
sp|Q60949|TBCD1_MOUSE TBC1 domain family member 1 84 7e-16
sp|Q86TI0|TBCD1_HUMAN TBC1 domain family member 1 82 2e-15
>sp|Q9BXI6|TBC10_HUMAN TBC1 domain family member 10A (EBP50-PDX interactor of 64 kDa)
(EPI64 protein)
Length = 508
Score = 222 bits (566), Expect = 1e-57
Identities = 108/245 (44%), Positives = 153/245 (62%)
Frame = +2
Query: 164 VDKFGFTGGEQYSKPADEPDNEEVKKLRRRESKWLEVLSNWEMFMSKRFDKVRNRCRKGI 343
+DKFGF G Q ++ A E EV LR+RESKWL++L+NW+ +M+K+ K+R RC+KGI
Sbjct: 55 IDKFGFIVGSQGAEGALEEVPLEV--LRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGI 112
Query: 344 PEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQEGDIAVIDQIGKDVGRQYPQHEIXXXXX 523
P +R RAWQYL G + + + G++ L GD +D I +D+ RQ+P HE+
Sbjct: 113 PPSLRGRAWQYLSGGKVKLQQNPGKFDELDMSPGDPKWLDVIERDLHRQFPFHEMFVSRG 172
Query: 524 XXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEHYLPGYFD 703
LF VLKA+++ P GYC HMP E+AFW ICE YLPGY+
Sbjct: 173 GHGQQDLFRVLKAYTLYRPEEGYCQAQAPIAAVLLMHMPAEQAFWCLVQICEKYLPGYYS 232
Query: 704 SGLERVQLDAKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVL 883
LE +QLD +IL+ LL++ P A+KHL +++P+ +M +WFMC F+R P ++V+RV
Sbjct: 233 EKLEAIQLDGEILFSLLQKVSPVAHKHLSRQKIDPLLYMTEWFMCAFSRTLPWSSVLRVW 292
Query: 884 DMFFC 898
DMFFC
Sbjct: 293 DMFFC 297
>sp|P58802|TBC10_MOUSE TBC1 domain family member 10A (EBP50-PDX interactor of 64 kDa)
(EPI64 protein)
Length = 500
Score = 221 bits (564), Expect = 2e-57
Identities = 107/245 (43%), Positives = 152/245 (62%)
Frame = +2
Query: 164 VDKFGFTGGEQYSKPADEPDNEEVKKLRRRESKWLEVLSNWEMFMSKRFDKVRNRCRKGI 343
+DKFGF G Q ++ A E EV LR+RESKWL++L+NW+ +M+K+ K+R RC+KGI
Sbjct: 55 IDKFGFIVGSQGAEGALEEVPLEV--LRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGI 112
Query: 344 PEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQEGDIAVIDQIGKDVGRQYPQHEIXXXXX 523
P +R RAWQYL G + + + G++ L GD +D I +D+ RQ+P HE+
Sbjct: 113 PPSLRGRAWQYLSGGKVKLQQNPGKFDELDMSPGDPKWLDVIERDLHRQFPFHEMFVSRG 172
Query: 524 XXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEHYLPGYFD 703
LF VLKA+++ P GYC HMP E+AFW +CE YLPGY+
Sbjct: 173 GHGQQDLFRVLKAYTLYRPEEGYCQAQAPIAAVLLMHMPAEQAFWCLVQVCEKYLPGYYS 232
Query: 704 SGLERVQLDAKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVL 883
LE +QLD +IL+ LL++ P A+KHL +++P+ +M +WFMC F R P ++V+RV
Sbjct: 233 EKLEAIQLDGEILFSLLQKVSPVAHKHLSRQKIDPLLYMTEWFMCAFARTLPWSSVLRVW 292
Query: 884 DMFFC 898
DMFFC
Sbjct: 293 DMFFC 297
>sp|P53258|GYP2_YEAST GTPase-activating protein GYP2 (MAC1-dependent regulator) (MIC1
protein)
Length = 950
Score = 102 bits (253), Expect = 2e-21
Identities = 63/251 (25%), Positives = 114/251 (45%), Gaps = 2/251 (0%)
Frame = +2
Query: 149 EQEIFVDKFGFTGGEQYSKPADEPDNEEVKKLRRRESKWLEVLSNWEMFMSKRFDKVRNR 328
+++I V + G G+ + P + +E KLR + E N + + F K+
Sbjct: 186 KKDITVPRAGL--GQHFKYPGNPTMVKEKAKLRLWFDYFRENGRNLAVVQTPMFRKL--- 240
Query: 329 CRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQEGDIA--VIDQIGKDVGRQYPQH 502
R G+P +R W+ G+ + G+Y ++ + ID+I KD+ R P++
Sbjct: 241 IRIGVPNRMRGEIWELCSGAMYMRYANSGEYERILNENAGKTSQAIDEIEKDLKRSLPEY 300
Query: 503 EIXXXXXXXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEH 682
L NVL A+S P GYC M EE+AFW C +C+
Sbjct: 301 SAYQTEEGIQR--LRNVLTAYSWKNPDVGYCQAMNIVVAGFLIFMSEEQAFWCLCNLCDI 358
Query: 683 YLPGYFDSGLERVQLDAKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP* 862
Y+PGY+ + LD ++ ++ +P ++++ H+++ + WF+ +F + P
Sbjct: 359 YVPGYYSKTMYGTLLDQRVFESFVEDRMPVLWEYILQHDIQLSVVSLPWFLSLFFTSMPL 418
Query: 863 ATVVRVLDMFF 895
VR++D+FF
Sbjct: 419 EYAVRIMDIFF 429
>sp|O95759|TBCD8_HUMAN TBC1 domain family member 8 (Vascular Rab-GAP/TBC-containing
protein) (AD 3)
Length = 897
Score = 93.2 bits (230), Expect = 9e-19
Identities = 58/200 (29%), Positives = 93/200 (46%), Gaps = 3/200 (1%)
Frame = +2
Query: 305 RFDKVRNRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQE-GDIAVI-DQIGKD 478
R +K+R GIPE +R R W + ++ G Y L+++ G ++ ++I +D
Sbjct: 251 RTEKIRKLVAMGIPESLRGRLWLLFSDAVTDLASHPGYYGNLVEESLGKCCLVTEEIERD 310
Query: 479 VGRQYPQHEIXXXXXXXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFW 658
+ R P+H L VL A++ P GYC + EEEAFW
Sbjct: 311 LHRSLPEHPAFQNETGIAA--LRRVLTAYAHRNPKIGYCQSMNILTSVLLLYTKEEEAFW 368
Query: 659 TFCAICEHYLPGYFDSGLERVQLDAKILYGLLKRHVPQAYKHLK-LHEVEPVYFMIDWFM 835
A+CE LP YF+ + Q+D + L+K H+P+ +H+ L + V + WF+
Sbjct: 369 LLVAVCERMLPDYFNHRVIGAQVDQSVFEELIKGHLPELAEHMNDLSALASV--SLSWFL 426
Query: 836 CIFTRNFP*ATVVRVLDMFF 895
+F P + V V+D FF
Sbjct: 427 TLFLSIMPLESAVNVVDCFF 446
>sp|Q9Z1A9|TBCD8_MOUSE TBC1 domain family member 8 (Vascular Rab-GAP/TBC-containing
protein) (BUB2-like protein 1)
Length = 891
Score = 91.3 bits (225), Expect = 3e-18
Identities = 57/200 (28%), Positives = 90/200 (45%), Gaps = 3/200 (1%)
Frame = +2
Query: 305 RFDKVRNRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQEGD--IAVIDQIGKD 478
R +K+R GIPE +R R W + ++ G Y L++Q V ++I +D
Sbjct: 250 RTEKIRKLVAMGIPESLRGRLWLLFSDAVTDLASHPGYYGNLVEQSLGRCCLVTEEIERD 309
Query: 479 VGRQYPQHEIXXXXXXXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFW 658
+ R P+H L VL A++ P GYC + EEEAFW
Sbjct: 310 LHRSLPEHPAFQNETGIAA--LRRVLTAYAHRNPKIGYCQSMNILTSVLLLYAKEEEAFW 367
Query: 659 TFCAICEHYLPGYFDSGLERVQLDAKILYGLLKRHVPQAYKHLK-LHEVEPVYFMIDWFM 835
A+CE LP YF+ + Q+D + L+K +P+ +H+ L + + + WF+
Sbjct: 368 LLVAVCERMLPDYFNHRVIGAQVDQSVFEELIKEQLPELAEHMSDLSALASI--SLSWFL 425
Query: 836 CIFTRNFP*ATVVRVLDMFF 895
+F P + V V+D FF
Sbjct: 426 TLFLSIMPLESAVHVVDCFF 445
>sp|Q80XC3|US6NL_MOUSE USP6 N-terminal-like protein
Length = 819
Score = 87.0 bits (214), Expect = 6e-17
Identities = 65/249 (26%), Positives = 107/249 (42%), Gaps = 7/249 (2%)
Frame = +2
Query: 167 DKFGFTGGEQ---YSKPADEPDNEEVKKLRRRESKWLEVLSNWEMFMSKRFDKVRNRCRK 337
D+FGF E+ ++ AD E++ R SKWL++L WE + K +K R K
Sbjct: 46 DRFGFLHEEELPYHNAAADRQKQLEIE----RTSKWLKMLKKWERY--KNTEKFHRRIYK 99
Query: 338 GIPEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQ-EGDIAVIDQIGKDVGRQYPQHEIXX 514
GIP +R W L + + + Y L + G I QI DV R + H +
Sbjct: 100 GIPLQLRGEVWALLLEIPKMKEETRDLYSKLKHRARGCSPDIRQIDLDVNRTFRDHIMFR 159
Query: 515 XXXXXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAIC---EHY 685
LF+VL A+S+ GYC +M EE+AFW + +H
Sbjct: 160 DRYGVKQQSLFHVLAAYSIYNTEVGYCQGMSQITALLLMYMNEEDAFWALVKLFSGPKHA 219
Query: 686 LPGYFDSGLERVQLDAKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*A 865
+ G+F G ++ + +L + + + +HL E+ ++ + WF F P
Sbjct: 220 MHGFFVQGFPKLLRFQEHHEKILNKFLSKLKQHLDSQEIYTSFYTMKWFFQCFLDRTPFR 279
Query: 866 TVVRVLDMF 892
+R+ D++
Sbjct: 280 LNLRIWDIY 288
>sp|Q92738|US6NL_HUMAN USP6 N-terminal-like protein (Related to the N terminus of tre)
(RN-tre)
Length = 828
Score = 84.7 bits (208), Expect = 3e-16
Identities = 65/247 (26%), Positives = 104/247 (42%), Gaps = 5/247 (2%)
Frame = +2
Query: 167 DKFGFTGGEQYSKPADEPDNEEVKKLR-RRESKWLEVLSNWEMFMSKRFDKVRNRCRKGI 343
D+FGF E+ P E K L R +KWL++L WE + K +K R KGI
Sbjct: 46 DRFGFLHEEEL--PDHNVAVERQKHLEIERTTKWLKMLKGWEKY--KNTEKFHRRIYKGI 101
Query: 344 PEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQ-EGDIAVIDQIGKDVGRQYPQHEIXXXX 520
P +R W L + + + Y L + G I QI DV R + H +
Sbjct: 102 PLQLRGEVWALLLEIPKMKEETRDLYSKLKHRARGCSPDIRQIDLDVNRTFRDHIMFRDR 161
Query: 521 XXXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAIC---EHYLP 691
LF+VL A+S+ GYC +M EE+AFW + +H +
Sbjct: 162 YGVKQQSLFHVLAAYSIYNTEVGYCQGMSQITALLLMYMNEEDAFWALVKLFSGPKHAMH 221
Query: 692 GYFDSGLERVQLDAKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATV 871
G+F G ++ + +L + + + +HL E+ ++ + WF F P
Sbjct: 222 GFFVQGFPKLLRFQEHHEKILNKFLSKLKQHLDSQEIYTSFYTMKWFFQCFLDRTPFTLN 281
Query: 872 VRVLDMF 892
+R+ D++
Sbjct: 282 LRIWDIY 288
>sp|P48566|GYP3_YEAST GTPase-activating protein GYP3 (MSB3 protein) (Multicopy suppressor
of bud emergence 3)
Length = 633
Score = 84.0 bits (206), Expect = 5e-16
Identities = 72/298 (24%), Positives = 113/298 (37%), Gaps = 55/298 (18%)
Frame = +2
Query: 167 DKFGFTGGEQYSKPA--DEPDNEEVKKLRRRESKW--------LEVLSNWEMFMSKRFDK 316
D++GF Y A D+ E + RR+ KW L V + + ++
Sbjct: 156 DRYGFKKQNNYISEAEYDKWWVEYSQYCVRRKHKWQLLLEKSGLPVTDDSPSRFPSKSER 215
Query: 317 VRNRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYHALIQQ----------EGDIAVIDQ 466
++ RKGIP R AW + ++ + K KG Y L+++ E + +D
Sbjct: 216 LKRYVRKGIPAEWRGNAWWHFARGQEKLNKNKGVYSQLLRKMKQIKKQNPNEKQVQDLDI 275
Query: 467 IGKDVGRQYP-----QHEIXXXXXXXXXXXLFNVLKAFSVLYPATGYCXXXXXXXXXXXX 631
I +D+ R +P Q + L VL AFS+ P GYC
Sbjct: 276 IERDLNRTFPDNIHFQSSLHNKEGPPIIKSLRRVLVAFSLYNPKIGYCQSMNFLAGLLLL 335
Query: 632 HMPEEEAFWTFCAICEHYLPGYFDSGLERVQLDAKILYGLLKRHVPQAYKHLK------- 790
+ EE AFW I YLPG + LE V +D +L +K ++P+ + ++K
Sbjct: 336 FLDEERAFWMLVIITSRYLPGVHNINLEGVNIDQGVLMLCVKEYIPEVWSYIKPSIDHHQ 395
Query: 791 ----------------------LHEVEPVYF-MIDWFMCIFTRNFP*ATVVRVLDMFF 895
L+ + P+ WFM F P T +R+ D F
Sbjct: 396 KNNKTFSPSNKKVLFNMQKNEFLYRLPPITLCTASWFMSCFVGVVPIETTLRIWDCLF 453
>sp|Q60949|TBCD1_MOUSE TBC1 domain family member 1
Length = 1255
Score = 83.6 bits (205), Expect = 7e-16
Identities = 67/235 (28%), Positives = 96/235 (40%), Gaps = 26/235 (11%)
Frame = +2
Query: 269 EVLSNWEMFMSK------RFD--KVRNRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYH 424
EV + WE +S +FD KV + +G+P R W++L F K +
Sbjct: 856 EVTTVWEKMLSTPGRSKIKFDMEKVHSAVGQGVPRHHRGEIWKFLAEQ----FHLKHPFP 911
Query: 425 ALIQQEGDIAVIDQIGK----------DVGRQYPQHEIXXXXXXXXXXXLFNVLKAFSVL 574
+ QQ D+ + + K D+GR +P H L+N+LKA+S+L
Sbjct: 912 SK-QQPKDVPYKELLKKLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLL 970
Query: 575 YPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEHYLPGYFDSGLER--------VQLD 730
GYC HM EEEAF + FD GL + +Q+
Sbjct: 971 DQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFL-------MFDMGLRKQYRPDMIILQIQ 1023
Query: 731 AKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVLDMFF 895
L LL + Y HL+ HE+ P + WF+ +F FP V RV DM F
Sbjct: 1024 MYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTVFASQFPLGFVARVFDMIF 1078
>sp|Q86TI0|TBCD1_HUMAN TBC1 domain family member 1
Length = 1168
Score = 82.4 bits (202), Expect = 2e-15
Identities = 66/235 (28%), Positives = 97/235 (41%), Gaps = 26/235 (11%)
Frame = +2
Query: 269 EVLSNWEMFMSK------RFD--KVRNRCRKGIPEGIRPRAWQYLCGSRQNIFKFKGQYH 424
EV + WE +S +FD K+ + +G+P R W++L F K Q+
Sbjct: 769 EVTTVWEKMLSTPGRSKIKFDMEKMHSAVGQGVPRHHRGEIWKFLAEQ----FHLKHQFP 824
Query: 425 ALIQQEGDIAVIDQIGK----------DVGRQYPQHEIXXXXXXXXXXXLFNVLKAFSVL 574
+ QQ D+ + + + D+GR +P H L+N+LKA+S+L
Sbjct: 825 SK-QQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLL 883
Query: 575 YPATGYCXXXXXXXXXXXXHMPEEEAFWTFCAICEHYLPGYFDSGLER--------VQLD 730
GYC HM EEEAF + FD GL + +Q+
Sbjct: 884 DQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFL-------MFDMGLRKQYRPDMIILQIQ 936
Query: 731 AKILYGLLKRHVPQAYKHLKLHEVEPVYFMIDWFMCIFTRNFP*ATVVRVLDMFF 895
L LL + Y HL+ HE+ P + WF+ +F FP V RV DM F
Sbjct: 937 MYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIF 991
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,033,477
Number of Sequences: 369166
Number of extensions: 2052570
Number of successful extensions: 5478
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5241
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5440
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9030416440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)