Planaria EST Database

DrC_00726

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00726
         (873 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P31937|D3HI_HUMAN  3-hydroxyisobutyrate dehydrogenase, mi...   221   2e-57
sp|Q99L13|3HIDH_MOUSE  3-hydroxyisobutyrate dehydrogenase, m...   220   5e-57
sp|P29266|3HIDH_RAT  3-hydroxyisobutyrate dehydrogenase, mit...   218   2e-56
sp|Q9XTI0|3HIDH_CAEEL  Probable 3-hydroxyisobutyrate dehydro...   185   2e-46
sp|Q9V8M5|3HIDH_DROME  Probable 3-hydroxyisobutyrate dehydro...   183   6e-46
sp|Q9SUC0|D3HI_ARATH  Probable 3-hydroxyisobutyrate dehydrog...   171   2e-42
sp|P28811|MMSB_PSEAE  3-hydroxyisobutyrate dehydrogenase (HI...   162   9e-40
sp|P63935|MMSB_MYCTU  Probable 3-hydroxyisobutyrate dehydrog...   142   9e-34
sp|O34948|YKWC_BACSU  Hypothetical oxidoreductase ykwC            135   1e-31
sp|P0ABQ2|GARR_ECOLI  2-hydroxy-3-oxopropionate reductase (T...   121   2e-27

>sp|P31937|D3HI_HUMAN 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor
           (HIBADH)
          Length = 336

 Score =  221 bits (563), Expect = 2e-57
 Identities = 119/229 (51%), Positives = 154/229 (67%), Gaps = 1/229 (0%)
 Frame = +2

Query: 188 RYLSDDCKVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSL 367
           R ++    VGF+GLGNMG  MA NL+  G+ ++ YD      K  ++        VV S 
Sbjct: 34  RSVASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFQDAGE----QVVSSP 89

Query: 368 KDFSNFPDIKAVVTMLPSSPQVVDVYR-TDELIKNVAKNTLFIDCTTGDPAVSQMIAKEA 544
            D +   D   ++TMLP+S   ++ Y   + ++K V K +L ID +T DPAVS+ +AKE 
Sbjct: 90  ADVAEKAD--RIITMLPTSINAIEAYSGANGILKKVKKGSLLIDSSTIDPAVSKELAKEV 147

Query: 545 ESLGNYYVDAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLA 724
           E +G  ++DAPVSGGV AAR G LTFMVGG +D F  A  LL  MG N+ +CG++GTG A
Sbjct: 148 EKMGAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAVGTGQA 207

Query: 725 AKICNNMLLAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSDT 871
           AKICNNMLLAISMIG +EAMNLG +LGL+P +LAKI+N SSGRCWSSDT
Sbjct: 208 AKICNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDT 256

>sp|Q99L13|3HIDH_MOUSE 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor
           (HIBADH)
          Length = 335

 Score =  220 bits (560), Expect = 5e-57
 Identities = 119/229 (51%), Positives = 153/229 (66%), Gaps = 1/229 (0%)
 Frame = +2

Query: 188 RYLSDDCKVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSL 367
           R ++    VGF+GLGNMG  MA NL+  G+ ++ YD      K  KE        V  S 
Sbjct: 33  RSMASKTPVGFIGLGNMGNPMAKNLMKHGYPLILYDVFPDVCKEFKEAGE----QVASSP 88

Query: 368 KDFSNFPDIKAVVTMLPSSPQVVDVYR-TDELIKNVAKNTLFIDCTTGDPAVSQMIAKEA 544
            + +   D   ++TMLPSS   V+VY   + ++K V K +L ID +T DP+VS+ +AKE 
Sbjct: 89  AEVAEKAD--RIITMLPSSMNAVEVYSGANGILKKVKKGSLLIDSSTIDPSVSKELAKEV 146

Query: 545 ESLGNYYVDAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLA 724
           E +G  ++DAPVSGGV AAR G LTFMVGG +D F  A  LL  MG N+ +CG++GTG +
Sbjct: 147 EKMGAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLECMGSNVVYCGAVGTGQS 206

Query: 725 AKICNNMLLAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSDT 871
           AKICNNMLLAISMIG +EAMNLG + GL+P +LAKI+N SSGRCWSSDT
Sbjct: 207 AKICNNMLLAISMIGTAEAMNLGIRSGLDPKLLAKILNMSSGRCWSSDT 255

>sp|P29266|3HIDH_RAT 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor
           (HIBADH)
          Length = 335

 Score =  218 bits (554), Expect = 2e-56
 Identities = 118/229 (51%), Positives = 153/229 (66%), Gaps = 1/229 (0%)
 Frame = +2

Query: 188 RYLSDDCKVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSL 367
           R ++    VGF+GLGNMG  MA NLI  G+ ++ YD      K  KE        V  S 
Sbjct: 33  RSMASKTPVGFIGLGNMGNPMAKNLIKHGYPLILYDVFPDVCKEFKEAGE----QVASSP 88

Query: 368 KDFSNFPDIKAVVTMLPSSPQVVDVYR-TDELIKNVAKNTLFIDCTTGDPAVSQMIAKEA 544
            D +   D   ++TMLPSS   ++VY   + ++K V K +L ID +T DP+VS+ +AKE 
Sbjct: 89  ADVAEKAD--RIITMLPSSMNSIEVYSGANGILKKVKKGSLLIDSSTIDPSVSKELAKEV 146

Query: 545 ESLGNYYVDAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLA 724
           E +G  ++DAPVSGGV AAR G LTFMVGG ++ F  A  LL  MG N+ +CG++G+G +
Sbjct: 147 EKMGAVFMDAPVSGGVGAARSGNLTFMVGGVENEFAAAQELLGCMGSNVLYCGAVGSGQS 206

Query: 725 AKICNNMLLAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSDT 871
           AKICNNMLLAISMIG +EAMNLG + GL+P +LAKI+N SSGRCWSSDT
Sbjct: 207 AKICNNMLLAISMIGTAEAMNLGIRSGLDPKLLAKILNMSSGRCWSSDT 255

>sp|Q9XTI0|3HIDH_CAEEL Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial (HIBADH)
          Length = 299

 Score =  185 bits (469), Expect = 2e-46
 Identities = 103/221 (46%), Positives = 139/221 (62%), Gaps = 2/221 (0%)
 Frame = +2

Query: 215 GFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPDI 394
           GF+GLGNMG +MA NLI  G  ++ YD +   ++  K  A G EV+   +    ++    
Sbjct: 5   GFIGLGNMGGHMARNLIKNGKKLIVYDVNKAVVQEFK--AEGCEVAAHPADIAAAS---- 58

Query: 395 KAVVTMLPSSPQVVDVYRTDE-LIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNYYVD 571
           K ++T+LPSSP V  VY+ +  + K +   TL +D +T D  VS  +A+ A  L   Y+D
Sbjct: 59  KEIITVLPSSPHVKAVYQGEAGIFKTIQPGTLCMDSSTIDQIVSLEVAQAAALLKAEYID 118

Query: 572 APVSGGVNAARDGVLTFMVGGDQDR-FNQASVLLRKMGKNIFHCGSIGTGLAAKICNNML 748
           AP+SGGV  A+   LTFMVG   D  F +A  +L  MGKNI + G++G G AAKICNNML
Sbjct: 119 APISGGVTGAQQATLTFMVGAGNDATFKRAEAVLSLMGKNIVNLGAVGNGTAAKICNNML 178

Query: 749 LAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSDT 871
           L I M+ V+E MNLG  +GL+   LA I+N+SSGRCWSSDT
Sbjct: 179 LGIQMVAVAETMNLGISMGLDAKALAGIVNTSSGRCWSSDT 219

>sp|Q9V8M5|3HIDH_DROME Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
           precursor (HIBADH)
          Length = 324

 Score =  183 bits (464), Expect = 6e-46
 Identities = 100/219 (45%), Positives = 134/219 (61%)
 Frame = +2

Query: 212 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 391
           +GFVGLGNMGA MA NLI  GH +  +D        L      K  +V     + +   D
Sbjct: 31  IGFVGLGNMGANMASNLIKAGHKLHVFDISKPACDGLA----AKGATVYAKTSELAKNSD 86

Query: 392 IKAVVTMLPSSPQVVDVYRTDELIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNYYVD 571
              V+TMLP++  +VD    +     V K+T+FID +T  P + + + K+  + G  ++D
Sbjct: 87  F--VITMLPNNA-IVDASYDEMTADGVNKDTIFIDSSTISPDLVKSLQKKISAKGARFID 143

Query: 572 APVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLAAKICNNMLL 751
           APVSGGV  A    LTFMVGG +  +N    +L  MGK I HCG  G G AAK+CNNM+L
Sbjct: 144 APVSGGVPGAEQATLTFMVGGTEAEYNAVKAVLECMGKKITHCGVYGMGQAAKLCNNMML 203

Query: 752 AISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSD 868
           AISMIGVSEAMNL  + GL+ ++ A+IINSS+GRCW+S+
Sbjct: 204 AISMIGVSEAMNLAVRQGLDANVFAEIINSSTGRCWASE 242

>sp|Q9SUC0|D3HI_ARATH Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
           precursor (HIBADH)
          Length = 347

 Score =  171 bits (433), Expect = 2e-42
 Identities = 99/232 (42%), Positives = 139/232 (59%), Gaps = 13/232 (5%)
 Frame = +2

Query: 212 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 391
           VGF+GLGNMG  M  NLI  G+ +  +D +   +K   E+      +  +  +D      
Sbjct: 40  VGFIGLGNMGFRMVNNLIRAGYKVTVHDINRDVMKMFTEMGVSSRETPYEVAQDS----- 94

Query: 392 IKAVVTMLPSSPQVVDVYR-TDELI--KNVAKNTLFIDCTTGDPAVSQMIAKEA------ 544
            + V+TMLPSS  V+DVY  T+ L+  +N  +  LFID +T DP  ++ I+         
Sbjct: 95  -EVVITMLPSSSHVMDVYTGTNGLLLGENDIRPALFIDSSTIDPQTTRKISLAVSNCNLK 153

Query: 545 ESLGNY----YVDAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIG 712
           E   N+     +DAPVSGGV AA  G LTFMVGG +D +  A  +L+ MG+   +CG  G
Sbjct: 154 EKRDNWEKPVMLDAPVSGGVLAAEAGTLTFMVGGPEDAYLAARPILQSMGRTSIYCGGSG 213

Query: 713 TGLAAKICNNMLLAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSD 868
            G AAKICNN+ +A+SM+G SEA+ LG+ LG+  S L +++N+SSGRCWSSD
Sbjct: 214 NGSAAKICNNLAMAVSMLGTSEALALGQSLGISASTLTEVLNTSSGRCWSSD 265

>sp|P28811|MMSB_PSEAE 3-hydroxyisobutyrate dehydrogenase (HIBADH)
          Length = 298

 Score =  162 bits (411), Expect = 9e-40
 Identities = 89/218 (40%), Positives = 128/218 (58%), Gaps = 1/218 (0%)
 Frame = +2

Query: 212 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 391
           + F+GLGNMG  MA NL+  GH +  +D     +  L E       S ++  +       
Sbjct: 4   IAFLGLGNMGGPMAANLLKAGHRVNVFDLQPKAVLGLVEQGAQGADSALQCCEG------ 57

Query: 392 IKAVVTMLPSSPQVVDVYRTDE-LIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNYYV 568
            + V++MLP+   V  +Y  D+ L+  VA   L IDC+T  P  ++ +A+ A + G   +
Sbjct: 58  AEVVISMLPAGQHVESLYLGDDGLLARVAGKPLLIDCSTIAPETARKVAEAAAAKGLTLL 117

Query: 569 DAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLAAKICNNML 748
           DAPVSGGV  AR G L+F+VGG  + F +A  +L  MG+NIFH G  G G  AKICNNML
Sbjct: 118 DAPVSGGVGGARAGTLSFIVGGPAEGFARARPVLENMGRNIFHAGDHGAGQVAKICNNML 177

Query: 749 LAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWS 862
           L I M G +EA+ LG K GL+P++L++++  SSG  W+
Sbjct: 178 LGILMAGTAEALALGVKNGLDPAVLSEVMKQSSGGNWA 215

>sp|P63935|MMSB_MYCTU Probable 3-hydroxyisobutyrate dehydrogenase (HIBADH)
 sp|P63936|MMSB_MYCBO Probable 3-hydroxyisobutyrate dehydrogenase (HIBADH)
          Length = 294

 Score =  142 bits (359), Expect = 9e-34
 Identities = 84/220 (38%), Positives = 118/220 (53%)
 Frame = +2

Query: 212 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 391
           + F+GLGNMGA M+ NL+  GH +  +D            A    V+V +S  +     D
Sbjct: 4   IAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASG----AAAHGVAVFRSAPEAVAEAD 59

Query: 392 IKAVVTMLPSSPQVVDVYRTDELIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNYYVD 571
           +  V+TMLP+   V   Y   +++      TLFID +T     ++ +   AES G   +D
Sbjct: 60  V--VITMLPTGEVVRRCYT--DVLAAARPATLFIDSSTISVTDAREVHALAESHGMLQLD 115

Query: 572 APVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLAAKICNNMLL 751
           APVSGGV  A    L FMVGGD+    +A  +L  M   I HCG+ G G AAK+CNNM+L
Sbjct: 116 APVSGGVKGAAAATLAFMVGGDESTLRRARPVLEPMAGKIIHCGAAGAGQAAKVCNNMVL 175

Query: 752 AISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSDT 871
           A+  I ++EA  L +KLGL    L  +I  ++G CW+  T
Sbjct: 176 AVQQIAIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHT 215

>sp|O34948|YKWC_BACSU Hypothetical oxidoreductase ykwC
          Length = 288

 Score =  135 bits (341), Expect = 1e-31
 Identities = 76/218 (34%), Positives = 125/218 (57%), Gaps = 1/218 (0%)
 Frame = +2

Query: 212 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 391
           +GF+GLG MG  MA +++N GH ++ Y +   + +S+ +    K      ++KD S   D
Sbjct: 5   IGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQ----KGAIWKDTVKDLSKEAD 60

Query: 392 IKAVVTMLPSSPQVVDVY-RTDELIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNYYV 568
           +  ++TM+     V +VY  ++ +I+N  +    ID TT  P++++ IA+ A+    + +
Sbjct: 61  V--IITMVGYPSDVEEVYFGSNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFAL 118

Query: 569 DAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLAAKICNNML 748
           DAPVSGG   A++G L  MVGG+++ F     +   MG+NI + G  G+G   K+CN + 
Sbjct: 119 DAPVSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIA 178

Query: 749 LAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWS 862
           +A  MIGV+EAM   +K GLEP  + K I + +   WS
Sbjct: 179 IAAGMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWS 216

>sp|P0ABQ2|GARR_ECOLI 2-hydroxy-3-oxopropionate reductase (Tartronate semialdehyde
           reductase) (TSAR)
 sp|P0ABQ3|GARR_ECOL6 2-hydroxy-3-oxopropionate reductase (Tartronate semialdehyde
           reductase) (TSAR)
          Length = 294

 Score =  121 bits (304), Expect = 2e-27
 Identities = 72/211 (34%), Positives = 122/211 (57%), Gaps = 2/211 (0%)
 Frame = +2

Query: 209 KVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEV-SVVKSLKDFSNF 385
           KVGF+GLG MG  M+ NL+  G+++V  D++   I  +  +A G E  S  K++ +    
Sbjct: 2   KVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADV--IAAGAETASTAKAIAE---- 55

Query: 386 PDIKAVVTMLPSSPQVVDVYRTDE-LIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNY 562
                ++TMLP+SP V +V   +  +I+     T+ ID ++  P  S+ I++  ++ G  
Sbjct: 56  -QCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGID 114

Query: 563 YVDAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLAAKICNN 742
            +DAPVSGG   A DG L+ MVGGD+  F++   L++ M  ++ H G IG G   K+ N 
Sbjct: 115 MLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQ 174

Query: 743 MLLAISMIGVSEAMNLGKKLGLEPSILAKII 835
           +++A+++  +SEA+ L  K G+ P ++ + I
Sbjct: 175 VIVALNIAAMSEALTLATKAGVNPDLVYQAI 205

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,702,650
Number of Sequences: 369166
Number of extensions: 1960074
Number of successful extensions: 5724
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5495
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5697
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8646143400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00726

  1. Dr_sW_010_H22
  2. Dr_sW_018_P06