Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_P06
(873 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P31937|D3HI_HUMAN 3-hydroxyisobutyrate dehydrogenase, mi... 221 2e-57
sp|Q99L13|3HIDH_MOUSE 3-hydroxyisobutyrate dehydrogenase, m... 220 5e-57
sp|P29266|3HIDH_RAT 3-hydroxyisobutyrate dehydrogenase, mit... 218 2e-56
sp|Q9XTI0|3HIDH_CAEEL Probable 3-hydroxyisobutyrate dehydro... 185 2e-46
sp|Q9V8M5|3HIDH_DROME Probable 3-hydroxyisobutyrate dehydro... 183 6e-46
sp|Q9SUC0|D3HI_ARATH Probable 3-hydroxyisobutyrate dehydrog... 171 2e-42
sp|P28811|MMSB_PSEAE 3-hydroxyisobutyrate dehydrogenase (HI... 162 9e-40
sp|P63935|MMSB_MYCTU Probable 3-hydroxyisobutyrate dehydrog... 142 9e-34
sp|O34948|YKWC_BACSU Hypothetical oxidoreductase ykwC 135 1e-31
sp|P0ABQ2|GARR_ECOLI 2-hydroxy-3-oxopropionate reductase (T... 121 2e-27
>sp|P31937|D3HI_HUMAN 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor
(HIBADH)
Length = 336
Score = 221 bits (563), Expect = 2e-57
Identities = 119/229 (51%), Positives = 154/229 (67%), Gaps = 1/229 (0%)
Frame = +2
Query: 188 RYLSDDCKVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSL 367
R ++ VGF+GLGNMG MA NL+ G+ ++ YD K ++ VV S
Sbjct: 34 RSVASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFQDAGE----QVVSSP 89
Query: 368 KDFSNFPDIKAVVTMLPSSPQVVDVYR-TDELIKNVAKNTLFIDCTTGDPAVSQMIAKEA 544
D + D ++TMLP+S ++ Y + ++K V K +L ID +T DPAVS+ +AKE
Sbjct: 90 ADVAEKAD--RIITMLPTSINAIEAYSGANGILKKVKKGSLLIDSSTIDPAVSKELAKEV 147
Query: 545 ESLGNYYVDAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLA 724
E +G ++DAPVSGGV AAR G LTFMVGG +D F A LL MG N+ +CG++GTG A
Sbjct: 148 EKMGAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAVGTGQA 207
Query: 725 AKICNNMLLAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSDT 871
AKICNNMLLAISMIG +EAMNLG +LGL+P +LAKI+N SSGRCWSSDT
Sbjct: 208 AKICNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDT 256
>sp|Q99L13|3HIDH_MOUSE 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor
(HIBADH)
Length = 335
Score = 220 bits (560), Expect = 5e-57
Identities = 119/229 (51%), Positives = 153/229 (66%), Gaps = 1/229 (0%)
Frame = +2
Query: 188 RYLSDDCKVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSL 367
R ++ VGF+GLGNMG MA NL+ G+ ++ YD K KE V S
Sbjct: 33 RSMASKTPVGFIGLGNMGNPMAKNLMKHGYPLILYDVFPDVCKEFKEAGE----QVASSP 88
Query: 368 KDFSNFPDIKAVVTMLPSSPQVVDVYR-TDELIKNVAKNTLFIDCTTGDPAVSQMIAKEA 544
+ + D ++TMLPSS V+VY + ++K V K +L ID +T DP+VS+ +AKE
Sbjct: 89 AEVAEKAD--RIITMLPSSMNAVEVYSGANGILKKVKKGSLLIDSSTIDPSVSKELAKEV 146
Query: 545 ESLGNYYVDAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLA 724
E +G ++DAPVSGGV AAR G LTFMVGG +D F A LL MG N+ +CG++GTG +
Sbjct: 147 EKMGAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLECMGSNVVYCGAVGTGQS 206
Query: 725 AKICNNMLLAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSDT 871
AKICNNMLLAISMIG +EAMNLG + GL+P +LAKI+N SSGRCWSSDT
Sbjct: 207 AKICNNMLLAISMIGTAEAMNLGIRSGLDPKLLAKILNMSSGRCWSSDT 255
>sp|P29266|3HIDH_RAT 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor
(HIBADH)
Length = 335
Score = 218 bits (554), Expect = 2e-56
Identities = 118/229 (51%), Positives = 153/229 (66%), Gaps = 1/229 (0%)
Frame = +2
Query: 188 RYLSDDCKVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSL 367
R ++ VGF+GLGNMG MA NLI G+ ++ YD K KE V S
Sbjct: 33 RSMASKTPVGFIGLGNMGNPMAKNLIKHGYPLILYDVFPDVCKEFKEAGE----QVASSP 88
Query: 368 KDFSNFPDIKAVVTMLPSSPQVVDVYR-TDELIKNVAKNTLFIDCTTGDPAVSQMIAKEA 544
D + D ++TMLPSS ++VY + ++K V K +L ID +T DP+VS+ +AKE
Sbjct: 89 ADVAEKAD--RIITMLPSSMNSIEVYSGANGILKKVKKGSLLIDSSTIDPSVSKELAKEV 146
Query: 545 ESLGNYYVDAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLA 724
E +G ++DAPVSGGV AAR G LTFMVGG ++ F A LL MG N+ +CG++G+G +
Sbjct: 147 EKMGAVFMDAPVSGGVGAARSGNLTFMVGGVENEFAAAQELLGCMGSNVLYCGAVGSGQS 206
Query: 725 AKICNNMLLAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSDT 871
AKICNNMLLAISMIG +EAMNLG + GL+P +LAKI+N SSGRCWSSDT
Sbjct: 207 AKICNNMLLAISMIGTAEAMNLGIRSGLDPKLLAKILNMSSGRCWSSDT 255
>sp|Q9XTI0|3HIDH_CAEEL Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial (HIBADH)
Length = 299
Score = 185 bits (469), Expect = 2e-46
Identities = 103/221 (46%), Positives = 139/221 (62%), Gaps = 2/221 (0%)
Frame = +2
Query: 215 GFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPDI 394
GF+GLGNMG +MA NLI G ++ YD + ++ K A G EV+ + ++
Sbjct: 5 GFIGLGNMGGHMARNLIKNGKKLIVYDVNKAVVQEFK--AEGCEVAAHPADIAAAS---- 58
Query: 395 KAVVTMLPSSPQVVDVYRTDE-LIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNYYVD 571
K ++T+LPSSP V VY+ + + K + TL +D +T D VS +A+ A L Y+D
Sbjct: 59 KEIITVLPSSPHVKAVYQGEAGIFKTIQPGTLCMDSSTIDQIVSLEVAQAAALLKAEYID 118
Query: 572 APVSGGVNAARDGVLTFMVGGDQDR-FNQASVLLRKMGKNIFHCGSIGTGLAAKICNNML 748
AP+SGGV A+ LTFMVG D F +A +L MGKNI + G++G G AAKICNNML
Sbjct: 119 APISGGVTGAQQATLTFMVGAGNDATFKRAEAVLSLMGKNIVNLGAVGNGTAAKICNNML 178
Query: 749 LAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSDT 871
L I M+ V+E MNLG +GL+ LA I+N+SSGRCWSSDT
Sbjct: 179 LGIQMVAVAETMNLGISMGLDAKALAGIVNTSSGRCWSSDT 219
>sp|Q9V8M5|3HIDH_DROME Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
precursor (HIBADH)
Length = 324
Score = 183 bits (464), Expect = 6e-46
Identities = 100/219 (45%), Positives = 134/219 (61%)
Frame = +2
Query: 212 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 391
+GFVGLGNMGA MA NLI GH + +D L K +V + + D
Sbjct: 31 IGFVGLGNMGANMASNLIKAGHKLHVFDISKPACDGLA----AKGATVYAKTSELAKNSD 86
Query: 392 IKAVVTMLPSSPQVVDVYRTDELIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNYYVD 571
V+TMLP++ +VD + V K+T+FID +T P + + + K+ + G ++D
Sbjct: 87 F--VITMLPNNA-IVDASYDEMTADGVNKDTIFIDSSTISPDLVKSLQKKISAKGARFID 143
Query: 572 APVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLAAKICNNMLL 751
APVSGGV A LTFMVGG + +N +L MGK I HCG G G AAK+CNNM+L
Sbjct: 144 APVSGGVPGAEQATLTFMVGGTEAEYNAVKAVLECMGKKITHCGVYGMGQAAKLCNNMML 203
Query: 752 AISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSD 868
AISMIGVSEAMNL + GL+ ++ A+IINSS+GRCW+S+
Sbjct: 204 AISMIGVSEAMNLAVRQGLDANVFAEIINSSTGRCWASE 242
>sp|Q9SUC0|D3HI_ARATH Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
precursor (HIBADH)
Length = 347
Score = 171 bits (433), Expect = 2e-42
Identities = 99/232 (42%), Positives = 139/232 (59%), Gaps = 13/232 (5%)
Frame = +2
Query: 212 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 391
VGF+GLGNMG M NLI G+ + +D + +K E+ + + +D
Sbjct: 40 VGFIGLGNMGFRMVNNLIRAGYKVTVHDINRDVMKMFTEMGVSSRETPYEVAQDS----- 94
Query: 392 IKAVVTMLPSSPQVVDVYR-TDELI--KNVAKNTLFIDCTTGDPAVSQMIAKEA------ 544
+ V+TMLPSS V+DVY T+ L+ +N + LFID +T DP ++ I+
Sbjct: 95 -EVVITMLPSSSHVMDVYTGTNGLLLGENDIRPALFIDSSTIDPQTTRKISLAVSNCNLK 153
Query: 545 ESLGNY----YVDAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIG 712
E N+ +DAPVSGGV AA G LTFMVGG +D + A +L+ MG+ +CG G
Sbjct: 154 EKRDNWEKPVMLDAPVSGGVLAAEAGTLTFMVGGPEDAYLAARPILQSMGRTSIYCGGSG 213
Query: 713 TGLAAKICNNMLLAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSD 868
G AAKICNN+ +A+SM+G SEA+ LG+ LG+ S L +++N+SSGRCWSSD
Sbjct: 214 NGSAAKICNNLAMAVSMLGTSEALALGQSLGISASTLTEVLNTSSGRCWSSD 265
>sp|P28811|MMSB_PSEAE 3-hydroxyisobutyrate dehydrogenase (HIBADH)
Length = 298
Score = 162 bits (411), Expect = 9e-40
Identities = 89/218 (40%), Positives = 128/218 (58%), Gaps = 1/218 (0%)
Frame = +2
Query: 212 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 391
+ F+GLGNMG MA NL+ GH + +D + L E S ++ +
Sbjct: 4 IAFLGLGNMGGPMAANLLKAGHRVNVFDLQPKAVLGLVEQGAQGADSALQCCEG------ 57
Query: 392 IKAVVTMLPSSPQVVDVYRTDE-LIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNYYV 568
+ V++MLP+ V +Y D+ L+ VA L IDC+T P ++ +A+ A + G +
Sbjct: 58 AEVVISMLPAGQHVESLYLGDDGLLARVAGKPLLIDCSTIAPETARKVAEAAAAKGLTLL 117
Query: 569 DAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLAAKICNNML 748
DAPVSGGV AR G L+F+VGG + F +A +L MG+NIFH G G G AKICNNML
Sbjct: 118 DAPVSGGVGGARAGTLSFIVGGPAEGFARARPVLENMGRNIFHAGDHGAGQVAKICNNML 177
Query: 749 LAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWS 862
L I M G +EA+ LG K GL+P++L++++ SSG W+
Sbjct: 178 LGILMAGTAEALALGVKNGLDPAVLSEVMKQSSGGNWA 215
>sp|P63935|MMSB_MYCTU Probable 3-hydroxyisobutyrate dehydrogenase (HIBADH)
sp|P63936|MMSB_MYCBO Probable 3-hydroxyisobutyrate dehydrogenase (HIBADH)
Length = 294
Score = 142 bits (359), Expect = 9e-34
Identities = 84/220 (38%), Positives = 118/220 (53%)
Frame = +2
Query: 212 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 391
+ F+GLGNMGA M+ NL+ GH + +D A V+V +S + D
Sbjct: 4 IAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASG----AAAHGVAVFRSAPEAVAEAD 59
Query: 392 IKAVVTMLPSSPQVVDVYRTDELIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNYYVD 571
+ V+TMLP+ V Y +++ TLFID +T ++ + AES G +D
Sbjct: 60 V--VITMLPTGEVVRRCYT--DVLAAARPATLFIDSSTISVTDAREVHALAESHGMLQLD 115
Query: 572 APVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLAAKICNNMLL 751
APVSGGV A L FMVGGD+ +A +L M I HCG+ G G AAK+CNNM+L
Sbjct: 116 APVSGGVKGAAAATLAFMVGGDESTLRRARPVLEPMAGKIIHCGAAGAGQAAKVCNNMVL 175
Query: 752 AISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWSSDT 871
A+ I ++EA L +KLGL L +I ++G CW+ T
Sbjct: 176 AVQQIAIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHT 215
>sp|O34948|YKWC_BACSU Hypothetical oxidoreductase ykwC
Length = 288
Score = 135 bits (341), Expect = 1e-31
Identities = 76/218 (34%), Positives = 125/218 (57%), Gaps = 1/218 (0%)
Frame = +2
Query: 212 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 391
+GF+GLG MG MA +++N GH ++ Y + + +S+ + K ++KD S D
Sbjct: 5 IGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQ----KGAIWKDTVKDLSKEAD 60
Query: 392 IKAVVTMLPSSPQVVDVY-RTDELIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNYYV 568
+ ++TM+ V +VY ++ +I+N + ID TT P++++ IA+ A+ + +
Sbjct: 61 V--IITMVGYPSDVEEVYFGSNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFAL 118
Query: 569 DAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLAAKICNNML 748
DAPVSGG A++G L MVGG+++ F + MG+NI + G G+G K+CN +
Sbjct: 119 DAPVSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIA 178
Query: 749 LAISMIGVSEAMNLGKKLGLEPSILAKIINSSSGRCWS 862
+A MIGV+EAM +K GLEP + K I + + WS
Sbjct: 179 IAAGMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWS 216
>sp|P0ABQ2|GARR_ECOLI 2-hydroxy-3-oxopropionate reductase (Tartronate semialdehyde
reductase) (TSAR)
sp|P0ABQ3|GARR_ECOL6 2-hydroxy-3-oxopropionate reductase (Tartronate semialdehyde
reductase) (TSAR)
Length = 294
Score = 121 bits (304), Expect = 2e-27
Identities = 72/211 (34%), Positives = 122/211 (57%), Gaps = 2/211 (0%)
Frame = +2
Query: 209 KVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEV-SVVKSLKDFSNF 385
KVGF+GLG MG M+ NL+ G+++V D++ I + +A G E S K++ +
Sbjct: 2 KVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADV--IAAGAETASTAKAIAE---- 55
Query: 386 PDIKAVVTMLPSSPQVVDVYRTDE-LIKNVAKNTLFIDCTTGDPAVSQMIAKEAESLGNY 562
++TMLP+SP V +V + +I+ T+ ID ++ P S+ I++ ++ G
Sbjct: 56 -QCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGID 114
Query: 563 YVDAPVSGGVNAARDGVLTFMVGGDQDRFNQASVLLRKMGKNIFHCGSIGTGLAAKICNN 742
+DAPVSGG A DG L+ MVGGD+ F++ L++ M ++ H G IG G K+ N
Sbjct: 115 MLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQ 174
Query: 743 MLLAISMIGVSEAMNLGKKLGLEPSILAKII 835
+++A+++ +SEA+ L K G+ P ++ + I
Sbjct: 175 VIVALNIAAMSEALTLATKAGVNPDLVYQAI 205
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,702,650
Number of Sequences: 369166
Number of extensions: 1960074
Number of successful extensions: 5724
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5495
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5697
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8646143400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)