Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_H22
(509 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P29266|3HIDH_RAT 3-hydroxyisobutyrate dehydrogenase, mit... 60 2e-09
sp|Q99L13|3HIDH_MOUSE 3-hydroxyisobutyrate dehydrogenase, m... 59 7e-09
sp|Q9SUC0|D3HI_ARATH Probable 3-hydroxyisobutyrate dehydrog... 58 1e-08
sp|P31937|D3HI_HUMAN 3-hydroxyisobutyrate dehydrogenase, mi... 57 2e-08
sp|Q9XTI0|3HIDH_CAEEL Probable 3-hydroxyisobutyrate dehydro... 54 2e-07
sp|Q9V8M5|3HIDH_DROME Probable 3-hydroxyisobutyrate dehydro... 52 1e-06
sp|P0ABQ2|GARR_ECOLI 2-hydroxy-3-oxopropionate reductase (T... 48 1e-05
sp|O34948|YKWC_BACSU Hypothetical oxidoreductase ykwC 45 1e-04
sp|P0A9V8|YIHU_ECOLI Hypothetical oxidoreductase yihU >gi|7... 43 4e-04
sp|P28811|MMSB_PSEAE 3-hydroxyisobutyrate dehydrogenase (HI... 42 6e-04
>sp|P29266|3HIDH_RAT 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor
(HIBADH)
Length = 335
Score = 60.5 bits (145), Expect = 2e-09
Identities = 43/114 (37%), Positives = 59/114 (51%), Gaps = 2/114 (1%)
Frame = +1
Query: 172 RYLSDDCKVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSL 351
R ++ VGF+GLGNMG MA NLI G+ ++ YD K KE V S
Sbjct: 33 RSMASKTPVGFIGLGNMGNPMAKNLIKHGYPLILYDVFPDVCKEFKEAGE----QVASSP 88
Query: 352 KDFSNFPDIKAVVTMLPSSPQVVDVYRLMN--**KCCQNTLFIDCTTGDPAVSQ 507
D + D ++TMLPSS ++VY N K + +L ID +T DP+VS+
Sbjct: 89 ADVAEKAD--RIITMLPSSMNSIEVYSGANGILKKVKKGSLLIDSSTIDPSVSK 140
>sp|Q99L13|3HIDH_MOUSE 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor
(HIBADH)
Length = 335
Score = 58.9 bits (141), Expect = 7e-09
Identities = 42/114 (36%), Positives = 59/114 (51%), Gaps = 2/114 (1%)
Frame = +1
Query: 172 RYLSDDCKVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSL 351
R ++ VGF+GLGNMG MA NL+ G+ ++ YD K KE V S
Sbjct: 33 RSMASKTPVGFIGLGNMGNPMAKNLMKHGYPLILYDVFPDVCKEFKEAGE----QVASSP 88
Query: 352 KDFSNFPDIKAVVTMLPSSPQVVDVYRLMN--**KCCQNTLFIDCTTGDPAVSQ 507
+ + D ++TMLPSS V+VY N K + +L ID +T DP+VS+
Sbjct: 89 AEVAEKAD--RIITMLPSSMNAVEVYSGANGILKKVKKGSLLIDSSTIDPSVSK 140
>sp|Q9SUC0|D3HI_ARATH Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
precursor (HIBADH)
Length = 347
Score = 57.8 bits (138), Expect = 1e-08
Identities = 39/108 (36%), Positives = 54/108 (50%), Gaps = 4/108 (3%)
Frame = +1
Query: 196 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 375
VGF+GLGNMG M NLI G+ + +D + +K E+ V + D
Sbjct: 40 VGFIGLGNMGFRMVNNLIRAGYKVTVHDINRDVMKMFTEM------GVSSRETPYEVAQD 93
Query: 376 IKAVVTMLPSSPQVVDVYRLMN**KCCQN----TLFIDCTTGDPAVSQ 507
+ V+TMLPSS V+DVY N +N LFID +T DP ++
Sbjct: 94 SEVVITMLPSSSHVMDVYTGTNGLLLGENDIRPALFIDSSTIDPQTTR 141
>sp|P31937|D3HI_HUMAN 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor
(HIBADH)
Length = 336
Score = 57.4 bits (137), Expect = 2e-08
Identities = 40/114 (35%), Positives = 59/114 (51%), Gaps = 2/114 (1%)
Frame = +1
Query: 172 RYLSDDCKVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSL 351
R ++ VGF+GLGNMG MA NL+ G+ ++ YD K ++ VV S
Sbjct: 34 RSVASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFQDAGE----QVVSSP 89
Query: 352 KDFSNFPDIKAVVTMLPSSPQVVDVYRLMN--**KCCQNTLFIDCTTGDPAVSQ 507
D + D ++TMLP+S ++ Y N K + +L ID +T DPAVS+
Sbjct: 90 ADVAEKAD--RIITMLPTSINAIEAYSGANGILKKVKKGSLLIDSSTIDPAVSK 141
>sp|Q9XTI0|3HIDH_CAEEL Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial (HIBADH)
Length = 299
Score = 53.9 bits (128), Expect = 2e-07
Identities = 32/78 (41%), Positives = 46/78 (58%)
Frame = +1
Query: 199 GFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPDI 378
GF+GLGNMG +MA NLI G ++ YD + ++ K A G EV+ + D +
Sbjct: 5 GFIGLGNMGGHMARNLIKNGKKLIVYDVNKAVVQEFK--AEGCEVAAHPA--DIA--AAS 58
Query: 379 KAVVTMLPSSPQVVDVYR 432
K ++T+LPSSP V VY+
Sbjct: 59 KEIITVLPSSPHVKAVYQ 76
>sp|Q9V8M5|3HIDH_DROME Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
precursor (HIBADH)
Length = 324
Score = 51.6 bits (122), Expect = 1e-06
Identities = 36/100 (36%), Positives = 49/100 (49%)
Frame = +1
Query: 196 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 375
+GFVGLGNMGA MA NLI GH + +D L K +V + + D
Sbjct: 31 IGFVGLGNMGANMASNLIKAGHKLHVFDISKPACDGL----AAKGATVYAKTSELAKNSD 86
Query: 376 IKAVVTMLPSSPQVVDVYRLMN**KCCQNTLFIDCTTGDP 495
V+TMLP++ V Y M ++T+FID +T P
Sbjct: 87 F--VITMLPNNAIVDASYDEMTADGVNKDTIFIDSSTISP 124
>sp|P0ABQ2|GARR_ECOLI 2-hydroxy-3-oxopropionate reductase (Tartronate semialdehyde
reductase) (TSAR)
sp|P0ABQ3|GARR_ECOL6 2-hydroxy-3-oxopropionate reductase (Tartronate semialdehyde
reductase) (TSAR)
Length = 294
Score = 48.1 bits (113), Expect = 1e-05
Identities = 29/79 (36%), Positives = 46/79 (58%), Gaps = 1/79 (1%)
Frame = +1
Query: 193 KVGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKE-VSVVKSLKDFSNF 369
KVGF+GLG MG M+ NL+ G+++V D++ I + +A G E S K++ +
Sbjct: 2 KVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADV--IAAGAETASTAKAIAE---- 55
Query: 370 PDIKAVVTMLPSSPQVVDV 426
++TMLP+SP V +V
Sbjct: 56 -QCDVIITMLPNSPHVKEV 73
>sp|O34948|YKWC_BACSU Hypothetical oxidoreductase ykwC
Length = 288
Score = 44.7 bits (104), Expect = 1e-04
Identities = 30/106 (28%), Positives = 54/106 (50%), Gaps = 2/106 (1%)
Frame = +1
Query: 196 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 375
+GF+GLG MG MA +++N GH ++ Y + + +S+ + K ++KD S D
Sbjct: 5 IGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQ----KGAIWKDTVKDLSKEAD 60
Query: 376 IKAVVTMLPSSPQVVDVYRLMN--**KCCQNTLFIDCTTGDPAVSQ 507
+ ++TM+ V +VY N + ID TT P++++
Sbjct: 61 V--IITMVGYPSDVEEVYFGSNGIIENAKEGAYLIDMTTSKPSLAK 104
>sp|P0A9V8|YIHU_ECOLI Hypothetical oxidoreductase yihU
sp|P0A9V9|YIHU_SHIFL Hypothetical oxidoreductase yihU
Length = 298
Score = 43.1 bits (100), Expect = 4e-04
Identities = 22/77 (28%), Positives = 42/77 (54%)
Frame = +1
Query: 196 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 375
+ F+GLG MG+ MA NL+ +GH + +D ++ ++ L + + ++ K D
Sbjct: 4 IAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAK------D 57
Query: 376 IKAVVTMLPSSPQVVDV 426
+ ++TMLP+ V +V
Sbjct: 58 AEFIITMLPNGDLVRNV 74
>sp|P28811|MMSB_PSEAE 3-hydroxyisobutyrate dehydrogenase (HIBADH)
Length = 298
Score = 42.4 bits (98), Expect = 6e-04
Identities = 30/108 (27%), Positives = 49/108 (45%), Gaps = 4/108 (3%)
Frame = +1
Query: 196 VGFVGLGNMGAYMAVNLINKGHNIVAYDKDSGRIKSLKELAPGKEVSVVKSLKDFSNFPD 375
+ F+GLGNMG MA NL+ GH + +D + L E S ++ +
Sbjct: 4 IAFLGLGNMGGPMAANLLKAGHRVNVFDLQPKAVLGLVEQGAQGADSALQCCE------G 57
Query: 376 IKAVVTMLPSSPQVVDVY----RLMN**KCCQNTLFIDCTTGDPAVSQ 507
+ V++MLP+ V +Y L+ + L IDC+T P ++
Sbjct: 58 AEVVISMLPAGQHVESLYLGDDGLLA--RVAGKPLLIDCSTIAPETAR 103
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,512,242
Number of Sequences: 369166
Number of extensions: 1074940
Number of successful extensions: 2984
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2902
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2977
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3255600150
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)