Planaria EST Database


DrC_00725

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00725
         (825 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q61792|LASP1_MOUSE  LIM and SH3 domain protein 1 (LASP-1)...   186   5e-47
sp|Q99MZ8|LASP1_RAT  LIM and SH3 domain protein 1 (LASP-1)        186   7e-47
sp|Q14847|LASP1_HUMAN  LIM and SH3 domain protein 1 (LASP-1)...   184   2e-46
sp|Q8I7C3|LAS1_DROME  LIM and SH3 domain protein Lasp             132   9e-31
sp|P34416|LASP1_CAEEL  LIM and SH3 domain protein F42H10.3        124   2e-28
sp|P20929|NEBU_HUMAN  Nebulin                                      84   5e-16
sp|O76041|NEBL_HUMAN  Nebulette (Actin-binding Z-disk protein)     77   8e-14
sp|Q9BX66|SRBS1_HUMAN  Sorbin and SH3 domain-containing prot...    60   9e-09
sp|P38479|ABP1_SACEX  Actin-binding protein                        59   2e-08
sp|P29675|SF3_HELAN  Pollen-specific protein SF3                   58   4e-08
>sp|Q61792|LASP1_MOUSE LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
          Length = 263

 Score =  186 bits (473), Expect = 5e-47
 Identities = 101/259 (38%), Positives = 130/259 (50%), Gaps = 52/259 (20%)
 Frame = +1

Query: 22  CSRCNKTVYPVEKLSCLDKYWHKGCFKCTH*ELTLNMKNYKGYNKMPYCSAHYPTQKFTT 201
           C+RC K VYP EK++CLDKYWHK CF C   ++TLNMKNYKGY K PYC+AHYP Q FT 
Sbjct: 5   CARCGKIVYPTEKVNCLDKYWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHYPKQSFTM 64

Query: 202 ITDTPENKRLAQLTQ------------------------------------NVSNINYHK 273
           + DTPEN RL Q ++                                     +SNI YH+
Sbjct: 65  VADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVADTPELQRIKKTQDQISNIKYHE 124

Query: 274 QFNEERNNFKSYETNNPPQRFENNDNNANDDGRQNEQAP----------------RITKI 405
           +F + R      E   P +R   + ++      Q +  P                ++T  
Sbjct: 125 EFEKSRMGPSGGEGVEPERREAQDSSSYRRPTEQQQPQPHHIPTSAPVYQQPQQQQMTSS 184

Query: 406 NARQVLPQSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTG 585
                 P +PV    S       +Y+ +Y+Y A D DEVSF +GD I+N + ID GWM G
Sbjct: 185 YGGYKEPAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYG 244

Query: 586 TVERTGEHGMLPFNYVEKI 642
           TVERTG+ GMLP NYVE I
Sbjct: 245 TVERTGDTGMLPANYVEAI 263
>sp|Q99MZ8|LASP1_RAT LIM and SH3 domain protein 1 (LASP-1)
          Length = 263

 Score =  186 bits (472), Expect = 7e-47
 Identities = 102/259 (39%), Positives = 132/259 (50%), Gaps = 52/259 (20%)
 Frame = +1

Query: 22  CSRCNKTVYPVEKLSCLDKYWHKGCFKCTH*ELTLNMKNYKGYNKMPYCSAHYPTQKFTT 201
           C+RC K VYP EK++CLDK+WHK CF C   ++TLNMKNYKGY K PYC+AHYP Q FT 
Sbjct: 5   CARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHYPKQSFTM 64

Query: 202 ITDTPENKRLAQLTQ------------------------------------NVSNINYHK 273
           + DTPEN RL Q ++                                     +SNI YH+
Sbjct: 65  VADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVADTPELQRIKKTQDQISNIKYHE 124

Query: 274 QFNEERNNFKSYETNNPPQRFENNDNNANDDGRQNEQAPRITKINA--------RQVLPQ 429
           +F + R      E   P +R   + ++      Q +  P     +A        +QV P 
Sbjct: 125 EFEKSRMGPSGGEGIEPERREAQDSSSYRRPTEQQQPQPHHIPTSAPVYQQPQQQQVTPS 184

Query: 430 --------SPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTG 585
                   +PV    S       +Y+ +Y+Y A D DEVSF +GD I+N + ID GWM G
Sbjct: 185 YGGYKEPAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYG 244

Query: 586 TVERTGEHGMLPFNYVEKI 642
           TVERTG+ GMLP NYVE I
Sbjct: 245 TVERTGDTGMLPANYVEAI 263
>sp|Q14847|LASP1_HUMAN LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
          Length = 261

 Score =  184 bits (468), Expect = 2e-46
 Identities = 104/258 (40%), Positives = 133/258 (51%), Gaps = 51/258 (19%)
 Frame = +1

Query: 22  CSRCNKTVYPVEKLSCLDKYWHKGCFKCTH*ELTLNMKNYKGYNKMPYCSAHYPTQKFTT 201
           C+RC K VYP EK++CLDK+WHK CF C   ++TLNMKNYKGY K PYC+AHYP Q FT 
Sbjct: 5   CARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHYPKQSFTM 64

Query: 202 ITDTPENKRLAQLTQ------------------------------------NVSNINYHK 273
           + DTPEN RL Q ++                                     +SNI YH+
Sbjct: 65  VADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVADTPELQRIKKTQDQISNIKYHE 124

Query: 274 QFNEERNNFKSYETNNPPQRFENNDNNANDDGRQNEQAPRI-TKINARQVLPQSPV-QRV 447
           +F + R      E    P+R ++ D ++     + +Q   I T     Q   Q PV Q  
Sbjct: 125 EFEKSRMGPSGGE-GMEPERRDSQDGSSYRRPLEQQQPHHIPTSAPVYQQPQQQPVAQSY 183

Query: 448 PSQVEPEK-------------VKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGT 588
               EP                +Y+ +Y+Y A D DEVSF +GD I+N + ID GWM GT
Sbjct: 184 GGYKEPAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYGT 243

Query: 589 VERTGEHGMLPFNYVEKI 642
           VERTG+ GMLP NYVE I
Sbjct: 244 VERTGDTGMLPANYVEAI 261
>sp|Q8I7C3|LAS1_DROME LIM and SH3 domain protein Lasp
          Length = 657

 Score =  132 bits (333), Expect = 9e-31
 Identities = 58/116 (50%), Positives = 74/116 (63%)
 Frame = +1

Query: 16  RQCSRCNKTVYPVEKLSCLDKYWHKGCFKCTH*ELTLNMKNYKGYNKMPYCSAHYPTQKF 195
           + C+RC K VYP+E+L CLDK WHK CFKCT   +TLNMK YKGYNKMPYC AH P  K 
Sbjct: 3   KTCARCQKVVYPIEELKCLDKTWHKTCFKCTECGMTLNMKTYKGYNKMPYCEAHIPKAKA 62

Query: 196 TTITDTPENKRLAQLTQNVSNINYHKQFNEERNNFKSYETNNPPQRFENNDNNAND 363
           T I DTPE KR+A+ T+  SN+ YH  F + +  F     +    R + N  + ++
Sbjct: 63  TAIADTPELKRIAENTKIQSNVKYHADFEKAKGKFTQVADDPETLRIKQNTKHISN 118

 Score = 79.7 bits (195), Expect = 9e-15
 Identities = 43/90 (47%), Positives = 55/90 (61%)
 Frame = +1

Query: 370 RQNEQAPRITKINARQVLPQSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIII 549
           +Q E  P+  ++  RQ    S +Q   S+       Y+ +Y+Y A D DEVSF EGD+I 
Sbjct: 567 QQQEHPPQQQQM--RQQPSYSSLQEKQSRQSTAMRVYRAIYDYEAQDVDEVSFREGDVIF 624

Query: 550 NGEVIDQGWMTGTVERTGEHGMLPFNYVEK 639
             E ID GWMTG VERTG+ GMLP NYVE+
Sbjct: 625 EVESIDSGWMTGRVERTGKTGMLPANYVEQ 654
>sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3
          Length = 335

 Score =  124 bits (312), Expect = 2e-28
 Identities = 62/156 (39%), Positives = 95/156 (60%), Gaps = 5/156 (3%)
 Frame = +1

Query: 13  TRQCSR--CNKTVYPVEKLSCLDKYWHKGCFKCTH*ELTLNMKNYKGYNKMPYCSAHYPT 186
           +++C+R  C KTVYPVE+L CLDK WHK CFKCT   +TLNMKNYKGY+K PYC  HYP 
Sbjct: 2   SKKCAREDCGKTVYPVEELKCLDKVWHKQCFKCTVCGMTLNMKNYKGYDKRPYCDPHYPK 61

Query: 187 QKFTTITDTPENKRLAQLTQNVSNINYHKQFNEERNNFKSYETNNPPQRFENN---DNNA 357
              + + DTPE +R+A+ T+N SNI YH ++ + +        +   +R + N    +N 
Sbjct: 62  TVASVMADTPEMRRIAENTKNQSNIKYHAEYEKMKGTKIEIADDPEMERLKKNTQVQSNV 121

Query: 358 NDDGRQNEQAPRITKINARQVLPQSPVQRVPSQVEP 465
           +  G  +++A R  ++  ++ +  +P    P+ + P
Sbjct: 122 SYHGVLDQKA-RQEEVRPKEEISPNP---TPTPISP 153

 Score = 77.0 bits (188), Expect = 6e-14
 Identities = 40/87 (45%), Positives = 51/87 (58%), Gaps = 3/87 (3%)
 Frame = +1

Query: 385 APRITKINARQVLPQSPVQRVPSQVEPEK---VKYKCLYEYMATDNDEVSFSEGDIIING 555
           AP  +  +     P S +    S + P        K +Y+Y A D DE+SF EGDII+N 
Sbjct: 237 APPPSSNSISSTSPHSTLSSPQSTISPTGKAGFAVKAIYDYAAADKDEISFLEGDIIVNC 296

Query: 556 EVIDQGWMTGTVERTGEHGMLPFNYVE 636
           E ID GWMTGTV+RT + GMLP NYV+
Sbjct: 297 EKIDDGWMTGTVQRTLQWGMLPANYVQ 323
>sp|P20929|NEBU_HUMAN Nebulin
          Length = 6669

 Score = 84.0 bits (206), Expect = 5e-16
 Identities = 39/72 (54%), Positives = 49/72 (68%)
 Frame = +1

Query: 427  QSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGE 606
            Q+ V  +PS        ++ +Y+YMA D DEVSF +GD IIN + ID+GWM GTV+RTG 
Sbjct: 6598 QTTVSSIPSHPSTAGKIFRAMYDYMAADADEVSFKDGDAIINVQAIDEGWMYGTVQRTGR 6657

Query: 607  HGMLPFNYVEKI 642
             GMLP NYVE I
Sbjct: 6658 TGMLPANYVEAI 6669

 Score = 36.2 bits (82), Expect = 0.11
 Identities = 15/58 (25%), Positives = 31/58 (53%)
 Frame = +1

Query: 190  KFTTITDTPENKRLAQLTQNVSNINYHKQFNEERNNFKSYETNNPPQRFENNDNNAND 363
            K   + DTPE +R+ +  +++S + YH+ F + +  F    T+   +R + N  + +D
Sbjct: 6413 KAAYVLDTPEMRRVRETQRHISTVKYHEDFEKHKGCFTPVVTDPITERVKKNMQDFSD 6470

 Score = 33.9 bits (76), Expect = 0.56
 Identities = 23/81 (28%), Positives = 39/81 (48%), Gaps = 5/81 (6%)
 Frame = +1

Query: 133 KNYKGYNKMPYCSAH----YPTQ-KFTTITDTPENKRLAQLTQNVSNINYHKQFNEERNN 297
           + Y    K+  C  H    +P + KFT +TD+P   +    T+ +S++NY  +   ER  
Sbjct: 724 QEYDAIKKLDQCKDHTYKVHPDKTKFTAVTDSPVLLQAQLNTKQLSDLNYKAKHEGER-- 781

Query: 298 FKSYETNNPPQRFENNDNNAN 360
           FK +   + PQ  ++  N  N
Sbjct: 782 FKCHIPADAPQFIQHRVNAYN 802

 Score = 33.9 bits (76), Expect = 0.56
 Identities = 13/34 (38%), Positives = 24/34 (70%)
 Frame = +1

Query: 190  KFTTITDTPENKRLAQLTQNVSNINYHKQFNEER 291
            KFT+I DTPE+ R  ++ + +S+I Y  ++N+ +
Sbjct: 5451 KFTSIVDTPEHLRTTKVNKQISDILYKLEYNKAK 5484

 Score = 32.7 bits (73), Expect = 1.2
 Identities = 18/72 (25%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
 Frame = +1

Query: 118  LTLNMKNYKGYNKMP-YCSAHYPTQKFTTITDTPENKRLAQLTQNVSNINYHKQFNEERN 294
            L   + N    NK   +   H     +TT+ +T +   + ++T++VS+ NY K+F +E+ 
Sbjct: 5639 LAKTVSNLVSENKYKDHVKKHLAQGSYTTLPETRDTVHVKEVTKHVSDTNYKKKFVKEKG 5698

Query: 295  NFKSYETNNPPQ 330
                     PP+
Sbjct: 5699 KSNYSIMLEPPE 5710

 Score = 30.0 bits (66), Expect = 8.1
 Identities = 20/81 (24%), Positives = 39/81 (48%), Gaps = 5/81 (6%)
 Frame = +1

Query: 133 KNYKGYNKMPYCSAH----YPTQ-KFTTITDTPENKRLAQLTQNVSNINYHKQFNEERNN 297
           + Y+   K+  C  H    +P + KFT +TD+P   +    ++ +S++NY  +   E+  
Sbjct: 477 QEYEAIKKLDQCKDHTYKVHPDKTKFTQVTDSPVLLQAQVNSKQLSDLNYKAKHESEK-- 534

Query: 298 FKSYETNNPPQRFENNDNNAN 360
           FK +   + P   ++  N  N
Sbjct: 535 FKCHIPPDTPAFIQHKVNAYN 555
>sp|O76041|NEBL_HUMAN Nebulette (Actin-binding Z-disk protein)
          Length = 1014

 Score = 76.6 bits (187), Expect = 8e-14
 Identities = 34/61 (55%), Positives = 42/61 (68%)
 Frame = +1

Query: 454  QVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNYV 633
            Q  P    Y+ +Y+Y A D DEVSF +GD I+N + ID GWM GTV+RTG  GMLP NY+
Sbjct: 951  QHSPNLRTYRAMYDYSAQDEDEVSFRDGDYIVNVQPIDDGWMYGTVQRTGRTGMLPANYI 1010

Query: 634  E 636
            E
Sbjct: 1011 E 1011

 Score = 30.8 bits (68), Expect = 4.7
 Identities = 15/45 (33%), Positives = 26/45 (57%)
 Frame = +1

Query: 196 TTITDTPENKRLAQLTQNVSNINYHKQFNEERNNFKSYETNNPPQ 330
           T I+D PE KR+ +  +N+SN+ Y +Q      N+K+   +  P+
Sbjct: 634 TAISDPPELKRVKENQKNISNLQYKEQ------NYKATPVSMTPE 672

 Score = 30.4 bits (67), Expect = 6.2
 Identities = 25/98 (25%), Positives = 49/98 (50%), Gaps = 2/98 (2%)
 Frame = +1

Query: 196 TTITDTPENKRLAQLTQNVSNINYHKQFNEERNNFKSYETNNPPQRFENNDNNANDDGRQ 375
           T + D+PE +R+ +  QN+S++ Y ++         +   ++PP+     +N  N    Q
Sbjct: 603 TAVKDSPEIERVKKNQQNISSVKYKEEIKH------ATAISDPPELKRVKENQKNISNLQ 656

Query: 376 -NEQAPRITKINARQVLPQSPVQRV-PSQVEPEKVKYK 483
             EQ  + T ++   + P+  ++RV  +Q +   VKYK
Sbjct: 657 YKEQNYKATPVS---MTPE--IERVRRNQEQLSAVKYK 689

 Score = 30.0 bits (66), Expect = 8.1
 Identities = 12/28 (42%), Positives = 19/28 (67%)
 Frame = +1

Query: 193 FTTITDTPENKRLAQLTQNVSNINYHKQ 276
           ++TI DTPE +R+    QN+S + Y K+
Sbjct: 571 YSTIADTPEIQRIKTTQQNISAVFYKKE 598
>sp|Q9BX66|SRBS1_HUMAN Sorbin and SH3 domain-containing protein 1 (Ponsin) (c-Cbl-associated
            protein) (CAP) (SH3 domain protein 5) (SH3P12)
          Length = 1292

 Score = 59.7 bits (143), Expect = 9e-09
 Identities = 34/106 (32%), Positives = 50/106 (47%), Gaps = 3/106 (2%)
 Frame = +1

Query: 334  FENNDNNANDDGRQNEQAPRITKINARQVLPQSPVQRVP---SQVEPEKVKYKCLYEYMA 504
            F  ++    +  R  EQA R          PQ+  +RV    SQ   +   Y+ LY Y+ 
Sbjct: 1185 FPGSEAEQTERHRGGEQAGRKAARRGGSQQPQAQQRRVTPDRSQTSQDLFSYQALYSYIP 1244

Query: 505  TDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNYVEKI 642
             ++DE+   +GDI+   E  D GW  GT  RT + G  P NYV+ +
Sbjct: 1245 QNDDELELRDGDIVDVMEKCDDGWFVGTSRRTKQFGTFPGNYVKPL 1290

 Score = 33.9 bits (76), Expect = 0.56
 Identities = 19/62 (30%), Positives = 31/62 (50%)
 Frame = +1

Query: 451 SQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNY 630
           S++ P + K+    ++ A    E+   +GDI+   + IDQ W  G  E  G  G+ P  Y
Sbjct: 793 SEMRPARAKF----DFKAQTLKELPLQKGDIVYIYKQIDQNWYEG--EHHGRVGIFPRTY 846

Query: 631 VE 636
           +E
Sbjct: 847 IE 848

 Score = 33.1 bits (74), Expect = 0.95
 Identities = 14/42 (33%), Positives = 22/42 (52%)
 Frame = +1

Query: 517  EVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNYVEKI 642
            E+SF +G+ I     +D+ W  G +  T   G+ P  YV+ I
Sbjct: 885  EMSFRKGERITLLRQVDENWYEGRIPGTSRQGIFPITYVDVI 926
>sp|P38479|ABP1_SACEX Actin-binding protein
          Length = 617

 Score = 58.9 bits (141), Expect = 2e-08
 Identities = 33/89 (37%), Positives = 49/89 (55%), Gaps = 2/89 (2%)
 Frame = +1

Query: 373 QNEQAPRITKINARQVLPQSPVQRVPSQVEP--EKVKYKCLYEYMATDNDEVSFSEGDII 546
           + E+AP    + +R  +P  P QR     EP  E       Y+Y A +++E++F+E D I
Sbjct: 526 EEEEAPA-PSLPSRGSVPPPPPQRAVEPEEPAAEAPWATAEYDYEAGEDNELTFAENDKI 584

Query: 547 INGEVIDQGWMTGTVERTGEHGMLPFNYV 633
           IN E +D  W  G +E TG+ G+ P NYV
Sbjct: 585 INIEFVDDDWWLGELETTGQKGLFPSNYV 613
>sp|P29675|SF3_HELAN Pollen-specific protein SF3
          Length = 219

 Score = 57.8 bits (138), Expect = 4e-08
 Identities = 22/56 (39%), Positives = 35/56 (62%)
 Frame = +1

Query: 13  TRQCSRCNKTVYPVEKLSCLDKYWHKGCFKCTH*ELTLNMKNYKGYNKMPYCSAHY 180
           T++C+ C KTVY V+KL    + +HK CF+C H   TL + N+  ++ + YC  H+
Sbjct: 8   TQKCTVCEKTVYLVDKLVANQRVYHKACFRCHHCNSTLKLSNFNSFDGVVYCRHHF 63

 Score = 51.6 bits (122), Expect = 3e-06
 Identities = 22/58 (37%), Positives = 33/58 (56%), Gaps = 1/58 (1%)
 Frame = +1

Query: 10  GTR-QCSRCNKTVYPVEKLSCLDKYWHKGCFKCTH*ELTLNMKNYKGYNKMPYCSAHY 180
           GTR +C+ C K VYP+E++      +H+ CFKC H   T++  NY  +    YC  H+
Sbjct: 105 GTRDKCNACAKIVYPIERVKVDGTAYHRACFKCCHGGCTISPSNYIAHEGRLYCKHHH 162
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,757,272
Number of Sequences: 369166
Number of extensions: 1740924
Number of successful extensions: 6904
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5316
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6184
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7956112725
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00725

  1. Dr_sW_018_O11
  2. Dr_sW_004_B18