Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_O11
(824 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q61792|LASP1_MOUSE LIM and SH3 domain protein 1 (LASP-1)... 191 2e-48
sp|Q99MZ8|LASP1_RAT LIM and SH3 domain protein 1 (LASP-1) 191 2e-48
sp|Q14847|LASP1_HUMAN LIM and SH3 domain protein 1 (LASP-1)... 189 6e-48
sp|Q8I7C3|LAS1_DROME LIM and SH3 domain protein Lasp 137 3e-32
sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3 129 7e-30
sp|P20929|NEBU_HUMAN Nebulin 84 5e-16
sp|O76041|NEBL_HUMAN Nebulette (Actin-binding Z-disk protein) 77 8e-14
sp|P29675|SF3_HELAN Pollen-specific protein SF3 63 1e-09
sp|Q9BX66|SRBS1_HUMAN Sorbin and SH3 domain-containing prot... 60 9e-09
sp|P38479|ABP1_SACEX Actin-binding protein 59 2e-08
>sp|Q61792|LASP1_MOUSE LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
Length = 263
Score = 191 bits (486), Expect = 2e-48
Identities = 102/259 (39%), Positives = 131/259 (50%), Gaps = 52/259 (20%)
Frame = +1
Query: 22 CSRCNKTVYPVEKLSCLDKYWHKGCFKCTHCELTLNMKNYKGYNKMPYCSAHYPTQKFTT 201
C+RC K VYP EK++CLDKYWHK CF C C++TLNMKNYKGY K PYC+AHYP Q FT
Sbjct: 5 CARCGKIVYPTEKVNCLDKYWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHYPKQSFTM 64
Query: 202 ITDTPENKRLAQLTQ------------------------------------NVSNINYHK 273
+ DTPEN RL Q ++ +SNI YH+
Sbjct: 65 VADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVADTPELQRIKKTQDQISNIKYHE 124
Query: 274 QFNEERNNFKSYETNNPPQRFENNDNNANDDGRQNEQAP----------------RITKI 405
+F + R E P +R + ++ Q + P ++T
Sbjct: 125 EFEKSRMGPSGGEGVEPERREAQDSSSYRRPTEQQQPQPHHIPTSAPVYQQPQQQQMTSS 184
Query: 406 NARQVLPQSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTG 585
P +PV S +Y+ +Y+Y A D DEVSF +GD I+N + ID GWM G
Sbjct: 185 YGGYKEPAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYG 244
Query: 586 TVERTGEHGMLPFNYVEKI 642
TVERTG+ GMLP NYVE I
Sbjct: 245 TVERTGDTGMLPANYVEAI 263
>sp|Q99MZ8|LASP1_RAT LIM and SH3 domain protein 1 (LASP-1)
Length = 263
Score = 191 bits (485), Expect = 2e-48
Identities = 103/259 (39%), Positives = 133/259 (51%), Gaps = 52/259 (20%)
Frame = +1
Query: 22 CSRCNKTVYPVEKLSCLDKYWHKGCFKCTHCELTLNMKNYKGYNKMPYCSAHYPTQKFTT 201
C+RC K VYP EK++CLDK+WHK CF C C++TLNMKNYKGY K PYC+AHYP Q FT
Sbjct: 5 CARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHYPKQSFTM 64
Query: 202 ITDTPENKRLAQLTQ------------------------------------NVSNINYHK 273
+ DTPEN RL Q ++ +SNI YH+
Sbjct: 65 VADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVADTPELQRIKKTQDQISNIKYHE 124
Query: 274 QFNEERNNFKSYETNNPPQRFENNDNNANDDGRQNEQAPRITKINA--------RQVLPQ 429
+F + R E P +R + ++ Q + P +A +QV P
Sbjct: 125 EFEKSRMGPSGGEGIEPERREAQDSSSYRRPTEQQQPQPHHIPTSAPVYQQPQQQQVTPS 184
Query: 430 --------SPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTG 585
+PV S +Y+ +Y+Y A D DEVSF +GD I+N + ID GWM G
Sbjct: 185 YGGYKEPAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYG 244
Query: 586 TVERTGEHGMLPFNYVEKI 642
TVERTG+ GMLP NYVE I
Sbjct: 245 TVERTGDTGMLPANYVEAI 263
>sp|Q14847|LASP1_HUMAN LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
Length = 261
Score = 189 bits (481), Expect = 6e-48
Identities = 105/258 (40%), Positives = 134/258 (51%), Gaps = 51/258 (19%)
Frame = +1
Query: 22 CSRCNKTVYPVEKLSCLDKYWHKGCFKCTHCELTLNMKNYKGYNKMPYCSAHYPTQKFTT 201
C+RC K VYP EK++CLDK+WHK CF C C++TLNMKNYKGY K PYC+AHYP Q FT
Sbjct: 5 CARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHYPKQSFTM 64
Query: 202 ITDTPENKRLAQLTQ------------------------------------NVSNINYHK 273
+ DTPEN RL Q ++ +SNI YH+
Sbjct: 65 VADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVADTPELQRIKKTQDQISNIKYHE 124
Query: 274 QFNEERNNFKSYETNNPPQRFENNDNNANDDGRQNEQAPRI-TKINARQVLPQSPV-QRV 447
+F + R E P+R ++ D ++ + +Q I T Q Q PV Q
Sbjct: 125 EFEKSRMGPSGGE-GMEPERRDSQDGSSYRRPLEQQQPHHIPTSAPVYQQPQQQPVAQSY 183
Query: 448 PSQVEPEK-------------VKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGT 588
EP +Y+ +Y+Y A D DEVSF +GD I+N + ID GWM GT
Sbjct: 184 GGYKEPAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYGT 243
Query: 589 VERTGEHGMLPFNYVEKI 642
VERTG+ GMLP NYVE I
Sbjct: 244 VERTGDTGMLPANYVEAI 261
>sp|Q8I7C3|LAS1_DROME LIM and SH3 domain protein Lasp
Length = 657
Score = 137 bits (346), Expect = 3e-32
Identities = 59/116 (50%), Positives = 75/116 (64%)
Frame = +1
Query: 16 RQCSRCNKTVYPVEKLSCLDKYWHKGCFKCTHCELTLNMKNYKGYNKMPYCSAHYPTQKF 195
+ C+RC K VYP+E+L CLDK WHK CFKCT C +TLNMK YKGYNKMPYC AH P K
Sbjct: 3 KTCARCQKVVYPIEELKCLDKTWHKTCFKCTECGMTLNMKTYKGYNKMPYCEAHIPKAKA 62
Query: 196 TTITDTPENKRLAQLTQNVSNINYHKQFNEERNNFKSYETNNPPQRFENNDNNAND 363
T I DTPE KR+A+ T+ SN+ YH F + + F + R + N + ++
Sbjct: 63 TAIADTPELKRIAENTKIQSNVKYHADFEKAKGKFTQVADDPETLRIKQNTKHISN 118
Score = 79.7 bits (195), Expect = 9e-15
Identities = 43/90 (47%), Positives = 55/90 (61%)
Frame = +1
Query: 370 RQNEQAPRITKINARQVLPQSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIII 549
+Q E P+ ++ RQ S +Q S+ Y+ +Y+Y A D DEVSF EGD+I
Sbjct: 567 QQQEHPPQQQQM--RQQPSYSSLQEKQSRQSTAMRVYRAIYDYEAQDVDEVSFREGDVIF 624
Query: 550 NGEVIDQGWMTGTVERTGEHGMLPFNYVEK 639
E ID GWMTG VERTG+ GMLP NYVE+
Sbjct: 625 EVESIDSGWMTGRVERTGKTGMLPANYVEQ 654
>sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3
Length = 335
Score = 129 bits (325), Expect = 7e-30
Identities = 63/156 (40%), Positives = 96/156 (61%), Gaps = 5/156 (3%)
Frame = +1
Query: 13 TRQCSR--CNKTVYPVEKLSCLDKYWHKGCFKCTHCELTLNMKNYKGYNKMPYCSAHYPT 186
+++C+R C KTVYPVE+L CLDK WHK CFKCT C +TLNMKNYKGY+K PYC HYP
Sbjct: 2 SKKCAREDCGKTVYPVEELKCLDKVWHKQCFKCTVCGMTLNMKNYKGYDKRPYCDPHYPK 61
Query: 187 QKFTTITDTPENKRLAQLTQNVSNINYHKQFNEERNNFKSYETNNPPQRFENN---DNNA 357
+ + DTPE +R+A+ T+N SNI YH ++ + + + +R + N +N
Sbjct: 62 TVASVMADTPEMRRIAENTKNQSNIKYHAEYEKMKGTKIEIADDPEMERLKKNTQVQSNV 121
Query: 358 NDDGRQNEQAPRITKINARQVLPQSPVQRVPSQVEP 465
+ G +++A R ++ ++ + +P P+ + P
Sbjct: 122 SYHGVLDQKA-RQEEVRPKEEISPNP---TPTPISP 153
Score = 77.0 bits (188), Expect = 6e-14
Identities = 40/87 (45%), Positives = 51/87 (58%), Gaps = 3/87 (3%)
Frame = +1
Query: 385 APRITKINARQVLPQSPVQRVPSQVEPEK---VKYKCLYEYMATDNDEVSFSEGDIIING 555
AP + + P S + S + P K +Y+Y A D DE+SF EGDII+N
Sbjct: 237 APPPSSNSISSTSPHSTLSSPQSTISPTGKAGFAVKAIYDYAAADKDEISFLEGDIIVNC 296
Query: 556 EVIDQGWMTGTVERTGEHGMLPFNYVE 636
E ID GWMTGTV+RT + GMLP NYV+
Sbjct: 297 EKIDDGWMTGTVQRTLQWGMLPANYVQ 323
>sp|P20929|NEBU_HUMAN Nebulin
Length = 6669
Score = 84.0 bits (206), Expect = 5e-16
Identities = 39/72 (54%), Positives = 49/72 (68%)
Frame = +1
Query: 427 QSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGE 606
Q+ V +PS ++ +Y+YMA D DEVSF +GD IIN + ID+GWM GTV+RTG
Sbjct: 6598 QTTVSSIPSHPSTAGKIFRAMYDYMAADADEVSFKDGDAIINVQAIDEGWMYGTVQRTGR 6657
Query: 607 HGMLPFNYVEKI 642
GMLP NYVE I
Sbjct: 6658 TGMLPANYVEAI 6669
Score = 36.2 bits (82), Expect = 0.11
Identities = 15/58 (25%), Positives = 31/58 (53%)
Frame = +1
Query: 190 KFTTITDTPENKRLAQLTQNVSNINYHKQFNEERNNFKSYETNNPPQRFENNDNNAND 363
K + DTPE +R+ + +++S + YH+ F + + F T+ +R + N + +D
Sbjct: 6413 KAAYVLDTPEMRRVRETQRHISTVKYHEDFEKHKGCFTPVVTDPITERVKKNMQDFSD 6470
Score = 33.9 bits (76), Expect = 0.56
Identities = 23/81 (28%), Positives = 39/81 (48%), Gaps = 5/81 (6%)
Frame = +1
Query: 133 KNYKGYNKMPYCSAH----YPTQ-KFTTITDTPENKRLAQLTQNVSNINYHKQFNEERNN 297
+ Y K+ C H +P + KFT +TD+P + T+ +S++NY + ER
Sbjct: 724 QEYDAIKKLDQCKDHTYKVHPDKTKFTAVTDSPVLLQAQLNTKQLSDLNYKAKHEGER-- 781
Query: 298 FKSYETNNPPQRFENNDNNAN 360
FK + + PQ ++ N N
Sbjct: 782 FKCHIPADAPQFIQHRVNAYN 802
Score = 33.9 bits (76), Expect = 0.56
Identities = 13/34 (38%), Positives = 24/34 (70%)
Frame = +1
Query: 190 KFTTITDTPENKRLAQLTQNVSNINYHKQFNEER 291
KFT+I DTPE+ R ++ + +S+I Y ++N+ +
Sbjct: 5451 KFTSIVDTPEHLRTTKVNKQISDILYKLEYNKAK 5484
Score = 32.7 bits (73), Expect = 1.2
Identities = 18/72 (25%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
Frame = +1
Query: 118 LTLNMKNYKGYNKMP-YCSAHYPTQKFTTITDTPENKRLAQLTQNVSNINYHKQFNEERN 294
L + N NK + H +TT+ +T + + ++T++VS+ NY K+F +E+
Sbjct: 5639 LAKTVSNLVSENKYKDHVKKHLAQGSYTTLPETRDTVHVKEVTKHVSDTNYKKKFVKEKG 5698
Query: 295 NFKSYETNNPPQ 330
PP+
Sbjct: 5699 KSNYSIMLEPPE 5710
Score = 30.0 bits (66), Expect = 8.1
Identities = 20/81 (24%), Positives = 39/81 (48%), Gaps = 5/81 (6%)
Frame = +1
Query: 133 KNYKGYNKMPYCSAH----YPTQ-KFTTITDTPENKRLAQLTQNVSNINYHKQFNEERNN 297
+ Y+ K+ C H +P + KFT +TD+P + ++ +S++NY + E+
Sbjct: 477 QEYEAIKKLDQCKDHTYKVHPDKTKFTQVTDSPVLLQAQVNSKQLSDLNYKAKHESEK-- 534
Query: 298 FKSYETNNPPQRFENNDNNAN 360
FK + + P ++ N N
Sbjct: 535 FKCHIPPDTPAFIQHKVNAYN 555
>sp|O76041|NEBL_HUMAN Nebulette (Actin-binding Z-disk protein)
Length = 1014
Score = 76.6 bits (187), Expect = 8e-14
Identities = 34/61 (55%), Positives = 42/61 (68%)
Frame = +1
Query: 454 QVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNYV 633
Q P Y+ +Y+Y A D DEVSF +GD I+N + ID GWM GTV+RTG GMLP NY+
Sbjct: 951 QHSPNLRTYRAMYDYSAQDEDEVSFRDGDYIVNVQPIDDGWMYGTVQRTGRTGMLPANYI 1010
Query: 634 E 636
E
Sbjct: 1011 E 1011
Score = 30.8 bits (68), Expect = 4.7
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +1
Query: 196 TTITDTPENKRLAQLTQNVSNINYHKQFNEERNNFKSYETNNPPQ 330
T I+D PE KR+ + +N+SN+ Y +Q N+K+ + P+
Sbjct: 634 TAISDPPELKRVKENQKNISNLQYKEQ------NYKATPVSMTPE 672
Score = 30.4 bits (67), Expect = 6.2
Identities = 25/98 (25%), Positives = 49/98 (50%), Gaps = 2/98 (2%)
Frame = +1
Query: 196 TTITDTPENKRLAQLTQNVSNINYHKQFNEERNNFKSYETNNPPQRFENNDNNANDDGRQ 375
T + D+PE +R+ + QN+S++ Y ++ + ++PP+ +N N Q
Sbjct: 603 TAVKDSPEIERVKKNQQNISSVKYKEEIKH------ATAISDPPELKRVKENQKNISNLQ 656
Query: 376 -NEQAPRITKINARQVLPQSPVQRV-PSQVEPEKVKYK 483
EQ + T ++ + P+ ++RV +Q + VKYK
Sbjct: 657 YKEQNYKATPVS---MTPE--IERVRRNQEQLSAVKYK 689
Score = 30.0 bits (66), Expect = 8.1
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +1
Query: 193 FTTITDTPENKRLAQLTQNVSNINYHKQ 276
++TI DTPE +R+ QN+S + Y K+
Sbjct: 571 YSTIADTPEIQRIKTTQQNISAVFYKKE 598
>sp|P29675|SF3_HELAN Pollen-specific protein SF3
Length = 219
Score = 62.8 bits (151), Expect = 1e-09
Identities = 23/56 (41%), Positives = 36/56 (64%)
Frame = +1
Query: 13 TRQCSRCNKTVYPVEKLSCLDKYWHKGCFKCTHCELTLNMKNYKGYNKMPYCSAHY 180
T++C+ C KTVY V+KL + +HK CF+C HC TL + N+ ++ + YC H+
Sbjct: 8 TQKCTVCEKTVYLVDKLVANQRVYHKACFRCHHCNSTLKLSNFNSFDGVVYCRHHF 63
Score = 52.0 bits (123), Expect = 2e-06
Identities = 22/58 (37%), Positives = 33/58 (56%), Gaps = 1/58 (1%)
Frame = +1
Query: 10 GTR-QCSRCNKTVYPVEKLSCLDKYWHKGCFKCTHCELTLNMKNYKGYNKMPYCSAHY 180
GTR +C+ C K VYP+E++ +H+ CFKC H T++ NY + YC H+
Sbjct: 105 GTRDKCNACAKIVYPIERVKVDGTAYHRACFKCCHGGCTISPSNYIAHEGRLYCKHHH 162
>sp|Q9BX66|SRBS1_HUMAN Sorbin and SH3 domain-containing protein 1 (Ponsin) (c-Cbl-associated
protein) (CAP) (SH3 domain protein 5) (SH3P12)
Length = 1292
Score = 59.7 bits (143), Expect = 9e-09
Identities = 34/106 (32%), Positives = 50/106 (47%), Gaps = 3/106 (2%)
Frame = +1
Query: 334 FENNDNNANDDGRQNEQAPRITKINARQVLPQSPVQRVP---SQVEPEKVKYKCLYEYMA 504
F ++ + R EQA R PQ+ +RV SQ + Y+ LY Y+
Sbjct: 1185 FPGSEAEQTERHRGGEQAGRKAARRGGSQQPQAQQRRVTPDRSQTSQDLFSYQALYSYIP 1244
Query: 505 TDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNYVEKI 642
++DE+ +GDI+ E D GW GT RT + G P NYV+ +
Sbjct: 1245 QNDDELELRDGDIVDVMEKCDDGWFVGTSRRTKQFGTFPGNYVKPL 1290
Score = 33.9 bits (76), Expect = 0.56
Identities = 19/62 (30%), Positives = 31/62 (50%)
Frame = +1
Query: 451 SQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNY 630
S++ P + K+ ++ A E+ +GDI+ + IDQ W G E G G+ P Y
Sbjct: 793 SEMRPARAKF----DFKAQTLKELPLQKGDIVYIYKQIDQNWYEG--EHHGRVGIFPRTY 846
Query: 631 VE 636
+E
Sbjct: 847 IE 848
Score = 33.1 bits (74), Expect = 0.95
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +1
Query: 517 EVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNYVEKI 642
E+SF +G+ I +D+ W G + T G+ P YV+ I
Sbjct: 885 EMSFRKGERITLLRQVDENWYEGRIPGTSRQGIFPITYVDVI 926
>sp|P38479|ABP1_SACEX Actin-binding protein
Length = 617
Score = 58.9 bits (141), Expect = 2e-08
Identities = 33/89 (37%), Positives = 49/89 (55%), Gaps = 2/89 (2%)
Frame = +1
Query: 373 QNEQAPRITKINARQVLPQSPVQRVPSQVEP--EKVKYKCLYEYMATDNDEVSFSEGDII 546
+ E+AP + +R +P P QR EP E Y+Y A +++E++F+E D I
Sbjct: 526 EEEEAPA-PSLPSRGSVPPPPPQRAVEPEEPAAEAPWATAEYDYEAGEDNELTFAENDKI 584
Query: 547 INGEVIDQGWMTGTVERTGEHGMLPFNYV 633
IN E +D W G +E TG+ G+ P NYV
Sbjct: 585 INIEFVDDDWWLGELETTGQKGLFPSNYV 613
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,753,598
Number of Sequences: 369166
Number of extensions: 1752005
Number of successful extensions: 7176
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5530
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6451
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7956112725
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)