Planaria EST Database


DrC_00724

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00724
         (663 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q6PFS7|APG3L_BRARE  Autophagy protein 3-like (APG3-like)       192   5e-49
sp|Q9NT62|APG3L_HUMAN  Autophagy protein 3-like (APG3-like) ...   188   1e-47
sp|Q9CPX6|APG3L_MOUSE  Autophagy protein 3-like (APG3-like)       188   1e-47
sp|Q6AZ50|APG3L_RAT  Autophagy protein 3-like (APG3-like) (P...   187   2e-47
sp|Q5I0S6|APG3L_XENTR  Autophagy protein 3-like (APG3-like)       187   2e-47
sp|Q6GQE7|APG3L_XENLA  Autophagy protein 3-like (APG3-like)       186   6e-47
sp|O43035|ATG3_SCHPO  Autophagy-related protein 3 (Autophagy...   108   1e-23
sp|Q6CL19|ATG3_KLULA  Autophagy-related protein 3 (Autophagy...   102   9e-22
sp|Q5K9X6|ATG3_CRYNE  Autophagy-related protein 3 (Autophagy...   101   2e-21
sp|Q755K1|ATG3_ASHGO  Autophagy-related protein 3 (Autophagy...    98   2e-20
>sp|Q6PFS7|APG3L_BRARE Autophagy protein 3-like (APG3-like)
          Length = 317

 Score =  192 bits (489), Expect = 5e-49
 Identities = 101/152 (66%), Positives = 113/152 (74%)
 Frame = +1

Query: 121 LEDDDLIAPANQSAMTSKEIIKVADSCQSDIVQTRTYDLQITYDKLYQTCRLWLCGYDED 300
           LE DD     ++ A  SK   K     +  I+QTRTYDL ITYDK YQT RLWL GYDED
Sbjct: 169 LETDDATLDTSKMADLSKT--KAEAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDED 226

Query: 301 RSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGA 480
           R  LT +QM+ED SQDHVKKTVT+E HP+LP    M SVHPCR AEVMKK+IE VAEGG 
Sbjct: 227 RQPLTVDQMYEDISQDHVKKTVTIENHPNLPPPA-MCSVHPCRHAEVMKKIIETVAEGGG 285

Query: 481 VLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 576
            LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct: 286 ELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 317
>sp|Q9NT62|APG3L_HUMAN Autophagy protein 3-like (APG3-like) (hApg3) (PC3-96 protein)
          Length = 314

 Score =  188 bits (477), Expect = 1e-47
 Identities = 95/139 (68%), Positives = 107/139 (76%), Gaps = 8/139 (5%)
 Frame = +1

Query: 184 KVADSCQSD--------IVQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDF 339
           K+ ++C++         I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED 
Sbjct: 177 KIVEACKAKTDAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDI 236

Query: 340 SQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLK 519
           SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLK
Sbjct: 237 SQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLK 295

Query: 520 FVQAVIPTIEYDYTRNFNM 576
           FVQAVIPTIEYDYTR+F M
Sbjct: 296 FVQAVIPTIEYDYTRHFTM 314
>sp|Q9CPX6|APG3L_MOUSE Autophagy protein 3-like (APG3-like)
          Length = 314

 Score =  188 bits (477), Expect = 1e-47
 Identities = 95/139 (68%), Positives = 107/139 (76%), Gaps = 8/139 (5%)
 Frame = +1

Query: 184 KVADSCQSD--------IVQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDF 339
           K+ ++C++         I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED 
Sbjct: 177 KIVEACKAKADAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDI 236

Query: 340 SQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLK 519
           SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLK
Sbjct: 237 SQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLK 295

Query: 520 FVQAVIPTIEYDYTRNFNM 576
           FVQAVIPTIEYDYTR+F M
Sbjct: 296 FVQAVIPTIEYDYTRHFTM 314
>sp|Q6AZ50|APG3L_RAT Autophagy protein 3-like (APG3-like) (Preconditioning-inducible
           gene 1 protein)
          Length = 314

 Score =  187 bits (476), Expect = 2e-47
 Identities = 97/147 (65%), Positives = 113/147 (76%), Gaps = 5/147 (3%)
 Frame = +1

Query: 151 NQSAMTSKEIIKV----ADSCQSD-IVQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLT 315
           +++ + ++ I++     AD+   D I+QTRTYDL ITYDK YQT RLWL GYDE R  LT
Sbjct: 169 DEATLDTRRIVEACKAKADAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLT 228

Query: 316 EEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVH 495
            E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH
Sbjct: 229 VEHMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVH 287

Query: 496 QYIVIFLKFVQAVIPTIEYDYTRNFNM 576
            Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct: 288 MYLLIFLKFVQAVIPTIEYDYTRHFTM 314
>sp|Q5I0S6|APG3L_XENTR Autophagy protein 3-like (APG3-like)
          Length = 312

 Score =  187 bits (475), Expect = 2e-47
 Identities = 99/148 (66%), Positives = 108/148 (72%), Gaps = 6/148 (4%)
 Frame = +1

Query: 151 NQSAMTSKEIIKVADSCQSD------IVQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLL 312
           N  A      IK A   ++D      I+QTRTYDL ITYDK YQT RLWL GYDE R  L
Sbjct: 166 NDDATVDTSKIKEACKPKADLGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRRPL 225

Query: 313 TEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGV 492
           T E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGV
Sbjct: 226 TVENMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGV 284

Query: 493 HQYIVIFLKFVQAVIPTIEYDYTRNFNM 576
           H Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct: 285 HMYLLIFLKFVQAVIPTIEYDYTRHFTM 312
>sp|Q6GQE7|APG3L_XENLA Autophagy protein 3-like (APG3-like)
          Length = 313

 Score =  186 bits (471), Expect = 6e-47
 Identities = 101/157 (64%), Positives = 114/157 (72%), Gaps = 6/157 (3%)
 Frame = +1

Query: 124 EDDDLIAPANQSAMTSKEIIKVADSCQSD------IVQTRTYDLQITYDKLYQTCRLWLC 285
           E+  L+   + +  TSK  IK A   ++D      I+QTRTYDL ITYDK YQT RLWL 
Sbjct: 160 EESGLLENDDATVDTSK--IKEACKPKADLGGEDAILQTRTYDLYITYDKYYQTPRLWLF 217

Query: 286 GYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAV 465
           GYDE R  L  E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE V
Sbjct: 218 GYDEQRRPLAVENMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETV 276

Query: 466 AEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 576
           AEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct: 277 AEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 313
>sp|O43035|ATG3_SCHPO Autophagy-related protein 3 (Autophagy-related E2-like conjugation
           enzyme atg3)
          Length = 275

 Score =  108 bits (270), Expect = 1e-23
 Identities = 56/113 (49%), Positives = 72/113 (63%)
 Frame = +1

Query: 223 RTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGV 402
           R YDL I YDK Y+T RL+L G++    LLT + ++ED S +H  KTVTME  PH     
Sbjct: 160 RYYDLYIVYDKYYRTPRLFLRGWNAGGQLLTMKDIYEDVSGEHAGKTVTMEPFPHYHSHN 219

Query: 403 LMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYT 561
            MASVHPC+ A V+ KLI+   E    + V QY+V+FLKFV  ++P  E DYT
Sbjct: 220 TMASVHPCKHASVLLKLIKQHRERNDPIRVDQYMVLFLKFVSTMLPYFEIDYT 272
>sp|Q6CL19|ATG3_KLULA Autophagy-related protein 3 (Autophagy-related E2-like conjugation
           enzyme ATG3)
          Length = 302

 Score =  102 bits (254), Expect = 9e-22
 Identities = 55/136 (40%), Positives = 75/136 (55%), Gaps = 23/136 (16%)
 Frame = +1

Query: 223 RTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGV 402
           R YDL ITY   Y+  +++LCGYD D + L+ +QMFED + D+  KT T+E  P L G  
Sbjct: 163 RFYDLYITYSTSYRVPKMYLCGYDNDGTPLSPDQMFEDIAADYRSKTATIEPLPFLKGNN 222

Query: 403 LMASVHPCRQAEVMKKLIEAVAEGGAV-----------------------LGVHQYIVIF 513
           +  S+HPC+ A VMK L+E V    +                        L V QY+V+F
Sbjct: 223 ISVSIHPCKHANVMKVLMEKVRSSRSRARKVDPQTTDEDWEDLQSDVDDGLRVDQYLVVF 282

Query: 514 LKFVQAVIPTIEYDYT 561
           LKF+ +V P IE+DYT
Sbjct: 283 LKFITSVTPGIEHDYT 298
>sp|Q5K9X6|ATG3_CRYNE Autophagy-related protein 3 (Autophagy-related E2-like conjugation
           enzyme ATG3)
          Length = 385

 Score =  101 bits (252), Expect = 2e-21
 Identities = 69/205 (33%), Positives = 93/205 (45%), Gaps = 58/205 (28%)
 Frame = +1

Query: 121 LEDDDLIAPANQSAMTSKEIIKVADSCQSDIVQTRTYDLQITYDKLYQTCRLWLCGYDED 300
           LEDD  +   + S        +V  +   +++Q RTYD  I+YDK YQT R WL GYDE 
Sbjct: 184 LEDDAAVRIVHPSEA------EVNSTAGKNLLQVRTYDCIISYDKHYQTPRFWLFGYDEH 237

Query: 301 RSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAV----- 465
           ++ LT  Q+F+D   DH  KT+TME  PH   G  +ASVHPC+ A VMKK I+ +     
Sbjct: 238 KNPLTPAQVFQDVPADHAFKTMTMESFPH--SGAQLASVHPCKHASVMKKFIDRMEAAQG 295

Query: 466 ----------------------------------------AEGGAVLGVHQ--------- 498
                                                     GG+V  V +         
Sbjct: 296 PAPTAEPETISSTSGTSGSAGGKEEKEKKKKWGLGSMVRKVTGGSVPKVEKDKDEVVTGV 355

Query: 499 ----YIVIFLKFVQAVIPTIEYDYT 561
               Y+VIFLKF+ +++PTIE D T
Sbjct: 356 PVDFYLVIFLKFIASIVPTIEVDST 380
>sp|Q755K1|ATG3_ASHGO Autophagy-related protein 3 (Autophagy-related E2-like conjugation
           enzyme ATG3)
          Length = 282

 Score = 97.8 bits (242), Expect = 2e-20
 Identities = 57/151 (37%), Positives = 81/151 (53%), Gaps = 29/151 (19%)
 Frame = +1

Query: 196 SCQSDIVQ-----TRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKK 360
           S + DI+Q     TR YDL ITY   Y+  +++L G++ D + LT +QMF+D + D+  K
Sbjct: 128 SGEQDIIQLRPSHTRFYDLYITYSTSYRVPKMYLVGFNNDGTPLTPQQMFQDIAPDYRTK 187

Query: 361 TVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVA------------------------ 468
           T T+E  P     V+  S+HPCR A VM+ L+E V                         
Sbjct: 188 TATIEKLPFFKSAVVAVSIHPCRHANVMRVLMEKVRAAKQKPVEEEQPDGpreDWEDLQD 247

Query: 469 EGGAVLGVHQYIVIFLKFVQAVIPTIEYDYT 561
           E  + L V QY+V+FLKF+ +V P IE+DYT
Sbjct: 248 EVDSSLRVVQYLVVFLKFITSVTPGIEHDYT 278
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,207,353
Number of Sequences: 369166
Number of extensions: 1112001
Number of successful extensions: 2630
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2547
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2617
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5560129980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00724

  1. Dr_sW_018_O03
  2. Dr_sW_008_L14