Planaria EST Database

DrC_00722

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00722
         (574 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P17764|THIL_RAT  Acetyl-CoA acetyltransferase, mitochondr...   226   3e-59
sp|Q8QZT1|THIL_MOUSE  Acetyl-CoA acetyltransferase, mitochon...   222   6e-58
sp|Q8HXY6|THIL_MACFA  Acetyl-CoA acetyltransferase, mitochon...   213   4e-55
sp|P24752|THIL_HUMAN  Acetyl-CoA acetyltransferase, mitochon...   212   5e-55
sp|Q6L8K7|THIL_YARLI  Acetyl-CoA acetyltransferase (Peroxiso...   167   2e-41
sp|P41338|THIL_YEAST  Acetyl-CoA acetyltransferase (Acetoace...   162   6e-40
sp|P10551|THIL_SACBA  Acetyl-CoA acetyltransferase (Acetoace...   158   1e-38
sp|Q12598|THIA_CANTR  Acetyl-CoA acetyltransferase IA (Perox...   151   1e-36
sp|Q9FIK7|THIC2_ARATH  Probable acetyl-CoA acetyltransferase...   151   1e-36
sp|Q04677|THIB_CANTR  Acetyl-CoA acetyltransferase IB (Perox...   151   1e-36

>sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondrial precursor
           (Acetoacetyl-CoA thiolase)
          Length = 424

 Score =  226 bits (576), Expect = 3e-59
 Identities = 112/174 (64%), Positives = 142/174 (81%), Gaps = 1/174 (0%)
 Frame = +1

Query: 1   DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
           DEEYKRV+F K   LK  F K NGTVTAAN+STLNDGAA+V+L++ EA  + K+ P+A+I
Sbjct: 250 DEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAAVVLMTAEAAQRLKVKPLARI 309

Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
             FADAA+ PIDFP+APA ++ K+LK      E+IAMWE+NEAFSVVVLANIK+ + +DP
Sbjct: 310 AAFADAAVDPIDFPLAPAYAVPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKMLE-IDP 368

Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 519
            KVN+HGGAVS+GHPIGMSG R+V+HLA+ LK  E G+ASICNGGGGAS+++I+
Sbjct: 369 QKVNVHGGAVSLGHPIGMSGARIVVHLAHALKQGEFGLASICNGGGGASAVLIE 422

>sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochondrial precursor
           (Acetoacetyl-CoA thiolase)
          Length = 424

 Score =  222 bits (565), Expect = 6e-58
 Identities = 109/174 (62%), Positives = 141/174 (81%), Gaps = 1/174 (0%)
 Frame = +1

Query: 1   DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
           DEEYKRV+F K   LK  F K NGT+TAAN+STLNDGAA+++L++ EA  +  + P+A+I
Sbjct: 250 DEEYKRVDFSKVPKLKTVFQKENGTITAANASTLNDGAAALVLMTAEAAQRLNVKPLARI 309

Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
             FADAA+ PIDFP+APA ++ K+LK      E+IAMWE+NEAFSVVVLANIK+ + +DP
Sbjct: 310 AAFADAAVDPIDFPLAPAYAVPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKMLE-IDP 368

Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 519
            KVNIHGGAVS+GHPIGMSG R+V+H+A+ LK  E G+ASICNGGGGAS+++I+
Sbjct: 369 QKVNIHGGAVSLGHPIGMSGARIVVHMAHALKPGEFGLASICNGGGGASALLIE 422

>sp|Q8HXY6|THIL_MACFA Acetyl-CoA acetyltransferase, mitochondrial precursor
           (Acetoacetyl-CoA thiolase)
          Length = 427

 Score =  213 bits (541), Expect = 4e-55
 Identities = 106/174 (60%), Positives = 138/174 (79%), Gaps = 1/174 (0%)
 Frame = +1

Query: 1   DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
           DEEYKRV+F K   LK  F K NGT+TAAN+STLNDGAA+++L++  A  +  + P+A+I
Sbjct: 253 DEEYKRVDFSKVPKLKTVFQKENGTITAANASTLNDGAAALVLMTAGAAKRLNVTPLARI 312

Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
           + FADAA++PIDFPIAP  ++  +LK      E+IAMWE+NEAFS+VVLANIK+ + +DP
Sbjct: 313 VAFADAAVEPIDFPIAPVHAVSMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLE-IDP 371

Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 519
            KVNI+GGAVS+GHPIGMSG R+V HL + LK  E G+ASICNGGGGAS+++IQ
Sbjct: 372 QKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQ 425

>sp|P24752|THIL_HUMAN Acetyl-CoA acetyltransferase, mitochondrial precursor
           (Acetoacetyl-CoA thiolase) (T2)
          Length = 427

 Score =  212 bits (540), Expect = 5e-55
 Identities = 107/174 (61%), Positives = 138/174 (79%), Gaps = 1/174 (0%)
 Frame = +1

Query: 1   DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
           DEEYKRV+F K   LK  F K NGTVTAAN+STLNDGAA+++L++ +A  +  + P+A+I
Sbjct: 253 DEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARI 312

Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
           + FADAA++PIDFPIAP  +   +LK      E+IAMWE+NEAFS+VVLANIK+ + +DP
Sbjct: 313 VAFADAAVEPIDFPIAPVYAASMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLE-IDP 371

Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 519
            KVNI+GGAVS+GHPIGMSG R+V HL + LK  E G+ASICNGGGGAS+++IQ
Sbjct: 372 QKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQ 425

>sp|Q6L8K7|THIL_YARLI Acetyl-CoA acetyltransferase (Peroxisomal acetoacetyl-CoA thiolase)
           (Thiolase)
          Length = 397

 Score =  167 bits (423), Expect = 2e-41
 Identities = 88/173 (50%), Positives = 118/173 (68%)
 Frame = +1

Query: 1   DEEYKRVNFEKFKSLKPAFKANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKII 180
           DEE K  N EK K LKP F   G+VTA N+S +NDGA++V++ S     +     +AKI+
Sbjct: 220 DEEPKGANAEKLKGLKPVFDKQGSVTAGNASPINDGASAVVVASGTKAKELGTPVLAKIV 279

Query: 181 GFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDPS 360
            +ADAA  PIDF IAP+ +I   LK      ++IA+WEINEAFS V LAN+ +  G+D S
Sbjct: 280 SYADAATAPIDFTIAPSLAIPAALKKAGLTKDDIALWEINEAFSGVALANL-MRLGIDKS 338

Query: 361 KVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 519
           KVN+ GGAV++GHPIG SG R+ + L   LK  E GVA+ICNGGG +++I+I+
Sbjct: 339 KVNVKGGAVALGHPIGASGNRIFVTLVNALKEGEYGVAAICNGGGASTAIVIK 391

>sp|P41338|THIL_YEAST Acetyl-CoA acetyltransferase (Acetoacetyl-CoA thiolase)
          Length = 398

 Score =  162 bits (410), Expect = 6e-40
 Identities = 92/177 (51%), Positives = 127/177 (71%), Gaps = 4/177 (2%)
 Frame = +1

Query: 1   DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
           DEE  R++ EK +S +  F K NGTVTAAN+S +NDGAA+V+LVS++ L +  L P+A I
Sbjct: 221 DEEPARLHVEKLRSARTVFQKENGTVTAANASPINDGAAAVILVSEKVLKEKNLKPLAII 280

Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICN-KNAEEIAMWEINEAFSVVVLANIKITKGMD 354
            G+ +AA QP DF  AP+ ++ K LK    ++   +  +E NEAFSVV L N KI K +D
Sbjct: 281 KGWGEAAHQPADFTWAPSLAVPKALKHAGIEDINSVDYFEFNEAFSVVGLVNTKILK-LD 339

Query: 355 PSKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKV--NELGVASICNGGGGASSIMIQ 519
           PSKVN++GGAV++GHP+G SG R+V+ L   L+    ++GVA+ICNGGGGASSI+I+
Sbjct: 340 PSKVNVYGGAVALGHPLGCSGARVVVTLLSILQQEGGKIGVAAICNGGGGASSIVIE 396

>sp|P10551|THIL_SACBA Acetyl-CoA acetyltransferase (Acetoacetyl-CoA thiolase)
          Length = 398

 Score =  158 bits (399), Expect = 1e-38
 Identities = 88/177 (49%), Positives = 125/177 (70%), Gaps = 4/177 (2%)
 Frame = +1

Query: 1   DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
           DEE  R++ EK KS +  F + NGTVTAAN+S +NDGAA+++LVS+  L +  L P+A +
Sbjct: 221 DEEPARLHVEKLKSARTVFQRENGTVTAANASPINDGAAAIILVSERVLKEKNLKPLAIV 280

Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICN-KNAEEIAMWEINEAFSVVVLANIKITKGMD 354
            G+ +AA  P DF  AP+ ++ K LK    ++   +  +E NEAFSVV L N KI K +D
Sbjct: 281 KGWGEAAHLPADFTWAPSLAVPKALKHAGIEDINSVDYFEFNEAFSVVGLVNTKILK-LD 339

Query: 355 PSKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKV--NELGVASICNGGGGASSIMIQ 519
           PSKVN++GGAV++GHP+G SG R+V+ L   L+    ++GVA+ICNGGGGASS++I+
Sbjct: 340 PSKVNVYGGAVALGHPLGCSGARVVVTLLSILQQEGGKIGVAAICNGGGGASSVVIE 396

>sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Peroxisomal acetoacetyl-CoA
           thiolase) (Thiolase IA)
          Length = 403

 Score =  151 bits (382), Expect = 1e-36
 Identities = 82/176 (46%), Positives = 118/176 (67%), Gaps = 3/176 (1%)
 Frame = +1

Query: 1   DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
           DEE  + N E+ KS +  F K NGTVTA N+S LNDG A+++LVS+  L +  L P+AKI
Sbjct: 221 DEEIGKFNEERLKSARTVFQKENGTVTAPNASKLNDGGAALVLVSEAKLKQLGLKPLAKI 280

Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
            G+ +AA  P DF IAPA ++ K +K      + +  +E+NEAFSVV LAN ++   +  
Sbjct: 281 SGWGEAARTPFDFTIAPALAVPKAVKHAGLTVDRVDFFELNEAFSVVGLANAELV-NIPL 339

Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNL--KVNELGVASICNGGGGASSIMIQ 519
            K+N++GGAV+MGHP+G SG R+++ L   L  +    GVA +CNGGGGAS+++I+
Sbjct: 340 EKLNVYGGAVAMGHPLGCSGARIIVTLLSVLTQEGGRFGVAGVCNGGGGASAVVIE 395

>sp|Q9FIK7|THIC2_ARATH Probable acetyl-CoA acetyltransferase, cytosolic 2 (Cytosolic
           acetoacetyl-CoA thiolase 2) (Thiolase 2)
          Length = 415

 Score =  151 bits (382), Expect = 1e-36
 Identities = 84/176 (47%), Positives = 121/176 (68%), Gaps = 3/176 (1%)
 Frame = +1

Query: 1   DEEYKRVNFEKFKSLKPAFKANG-TVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
           DE   + +  K K L+P+FK +G +VTA N+S+++DGAA+++LVS E   +  L  +AKI
Sbjct: 229 DEGLGKFDAAKLKKLRPSFKEDGGSVTAGNASSISDGAAALVLVSGEKALELGLHVIAKI 288

Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
            G+ADAA  P  F   PA +I K +K    +A ++  +EINEAFSVV LAN K+  G+DP
Sbjct: 289 RGYADAAQAPELFTTTPALAIPKAIKRAGLDASQVDYYEINEAFSVVALANQKLL-GLDP 347

Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHL--AYNLKVNELGVASICNGGGGASSIMIQ 519
            ++N HGGAVS+GHP+G SG R+++ L      K  + GVASICNGGGGAS+++++
Sbjct: 348 ERLNAHGGAVSLGHPLGCSGARILVTLLGVLRAKKGKYGVASICNGGGGASALVLE 403

>sp|Q04677|THIB_CANTR Acetyl-CoA acetyltransferase IB (Peroxisomal acetoacetyl-CoA
           thiolase) (Thiolase IB)
          Length = 403

 Score =  151 bits (381), Expect = 1e-36
 Identities = 82/176 (46%), Positives = 118/176 (67%), Gaps = 3/176 (1%)
 Frame = +1

Query: 1   DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
           DEE  + N ++ KS +  F K NGTVTA N+S LNDG A+++LVS+  L +  L P+AKI
Sbjct: 221 DEEIGKFNEDRLKSARTVFQKENGTVTAPNASKLNDGGAALVLVSEAKLKQLGLKPLAKI 280

Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
            G+ +AA  P DF IAPA ++ K +K      + +  +E+NEAFSVV LAN ++ K +  
Sbjct: 281 SGWGEAARTPFDFTIAPALAVPKAVKHAGLTVDRVDFFELNEAFSVVGLANAELVK-IPL 339

Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNL--KVNELGVASICNGGGGASSIMIQ 519
            K+N++GGAV+MGHP+G SG R+++ L   L  +    G A +CNGGGGAS+I+I+
Sbjct: 340 EKLNVYGGAVAMGHPLGCSGARIIVTLLSVLTQEGGRFGAAGVCNGGGGASAIVIE 395

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,211,259
Number of Sequences: 369166
Number of extensions: 1200721
Number of successful extensions: 3478
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3296
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3367
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4226258440
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00722

  1. Dr_sW_018_M22
  2. Dr_sW_018_N21