Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_N21
(530 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondr... 207 1e-53
sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochon... 203 2e-52
sp|Q8HXY6|THIL_MACFA Acetyl-CoA acetyltransferase, mitochon... 194 1e-49
sp|P24752|THIL_HUMAN Acetyl-CoA acetyltransferase, mitochon... 193 2e-49
sp|Q6L8K7|THIL_YARLI Acetyl-CoA acetyltransferase (Peroxiso... 152 5e-37
sp|P41338|THIL_YEAST Acetyl-CoA acetyltransferase (Acetoace... 151 8e-37
sp|P10551|THIL_SACBA Acetyl-CoA acetyltransferase (Acetoace... 146 3e-35
sp|Q9FIK7|THIC2_ARATH Probable acetyl-CoA acetyltransferase... 144 1e-34
sp|Q04677|THIB_CANTR Acetyl-CoA acetyltransferase IB (Perox... 142 7e-34
sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Perox... 141 1e-33
>sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 424
Score = 207 bits (527), Expect = 1e-53
Identities = 100/154 (64%), Positives = 129/154 (83%)
Frame = +2
Query: 14 KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKIIGFADAALQPIDFPIAPAES 193
K NGTVTAAN+STLNDGAA+V+L++ EA + K+ P+A+I FADAA+ PIDFP+APA +
Sbjct: 270 KENGTVTAANASTLNDGAAAVVLMTAEAAQRLKVKPLARIAAFADAAVDPIDFPLAPAYA 329
Query: 194 IKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDPSKVNIHGGAVSMGHPIGMSG 373
+ K+LK E+IAMWE+NEAFSVVVLANIK+ + +DP KVN+HGGAVS+GHPIGMSG
Sbjct: 330 VPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKMLE-IDPQKVNVHGGAVSLGHPIGMSG 388
Query: 374 TRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 475
R+V+HLA+ LK E G+ASICNGGGGAS+++I+
Sbjct: 389 ARIVVHLAHALKQGEFGLASICNGGGGASAVLIE 422
>sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 424
Score = 203 bits (516), Expect = 2e-52
Identities = 97/154 (62%), Positives = 128/154 (83%)
Frame = +2
Query: 14 KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKIIGFADAALQPIDFPIAPAES 193
K NGT+TAAN+STLNDGAA+++L++ EA + + P+A+I FADAA+ PIDFP+APA +
Sbjct: 270 KENGTITAANASTLNDGAAALVLMTAEAAQRLNVKPLARIAAFADAAVDPIDFPLAPAYA 329
Query: 194 IKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDPSKVNIHGGAVSMGHPIGMSG 373
+ K+LK E+IAMWE+NEAFSVVVLANIK+ + +DP KVNIHGGAVS+GHPIGMSG
Sbjct: 330 VPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKMLE-IDPQKVNIHGGAVSLGHPIGMSG 388
Query: 374 TRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 475
R+V+H+A+ LK E G+ASICNGGGGAS+++I+
Sbjct: 389 ARIVVHMAHALKPGEFGLASICNGGGGASALLIE 422
>sp|Q8HXY6|THIL_MACFA Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 427
Score = 194 bits (492), Expect = 1e-49
Identities = 94/154 (61%), Positives = 125/154 (81%)
Frame = +2
Query: 14 KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKIIGFADAALQPIDFPIAPAES 193
K NGT+TAAN+STLNDGAA+++L++ A + + P+A+I+ FADAA++PIDFPIAP +
Sbjct: 273 KENGTITAANASTLNDGAAALVLMTAGAAKRLNVTPLARIVAFADAAVEPIDFPIAPVHA 332
Query: 194 IKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDPSKVNIHGGAVSMGHPIGMSG 373
+ +LK E+IAMWE+NEAFS+VVLANIK+ + +DP KVNI+GGAVS+GHPIGMSG
Sbjct: 333 VSMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLE-IDPQKVNINGGAVSLGHPIGMSG 391
Query: 374 TRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 475
R+V HL + LK E G+ASICNGGGGAS+++IQ
Sbjct: 392 ARIVGHLTHALKQGEYGLASICNGGGGASAMLIQ 425
>sp|P24752|THIL_HUMAN Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase) (T2)
Length = 427
Score = 193 bits (491), Expect = 2e-49
Identities = 95/154 (61%), Positives = 125/154 (81%)
Frame = +2
Query: 14 KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKIIGFADAALQPIDFPIAPAES 193
K NGTVTAAN+STLNDGAA+++L++ +A + + P+A+I+ FADAA++PIDFPIAP +
Sbjct: 273 KENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYA 332
Query: 194 IKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDPSKVNIHGGAVSMGHPIGMSG 373
+LK E+IAMWE+NEAFS+VVLANIK+ + +DP KVNI+GGAVS+GHPIGMSG
Sbjct: 333 ASMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLE-IDPQKVNINGGAVSLGHPIGMSG 391
Query: 374 TRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 475
R+V HL + LK E G+ASICNGGGGAS+++IQ
Sbjct: 392 ARIVGHLTHALKQGEYGLASICNGGGGASAMLIQ 425
>sp|Q6L8K7|THIL_YARLI Acetyl-CoA acetyltransferase (Peroxisomal acetoacetyl-CoA thiolase)
(Thiolase)
Length = 397
Score = 152 bits (384), Expect = 5e-37
Identities = 79/158 (50%), Positives = 109/158 (68%)
Frame = +2
Query: 2 KPAFKANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKIIGFADAALQPIDFPIA 181
KP F G+VTA N+S +NDGA++V++ S + +AKI+ +ADAA PIDF IA
Sbjct: 235 KPVFDKQGSVTAGNASPINDGASAVVVASGTKAKELGTPVLAKIVSYADAATAPIDFTIA 294
Query: 182 PAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDPSKVNIHGGAVSMGHPI 361
P+ +I LK ++IA+WEINEAFS V LAN+ + G+D SKVN+ GGAV++GHPI
Sbjct: 295 PSLAIPAALKKAGLTKDDIALWEINEAFSGVALANL-MRLGIDKSKVNVKGGAVALGHPI 353
Query: 362 GMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 475
G SG R+ + L LK E GVA+ICNGGG +++I+I+
Sbjct: 354 GASGNRIFVTLVNALKEGEYGVAAICNGGGASTAIVIK 391
>sp|P41338|THIL_YEAST Acetyl-CoA acetyltransferase (Acetoacetyl-CoA thiolase)
Length = 398
Score = 151 bits (382), Expect = 8e-37
Identities = 84/157 (53%), Positives = 115/157 (73%), Gaps = 3/157 (1%)
Frame = +2
Query: 14 KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKIIGFADAALQPIDFPIAPAES 193
K NGTVTAAN+S +NDGAA+V+LVS++ L + L P+A I G+ +AA QP DF AP+ +
Sbjct: 241 KENGTVTAANASPINDGAAAVILVSEKVLKEKNLKPLAIIKGWGEAAHQPADFTWAPSLA 300
Query: 194 IKKLLKICN-KNAEEIAMWEINEAFSVVVLANIKITKGMDPSKVNIHGGAVSMGHPIGMS 370
+ K LK ++ + +E NEAFSVV L N KI K +DPSKVN++GGAV++GHP+G S
Sbjct: 301 VPKALKHAGIEDINSVDYFEFNEAFSVVGLVNTKILK-LDPSKVNVYGGAVALGHPLGCS 359
Query: 371 GTRLVLHLAYNLKV--NELGVASICNGGGGASSIMIQ 475
G R+V+ L L+ ++GVA+ICNGGGGASSI+I+
Sbjct: 360 GARVVVTLLSILQQEGGKIGVAAICNGGGGASSIVIE 396
>sp|P10551|THIL_SACBA Acetyl-CoA acetyltransferase (Acetoacetyl-CoA thiolase)
Length = 398
Score = 146 bits (368), Expect = 3e-35
Identities = 79/157 (50%), Positives = 113/157 (71%), Gaps = 3/157 (1%)
Frame = +2
Query: 14 KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKIIGFADAALQPIDFPIAPAES 193
+ NGTVTAAN+S +NDGAA+++LVS+ L + L P+A + G+ +AA P DF AP+ +
Sbjct: 241 RENGTVTAANASPINDGAAAIILVSERVLKEKNLKPLAIVKGWGEAAHLPADFTWAPSLA 300
Query: 194 IKKLLKICN-KNAEEIAMWEINEAFSVVVLANIKITKGMDPSKVNIHGGAVSMGHPIGMS 370
+ K LK ++ + +E NEAFSVV L N KI K +DPSKVN++GGAV++GHP+G S
Sbjct: 301 VPKALKHAGIEDINSVDYFEFNEAFSVVGLVNTKILK-LDPSKVNVYGGAVALGHPLGCS 359
Query: 371 GTRLVLHLAYNLKV--NELGVASICNGGGGASSIMIQ 475
G R+V+ L L+ ++GVA+ICNGGGGASS++I+
Sbjct: 360 GARVVVTLLSILQQEGGKIGVAAICNGGGGASSVVIE 396
>sp|Q9FIK7|THIC2_ARATH Probable acetyl-CoA acetyltransferase, cytosolic 2 (Cytosolic
acetoacetyl-CoA thiolase 2) (Thiolase 2)
Length = 415
Score = 144 bits (363), Expect = 1e-34
Identities = 79/161 (49%), Positives = 114/161 (70%), Gaps = 3/161 (1%)
Frame = +2
Query: 2 KPAFKANG-TVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKIIGFADAALQPIDFPI 178
+P+FK +G +VTA N+S+++DGAA+++LVS E + L +AKI G+ADAA P F
Sbjct: 244 RPSFKEDGGSVTAGNASSISDGAAALVLVSGEKALELGLHVIAKIRGYADAAQAPELFTT 303
Query: 179 APAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDPSKVNIHGGAVSMGHP 358
PA +I K +K +A ++ +EINEAFSVV LAN K+ G+DP ++N HGGAVS+GHP
Sbjct: 304 TPALAIPKAIKRAGLDASQVDYYEINEAFSVVALANQKLL-GLDPERLNAHGGAVSLGHP 362
Query: 359 IGMSGTRLVLHL--AYNLKVNELGVASICNGGGGASSIMIQ 475
+G SG R+++ L K + GVASICNGGGGAS+++++
Sbjct: 363 LGCSGARILVTLLGVLRAKKGKYGVASICNGGGGASALVLE 403
>sp|Q04677|THIB_CANTR Acetyl-CoA acetyltransferase IB (Peroxisomal acetoacetyl-CoA
thiolase) (Thiolase IB)
Length = 403
Score = 142 bits (357), Expect = 7e-34
Identities = 75/156 (48%), Positives = 107/156 (68%), Gaps = 2/156 (1%)
Frame = +2
Query: 14 KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKIIGFADAALQPIDFPIAPAES 193
K NGTVTA N+S LNDG A+++LVS+ L + L P+AKI G+ +AA P DF IAPA +
Sbjct: 241 KENGTVTAPNASKLNDGGAALVLVSEAKLKQLGLKPLAKISGWGEAARTPFDFTIAPALA 300
Query: 194 IKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDPSKVNIHGGAVSMGHPIGMSG 373
+ K +K + + +E+NEAFSVV LAN ++ K + K+N++GGAV+MGHP+G SG
Sbjct: 301 VPKAVKHAGLTVDRVDFFELNEAFSVVGLANAELVK-IPLEKLNVYGGAVAMGHPLGCSG 359
Query: 374 TRLVLHLAYNL--KVNELGVASICNGGGGASSIMIQ 475
R+++ L L + G A +CNGGGGAS+I+I+
Sbjct: 360 ARIIVTLLSVLTQEGGRFGAAGVCNGGGGASAIVIE 395
>sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Peroxisomal acetoacetyl-CoA
thiolase) (Thiolase IA)
Length = 403
Score = 141 bits (355), Expect = 1e-33
Identities = 74/156 (47%), Positives = 107/156 (68%), Gaps = 2/156 (1%)
Frame = +2
Query: 14 KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKIIGFADAALQPIDFPIAPAES 193
K NGTVTA N+S LNDG A+++LVS+ L + L P+AKI G+ +AA P DF IAPA +
Sbjct: 241 KENGTVTAPNASKLNDGGAALVLVSEAKLKQLGLKPLAKISGWGEAARTPFDFTIAPALA 300
Query: 194 IKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDPSKVNIHGGAVSMGHPIGMSG 373
+ K +K + + +E+NEAFSVV LAN ++ + K+N++GGAV+MGHP+G SG
Sbjct: 301 VPKAVKHAGLTVDRVDFFELNEAFSVVGLANAELV-NIPLEKLNVYGGAVAMGHPLGCSG 359
Query: 374 TRLVLHLAYNL--KVNELGVASICNGGGGASSIMIQ 475
R+++ L L + GVA +CNGGGGAS+++I+
Sbjct: 360 ARIIVTLLSVLTQEGGRFGVAGVCNGGGGASAVVIE 395
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,758,817
Number of Sequences: 369166
Number of extensions: 1116326
Number of successful extensions: 3044
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2886
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2941
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3600891075
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)