Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_M22
(574 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondr... 226 3e-59
sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochon... 222 6e-58
sp|Q8HXY6|THIL_MACFA Acetyl-CoA acetyltransferase, mitochon... 213 4e-55
sp|P24752|THIL_HUMAN Acetyl-CoA acetyltransferase, mitochon... 212 5e-55
sp|Q6L8K7|THIL_YARLI Acetyl-CoA acetyltransferase (Peroxiso... 167 2e-41
sp|P41338|THIL_YEAST Acetyl-CoA acetyltransferase (Acetoace... 162 6e-40
sp|P10551|THIL_SACBA Acetyl-CoA acetyltransferase (Acetoace... 158 1e-38
sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Perox... 151 1e-36
sp|Q9FIK7|THIC2_ARATH Probable acetyl-CoA acetyltransferase... 151 1e-36
sp|Q04677|THIB_CANTR Acetyl-CoA acetyltransferase IB (Perox... 151 1e-36
>sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 424
Score = 226 bits (576), Expect = 3e-59
Identities = 112/174 (64%), Positives = 142/174 (81%), Gaps = 1/174 (0%)
Frame = +1
Query: 1 DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
DEEYKRV+F K LK F K NGTVTAAN+STLNDGAA+V+L++ EA + K+ P+A+I
Sbjct: 250 DEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAAVVLMTAEAAQRLKVKPLARI 309
Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
FADAA+ PIDFP+APA ++ K+LK E+IAMWE+NEAFSVVVLANIK+ + +DP
Sbjct: 310 AAFADAAVDPIDFPLAPAYAVPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKMLE-IDP 368
Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 519
KVN+HGGAVS+GHPIGMSG R+V+HLA+ LK E G+ASICNGGGGAS+++I+
Sbjct: 369 QKVNVHGGAVSLGHPIGMSGARIVVHLAHALKQGEFGLASICNGGGGASAVLIE 422
>sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 424
Score = 222 bits (565), Expect = 6e-58
Identities = 109/174 (62%), Positives = 141/174 (81%), Gaps = 1/174 (0%)
Frame = +1
Query: 1 DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
DEEYKRV+F K LK F K NGT+TAAN+STLNDGAA+++L++ EA + + P+A+I
Sbjct: 250 DEEYKRVDFSKVPKLKTVFQKENGTITAANASTLNDGAAALVLMTAEAAQRLNVKPLARI 309
Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
FADAA+ PIDFP+APA ++ K+LK E+IAMWE+NEAFSVVVLANIK+ + +DP
Sbjct: 310 AAFADAAVDPIDFPLAPAYAVPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKMLE-IDP 368
Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 519
KVNIHGGAVS+GHPIGMSG R+V+H+A+ LK E G+ASICNGGGGAS+++I+
Sbjct: 369 QKVNIHGGAVSLGHPIGMSGARIVVHMAHALKPGEFGLASICNGGGGASALLIE 422
>sp|Q8HXY6|THIL_MACFA Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 427
Score = 213 bits (541), Expect = 4e-55
Identities = 106/174 (60%), Positives = 138/174 (79%), Gaps = 1/174 (0%)
Frame = +1
Query: 1 DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
DEEYKRV+F K LK F K NGT+TAAN+STLNDGAA+++L++ A + + P+A+I
Sbjct: 253 DEEYKRVDFSKVPKLKTVFQKENGTITAANASTLNDGAAALVLMTAGAAKRLNVTPLARI 312
Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
+ FADAA++PIDFPIAP ++ +LK E+IAMWE+NEAFS+VVLANIK+ + +DP
Sbjct: 313 VAFADAAVEPIDFPIAPVHAVSMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLE-IDP 371
Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 519
KVNI+GGAVS+GHPIGMSG R+V HL + LK E G+ASICNGGGGAS+++IQ
Sbjct: 372 QKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQ 425
>sp|P24752|THIL_HUMAN Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase) (T2)
Length = 427
Score = 212 bits (540), Expect = 5e-55
Identities = 107/174 (61%), Positives = 138/174 (79%), Gaps = 1/174 (0%)
Frame = +1
Query: 1 DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
DEEYKRV+F K LK F K NGTVTAAN+STLNDGAA+++L++ +A + + P+A+I
Sbjct: 253 DEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARI 312
Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
+ FADAA++PIDFPIAP + +LK E+IAMWE+NEAFS+VVLANIK+ + +DP
Sbjct: 313 VAFADAAVEPIDFPIAPVYAASMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLE-IDP 371
Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 519
KVNI+GGAVS+GHPIGMSG R+V HL + LK E G+ASICNGGGGAS+++IQ
Sbjct: 372 QKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQ 425
>sp|Q6L8K7|THIL_YARLI Acetyl-CoA acetyltransferase (Peroxisomal acetoacetyl-CoA thiolase)
(Thiolase)
Length = 397
Score = 167 bits (423), Expect = 2e-41
Identities = 88/173 (50%), Positives = 118/173 (68%)
Frame = +1
Query: 1 DEEYKRVNFEKFKSLKPAFKANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKII 180
DEE K N EK K LKP F G+VTA N+S +NDGA++V++ S + +AKI+
Sbjct: 220 DEEPKGANAEKLKGLKPVFDKQGSVTAGNASPINDGASAVVVASGTKAKELGTPVLAKIV 279
Query: 181 GFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDPS 360
+ADAA PIDF IAP+ +I LK ++IA+WEINEAFS V LAN+ + G+D S
Sbjct: 280 SYADAATAPIDFTIAPSLAIPAALKKAGLTKDDIALWEINEAFSGVALANL-MRLGIDKS 338
Query: 361 KVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKVNELGVASICNGGGGASSIMIQ 519
KVN+ GGAV++GHPIG SG R+ + L LK E GVA+ICNGGG +++I+I+
Sbjct: 339 KVNVKGGAVALGHPIGASGNRIFVTLVNALKEGEYGVAAICNGGGASTAIVIK 391
>sp|P41338|THIL_YEAST Acetyl-CoA acetyltransferase (Acetoacetyl-CoA thiolase)
Length = 398
Score = 162 bits (410), Expect = 6e-40
Identities = 92/177 (51%), Positives = 127/177 (71%), Gaps = 4/177 (2%)
Frame = +1
Query: 1 DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
DEE R++ EK +S + F K NGTVTAAN+S +NDGAA+V+LVS++ L + L P+A I
Sbjct: 221 DEEPARLHVEKLRSARTVFQKENGTVTAANASPINDGAAAVILVSEKVLKEKNLKPLAII 280
Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICN-KNAEEIAMWEINEAFSVVVLANIKITKGMD 354
G+ +AA QP DF AP+ ++ K LK ++ + +E NEAFSVV L N KI K +D
Sbjct: 281 KGWGEAAHQPADFTWAPSLAVPKALKHAGIEDINSVDYFEFNEAFSVVGLVNTKILK-LD 339
Query: 355 PSKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKV--NELGVASICNGGGGASSIMIQ 519
PSKVN++GGAV++GHP+G SG R+V+ L L+ ++GVA+ICNGGGGASSI+I+
Sbjct: 340 PSKVNVYGGAVALGHPLGCSGARVVVTLLSILQQEGGKIGVAAICNGGGGASSIVIE 396
>sp|P10551|THIL_SACBA Acetyl-CoA acetyltransferase (Acetoacetyl-CoA thiolase)
Length = 398
Score = 158 bits (399), Expect = 1e-38
Identities = 88/177 (49%), Positives = 125/177 (70%), Gaps = 4/177 (2%)
Frame = +1
Query: 1 DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
DEE R++ EK KS + F + NGTVTAAN+S +NDGAA+++LVS+ L + L P+A +
Sbjct: 221 DEEPARLHVEKLKSARTVFQRENGTVTAANASPINDGAAAIILVSERVLKEKNLKPLAIV 280
Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICN-KNAEEIAMWEINEAFSVVVLANIKITKGMD 354
G+ +AA P DF AP+ ++ K LK ++ + +E NEAFSVV L N KI K +D
Sbjct: 281 KGWGEAAHLPADFTWAPSLAVPKALKHAGIEDINSVDYFEFNEAFSVVGLVNTKILK-LD 339
Query: 355 PSKVNIHGGAVSMGHPIGMSGTRLVLHLAYNLKV--NELGVASICNGGGGASSIMIQ 519
PSKVN++GGAV++GHP+G SG R+V+ L L+ ++GVA+ICNGGGGASS++I+
Sbjct: 340 PSKVNVYGGAVALGHPLGCSGARVVVTLLSILQQEGGKIGVAAICNGGGGASSVVIE 396
>sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Peroxisomal acetoacetyl-CoA
thiolase) (Thiolase IA)
Length = 403
Score = 151 bits (382), Expect = 1e-36
Identities = 82/176 (46%), Positives = 118/176 (67%), Gaps = 3/176 (1%)
Frame = +1
Query: 1 DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
DEE + N E+ KS + F K NGTVTA N+S LNDG A+++LVS+ L + L P+AKI
Sbjct: 221 DEEIGKFNEERLKSARTVFQKENGTVTAPNASKLNDGGAALVLVSEAKLKQLGLKPLAKI 280
Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
G+ +AA P DF IAPA ++ K +K + + +E+NEAFSVV LAN ++ +
Sbjct: 281 SGWGEAARTPFDFTIAPALAVPKAVKHAGLTVDRVDFFELNEAFSVVGLANAELV-NIPL 339
Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNL--KVNELGVASICNGGGGASSIMIQ 519
K+N++GGAV+MGHP+G SG R+++ L L + GVA +CNGGGGAS+++I+
Sbjct: 340 EKLNVYGGAVAMGHPLGCSGARIIVTLLSVLTQEGGRFGVAGVCNGGGGASAVVIE 395
>sp|Q9FIK7|THIC2_ARATH Probable acetyl-CoA acetyltransferase, cytosolic 2 (Cytosolic
acetoacetyl-CoA thiolase 2) (Thiolase 2)
Length = 415
Score = 151 bits (382), Expect = 1e-36
Identities = 84/176 (47%), Positives = 121/176 (68%), Gaps = 3/176 (1%)
Frame = +1
Query: 1 DEEYKRVNFEKFKSLKPAFKANG-TVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
DE + + K K L+P+FK +G +VTA N+S+++DGAA+++LVS E + L +AKI
Sbjct: 229 DEGLGKFDAAKLKKLRPSFKEDGGSVTAGNASSISDGAAALVLVSGEKALELGLHVIAKI 288
Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
G+ADAA P F PA +I K +K +A ++ +EINEAFSVV LAN K+ G+DP
Sbjct: 289 RGYADAAQAPELFTTTPALAIPKAIKRAGLDASQVDYYEINEAFSVVALANQKLL-GLDP 347
Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHL--AYNLKVNELGVASICNGGGGASSIMIQ 519
++N HGGAVS+GHP+G SG R+++ L K + GVASICNGGGGAS+++++
Sbjct: 348 ERLNAHGGAVSLGHPLGCSGARILVTLLGVLRAKKGKYGVASICNGGGGASALVLE 403
>sp|Q04677|THIB_CANTR Acetyl-CoA acetyltransferase IB (Peroxisomal acetoacetyl-CoA
thiolase) (Thiolase IB)
Length = 403
Score = 151 bits (381), Expect = 1e-36
Identities = 82/176 (46%), Positives = 118/176 (67%), Gaps = 3/176 (1%)
Frame = +1
Query: 1 DEEYKRVNFEKFKSLKPAF-KANGTVTAANSSTLNDGAASVLLVSKEALDKYKLIPMAKI 177
DEE + N ++ KS + F K NGTVTA N+S LNDG A+++LVS+ L + L P+AKI
Sbjct: 221 DEEIGKFNEDRLKSARTVFQKENGTVTAPNASKLNDGGAALVLVSEAKLKQLGLKPLAKI 280
Query: 178 IGFADAALQPIDFPIAPAESIKKLLKICNKNAEEIAMWEINEAFSVVVLANIKITKGMDP 357
G+ +AA P DF IAPA ++ K +K + + +E+NEAFSVV LAN ++ K +
Sbjct: 281 SGWGEAARTPFDFTIAPALAVPKAVKHAGLTVDRVDFFELNEAFSVVGLANAELVK-IPL 339
Query: 358 SKVNIHGGAVSMGHPIGMSGTRLVLHLAYNL--KVNELGVASICNGGGGASSIMIQ 519
K+N++GGAV+MGHP+G SG R+++ L L + G A +CNGGGGAS+I+I+
Sbjct: 340 EKLNVYGGAVAMGHPLGCSGARIIVTLLSVLTQEGGRFGAAGVCNGGGGASAIVIE 395
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,211,259
Number of Sequences: 369166
Number of extensions: 1200721
Number of successful extensions: 3478
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3296
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3367
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4226258440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)