Planaria EST Database

DrC_00710

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00710
         (943 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q13825|AUMH_HUMAN  Methylglutaconyl-CoA hydratase, mitoch...   229   1e-59
sp|Q9JLZ3|AUHM_MOUSE  Methylglutaconyl-CoA hydratase, mitoch...   223   6e-58
sp|P52046|CRT_CLOAB  3-hydroxybutyryl-CoA dehydratase (Croto...   142   2e-33
sp|P64017|ECHA8_MYCBO  Probable enoyl-CoA hydratase echA8 >g...   124   4e-28
sp|O07137|ECHA8_MYCLE  Probable enoyl-CoA hydratase echA8         123   7e-28
sp|P34559|ECHM_CAEEL  Probable enoyl-CoA hydratase, mitochon...   120   4e-27
sp|P14604|ECHM_RAT  Enoyl-CoA hydratase, mitochondrial precu...   117   6e-26
sp|Q58DM8|ECHM_BOVIN  Enoyl-CoA hydratase, mitochondrial pre...   117   6e-26
sp|P76082|PAAF_ECOLI  Probable enoyl-CoA hydratase paaF           114   4e-25
sp|P30084|ECHM_HUMAN  Enoyl-CoA hydratase, mitochondrial pre...   113   7e-25

>sp|Q13825|AUMH_HUMAN Methylglutaconyl-CoA hydratase, mitochondrial precursor
           (AU-specific RNA-binding enoyl-CoA hydratase)
           (AU-binding protein/enoyl-CoA hydratase)
          Length = 339

 Score =  229 bits (583), Expect = 1e-59
 Identities = 118/249 (47%), Positives = 165/249 (66%)
 Frame = +2

Query: 110 IGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKERRHMPE 289
           +G+NR   KNSL KNL+K    AV+ L   K +R ++I + VP IFCAGADLKER  M  
Sbjct: 92  LGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAGADLKERAKMSS 151

Query: 290 SDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGLVETG 469
           S++  FV   R +   +A +P+PTI AID             CD+R ++ +  MGLVET 
Sbjct: 152 SEVGPFVSKIRAVINDIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETK 211

Query: 470 LAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSALLHA 649
           LAIIPG GGTQRL R IG++ AKELIF+A+ + GKEA+ +G+++  ++QN +G++A   A
Sbjct: 212 LAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKA 271

Query: 650 FTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLKAFQE 829
             +A+     GP+ +++AK AI+ G+ V  V   L +EE CYAQ IPT+DRLEGL AF+E
Sbjct: 272 LDLAREFLPQGPVAMRVAKLAINQGMEVDLV-TGLAIEEACYAQTIPTKDRLEGLLAFKE 330

Query: 830 KRKPKYEGK 856
           KR P+Y+G+
Sbjct: 331 KRPPRYKGE 339

>sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitochondrial precursor
           (AU-specific RNA-binding enoyl-CoA hydratase)
           (AU-binding enoyl-CoA hydratase) (muAUH)
          Length = 314

 Score =  223 bits (568), Expect = 6e-58
 Identities = 115/249 (46%), Positives = 165/249 (66%)
 Frame = +2

Query: 110 IGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKERRHMPE 289
           +G+NR   KN+L KNL+K    AV+ L   K +R ++I + VP IFCAGADLKER  M  
Sbjct: 67  LGINRAYGKNALSKNLLKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAGADLKERAKMHS 126

Query: 290 SDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGLVETG 469
           S++  FV   R++   +A +P+PTI AID             CD+R ++ +  MGLVET 
Sbjct: 127 SEVGPFVSKIRSVINDIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETK 186

Query: 470 LAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSALLHA 649
           LAIIPG GGTQRL R IG++ AKELIF+A+ + G+EA+ +G+++  ++QN +G++A   A
Sbjct: 187 LAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGQEAKAVGLISHVLEQNQEGDAAYRKA 246

Query: 650 FTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLKAFQE 829
             +A+     GP+ +++AK AI+ G+ V  V   L +EE CYAQ I T+DRLEGL AF+E
Sbjct: 247 LDLAREFLPQGPVAMRVAKLAINQGMEVDLV-TGLAIEEACYAQTISTKDRLEGLLAFKE 305

Query: 830 KRKPKYEGK 856
           KR P+Y+G+
Sbjct: 306 KRPPRYKGE 314

>sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Crotonase)
          Length = 261

 Score =  142 bits (357), Expect = 2e-33
 Identities = 82/247 (33%), Positives = 131/247 (53%)
 Frame = +2

Query: 98  KIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKERR 277
           K+  + +NRPK+ N+L  + +K+ +  +  +    ++  V++       F AGAD+ E +
Sbjct: 13  KVAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSFVAGADISEMK 72

Query: 278 HMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGL 457
            M   +   F      +F+ +  +  P I A++             CD+R +S N   G 
Sbjct: 73  EMNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDIRIASSNARFGQ 132

Query: 458 VETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSA 637
            E GL I PG GGTQRLSRL+G+  AK+LIFTA+ I   EA  IG+VN  V+ +   N+A
Sbjct: 133 PEVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNKVVEPSELMNTA 192

Query: 638 LLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLK 817
                 +A  I +N P+ VK++K AI+ G++   +  AL  E + + +   TED+ + + 
Sbjct: 193 K----EIANKIVSNAPVAVKLSKQAINRGMQ-CDIDTALAFESEAFGECFSTEDQKDAMT 247

Query: 818 AFQEKRK 838
           AF EKRK
Sbjct: 248 AFIEKRK 254

>sp|P64017|ECHA8_MYCBO Probable enoyl-CoA hydratase echA8
 sp|P64016|ECHA8_MYCTU Probable enoyl-CoA hydratase echA8
          Length = 257

 Score =  124 bits (311), Expect = 4e-28
 Identities = 83/263 (31%), Positives = 134/263 (50%)
 Frame = +2

Query: 59  TEKEIIFDTDHPSKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VP 238
           T + I+ + D   ++G I LNRP++ N+L   ++ +  +A   L +  DI  ++I     
Sbjct: 2   TYETILVERDQ--RVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITG-SA 58

Query: 239 NIFCAGADLKERRHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXC 418
             F AGAD+KE   +  +D   F       +  +A +  PTI A+              C
Sbjct: 59  KAFAAGADIKEMADLTFADA--FTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMC 116

Query: 419 DLRYSSENTFMGLVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIV 598
           D+  +++    G  E  L ++PG GG+QRL+R IG AKA +LI T +T+   EAE  G+V
Sbjct: 117 DVLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLV 176

Query: 599 NACVKQNPDGNSALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYA 778
           +  V  +     A   A T++QM ++      +MAK A++     SS+ + LL E + + 
Sbjct: 177 SRVVPADDLLTEARATATTISQMSAS----AARMAKEAVNRAFE-SSLSEGLLYERRLFH 231

Query: 779 QIIPTEDRLEGLKAFQEKRKPKY 847
               TED+ EG+ AF EKR P++
Sbjct: 232 SAFATEDQSEGMAAFIEKRAPQF 254

>sp|O07137|ECHA8_MYCLE Probable enoyl-CoA hydratase echA8
          Length = 257

 Score =  123 bits (309), Expect = 7e-28
 Identities = 82/254 (32%), Positives = 127/254 (50%)
 Frame = +2

Query: 86  DHPSKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADL 265
           D   ++G I LNRP++ N+L   ++ +  NA   L    D+  ++I    P +F AGAD+
Sbjct: 9   DGYQRVGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITG-SPKVFAAGADI 67

Query: 266 KERRHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENT 445
           KE   +  +D   F     + +  +A +  P I A+              CDL  +++  
Sbjct: 68  KEMASLTFTDA--FDADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADTA 125

Query: 446 FMGLVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPD 625
             G  E  L ++PG GG+QRL+R IG AKA +LI T +TI   EAE  G+V+  V     
Sbjct: 126 KFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRVVL---- 181

Query: 626 GNSALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRL 805
            +  L  A  VA  IS       +MAK A++     S++ + LL E + +     T+D+ 
Sbjct: 182 ADDLLPEAKAVATTISQMSRSATRMAKEAVNRSFE-STLAEGLLHERRLFHSTFVTDDQS 240

Query: 806 EGLKAFQEKRKPKY 847
           EG+ AF EKR P++
Sbjct: 241 EGMAAFIEKRAPQF 254

>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
          Length = 288

 Score =  120 bits (302), Expect = 4e-27
 Identities = 78/253 (30%), Positives = 130/253 (51%), Gaps = 1/253 (0%)
 Frame = +2

Query: 101 IGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKERRH 280
           +  I LNRPK+ N+L   L+ +  +A+ +L   K +  +VI       F AGAD+KE   
Sbjct: 45  VALIKLNRPKALNALCAQLMTELADALEVLDTDKSVGAIVITG-SERAFAAGADIKE--- 100

Query: 281 MPESDLENFVKGA-RNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGL 457
           M  ++      G+  + +  V+++  P I A++             CD+ Y+ E    G 
Sbjct: 101 MTNNEFATTFSGSFLSNWTAVSDVKKPVIAAVNGFALGGGNELAMMCDIIYAGEKARFGQ 160

Query: 458 VETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSA 637
            E  +  IPGAGGTQR +R  G + A E+  T   +T +EA+E GIV+         +  
Sbjct: 161 PEINIGTIPGAGGTQRWARAAGKSFAMEVCLTGNHVTAQEAKEHGIVSKIF----PADQV 216

Query: 638 LLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLK 817
           +  A  + + I++  P+ V+MAK A++    + ++++ L  E + +     T+DR EG+ 
Sbjct: 217 VGEAVKLGEKIADQSPLIVQMAKEAVNKAYEL-TLQEGLHFERRLFHATFATKDRKEGMT 275

Query: 818 AFQEKRKPKYEGK 856
           AF EKRKP++E K
Sbjct: 276 AFAEKRKPQWESK 288

>sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score =  117 bits (292), Expect = 6e-26
 Identities = 75/252 (29%), Positives = 126/252 (50%)
 Frame = +2

Query: 95  SKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKER 274
           S +G I LNRPK+ N+L   L+++   A+    E   +  +V+       F AGAD+KE 
Sbjct: 45  SSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAFAAGADIKEM 103

Query: 275 RHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 454
           ++    D   +     + +  +  I  P I A++             CD+ Y+ E    G
Sbjct: 104 QNRTFQDC--YSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFG 161

Query: 455 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 634
             E  L  IPGAGGTQRL+R +G + A E++ T   I+ ++A++ G+V+          +
Sbjct: 162 QPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPV----ET 217

Query: 635 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 814
            +  A   A+ I+NN  I V MAK +++    + ++ +   +E+K +     T+DR EG+
Sbjct: 218 LVEEAIQCAEKIANNSKIIVAMAKESVNAAFEM-TLTEGNKLEKKLFYSTFATDDRREGM 276

Query: 815 KAFQEKRKPKYE 850
            AF EKRK  ++
Sbjct: 277 SAFVEKRKANFK 288

>sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score =  117 bits (292), Expect = 6e-26
 Identities = 72/252 (28%), Positives = 127/252 (50%)
 Frame = +2

Query: 95  SKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKER 274
           S +G I LNRPK+ N+L   L+ +   A+    E   +  +V+      +F AGAD+KE 
Sbjct: 45  SNVGLIQLNRPKALNALCNGLIVELNQALQAFEEDPAVGAIVLTG-GEKVFAAGADIKEM 103

Query: 275 RHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 454
           + +   +   +  G  + +  +  +  P I A++             CD+ Y+ E    G
Sbjct: 104 QSLTFQNC--YSGGFLSHWDQLTRVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFG 161

Query: 455 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 634
             E  +  IPGAGGTQRL+R +G + A E++ T   I+ ++A++ G+V+          +
Sbjct: 162 QPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPV----ET 217

Query: 635 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 814
            +  A   A+ I++N  I   MAK +++    + ++ + + +E+K +     TEDR EG+
Sbjct: 218 VVEEAIQCAEKIASNSKIVTAMAKESVNAAFEM-TLAEGVKLEKKLFYSTFATEDRKEGM 276

Query: 815 KAFQEKRKPKYE 850
            AF EKRK  ++
Sbjct: 277 AAFVEKRKANFK 288

>sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF
          Length = 255

 Score =  114 bits (285), Expect = 4e-25
 Identities = 77/255 (30%), Positives = 129/255 (50%), Gaps = 2/255 (0%)
 Frame = +2

Query: 98  KIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIR-CVVIGN*VPNIFCAGADLKER 274
           ++  + LNRP ++N+L   L+    N +        I  CV+ GN     F AGADL E 
Sbjct: 11  RVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGN--ARFFAAGADLNE- 67

Query: 275 RHMPESDLENFVKGAR-NLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFM 451
             M E DL   +   R  L+  +     P I A++             CD+  + EN   
Sbjct: 68  --MAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARF 125

Query: 452 GLVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGN 631
           GL E  L I+PGAGGTQRL R +G + A +++ + ++IT ++A++ G+V+         +
Sbjct: 126 GLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVF----PSD 181

Query: 632 SALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEG 811
             L +A  +A  ++ + P+ ++ AK A+     V +++  L  E + +  +  TEDR EG
Sbjct: 182 LTLEYALQLASKMARHSPLALQAAKQALRQSQEV-ALQAGLAQERQLFTLLAATEDRHEG 240

Query: 812 LKAFQEKRKPKYEGK 856
           + AF +KR P ++G+
Sbjct: 241 ISAFLQKRTPDFKGR 255

>sp|P30084|ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score =  113 bits (283), Expect = 7e-25
 Identities = 75/252 (29%), Positives = 126/252 (50%), Gaps = 2/252 (0%)
 Frame = +2

Query: 101 IGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKERRH 280
           +G I LNRPK+ N+L   L+ +   A+    E   +  +V+       F AGAD+KE ++
Sbjct: 47  VGLIQLNRPKALNALCDGLIDELNQALKTFEEDPAVGAIVLTG-GDKAFAAGADIKEMQN 105

Query: 281 MPESDL--ENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 454
           +   D     F+K   +L Q    +  P I A++             CD+ Y+ E     
Sbjct: 106 LSFQDCYSSKFLKHWDHLTQ----VKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFA 161

Query: 455 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 634
             E  +  IPGAGGTQRL+R +G + A E++ T   I+ ++A++ G+V+          +
Sbjct: 162 QPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPV----ET 217

Query: 635 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 814
            +  A   A+ I++N  I V MAK +++    ++  + + L E+K +     T+DR EG+
Sbjct: 218 LVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKL-EKKLFYSTFATDDRKEGM 276

Query: 815 KAFQEKRKPKYE 850
            AF EKRK  ++
Sbjct: 277 TAFVEKRKANFK 288

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 103,864,981
Number of Sequences: 369166
Number of extensions: 2157189
Number of successful extensions: 6218
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5964
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6164
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9750928390
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00710

  1. Dr_sW_010_P10
  2. Dr_sW_018_I16