Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_I16
(943 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13825|AUMH_HUMAN Methylglutaconyl-CoA hydratase, mitoch... 229 1e-59
sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitoch... 223 6e-58
sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Croto... 142 2e-33
sp|P64017|ECHA8_MYCBO Probable enoyl-CoA hydratase echA8 >g... 124 5e-28
sp|O07137|ECHA8_MYCLE Probable enoyl-CoA hydratase echA8 123 7e-28
sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochon... 120 4e-27
sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precu... 117 6e-26
sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial pre... 117 6e-26
sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF 114 4e-25
sp|P30084|ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial pre... 113 7e-25
>sp|Q13825|AUMH_HUMAN Methylglutaconyl-CoA hydratase, mitochondrial precursor
(AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding protein/enoyl-CoA hydratase)
Length = 339
Score = 229 bits (583), Expect = 1e-59
Identities = 118/249 (47%), Positives = 165/249 (66%)
Frame = +2
Query: 110 IGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKERRHMPE 289
+G+NR KNSL KNL+K AV+ L K +R ++I + VP IFCAGADLKER M
Sbjct: 92 LGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAGADLKERAKMSS 151
Query: 290 SDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGLVETG 469
S++ FV R + +A +P+PTI AID CD+R ++ + MGLVET
Sbjct: 152 SEVGPFVSKIRAVINDIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETK 211
Query: 470 LAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSALLHA 649
LAIIPG GGTQRL R IG++ AKELIF+A+ + GKEA+ +G+++ ++QN +G++A A
Sbjct: 212 LAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKA 271
Query: 650 FTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLKAFQE 829
+A+ GP+ +++AK AI+ G+ V V L +EE CYAQ IPT+DRLEGL AF+E
Sbjct: 272 LDLAREFLPQGPVAMRVAKLAINQGMEVDLV-TGLAIEEACYAQTIPTKDRLEGLLAFKE 330
Query: 830 KRKPKYEGK 856
KR P+Y+G+
Sbjct: 331 KRPPRYKGE 339
>sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitochondrial precursor
(AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding enoyl-CoA hydratase) (muAUH)
Length = 314
Score = 223 bits (568), Expect = 6e-58
Identities = 115/249 (46%), Positives = 165/249 (66%)
Frame = +2
Query: 110 IGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKERRHMPE 289
+G+NR KN+L KNL+K AV+ L K +R ++I + VP IFCAGADLKER M
Sbjct: 67 LGINRAYGKNALSKNLLKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAGADLKERAKMHS 126
Query: 290 SDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGLVETG 469
S++ FV R++ +A +P+PTI AID CD+R ++ + MGLVET
Sbjct: 127 SEVGPFVSKIRSVINDIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETK 186
Query: 470 LAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSALLHA 649
LAIIPG GGTQRL R IG++ AKELIF+A+ + G+EA+ +G+++ ++QN +G++A A
Sbjct: 187 LAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGQEAKAVGLISHVLEQNQEGDAAYRKA 246
Query: 650 FTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLKAFQE 829
+A+ GP+ +++AK AI+ G+ V V L +EE CYAQ I T+DRLEGL AF+E
Sbjct: 247 LDLAREFLPQGPVAMRVAKLAINQGMEVDLV-TGLAIEEACYAQTISTKDRLEGLLAFKE 305
Query: 830 KRKPKYEGK 856
KR P+Y+G+
Sbjct: 306 KRPPRYKGE 314
>sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Crotonase)
Length = 261
Score = 142 bits (357), Expect = 2e-33
Identities = 82/247 (33%), Positives = 131/247 (53%)
Frame = +2
Query: 98 KIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKERR 277
K+ + +NRPK+ N+L + +K+ + + + ++ V++ F AGAD+ E +
Sbjct: 13 KVAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSFVAGADISEMK 72
Query: 278 HMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGL 457
M + F +F+ + + P I A++ CD+R +S N G
Sbjct: 73 EMNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDIRIASSNARFGQ 132
Query: 458 VETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSA 637
E GL I PG GGTQRLSRL+G+ AK+LIFTA+ I EA IG+VN V+ + N+A
Sbjct: 133 PEVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNKVVEPSELMNTA 192
Query: 638 LLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLK 817
+A I +N P+ VK++K AI+ G++ + AL E + + + TED+ + +
Sbjct: 193 K----EIANKIVSNAPVAVKLSKQAINRGMQ-CDIDTALAFESEAFGECFSTEDQKDAMT 247
Query: 818 AFQEKRK 838
AF EKRK
Sbjct: 248 AFIEKRK 254
>sp|P64017|ECHA8_MYCBO Probable enoyl-CoA hydratase echA8
sp|P64016|ECHA8_MYCTU Probable enoyl-CoA hydratase echA8
Length = 257
Score = 124 bits (310), Expect = 5e-28
Identities = 80/254 (31%), Positives = 129/254 (50%)
Frame = +2
Query: 86 DHPSKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADL 265
+ ++G I LNRP++ N+L ++ + +A L + DI ++I F AGAD+
Sbjct: 9 ERDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITG-SAKAFAAGADI 67
Query: 266 KERRHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENT 445
KE + +D F + +A + PTI A+ CD+ +++
Sbjct: 68 KEMADLTFADA--FTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTA 125
Query: 446 FMGLVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPD 625
G E L ++PG GG+QRL+R IG AKA +LI T +T+ EAE G+V+ V +
Sbjct: 126 KFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADDL 185
Query: 626 GNSALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRL 805
A A T++QM ++ +MAK A++ SS+ + LL E + + TED+
Sbjct: 186 LTEARATATTISQMSAS----AARMAKEAVNRAFE-SSLSEGLLYERRLFHSAFATEDQS 240
Query: 806 EGLKAFQEKRKPKY 847
EG+ AF EKR P++
Sbjct: 241 EGMAAFIEKRAPQF 254
>sp|O07137|ECHA8_MYCLE Probable enoyl-CoA hydratase echA8
Length = 257
Score = 123 bits (309), Expect = 7e-28
Identities = 82/254 (32%), Positives = 127/254 (50%)
Frame = +2
Query: 86 DHPSKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADL 265
D ++G I LNRP++ N+L ++ + NA L D+ ++I P +F AGAD+
Sbjct: 9 DGYQRVGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITG-SPKVFAAGADI 67
Query: 266 KERRHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENT 445
KE + +D F + + +A + P I A+ CDL +++
Sbjct: 68 KEMASLTFTDA--FDADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADTA 125
Query: 446 FMGLVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPD 625
G E L ++PG GG+QRL+R IG AKA +LI T +TI EAE G+V+ V
Sbjct: 126 KFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRVVL---- 181
Query: 626 GNSALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRL 805
+ L A VA IS +MAK A++ S++ + LL E + + T+D+
Sbjct: 182 ADDLLPEAKAVATTISQMSRSATRMAKEAVNRSFE-STLAEGLLHERRLFHSTFVTDDQS 240
Query: 806 EGLKAFQEKRKPKY 847
EG+ AF EKR P++
Sbjct: 241 EGMAAFIEKRAPQF 254
>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
Length = 288
Score = 120 bits (302), Expect = 4e-27
Identities = 78/253 (30%), Positives = 130/253 (51%), Gaps = 1/253 (0%)
Frame = +2
Query: 101 IGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKERRH 280
+ I LNRPK+ N+L L+ + +A+ +L K + +VI F AGAD+KE
Sbjct: 45 VALIKLNRPKALNALCAQLMTELADALEVLDTDKSVGAIVITG-SERAFAAGADIKE--- 100
Query: 281 MPESDLENFVKGA-RNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGL 457
M ++ G+ + + V+++ P I A++ CD+ Y+ E G
Sbjct: 101 MTNNEFATTFSGSFLSNWTAVSDVKKPVIAAVNGFALGGGNELAMMCDIIYAGEKARFGQ 160
Query: 458 VETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSA 637
E + IPGAGGTQR +R G + A E+ T +T +EA+E GIV+ +
Sbjct: 161 PEINIGTIPGAGGTQRWARAAGKSFAMEVCLTGNHVTAQEAKEHGIVSKIF----PADQV 216
Query: 638 LLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLK 817
+ A + + I++ P+ V+MAK A++ + ++++ L E + + T+DR EG+
Sbjct: 217 VGEAVKLGEKIADQSPLIVQMAKEAVNKAYEL-TLQEGLHFERRLFHATFATKDRKEGMT 275
Query: 818 AFQEKRKPKYEGK 856
AF EKRKP++E K
Sbjct: 276 AFAEKRKPQWESK 288
>sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 117 bits (292), Expect = 6e-26
Identities = 75/252 (29%), Positives = 126/252 (50%)
Frame = +2
Query: 95 SKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKER 274
S +G I LNRPK+ N+L L+++ A+ E + +V+ F AGAD+KE
Sbjct: 45 SSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAFAAGADIKEM 103
Query: 275 RHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 454
++ D + + + + I P I A++ CD+ Y+ E G
Sbjct: 104 QNRTFQDC--YSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFG 161
Query: 455 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 634
E L IPGAGGTQRL+R +G + A E++ T I+ ++A++ G+V+ +
Sbjct: 162 QPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPV----ET 217
Query: 635 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 814
+ A A+ I+NN I V MAK +++ + ++ + +E+K + T+DR EG+
Sbjct: 218 LVEEAIQCAEKIANNSKIIVAMAKESVNAAFEM-TLTEGNKLEKKLFYSTFATDDRREGM 276
Query: 815 KAFQEKRKPKYE 850
AF EKRK ++
Sbjct: 277 SAFVEKRKANFK 288
>sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 117 bits (292), Expect = 6e-26
Identities = 72/252 (28%), Positives = 127/252 (50%)
Frame = +2
Query: 95 SKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKER 274
S +G I LNRPK+ N+L L+ + A+ E + +V+ +F AGAD+KE
Sbjct: 45 SNVGLIQLNRPKALNALCNGLIVELNQALQAFEEDPAVGAIVLTG-GEKVFAAGADIKEM 103
Query: 275 RHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 454
+ + + + G + + + + P I A++ CD+ Y+ E G
Sbjct: 104 QSLTFQNC--YSGGFLSHWDQLTRVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFG 161
Query: 455 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 634
E + IPGAGGTQRL+R +G + A E++ T I+ ++A++ G+V+ +
Sbjct: 162 QPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPV----ET 217
Query: 635 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 814
+ A A+ I++N I MAK +++ + ++ + + +E+K + TEDR EG+
Sbjct: 218 VVEEAIQCAEKIASNSKIVTAMAKESVNAAFEM-TLAEGVKLEKKLFYSTFATEDRKEGM 276
Query: 815 KAFQEKRKPKYE 850
AF EKRK ++
Sbjct: 277 AAFVEKRKANFK 288
>sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF
Length = 255
Score = 114 bits (285), Expect = 4e-25
Identities = 77/255 (30%), Positives = 129/255 (50%), Gaps = 2/255 (0%)
Frame = +2
Query: 98 KIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIR-CVVIGN*VPNIFCAGADLKER 274
++ + LNRP ++N+L L+ N + I CV+ GN F AGADL E
Sbjct: 11 RVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGN--ARFFAAGADLNE- 67
Query: 275 RHMPESDLENFVKGAR-NLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFM 451
M E DL + R L+ + P I A++ CD+ + EN
Sbjct: 68 --MAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARF 125
Query: 452 GLVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGN 631
GL E L I+PGAGGTQRL R +G + A +++ + ++IT ++A++ G+V+ +
Sbjct: 126 GLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVF----PSD 181
Query: 632 SALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEG 811
L +A +A ++ + P+ ++ AK A+ V +++ L E + + + TEDR EG
Sbjct: 182 LTLEYALQLASKMARHSPLALQAAKQALRQSQEV-ALQAGLAQERQLFTLLAATEDRHEG 240
Query: 812 LKAFQEKRKPKYEGK 856
+ AF +KR P ++G+
Sbjct: 241 ISAFLQKRTPDFKGR 255
>sp|P30084|ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 113 bits (283), Expect = 7e-25
Identities = 75/252 (29%), Positives = 126/252 (50%), Gaps = 2/252 (0%)
Frame = +2
Query: 101 IGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGN*VPNIFCAGADLKERRH 280
+G I LNRPK+ N+L L+ + A+ E + +V+ F AGAD+KE ++
Sbjct: 47 VGLIQLNRPKALNALCDGLIDELNQALKTFEEDPAVGAIVLTG-GDKAFAAGADIKEMQN 105
Query: 281 MPESDL--ENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 454
+ D F+K +L Q + P I A++ CD+ Y+ E
Sbjct: 106 LSFQDCYSSKFLKHWDHLTQ----VKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFA 161
Query: 455 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 634
E + IPGAGGTQRL+R +G + A E++ T I+ ++A++ G+V+ +
Sbjct: 162 QPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPV----ET 217
Query: 635 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 814
+ A A+ I++N I V MAK +++ ++ + + L E+K + T+DR EG+
Sbjct: 218 LVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKL-EKKLFYSTFATDDRKEGM 276
Query: 815 KAFQEKRKPKYE 850
AF EKRK ++
Sbjct: 277 TAFVEKRKANFK 288
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105,051,582
Number of Sequences: 369166
Number of extensions: 2197468
Number of successful extensions: 6267
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6003
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6213
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9750928390
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)