Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_P10
(909 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13825|AUMH_HUMAN Methylglutaconyl-CoA hydratase, mitoch... 230 4e-60
sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitoch... 224 2e-58
sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Croto... 143 8e-34
sp|P64017|ECHA8_MYCBO Probable enoyl-CoA hydratase echA8 >g... 124 3e-28
sp|O07137|ECHA8_MYCLE Probable enoyl-CoA hydratase echA8 124 5e-28
sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochon... 121 3e-27
sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precu... 117 6e-26
sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial pre... 117 6e-26
sp|P30084|ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial pre... 113 6e-25
sp|Q5R646|ECHM_PONPY Enoyl-CoA hydratase, mitochondrial pre... 113 8e-25
>sp|Q13825|AUMH_HUMAN Methylglutaconyl-CoA hydratase, mitochondrial precursor
(AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding protein/enoyl-CoA hydratase)
Length = 339
Score = 230 bits (587), Expect = 4e-60
Identities = 119/249 (47%), Positives = 165/249 (66%)
Frame = +3
Query: 90 IGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGSLVPNIFCAGADLKERRHMPE 269
+G+NR KNSL KNL+K AV+ L K +R ++I S VP IFCAGADLKER M
Sbjct: 92 LGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAGADLKERAKMSS 151
Query: 270 SDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGLVETG 449
S++ FV R + +A +P+PTI AID CD+R ++ + MGLVET
Sbjct: 152 SEVGPFVSKIRAVINDIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETK 211
Query: 450 LAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSALLHA 629
LAIIPG GGTQRL R IG++ AKELIF+A+ + GKEA+ +G+++ ++QN +G++A A
Sbjct: 212 LAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKA 271
Query: 630 FTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLKAFQE 809
+A+ GP+ +++AK AI+ G+ V V L +EE CYAQ IPT+DRLEGL AF+E
Sbjct: 272 LDLAREFLPQGPVAMRVAKLAINQGMEVDLV-TGLAIEEACYAQTIPTKDRLEGLLAFKE 330
Query: 810 KRKPKYEGK 836
KR P+Y+G+
Sbjct: 331 KRPPRYKGE 339
>sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitochondrial precursor
(AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding enoyl-CoA hydratase) (muAUH)
Length = 314
Score = 224 bits (572), Expect = 2e-58
Identities = 116/249 (46%), Positives = 165/249 (66%)
Frame = +3
Query: 90 IGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGSLVPNIFCAGADLKERRHMPE 269
+G+NR KN+L KNL+K AV+ L K +R ++I S VP IFCAGADLKER M
Sbjct: 67 LGINRAYGKNALSKNLLKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAGADLKERAKMHS 126
Query: 270 SDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGLVETG 449
S++ FV R++ +A +P+PTI AID CD+R ++ + MGLVET
Sbjct: 127 SEVGPFVSKIRSVINDIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETK 186
Query: 450 LAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSALLHA 629
LAIIPG GGTQRL R IG++ AKELIF+A+ + G+EA+ +G+++ ++QN +G++A A
Sbjct: 187 LAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGQEAKAVGLISHVLEQNQEGDAAYRKA 246
Query: 630 FTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLKAFQE 809
+A+ GP+ +++AK AI+ G+ V V L +EE CYAQ I T+DRLEGL AF+E
Sbjct: 247 LDLAREFLPQGPVAMRVAKLAINQGMEVDLV-TGLAIEEACYAQTISTKDRLEGLLAFKE 305
Query: 810 KRKPKYEGK 836
KR P+Y+G+
Sbjct: 306 KRPPRYKGE 314
>sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Crotonase)
Length = 261
Score = 143 bits (360), Expect = 8e-34
Identities = 82/247 (33%), Positives = 131/247 (53%)
Frame = +3
Query: 78 KIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGSLVPNIFCAGADLKERR 257
K+ + +NRPK+ N+L + +K+ + + + ++ V++ F AGAD+ E +
Sbjct: 13 KVAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSFVAGADISEMK 72
Query: 258 HMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMGL 437
M + F +F+ + + P I A++ CD+R +S N G
Sbjct: 73 EMNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDIRIASSNARFGQ 132
Query: 438 VETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNSA 617
E GL I PG GGTQRLSRL+G+ AK+LIFTA+ I EA IG+VN V+ + N+A
Sbjct: 133 PEVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNKVVEPSELMNTA 192
Query: 618 LLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGLK 797
+A I +N P+ VK++K AI+ G++ + AL E + + + TED+ + +
Sbjct: 193 K----EIANKIVSNAPVAVKLSKQAINRGMQ-CDIDTALAFESEAFGECFSTEDQKDAMT 247
Query: 798 AFQEKRK 818
AF EKRK
Sbjct: 248 AFIEKRK 254
>sp|P64017|ECHA8_MYCBO Probable enoyl-CoA hydratase echA8
sp|P64016|ECHA8_MYCTU Probable enoyl-CoA hydratase echA8
Length = 257
Score = 124 bits (312), Expect = 3e-28
Identities = 83/263 (31%), Positives = 134/263 (50%)
Frame = +3
Query: 39 TEKEIIFDTDHPSKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGSLVP 218
T + I+ + D ++G I LNRP++ N+L ++ + +A L + DI ++I
Sbjct: 2 TYETILVERDQ--RVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSA- 58
Query: 219 NIFCAGADLKERRHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXC 398
F AGAD+KE + +D F + +A + PTI A+ C
Sbjct: 59 KAFAAGADIKEMADLTFADA--FTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMC 116
Query: 399 DLRYSSENTFMGLVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIV 578
D+ +++ G E L ++PG GG+QRL+R IG AKA +LI T +T+ EAE G+V
Sbjct: 117 DVLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLV 176
Query: 579 NACVKQNPDGNSALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYA 758
+ V + A A T++QM ++ +MAK A++ SS+ + LL E + +
Sbjct: 177 SRVVPADDLLTEARATATTISQMSAS----AARMAKEAVNRAFE-SSLSEGLLYERRLFH 231
Query: 759 QIIPTEDRLEGLKAFQEKRKPKY 827
TED+ EG+ AF EKR P++
Sbjct: 232 SAFATEDQSEGMAAFIEKRAPQF 254
>sp|O07137|ECHA8_MYCLE Probable enoyl-CoA hydratase echA8
Length = 257
Score = 124 bits (310), Expect = 5e-28
Identities = 84/255 (32%), Positives = 129/255 (50%), Gaps = 1/255 (0%)
Frame = +3
Query: 66 DHPSKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVI-GSLVPNIFCAGAD 242
D ++G I LNRP++ N+L ++ + NA L D+ ++I GS P +F AGAD
Sbjct: 9 DGYQRVGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITGS--PKVFAAGAD 66
Query: 243 LKERRHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSEN 422
+KE + +D F + + +A + P I A+ CDL +++
Sbjct: 67 IKEMASLTFTDA--FDADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADT 124
Query: 423 TFMGLVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNP 602
G E L ++PG GG+QRL+R IG AKA +LI T +TI EAE G+V+ V
Sbjct: 125 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRVVL--- 181
Query: 603 DGNSALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDR 782
+ L A VA IS +MAK A++ S++ + LL E + + T+D+
Sbjct: 182 -ADDLLPEAKAVATTISQMSRSATRMAKEAVNRSFE-STLAEGLLHERRLFHSTFVTDDQ 239
Query: 783 LEGLKAFQEKRKPKY 827
EG+ AF EKR P++
Sbjct: 240 SEGMAAFIEKRAPQF 254
>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
Length = 288
Score = 121 bits (303), Expect = 3e-27
Identities = 80/254 (31%), Positives = 132/254 (51%), Gaps = 2/254 (0%)
Frame = +3
Query: 81 IGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVI-GSLVPNIFCAGADLKERR 257
+ I LNRPK+ N+L L+ + +A+ +L K + +VI GS F AGAD+KE
Sbjct: 45 VALIKLNRPKALNALCAQLMTELADALEVLDTDKSVGAIVITGS--ERAFAAGADIKE-- 100
Query: 258 HMPESDLENFVKGA-RNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 434
M ++ G+ + + V+++ P I A++ CD+ Y+ E G
Sbjct: 101 -MTNNEFATTFSGSFLSNWTAVSDVKKPVIAAVNGFALGGGNELAMMCDIIYAGEKARFG 159
Query: 435 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 614
E + IPGAGGTQR +R G + A E+ T +T +EA+E GIV+ +
Sbjct: 160 QPEINIGTIPGAGGTQRWARAAGKSFAMEVCLTGNHVTAQEAKEHGIVSKIF----PADQ 215
Query: 615 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 794
+ A + + I++ P+ V+MAK A++ + ++++ L E + + T+DR EG+
Sbjct: 216 VVGEAVKLGEKIADQSPLIVQMAKEAVNKAYEL-TLQEGLHFERRLFHATFATKDRKEGM 274
Query: 795 KAFQEKRKPKYEGK 836
AF EKRKP++E K
Sbjct: 275 TAFAEKRKPQWESK 288
>sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 117 bits (292), Expect = 6e-26
Identities = 75/252 (29%), Positives = 126/252 (50%)
Frame = +3
Query: 75 SKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGSLVPNIFCAGADLKER 254
S +G I LNRPK+ N+L L+++ A+ E + +V+ F AGAD+KE
Sbjct: 45 SSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAFAAGADIKEM 103
Query: 255 RHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 434
++ D + + + + I P I A++ CD+ Y+ E G
Sbjct: 104 QNRTFQDC--YSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFG 161
Query: 435 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 614
E L IPGAGGTQRL+R +G + A E++ T I+ ++A++ G+V+ +
Sbjct: 162 QPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPV----ET 217
Query: 615 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 794
+ A A+ I+NN I V MAK +++ + ++ + +E+K + T+DR EG+
Sbjct: 218 LVEEAIQCAEKIANNSKIIVAMAKESVNAAFEM-TLTEGNKLEKKLFYSTFATDDRREGM 276
Query: 795 KAFQEKRKPKYE 830
AF EKRK ++
Sbjct: 277 SAFVEKRKANFK 288
>sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 117 bits (292), Expect = 6e-26
Identities = 72/252 (28%), Positives = 127/252 (50%)
Frame = +3
Query: 75 SKIGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGSLVPNIFCAGADLKER 254
S +G I LNRPK+ N+L L+ + A+ E + +V+ +F AGAD+KE
Sbjct: 45 SNVGLIQLNRPKALNALCNGLIVELNQALQAFEEDPAVGAIVLTG-GEKVFAAGADIKEM 103
Query: 255 RHMPESDLENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 434
+ + + + G + + + + P I A++ CD+ Y+ E G
Sbjct: 104 QSLTFQNC--YSGGFLSHWDQLTRVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFG 161
Query: 435 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 614
E + IPGAGGTQRL+R +G + A E++ T I+ ++A++ G+V+ +
Sbjct: 162 QPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPV----ET 217
Query: 615 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 794
+ A A+ I++N I MAK +++ + ++ + + +E+K + TEDR EG+
Sbjct: 218 VVEEAIQCAEKIASNSKIVTAMAKESVNAAFEM-TLAEGVKLEKKLFYSTFATEDRKEGM 276
Query: 795 KAFQEKRKPKYE 830
AF EKRK ++
Sbjct: 277 AAFVEKRKANFK 288
>sp|P30084|ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 113 bits (283), Expect = 6e-25
Identities = 75/252 (29%), Positives = 126/252 (50%), Gaps = 2/252 (0%)
Frame = +3
Query: 81 IGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGSLVPNIFCAGADLKERRH 260
+G I LNRPK+ N+L L+ + A+ E + +V+ F AGAD+KE ++
Sbjct: 47 VGLIQLNRPKALNALCDGLIDELNQALKTFEEDPAVGAIVLTG-GDKAFAAGADIKEMQN 105
Query: 261 MPESDL--ENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 434
+ D F+K +L Q + P I A++ CD+ Y+ E
Sbjct: 106 LSFQDCYSSKFLKHWDHLTQ----VKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFA 161
Query: 435 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 614
E + IPGAGGTQRL+R +G + A E++ T I+ ++A++ G+V+ +
Sbjct: 162 QPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPV----ET 217
Query: 615 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 794
+ A A+ I++N I V MAK +++ ++ + + L E+K + T+DR EG+
Sbjct: 218 LVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKL-EKKLFYSTFATDDRKEGM 276
Query: 795 KAFQEKRKPKYE 830
AF EKRK ++
Sbjct: 277 TAFVEKRKANFK 288
>sp|Q5R646|ECHM_PONPY Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 113 bits (282), Expect = 8e-25
Identities = 76/252 (30%), Positives = 125/252 (49%), Gaps = 2/252 (0%)
Frame = +3
Query: 81 IGFIGLNRPKSKNSLGKNLVKDFENAVNILHETKDIRCVVIGSLVPNIFCAGADLKERRH 260
+G I LNRPK+ N+L L+ + A+ I E + +V+ F AGAD+KE ++
Sbjct: 47 VGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTG-GDKAFAAGADIKEMQN 105
Query: 261 MPESDL--ENFVKGARNLFQLVAEIPIPTICAIDXXXXXXXXXXXXXCDLRYSSENTFMG 434
+ D F+K +L Q I P I A++ CD+ Y+ E
Sbjct: 106 LSFQDCYSSKFLKHWDHLTQ----IKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFA 161
Query: 435 LVETGLAIIPGAGGTQRLSRLIGLAKAKELIFTAKTITGKEAEEIGIVNACVKQNPDGNS 614
E + IPGAGGTQRL+R +G + A E++ T I+ ++A++ G+V+ +
Sbjct: 162 QPEILIGTIPGAGGTQRLTRTVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPV----ET 217
Query: 615 ALLHAFTVAQMISNNGPIGVKMAKAAIDGGIRVSSVKDALLVEEKCYAQIIPTEDRLEGL 794
+ A A+ I++N I MAK +++ ++ + + L E+K + T DR EG+
Sbjct: 218 LVEEAIQCAEKIASNSKIIAAMAKESVNAAFEMTLAEGSKL-EKKLFYSTFATNDRKEGM 276
Query: 795 KAFQEKRKPKYE 830
AF EKRK ++
Sbjct: 277 TAFVEKRKANFK 288
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 102,839,685
Number of Sequences: 369166
Number of extensions: 2151853
Number of successful extensions: 6325
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6041
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6270
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9222552960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)