Planaria EST Database

DrC_00621

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00621
         (704 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9Z255|UBE2A_MOUSE  Ubiquitin-conjugating enzyme E2 A (Ub...   250   2e-66
sp|P52478|UBC1_CAEEL  Ubiquitin-conjugating enzyme E2 1 (Ubi...   249   5e-66
sp|P63146|UBE2B_HUMAN  Ubiquitin-conjugating enzyme E2 B (Ub...   249   7e-66
sp|P25153|UBCD6_DROME  Ubiquitin-conjugating enzyme E2-17 kD...   247   2e-65
sp|P35130|UBC2_MEDSA  Ubiquitin-conjugating enzyme E2-17 kDa...   231   1e-60
sp|P25865|UBC1_ARATH  Ubiquitin-conjugating enzyme E2-17 kDa...   230   2e-60
sp|P42745|UBC2_ARATH  Ubiquitin-conjugating enzyme E2-17 kDa...   230   3e-60
sp|P25866|UBC2_WHEAT  Ubiquitin-conjugating enzyme E2-17 kDa...   229   7e-60
sp|P23566|UBC2_SCHPO  Ubiquitin-conjugating enzyme E2-17 kDa...   217   2e-56
sp|O74201|UBC2_CANAL  Ubiquitin-conjugating enzyme E2-20 kDa...   214   2e-55

>sp|Q9Z255|UBE2A_MOUSE Ubiquitin-conjugating enzyme E2 A (Ubiquitin-protein ligase A)
           (Ubiquitin carrier protein A) (HR6A) (mHR6A)
 sp|P49459|UBE2A_HUMAN Ubiquitin-conjugating enzyme E2 A (Ubiquitin-protein ligase A)
           (Ubiquitin carrier protein A) (HR6A) (hHR6A)
          Length = 152

 Score =  250 bits (639), Expect = 2e-66
 Identities = 118/149 (79%), Positives = 130/149 (87%)
 Frame = +2

Query: 35  MGSAAQRRLIRDLKRLQDDPPAGVCGAPIDDNIMYWNAVIFGPDGTPFEDGTFNLRLEFS 214
           M + A+RRL+RD KRLQ+DPPAGV GAP ++NIM WNAVIFGP+GTPFEDGTF L +EF+
Sbjct: 1   MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFT 60

Query: 215 EEYPNKPPRVVFTSKMFHPNVYADGSICLDILGNTWSPTYDVIAILTSIQSLLDEPNPNS 394
           EEYPNKPP V F SKMFHPNVYADGSICLDIL N WSPTYDV +ILTSIQSLLDEPNPNS
Sbjct: 61  EEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNS 120

Query: 395 PANSVAAQLYTENRREYEKRVKECVEESW 481
           PANS AAQLY EN+REYEKRV   VE+SW
Sbjct: 121 PANSQAAQLYQENKREYEKRVSAIVEQSW 149

>sp|P52478|UBC1_CAEEL Ubiquitin-conjugating enzyme E2 1 (Ubiquitin-protein ligase 1)
           (Ubiquitin carrier protein 1)
          Length = 192

 Score =  249 bits (636), Expect = 5e-66
 Identities = 117/150 (78%), Positives = 131/150 (87%)
 Frame = +2

Query: 35  MGSAAQRRLIRDLKRLQDDPPAGVCGAPIDDNIMYWNAVIFGPDGTPFEDGTFNLRLEFS 214
           M + ++RRL+RD K+LQ+DPPAGV GAP +DNI+ W A+IFGP  TPFEDGTF L LEF+
Sbjct: 1   MTTPSRRRLMRDFKKLQEDPPAGVSGAPTEDNILTWEAIIFGPQETPFEDGTFKLSLEFT 60

Query: 215 EEYPNKPPRVVFTSKMFHPNVYADGSICLDILGNTWSPTYDVIAILTSIQSLLDEPNPNS 394
           EEYPNKPP V F SKMFHPNVYADGSICLDIL N WSPTYDV AILTSIQSLLDEPNPNS
Sbjct: 61  EEYPNKPPTVKFISKMFHPNVYADGSICLDILQNRWSPTYDVAAILTSIQSLLDEPNPNS 120

Query: 395 PANSVAAQLYTENRREYEKRVKECVEESWL 484
           PANS+AAQLY ENRREYEKRV++ VE+SWL
Sbjct: 121 PANSLAAQLYQENRREYEKRVQQIVEQSWL 150

>sp|P63146|UBE2B_HUMAN Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
           (Ubiquitin carrier protein B) (HR6B) (hHR6B) (E2-17 kDa)
 sp|P63148|UBE2B_RABIT Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
           (Ubiquitin carrier protein B) (HR6B) (E2(14k))
 sp|P63147|UBE2B_MOUSE Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
           (Ubiquitin carrier protein B) (HR6B) (E214K)
 sp|P63149|UBE2B_RAT Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
           (Ubiquitin carrier protein B) (HR6B) (E2(14k))
          Length = 152

 Score =  249 bits (635), Expect = 7e-66
 Identities = 118/149 (79%), Positives = 129/149 (86%)
 Frame = +2

Query: 35  MGSAAQRRLIRDLKRLQDDPPAGVCGAPIDDNIMYWNAVIFGPDGTPFEDGTFNLRLEFS 214
           M + A+RRL+RD KRLQ+DPP GV GAP ++NIM WNAVIFGP+GTPFEDGTF L +EFS
Sbjct: 1   MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFS 60

Query: 215 EEYPNKPPRVVFTSKMFHPNVYADGSICLDILGNTWSPTYDVIAILTSIQSLLDEPNPNS 394
           EEYPNKPP V F SKMFHPNVYADGSICLDIL N WSPTYDV +ILTSIQSLLDEPNPNS
Sbjct: 61  EEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNS 120

Query: 395 PANSVAAQLYTENRREYEKRVKECVEESW 481
           PANS AAQLY EN+REYEKRV   VE+SW
Sbjct: 121 PANSQAAQLYQENKREYEKRVSAIVEQSW 149

>sp|P25153|UBCD6_DROME Ubiquitin-conjugating enzyme E2-17 kDa (Ubiquitin-protein ligase)
           (Ubiquitin carrier protein)
          Length = 151

 Score =  247 bits (630), Expect = 2e-65
 Identities = 116/151 (76%), Positives = 129/151 (85%)
 Frame = +2

Query: 35  MGSAAQRRLIRDLKRLQDDPPAGVCGAPIDDNIMYWNAVIFGPDGTPFEDGTFNLRLEFS 214
           M + A+RRL+RD KRLQ+DPP GV GAP D+NIM WNAVIFGP  TPFEDGTF L +EF+
Sbjct: 1   MSTPARRRLMRDFKRLQEDPPTGVSGAPTDNNIMIWNAVIFGPHDTPFEDGTFKLTIEFT 60

Query: 215 EEYPNKPPRVVFTSKMFHPNVYADGSICLDILGNTWSPTYDVIAILTSIQSLLDEPNPNS 394
           EEYPNKPP V F SK+FHPNVYADG ICLDIL N WSPTYDV AILTSIQSLL +PNPNS
Sbjct: 61  EEYPNKPPTVRFVSKVFHPNVYADGGICLDILQNRWSPTYDVSAILTSIQSLLSDPNPNS 120

Query: 395 PANSVAAQLYTENRREYEKRVKECVEESWLE 487
           PANS AAQLY ENRREYEKRVK CVE+S+++
Sbjct: 121 PANSTAAQLYKENRREYEKRVKACVEQSFID 151

>sp|P35130|UBC2_MEDSA Ubiquitin-conjugating enzyme E2-17 kDa (Ubiquitin-protein ligase)
           (Ubiquitin carrier protein)
          Length = 152

 Score =  231 bits (590), Expect = 1e-60
 Identities = 106/149 (71%), Positives = 127/149 (85%)
 Frame = +2

Query: 35  MGSAAQRRLIRDLKRLQDDPPAGVCGAPIDDNIMYWNAVIFGPDGTPFEDGTFNLRLEFS 214
           M + A++RL+RD KRLQ DPPAG+ GAP D+NIM WNAVIFGPD TP++ GTF L L+FS
Sbjct: 1   MSTPARKRLMRDFKRLQHDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFS 60

Query: 215 EEYPNKPPRVVFTSKMFHPNVYADGSICLDILGNTWSPTYDVIAILTSIQSLLDEPNPNS 394
           E+YPNKPP V F S+MFHPN+YADGSICLDIL N WSP YDV AILTSIQSLL +PNPNS
Sbjct: 61  EDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNS 120

Query: 395 PANSVAAQLYTENRREYEKRVKECVEESW 481
           PANS AA++++EN+REY +RV+E VE+SW
Sbjct: 121 PANSEAARMFSENKREYNRRVREVVEQSW 149

>sp|P25865|UBC1_ARATH Ubiquitin-conjugating enzyme E2-17 kDa 1 (Ubiquitin-protein ligase
           1) (Ubiquitin carrier protein 1)
          Length = 152

 Score =  230 bits (587), Expect = 2e-60
 Identities = 105/149 (70%), Positives = 127/149 (85%)
 Frame = +2

Query: 35  MGSAAQRRLIRDLKRLQDDPPAGVCGAPIDDNIMYWNAVIFGPDGTPFEDGTFNLRLEFS 214
           M + A++RL+RD KRLQ DPPAG+ GAP D+NIM WNAVIFGPD TP++ GTF L L+FS
Sbjct: 1   MSTPARKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFS 60

Query: 215 EEYPNKPPRVVFTSKMFHPNVYADGSICLDILGNTWSPTYDVIAILTSIQSLLDEPNPNS 394
           E+YPNKPP V F S+MFHPN+YADGSICLDIL N WSP YDV AILTSIQSLL +PNPNS
Sbjct: 61  EDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNS 120

Query: 395 PANSVAAQLYTENRREYEKRVKECVEESW 481
           PANS AA++Y+E++REY +RV++ VE+SW
Sbjct: 121 PANSEAARMYSESKREYNRRVRDVVEQSW 149

>sp|P42745|UBC2_ARATH Ubiquitin-conjugating enzyme E2-17 kDa 2 (Ubiquitin-protein ligase
           2) (Ubiquitin carrier protein 2)
          Length = 152

 Score =  230 bits (586), Expect = 3e-60
 Identities = 105/149 (70%), Positives = 127/149 (85%)
 Frame = +2

Query: 35  MGSAAQRRLIRDLKRLQDDPPAGVCGAPIDDNIMYWNAVIFGPDGTPFEDGTFNLRLEFS 214
           M + A++RL+RD KRLQ DPPAG+ GAP D+NIM WNAVIFGPD TP++ GTF L L+FS
Sbjct: 1   MSTPARKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFS 60

Query: 215 EEYPNKPPRVVFTSKMFHPNVYADGSICLDILGNTWSPTYDVIAILTSIQSLLDEPNPNS 394
           E+YPNKPP V F S+MFHPN+YADGSICLDIL N WSP YDV AILTSIQSLL +PNPNS
Sbjct: 61  EDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNS 120

Query: 395 PANSVAAQLYTENRREYEKRVKECVEESW 481
           PANS AA++++E++REY +RV+E VE+SW
Sbjct: 121 PANSEAARMFSESKREYNRRVREVVEQSW 149

>sp|P25866|UBC2_WHEAT Ubiquitin-conjugating enzyme E2-17 kDa (Ubiquitin-protein ligase)
           (Ubiquitin carrier protein)
          Length = 152

 Score =  229 bits (583), Expect = 7e-60
 Identities = 104/149 (69%), Positives = 126/149 (84%)
 Frame = +2

Query: 35  MGSAAQRRLIRDLKRLQDDPPAGVCGAPIDDNIMYWNAVIFGPDGTPFEDGTFNLRLEFS 214
           M + A++RL+RD KRLQ DPPAG+ GAP D+NI  WNAVIFGPD TP++ GTF L L+F+
Sbjct: 1   MSTPARKRLMRDFKRLQQDPPAGISGAPHDNNITLWNAVIFGPDDTPWDGGTFKLTLQFT 60

Query: 215 EEYPNKPPRVVFTSKMFHPNVYADGSICLDILGNTWSPTYDVIAILTSIQSLLDEPNPNS 394
           E+YPNKPP V F S+MFHPN+YADGSICLDIL N WSP YDV AILTSIQSLL +PNPNS
Sbjct: 61  EDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNS 120

Query: 395 PANSVAAQLYTENRREYEKRVKECVEESW 481
           PANS AA++Y+EN+REY ++V+E VE+SW
Sbjct: 121 PANSEAARMYSENKREYNRKVREVVEQSW 149

>sp|P23566|UBC2_SCHPO Ubiquitin-conjugating enzyme E2-17 kDa (Ubiquitin-protein ligase 2)
           (Ubiquitin carrier protein) (RAD6 homolog)
          Length = 151

 Score =  217 bits (553), Expect = 2e-56
 Identities = 100/149 (67%), Positives = 120/149 (80%)
 Frame = +2

Query: 35  MGSAAQRRLIRDLKRLQDDPPAGVCGAPIDDNIMYWNAVIFGPDGTPFEDGTFNLRLEFS 214
           M + A+RRL+RD KR+Q DPPAGV  +P+ DN+M WNAVI GP  TPFEDGTF L L F 
Sbjct: 1   MSTTARRRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFD 60

Query: 215 EEYPNKPPRVVFTSKMFHPNVYADGSICLDILGNTWSPTYDVIAILTSIQSLLDEPNPNS 394
           E+YPNKPP V F S MFHPNVYA+G +CLDIL N WSPTYDV AILTSIQSLL++PN  S
Sbjct: 61  EQYPNKPPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNNAS 120

Query: 395 PANSVAAQLYTENRREYEKRVKECVEESW 481
           PAN+ AAQL+ EN++EY +RV++ VE+SW
Sbjct: 121 PANAEAAQLHRENKKEYVRRVRKTVEDSW 149

>sp|O74201|UBC2_CANAL Ubiquitin-conjugating enzyme E2-20 kDa (Ubiquitin-protein ligase)
           (Ubiquitin carrier protein)
          Length = 179

 Score =  214 bits (545), Expect = 2e-55
 Identities = 99/154 (64%), Positives = 120/154 (77%)
 Frame = +2

Query: 35  MGSAAQRRLIRDLKRLQDDPPAGVCGAPIDDNIMYWNAVIFGPDGTPFEDGTFNLRLEFS 214
           M + A+RRL+RD KR+Q DPP+GV  +P+ DN+M WNAVI GP  TPFEDGTF L L F 
Sbjct: 1   MSTPARRRLMRDFKRMQQDPPSGVSASPLPDNVMKWNAVIIGPSDTPFEDGTFRLLLSFD 60

Query: 215 EEYPNKPPRVVFTSKMFHPNVYADGSICLDILGNTWSPTYDVIAILTSIQSLLDEPNPNS 394
           E+YPNKPP+V F S+MFHPNVYA G +CLDIL N WSPTYDV +ILTS+QSLL++PN +S
Sbjct: 61  EQYPNKPPQVKFISEMFHPNVYASGELCLDILQNRWSPTYDVSSILTSVQSLLNDPNISS 120

Query: 395 PANSVAAQLYTENRREYEKRVKECVEESWLEKDD 496
           PAN  AA LY ++R  Y KRV+E VE SW + DD
Sbjct: 121 PANVEAANLYKDHRSLYVKRVRETVENSWNDDDD 154

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,220,814
Number of Sequences: 369166
Number of extensions: 1836206
Number of successful extensions: 4979
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4745
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4916
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6170718545
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00621

  1. Dr_sW_009_C01
  2. Dr_sW_016_G05
  3. Dr_sW_023_N17