Planaria EST Database

DrC_00588

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00588
         (1120 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q58CY8|TSN18_BOVIN  Tetraspanin-18 (Tspan-18)                  129   1e-29
sp|Q96SJ8|TSN18_HUMAN  Tetraspanin-18 (Tspan-18)                  126   1e-28
sp|Q80WR1|TSN18_MOUSE  Tetraspanin-18 (Tspan-18)                  124   4e-28
sp|O75954|TSN9_HUMAN  Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)   102   2e-21
sp|Q8BJU2|TSN9_MOUSE  Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)   100   6e-21
sp|P19075|TSN8_HUMAN  Tetraspanin-8 (Tspan-8) (Transmembrane...    98   4e-20
sp|P24485|CD53_RAT  Leukocyte surface antigen CD53 (Cell sur...    97   6e-20
sp|P27701|CD82_HUMAN  CD82 antigen (Inducible membrane prote...    97   1e-19
sp|O70352|CD82_RAT  CD82 antigen (Metastasis suppressor homo...    96   1e-19
sp|P40237|CD82_MOUSE  CD82 antigen (Inducible membrane prote...    96   2e-19

>sp|Q58CY8|TSN18_BOVIN Tetraspanin-18 (Tspan-18)
          Length = 249

 Score =  129 bits (325), Expect = 1e-29
 Identities = 84/250 (33%), Positives = 134/250 (53%), Gaps = 10/250 (4%)
 Frame = +3

Query: 63  SCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIG-YIVLGCGGTII 239
           SC+K+L+  FNF   + G  + G+GIW+ V P+   E++  N   I G YI+L  GG + 
Sbjct: 7   SCMKYLMFVFNFFIFLGGACLLGIGIWVMVDPTGFREIVAANPLLITGAYILLAMGGLLF 66

Query: 240 LISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLTEKLKT 419
           L+ F GC GA  EN+CLL  FF  +  IF+A L A+IL  IFR     LT++  T++L  
Sbjct: 67  LLGFLGCCGAVRENKCLLLFFFLFILIIFLAELSAAILAFIFR---GNLTREFFTKELTK 123

Query: 420 QY-GSN--SAWTEFWDIVQTKFSCCGVEFSKSHA-ISVFQKTRWYENSKPYKLPDSCCKM 587
            Y GSN    ++  W+ V   F CCGV   +     SVF+      +    ++P++CC+ 
Sbjct: 124 HYQGSNDTDVFSATWNSVMITFGCCGVNGPEDFKYASVFRLLTLDSD----EVPEACCRR 179

Query: 588 KGGQIENVGICRQ----GNENGIHENGCMTVVMKSIGEQYIIVLSAL-ITLAVVLIFGMC 752
           +    + V + R+    G +  +++ GC TV++ +  E Y+ +  AL I +  + +F M 
Sbjct: 180 EPQSRDGVLLSREECLLGRDLFLNKQGCYTVILNAF-ETYVYLAGALAIGVLAIELFAMI 238

Query: 753 LSLAHLRNII 782
            ++   R II
Sbjct: 239 FAMCLFRGII 248

>sp|Q96SJ8|TSN18_HUMAN Tetraspanin-18 (Tspan-18)
          Length = 248

 Score =  126 bits (316), Expect = 1e-28
 Identities = 79/244 (32%), Positives = 129/244 (52%), Gaps = 13/244 (5%)
 Frame = +3

Query: 63  SCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIG-YIVLGCGGTII 239
           SC+K+L+  FNF   + G  +  +GIW+ V P+   E++  N   + G YI+L  GG + 
Sbjct: 7   SCMKYLMFVFNFFIFLGGACLLAIGIWVMVDPTGFREIVAANPLLLTGAYILLAMGGLLF 66

Query: 240 LISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLTEKLKT 419
           L+ F GC GA  EN+CLL  FF  +  IF+A L A+IL  IFR++L   T++  T++L  
Sbjct: 67  LLGFLGCCGAVRENKCLLLFFFLFILIIFLAELSAAILAFIFRENL---TREFFTKELTK 123

Query: 420 QYGSNSAWTEF---WDIVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDSCCKMK 590
            Y  N+    F   W+ V   F CCGV   +    +   +    ++ +   +P++CC+ +
Sbjct: 124 HYQGNNDTDVFSATWNSVMITFGCCGVNGPEDFKFASVFRLLTLDSEE---VPEACCRRE 180

Query: 591 GGQIENVGICRQ----GNENGIHENGCMTVVMKSIGEQYIIVLSALI--TLAV---VLIF 743
               + V + R+    G    +++ GC TV++ +  E Y+ +  AL    LA+    +IF
Sbjct: 181 PQSRDGVLLSREECLLGRSLFLNKQGCYTVILNTF-ETYVYLAGALAIGVLAIELFAMIF 239

Query: 744 GMCL 755
            MCL
Sbjct: 240 AMCL 243

>sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18)
          Length = 248

 Score =  124 bits (312), Expect = 4e-28
 Identities = 80/244 (32%), Positives = 127/244 (52%), Gaps = 13/244 (5%)
 Frame = +3

Query: 63  SCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIG-YIVLGCGGTII 239
           SC+K+L+  FNF   + G  + G+GIW+ V P+   E++  N     G YIVL  GG + 
Sbjct: 7   SCMKYLMFVFNFFVFLGGACLLGVGIWVLVDPTGFREIVATNPLLTTGAYIVLAMGGLLF 66

Query: 240 LISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLTEKLKT 419
           L+ F GC GA  ENRCLL  FF  +  IF+  L A+IL  IFR+ L   T++  T++L  
Sbjct: 67  LLGFLGCCGAVRENRCLLLFFFLFILIIFLVELSAAILAFIFREHL---TREFFTKELTK 123

Query: 420 QY-GSNSA--WTEFWDIVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDSCCKMK 590
            Y G N    ++  W+ V   F CCGV   +   ++   +    +  +   +P +CC+ +
Sbjct: 124 HYQGDNDTDVFSATWNSVMITFGCCGVNGPEDFKLASVFRLLTLDTEE---VPKACCRRE 180

Query: 591 GGQIENVGI----CRQGNENGIHENGCMTVVMKSIGEQYIIVLSALI--TLAV---VLIF 743
               + V +    C+ G    I++ GC TV++ +  E Y+ +  A     LA+   +++F
Sbjct: 181 PQTRDGVVLSREECQLGRNPFINKQGCYTVILNTF-ETYVYLAGAFAIGVLAIELFLMVF 239

Query: 744 GMCL 755
            MCL
Sbjct: 240 AMCL 243

>sp|O75954|TSN9_HUMAN Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)
          Length = 239

 Score =  102 bits (255), Expect = 2e-21
 Identities = 65/246 (26%), Positives = 110/246 (44%), Gaps = 1/246 (0%)
 Frame = +3

Query: 45  MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIGYIVLGC 224
           M +    C+K+++  FN +F +CG  + G+GIW+ VS            +     +V+  
Sbjct: 1   MARGCLCCLKYMMFLFNLIFWLCGCGLLGVGIWLSVSQGNFATFSPSFPSLSAANLVIAI 60

Query: 225 GGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLT 404
           G  +++  F GC GA  EN+CLL  FF VL  I +A L+  IL  ++   +++  K  L 
Sbjct: 61  GTIVMVTGFLGCLGAIKENKCLLLSFFIVLLVILLAELILLILFFVYMDKVNENAKKDLK 120

Query: 405 EKLKTQYGSNSAWTE-FWDIVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDSCC 581
           E L   +  N+   +  W+I+Q +  CCGV             T WY       +PD CC
Sbjct: 121 EGLLLYHTENNVGLKNAWNIIQAEMRCCGVT----------DYTDWYPVLGENTVPDRCC 170

Query: 582 KMKGGQIENVGICRQGNENGIHENGCMTVVMKSIGEQYIIVLSALITLAVVLIFGMCLSL 761
                 +EN   C +     +   GC   V     +   ++ +  + + ++ I GM  S+
Sbjct: 171 ------MENSQGCGRNATTPLWRTGCYEKVKMWFDDNKHVLGTVGMCILIMQILGMAFSM 224

Query: 762 AHLRNI 779
              ++I
Sbjct: 225 TLFQHI 230

>sp|Q8BJU2|TSN9_MOUSE Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)
          Length = 239

 Score =  100 bits (250), Expect = 6e-21
 Identities = 65/246 (26%), Positives = 109/246 (44%), Gaps = 1/246 (0%)
 Frame = +3

Query: 45  MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIGYIVLGC 224
           M +    C+K+ +  FN +F +CG  + G+GIW+ VS            +     +V+  
Sbjct: 1   MARGCLCCLKYTMFLFNLIFWLCGCGLLGVGIWLSVSQGNFATFSPSFPSLSAANLVIAI 60

Query: 225 GGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLT 404
           G  +++  F GC GA  EN+CLL  FF VL  I +A L+  IL  ++   +++  K  L 
Sbjct: 61  GTIVMVTGFLGCLGAIKENKCLLLSFFIVLLIILLAELILIILFFVYMDKVNENAKQDLK 120

Query: 405 EKLKTQYGSNSAWTE-FWDIVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDSCC 581
           E L      N+   +  W+I+Q +  CCGV             T WY       +PD CC
Sbjct: 121 EGLLLYNTENNVGLKNAWNIIQAEMRCCGVT----------DYTDWYPVLGENTVPDRCC 170

Query: 582 KMKGGQIENVGICRQGNENGIHENGCMTVVMKSIGEQYIIVLSALITLAVVLIFGMCLSL 761
                 +EN   C + +   +   GC   V     +   ++ +  + + ++ I GM  S+
Sbjct: 171 ------MENSQGCGRNSTTPLWRTGCYEKVKLWFDDNKHVLGTVGMCILIMQILGMAFSM 224

Query: 762 AHLRNI 779
              ++I
Sbjct: 225 TLFQHI 230

>sp|P19075|TSN8_HUMAN Tetraspanin-8 (Tspan-8) (Transmembrane 4 superfamily member 3)
           (Tumor-associated antigen CO-029)
          Length = 237

 Score = 98.2 bits (243), Expect = 4e-20
 Identities = 65/240 (27%), Positives = 113/240 (47%), Gaps = 7/240 (2%)
 Frame = +3

Query: 63  SCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIGY----IVLGCGG 230
           +C+K+ +  FNFLF +CG+ I  L IW+ VS + S  + G        Y    I++  G 
Sbjct: 6   ACIKYSMFTFNFLFWLCGILILALAIWVRVS-NDSQAIFGSEDVGSSSYVAVDILIAVGA 64

Query: 231 TIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLTEK 410
            I+++ F GC GA  E+RC+L LFF  L  I +  +   IL ++F+    ++  + L E 
Sbjct: 65  IIMILGFLGCCGAIKESRCMLLLFFIGLLLILLLQVATGILGAVFKSKSDRIVNETLYEN 124

Query: 411 LKTQYGSNSAWTEFWD---IVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDSCC 581
            K    +  +  +F +   + Q +F CCG+         V     W  N + Y  P+ C 
Sbjct: 125 TKLLSATGESEKQFQEAIIVFQEEFKCCGL---------VNGAADWGNNFQHY--PELC- 172

Query: 582 KMKGGQIENVGICRQGNENGIHENGCMTVVMKSIGEQYIIVLSALITLAVVLIFGMCLSL 761
                 ++    C+  N   +++  C++ +   + +  IIV+     LAV+ I G+  S+
Sbjct: 173 ----ACLDKQRPCQSYNGKQVYKETCISFIKDFLAKNLIIVIGISFGLAVIEILGLVFSM 228

>sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
           (Leukocyte antigen MRC OX-44)
          Length = 219

 Score = 97.4 bits (241), Expect = 6e-20
 Identities = 51/153 (33%), Positives = 91/153 (59%), Gaps = 1/153 (0%)
 Frame = +3

Query: 45  MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPI-IGYIVLG 221
           MG  +   +K++L FFNFLF +CG  I G GI + V  +    ++ +NL  + +G +++ 
Sbjct: 1   MGMSSLKLLKYVLFFFNFLFWVCGCCILGFGIHLLVQNTYG--ILFRNLPFLTLGNVLVI 58

Query: 222 CGGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQL 401
            G  I++++F GC G+  EN+CLL  FF +L  I +A +  +IL+ ++ K ++ L  + L
Sbjct: 59  VGSIIMVVAFLGCMGSIKENKCLLMSFFVLLLLILLAEVTLAILLFVYEKKINTLVAEGL 118

Query: 402 TEKLKTQYGSNSAWTEFWDIVQTKFSCCGVEFS 500
            + ++  Y S+++    WD +Q++  CCGV  S
Sbjct: 119 NDSIQ-HYHSDNSTRMAWDFIQSQLQCCGVNGS 150

>sp|P27701|CD82_HUMAN CD82 antigen (Inducible membrane protein R2) (C33 antigen) (IA4)
           (Metastasis suppressor Kangai 1) (Suppressor of
           tumorigenicity-6) (Tetraspanin-27) (Tspan-27)
          Length = 267

 Score = 96.7 bits (239), Expect = 1e-19
 Identities = 73/277 (26%), Positives = 129/277 (46%), Gaps = 25/277 (9%)
 Frame = +3

Query: 45  MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPII--GYIVL 218
           MG       K+ L  FN +F I G  I G G+W+    S+   ++  + + +    Y+ +
Sbjct: 1   MGSACIKVTKYFLFLFNLIFFILGAVILGFGVWILADKSSFISVLQTSSSSLRMGAYVFI 60

Query: 219 GCGGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQ 398
           G G   +L+ F GC GA  E RCLL L+F  L  I +A + A    ++F  ++ KL ++ 
Sbjct: 61  GVGAVTMLMGFLGCIGAVNEVRCLLGLYFAFLLLILIAQVTAG---ALFYFNMGKLKQEM 117

Query: 399 --LTEKLKTQYGSN--SAWTEFWDIVQTKFSCCG-VEFSKSHAISVFQKTRWYENSKPYK 563
             +  +L   Y S+   +  + WD VQ +  CCG V F             W +N++   
Sbjct: 118 GGIVTELIRDYNSSREDSLQDAWDYVQAQVKCCGWVSF-----------YNWTDNAELMN 166

Query: 564 LPD----SCCKMKGGQIENV----GIC-------RQGN---ENGIHENGCMTVVMKSIGE 689
            P+      C++KG +  ++    G C       + GN   +  +++ GCM  V   + E
Sbjct: 167 RPEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWPVYQEGCMEKVQAWLQE 226

Query: 690 QYIIVLSALITLAVVLIFGMCLSLAHLRNIINIRYNE 800
              I+L   + +A++ + GM LS+   R++ +  Y++
Sbjct: 227 NLGIILGVGVGVAIIELLGMVLSICLCRHVHSEDYSK 263

>sp|O70352|CD82_RAT CD82 antigen (Metastasis suppressor homolog)
          Length = 266

 Score = 96.3 bits (238), Expect = 1e-19
 Identities = 73/267 (27%), Positives = 117/267 (43%), Gaps = 15/267 (5%)
 Frame = +3

Query: 45  MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPI-IG-YIVL 218
           MG       K+ L  FN LF I G  I G G+W+    S+   ++  + + + +G Y+ +
Sbjct: 1   MGAGCVKVTKYFLFLFNLLFFILGAVILGFGVWILADKSSFISVLQTSSSSLQVGAYVFI 60

Query: 219 GCGGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQ 398
           G G   +L+ F GC GA  E RCLL L+F  L  I +A +   +L       L +   + 
Sbjct: 61  GVGAITMLMGFLGCIGAVNEVRCLLGLYFVFLLLILIAQVTVEVLFYFNANKLKQEMGNT 120

Query: 399 LTEKLKT-QYGSNSAWTEFWDIVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDS 575
           + + ++     ++S+  E WD VQ +  CCG       + S + +    +NS     P S
Sbjct: 121 VMDIIQNYSVNASSSREEAWDYVQAQVKCCGWV-----SPSNWTRNPVLKNSTKTTYPCS 175

Query: 576 CCKMK---GGQIENVGICRQGNENG---------IHENGCMTVVMKSIGEQYIIVLSALI 719
           C K K      I   G C   N            +H  GCM      + E + I+L    
Sbjct: 176 CEKTKEEDNQLIVKKGFCESDNSTASENSPEDWPVHPEGCMEKAQAWLQENFGILLGVCA 235

Query: 720 TLAVVLIFGMCLSLAHLRNIINIRYNE 800
            +AV+ + G+ LS+   R I +  Y++
Sbjct: 236 GVAVIELLGLFLSICLCRYIHSEDYSK 262

>sp|P40237|CD82_MOUSE CD82 antigen (Inducible membrane protein R2) (C33 antigen) (IA4)
          Length = 266

 Score = 95.9 bits (237), Expect = 2e-19
 Identities = 72/263 (27%), Positives = 122/263 (46%), Gaps = 11/263 (4%)
 Frame = +3

Query: 45  MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPI-IG-YIVL 218
           MG       K+ L  FN LF I G  I G G+W+    ++   ++  + + + +G Y+ +
Sbjct: 1   MGAGCVKVTKYFLFLFNLLFFILGAVILGFGVWILADKNSFISVLQTSSSSLQVGAYVFI 60

Query: 219 GCGGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQ 398
           G G   I++ F GC GA  E RCLL L+F  L  I +A +   +L   F  D  K     
Sbjct: 61  GVGAITIVMGFLGCIGAVNEVRCLLGLYFVFLLLILIAQVTVGVLF-YFNADKLKKEMGN 119

Query: 399 LTEKLKTQYGSN--SAWTEFWDIVQTKFSCCG----VEFSKSHAISVFQKTRWYENSKPY 560
               +   Y +N  S+  E WD VQ +  CCG      ++++  +  F KT +  + +  
Sbjct: 120 TVMDIIRNYTANATSSREEAWDYVQAQVKCCGWVSHYNWTENEELMGFTKTTYPCSCEKI 179

Query: 561 KLPDSCCKMKGG--QIENVGICRQGNEN-GIHENGCMTVVMKSIGEQYIIVLSALITLAV 731
           K  D+   +K G  + +N  +     E+  ++  GCM      + E + I+L     +AV
Sbjct: 180 KEEDNQLIVKKGFCEADNSTVSENNPEDWPVNTEGCMEKAQAWLQENFGILLGVCAGVAV 239

Query: 732 VLIFGMCLSLAHLRNIINIRYNE 800
           + + G+ LS+   R I +  Y++
Sbjct: 240 IELLGLFLSICLCRYIHSEDYSK 262

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 128,552,960
Number of Sequences: 369166
Number of extensions: 2766788
Number of successful extensions: 7579
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7050
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7457
length of database: 68,354,980
effective HSP length: 112
effective length of database: 47,664,660
effective search space used: 12392811600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00588

  1. Dr_sW_015_G05
  2. Dr_sW_026_C13