Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_G05
(788 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q58CY8|TSN18_BOVIN Tetraspanin-18 (Tspan-18) 129 7e-30
sp|Q96SJ8|TSN18_HUMAN Tetraspanin-18 (Tspan-18) 126 8e-29
sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18) 124 2e-28
sp|O75954|TSN9_HUMAN Tetraspanin-9 (Tspan-9) (Tetraspan NET-5) 102 9e-22
sp|Q8BJU2|TSN9_MOUSE Tetraspanin-9 (Tspan-9) (Tetraspan NET-5) 100 3e-21
sp|P19075|TSN8_HUMAN Tetraspanin-8 (Tspan-8) (Transmembrane... 98 2e-20
sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell sur... 97 4e-20
sp|P27701|CD82_HUMAN CD82 antigen (Inducible membrane prote... 96 1e-19
sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell s... 96 1e-19
sp|O70352|CD82_RAT CD82 antigen (Metastasis suppressor homo... 96 1e-19
>sp|Q58CY8|TSN18_BOVIN Tetraspanin-18 (Tspan-18)
Length = 249
Score = 129 bits (325), Expect = 7e-30
Identities = 84/250 (33%), Positives = 134/250 (53%), Gaps = 10/250 (4%)
Frame = +3
Query: 63 SCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIG-YIVLGCGGTII 239
SC+K+L+ FNF + G + G+GIW+ V P+ E++ N I G YI+L GG +
Sbjct: 7 SCMKYLMFVFNFFIFLGGACLLGIGIWVMVDPTGFREIVAANPLLITGAYILLAMGGLLF 66
Query: 240 LISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLTEKLKT 419
L+ F GC GA EN+CLL FF + IF+A L A+IL IFR LT++ T++L
Sbjct: 67 LLGFLGCCGAVRENKCLLLFFFLFILIIFLAELSAAILAFIFR---GNLTREFFTKELTK 123
Query: 420 QY-GSN--SAWTEFWDIVQTKFSCCGVEFSKSHA-ISVFQKTRWYENSKPYKLPDSCCKM 587
Y GSN ++ W+ V F CCGV + SVF+ + ++P++CC+
Sbjct: 124 HYQGSNDTDVFSATWNSVMITFGCCGVNGPEDFKYASVFRLLTLDSD----EVPEACCRR 179
Query: 588 KGGQIENVGICRQ----GNENGIHENGCMTVVMKSIGEQYIIVLSAL-ITLAVVLIFGMC 752
+ + V + R+ G + +++ GC TV++ + E Y+ + AL I + + +F M
Sbjct: 180 EPQSRDGVLLSREECLLGRDLFLNKQGCYTVILNAF-ETYVYLAGALAIGVLAIELFAMI 238
Query: 753 LSLAHLRNII 782
++ R II
Sbjct: 239 FAMCLFRGII 248
>sp|Q96SJ8|TSN18_HUMAN Tetraspanin-18 (Tspan-18)
Length = 248
Score = 126 bits (316), Expect = 8e-29
Identities = 79/244 (32%), Positives = 129/244 (52%), Gaps = 13/244 (5%)
Frame = +3
Query: 63 SCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIG-YIVLGCGGTII 239
SC+K+L+ FNF + G + +GIW+ V P+ E++ N + G YI+L GG +
Sbjct: 7 SCMKYLMFVFNFFIFLGGACLLAIGIWVMVDPTGFREIVAANPLLLTGAYILLAMGGLLF 66
Query: 240 LISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLTEKLKT 419
L+ F GC GA EN+CLL FF + IF+A L A+IL IFR++L T++ T++L
Sbjct: 67 LLGFLGCCGAVRENKCLLLFFFLFILIIFLAELSAAILAFIFRENL---TREFFTKELTK 123
Query: 420 QYGSNSAWTEF---WDIVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDSCCKMK 590
Y N+ F W+ V F CCGV + + + ++ + +P++CC+ +
Sbjct: 124 HYQGNNDTDVFSATWNSVMITFGCCGVNGPEDFKFASVFRLLTLDSEE---VPEACCRRE 180
Query: 591 GGQIENVGICRQ----GNENGIHENGCMTVVMKSIGEQYIIVLSALI--TLAV---VLIF 743
+ V + R+ G +++ GC TV++ + E Y+ + AL LA+ +IF
Sbjct: 181 PQSRDGVLLSREECLLGRSLFLNKQGCYTVILNTF-ETYVYLAGALAIGVLAIELFAMIF 239
Query: 744 GMCL 755
MCL
Sbjct: 240 AMCL 243
>sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18)
Length = 248
Score = 124 bits (312), Expect = 2e-28
Identities = 80/244 (32%), Positives = 127/244 (52%), Gaps = 13/244 (5%)
Frame = +3
Query: 63 SCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIG-YIVLGCGGTII 239
SC+K+L+ FNF + G + G+GIW+ V P+ E++ N G YIVL GG +
Sbjct: 7 SCMKYLMFVFNFFVFLGGACLLGVGIWVLVDPTGFREIVATNPLLTTGAYIVLAMGGLLF 66
Query: 240 LISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLTEKLKT 419
L+ F GC GA ENRCLL FF + IF+ L A+IL IFR+ L T++ T++L
Sbjct: 67 LLGFLGCCGAVRENRCLLLFFFLFILIIFLVELSAAILAFIFREHL---TREFFTKELTK 123
Query: 420 QY-GSNSA--WTEFWDIVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDSCCKMK 590
Y G N ++ W+ V F CCGV + ++ + + + +P +CC+ +
Sbjct: 124 HYQGDNDTDVFSATWNSVMITFGCCGVNGPEDFKLASVFRLLTLDTEE---VPKACCRRE 180
Query: 591 GGQIENVGI----CRQGNENGIHENGCMTVVMKSIGEQYIIVLSALI--TLAV---VLIF 743
+ V + C+ G I++ GC TV++ + E Y+ + A LA+ +++F
Sbjct: 181 PQTRDGVVLSREECQLGRNPFINKQGCYTVILNTF-ETYVYLAGAFAIGVLAIELFLMVF 239
Query: 744 GMCL 755
MCL
Sbjct: 240 AMCL 243
>sp|O75954|TSN9_HUMAN Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)
Length = 239
Score = 102 bits (255), Expect = 9e-22
Identities = 65/246 (26%), Positives = 110/246 (44%), Gaps = 1/246 (0%)
Frame = +3
Query: 45 MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIGYIVLGC 224
M + C+K+++ FN +F +CG + G+GIW+ VS + +V+
Sbjct: 1 MARGCLCCLKYMMFLFNLIFWLCGCGLLGVGIWLSVSQGNFATFSPSFPSLSAANLVIAI 60
Query: 225 GGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLT 404
G +++ F GC GA EN+CLL FF VL I +A L+ IL ++ +++ K L
Sbjct: 61 GTIVMVTGFLGCLGAIKENKCLLLSFFIVLLVILLAELILLILFFVYMDKVNENAKKDLK 120
Query: 405 EKLKTQYGSNSAWTE-FWDIVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDSCC 581
E L + N+ + W+I+Q + CCGV T WY +PD CC
Sbjct: 121 EGLLLYHTENNVGLKNAWNIIQAEMRCCGVT----------DYTDWYPVLGENTVPDRCC 170
Query: 582 KMKGGQIENVGICRQGNENGIHENGCMTVVMKSIGEQYIIVLSALITLAVVLIFGMCLSL 761
+EN C + + GC V + ++ + + + ++ I GM S+
Sbjct: 171 ------MENSQGCGRNATTPLWRTGCYEKVKMWFDDNKHVLGTVGMCILIMQILGMAFSM 224
Query: 762 AHLRNI 779
++I
Sbjct: 225 TLFQHI 230
>sp|Q8BJU2|TSN9_MOUSE Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)
Length = 239
Score = 100 bits (250), Expect = 3e-21
Identities = 65/246 (26%), Positives = 109/246 (44%), Gaps = 1/246 (0%)
Frame = +3
Query: 45 MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIGYIVLGC 224
M + C+K+ + FN +F +CG + G+GIW+ VS + +V+
Sbjct: 1 MARGCLCCLKYTMFLFNLIFWLCGCGLLGVGIWLSVSQGNFATFSPSFPSLSAANLVIAI 60
Query: 225 GGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLT 404
G +++ F GC GA EN+CLL FF VL I +A L+ IL ++ +++ K L
Sbjct: 61 GTIVMVTGFLGCLGAIKENKCLLLSFFIVLLIILLAELILIILFFVYMDKVNENAKQDLK 120
Query: 405 EKLKTQYGSNSAWTE-FWDIVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDSCC 581
E L N+ + W+I+Q + CCGV T WY +PD CC
Sbjct: 121 EGLLLYNTENNVGLKNAWNIIQAEMRCCGVT----------DYTDWYPVLGENTVPDRCC 170
Query: 582 KMKGGQIENVGICRQGNENGIHENGCMTVVMKSIGEQYIIVLSALITLAVVLIFGMCLSL 761
+EN C + + + GC V + ++ + + + ++ I GM S+
Sbjct: 171 ------MENSQGCGRNSTTPLWRTGCYEKVKLWFDDNKHVLGTVGMCILIMQILGMAFSM 224
Query: 762 AHLRNI 779
++I
Sbjct: 225 TLFQHI 230
>sp|P19075|TSN8_HUMAN Tetraspanin-8 (Tspan-8) (Transmembrane 4 superfamily member 3)
(Tumor-associated antigen CO-029)
Length = 237
Score = 98.2 bits (243), Expect = 2e-20
Identities = 65/240 (27%), Positives = 113/240 (47%), Gaps = 7/240 (2%)
Frame = +3
Query: 63 SCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPIIGY----IVLGCGG 230
+C+K+ + FNFLF +CG+ I L IW+ VS + S + G Y I++ G
Sbjct: 6 ACIKYSMFTFNFLFWLCGILILALAIWVRVS-NDSQAIFGSEDVGSSSYVAVDILIAVGA 64
Query: 231 TIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQLTEK 410
I+++ F GC GA E+RC+L LFF L I + + IL ++F+ ++ + L E
Sbjct: 65 IIMILGFLGCCGAIKESRCMLLLFFIGLLLILLLQVATGILGAVFKSKSDRIVNETLYEN 124
Query: 411 LKTQYGSNSAWTEFWD---IVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDSCC 581
K + + +F + + Q +F CCG+ V W N + Y P+ C
Sbjct: 125 TKLLSATGESEKQFQEAIIVFQEEFKCCGL---------VNGAADWGNNFQHY--PELC- 172
Query: 582 KMKGGQIENVGICRQGNENGIHENGCMTVVMKSIGEQYIIVLSALITLAVVLIFGMCLSL 761
++ C+ N +++ C++ + + + IIV+ LAV+ I G+ S+
Sbjct: 173 ----ACLDKQRPCQSYNGKQVYKETCISFIKDFLAKNLIIVIGISFGLAVIEILGLVFSM 228
>sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
(Leukocyte antigen MRC OX-44)
Length = 219
Score = 97.4 bits (241), Expect = 4e-20
Identities = 51/153 (33%), Positives = 91/153 (59%), Gaps = 1/153 (0%)
Frame = +3
Query: 45 MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPI-IGYIVLG 221
MG + +K++L FFNFLF +CG I G GI + V + ++ +NL + +G +++
Sbjct: 1 MGMSSLKLLKYVLFFFNFLFWVCGCCILGFGIHLLVQNTYG--ILFRNLPFLTLGNVLVI 58
Query: 222 CGGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQL 401
G I++++F GC G+ EN+CLL FF +L I +A + +IL+ ++ K ++ L + L
Sbjct: 59 VGSIIMVVAFLGCMGSIKENKCLLMSFFVLLLLILLAEVTLAILLFVYEKKINTLVAEGL 118
Query: 402 TEKLKTQYGSNSAWTEFWDIVQTKFSCCGVEFS 500
+ ++ Y S+++ WD +Q++ CCGV S
Sbjct: 119 NDSIQ-HYHSDNSTRMAWDFIQSQLQCCGVNGS 150
>sp|P27701|CD82_HUMAN CD82 antigen (Inducible membrane protein R2) (C33 antigen) (IA4)
(Metastasis suppressor Kangai 1) (Suppressor of
tumorigenicity-6) (Tetraspanin-27) (Tspan-27)
Length = 267
Score = 95.9 bits (237), Expect = 1e-19
Identities = 72/270 (26%), Positives = 125/270 (46%), Gaps = 25/270 (9%)
Frame = +3
Query: 45 MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPII--GYIVL 218
MG K+ L FN +F I G I G G+W+ S+ ++ + + + Y+ +
Sbjct: 1 MGSACIKVTKYFLFLFNLIFFILGAVILGFGVWILADKSSFISVLQTSSSSLRMGAYVFI 60
Query: 219 GCGGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQ 398
G G +L+ F GC GA E RCLL L+F L I +A + A ++F ++ KL ++
Sbjct: 61 GVGAVTMLMGFLGCIGAVNEVRCLLGLYFAFLLLILIAQVTAG---ALFYFNMGKLKQEM 117
Query: 399 --LTEKLKTQYGSN--SAWTEFWDIVQTKFSCCG-VEFSKSHAISVFQKTRWYENSKPYK 563
+ +L Y S+ + + WD VQ + CCG V F W +N++
Sbjct: 118 GGIVTELIRDYNSSREDSLQDAWDYVQAQVKCCGWVSF-----------YNWTDNAELMN 166
Query: 564 LPD----SCCKMKGGQIENV----GIC-------RQGN---ENGIHENGCMTVVMKSIGE 689
P+ C++KG + ++ G C + GN + +++ GCM V + E
Sbjct: 167 RPEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWPVYQEGCMEKVQAWLQE 226
Query: 690 QYIIVLSALITLAVVLIFGMCLSLAHLRNI 779
I+L + +A++ + GM LS+ R++
Sbjct: 227 NLGIILGVGVGVAIIELLGMVLSICLCRHV 256
>sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
Length = 219
Score = 95.5 bits (236), Expect = 1e-19
Identities = 51/153 (33%), Positives = 90/153 (58%), Gaps = 1/153 (0%)
Frame = +3
Query: 45 MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPI-IGYIVLG 221
MG + +K++L FN LF +CG I G GI+ V + ++ +NL + +G I++
Sbjct: 1 MGMSSLKLLKYVLFIFNLLFWVCGCCILGFGIYFLVQNTYG--VLFRNLPFLTLGNILVI 58
Query: 222 CGGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQL 401
G I++++F GC G+ EN+CLL FF +L I +A + +IL+ ++ + L+ L + L
Sbjct: 59 VGSIIMVVAFLGCMGSIKENKCLLMSFFVLLLIILLAEVTIAILLFVYEQKLNTLVAEGL 118
Query: 402 TEKLKTQYGSNSAWTEFWDIVQTKFSCCGVEFS 500
+ ++ Y S+++ + WD +QT+ CCGV S
Sbjct: 119 NDSIQ-HYHSDNSTMKAWDFIQTQLQCCGVNGS 150
>sp|O70352|CD82_RAT CD82 antigen (Metastasis suppressor homolog)
Length = 266
Score = 95.5 bits (236), Expect = 1e-19
Identities = 72/260 (27%), Positives = 113/260 (43%), Gaps = 15/260 (5%)
Frame = +3
Query: 45 MGKKTFSCVKWLLTFFNFLFLICGLAICGLGIWMFVSPSTSNELIGKNLAPI-IG-YIVL 218
MG K+ L FN LF I G I G G+W+ S+ ++ + + + +G Y+ +
Sbjct: 1 MGAGCVKVTKYFLFLFNLLFFILGAVILGFGVWILADKSSFISVLQTSSSSLQVGAYVFI 60
Query: 219 GCGGTIILISFCGCAGAGLENRCLLCLFFTVLCGIFVACLVASILVSIFRKDLSKLTKDQ 398
G G +L+ F GC GA E RCLL L+F L I +A + +L L + +
Sbjct: 61 GVGAITMLMGFLGCIGAVNEVRCLLGLYFVFLLLILIAQVTVEVLFYFNANKLKQEMGNT 120
Query: 399 LTEKLKT-QYGSNSAWTEFWDIVQTKFSCCGVEFSKSHAISVFQKTRWYENSKPYKLPDS 575
+ + ++ ++S+ E WD VQ + CCG + S + + +NS P S
Sbjct: 121 VMDIIQNYSVNASSSREEAWDYVQAQVKCCGWV-----SPSNWTRNPVLKNSTKTTYPCS 175
Query: 576 CCKMK---GGQIENVGICRQGNENG---------IHENGCMTVVMKSIGEQYIIVLSALI 719
C K K I G C N +H GCM + E + I+L
Sbjct: 176 CEKTKEEDNQLIVKKGFCESDNSTASENSPEDWPVHPEGCMEKAQAWLQENFGILLGVCA 235
Query: 720 TLAVVLIFGMCLSLAHLRNI 779
+AV+ + G+ LS+ R I
Sbjct: 236 GVAVIELLGLFLSICLCRYI 255
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,450,639
Number of Sequences: 369166
Number of extensions: 2063254
Number of successful extensions: 6404
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5997
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6282
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7454154400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)