Planaria EST Database


DrC_00448

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00448
         (975 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P09194|PHLC_TRYBB  Variant-surface-glycoprotein phospholi...    84   6e-16
sp|O15886|PHLC_TRYCR  Variant-surface-glycoprotein phospholi...    75   3e-13
sp|P34024|PLC_LISMO  1-phosphatidylinositol phosphodiesteras...    56   1e-07
sp|P14262|PLC_BACCE  1-phosphatidylinositol phosphodiesteras...    49   3e-05
sp|P08954|PLC_BACTU  1-phosphatidylinositol phosphodiesteras...    48   4e-05
sp|P45723|PLC_STAAU  1-phosphatidylinositol phosphodiesteras...    45   3e-04
sp|O07664|HPRK_ENTFA  HPr kinase/phosphorylase (HPrK/P) (HPr...    32   2.7  
sp|P44207|YE76_HAEIN  Putative HTH-type transcriptional regu...    31   6.0  
sp|Q03306|PKH3_YEAST  Serine/threonine-protein kinase PKH3 (...    31   6.0  
sp|P13010|KU86_HUMAN  ATP-dependent DNA helicase II, 80 kDa ...    30   7.9  
>sp|P09194|PHLC_TRYBB Variant-surface-glycoprotein phospholipase C (VSG lipase)
           (Glycosylphosphatidylinositol-specific phospholipase C)
           (GPI-PLC)
          Length = 358

 Score = 84.0 bits (206), Expect = 6e-16
 Identities = 65/233 (27%), Positives = 112/233 (48%), Gaps = 14/233 (6%)
 Frame = +3

Query: 87  YNPSDWMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKL----ISIF 254
           ++P  WMS+   ++ K  +  + + G H++ T+ I   S    DA + +  L      + 
Sbjct: 7   WSPQSWMSDTRSSIEKKCIGQVYMVGAHNAGTHGIQMFSPFGLDAPEKLRSLPPYVTFLL 66

Query: 255 GSWAVSVISRWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLC 434
               V V SRW + Q+L     L+ G+RY D+R  I+    N+IY+ H   S  +++ L 
Sbjct: 67  RFLTVGVSSRWGRCQNLSIRQLLDHGVRYLDLRMNISPDQENKIYTTHFHISVPLQEVLK 126

Query: 435 AINNFLKT--HQKEVVLLDLNHFYNFTQ-HDHMRLLHYLETIFSDKLVPYSRTIPT-LSE 602
            + +FL T     E V+LD  HFY F + H   R +  L+ +  +  +P + ++ T L  
Sbjct: 127 DVKDFLTTPASANEFVILDFLHFYGFNESHTMKRFVEELQAL-EEFYIPTTVSLTTPLCN 185

Query: 603 LWETNHRVFVFYHCENVHKPYLWPGY------TIPSPWPNTDKLSMLMKFLSE 743
           LW++  R+F+      V +PY+   Y       + S W N  +L+ L+  L E
Sbjct: 186 LWQSTRRIFL------VVRPYVEYPYARLRSVALKSIWVNQMELNDLLDRLEE 232
>sp|O15886|PHLC_TRYCR Variant-surface-glycoprotein phospholipase C (VSG lipase)
           (Glycosylphosphatidylinositol-specific phospholipase C)
           (GPI-PLC)
          Length = 380

 Score = 75.1 bits (183), Expect = 3e-13
 Identities = 57/229 (24%), Positives = 97/229 (42%), Gaps = 12/229 (5%)
 Frame = +3

Query: 87  YNPSDWMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWA 266
           ++P  WM +L   + ++ +  +   G+HD+ +Y ++  S    DA   ++   S+F S  
Sbjct: 13  WHPQSWMHDLRSFIGEMAITQVCFVGSHDAASYGVSKDSPFGADAPGFLLG-DSVFASLL 71

Query: 267 V----SVISRWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLC 434
                 + + W++ Q +    QLN G+RY D+R A N  D + +Y+ H   S  + D L 
Sbjct: 72  RFLFRGICASWSRCQWMSVRAQLNHGVRYLDMRVATNPEDASRLYTLHHQISVPLADVLE 131

Query: 435 AINNFLK--THQKEVVLLDLNHFYNFTQHDHMRLLHYLETIFSDKLVPYSRTIPT-LSEL 605
            +  FL       E ++LD  H Y     D            SD+ +P    + T L  L
Sbjct: 132 DVKAFLNDPLSADEFIVLDFQHLYLTDDSDGKGKFFRELDRLSDRFIPVDVPLTTPLEFL 191

Query: 606 WETNHRVFVFYHCENVHKPYLWPGYTIPSP-----WPNTDKLSMLMKFL 737
           W  + R  +F    +      +P   I S      W N + L  L++ L
Sbjct: 192 WRASSRRRIFLVVGSGEDESPYPAARIRSKCMVSRWVNENSLRKLLEAL 240
>sp|P34024|PLC_LISMO 1-phosphatidylinositol phosphodiesterase precursor
           (Phosphatidylinositol diacylglycerol-lyase)
           (Phosphatidylinositol-specific phospholipase C) (PI-PLC)
          Length = 317

 Score = 56.2 bits (134), Expect = 1e-07
 Identities = 47/172 (27%), Positives = 71/172 (41%), Gaps = 4/172 (2%)
 Frame = +3

Query: 102 WMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAVSVIS 281
           WMS LP+T     L  ++IPGTHD+ +Y                       G    ++  
Sbjct: 48  WMSALPDTTN---LAALSIPGTHDTMSYN----------------------GDITWTLTK 82

Query: 282 RWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNFLKTH 461
             A+ Q +  Y QL AGIRY DIR+    +D   IY      + ++   L  I  FLK +
Sbjct: 83  PLAQTQTMSLYQQLEAGIRYIDIRA----KDNLNIYHGPIFLNASLSGVLETITQFLKKN 138

Query: 462 QKEVVLLDLNHFYNFTQHDHMRLLH----YLETIFSDKLVPYSRTIPTLSEL 605
            KE +++ L    N       R+      Y +  ++      S  IPTL ++
Sbjct: 139 PKETIIMRLKDEQNSNDSFDYRIQPLINIYKDYFYTTPRTDTSNKIPTLKDV 190
>sp|P14262|PLC_BACCE 1-phosphatidylinositol phosphodiesterase precursor
           (Phosphatidylinositol diacylglycerol-lyase)
           (Phosphatidylinositol-specific phospholipase C) (PI-PLC)
          Length = 329

 Score = 48.5 bits (114), Expect = 3e-05
 Identities = 36/137 (26%), Positives = 59/137 (43%)
 Frame = +3

Query: 90  NPSDWMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAV 269
           N S WM  +P+++   PL  I+IPGTHDS T+K+ +            IK +        
Sbjct: 40  NWSKWMQPIPDSI---PLARISIPGTHDSGTFKLQN-----------PIKQV-------- 77

Query: 270 SVISRWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNF 449
                W   Q+ D   Q++ G R FDIR  + D +   ++         + + +     F
Sbjct: 78  -----WGMTQEYDFRYQMDHGARIFDIRGRLTDDNTIVLHHGPLYLYVTLHEFINEAKQF 132

Query: 450 LKTHQKEVVLLDLNHFY 500
           LK +  E +++ L   Y
Sbjct: 133 LKDNPSETIIMSLKKEY 149
>sp|P08954|PLC_BACTU 1-phosphatidylinositol phosphodiesterase precursor
           (Phosphatidylinositol diacylglycerol-lyase)
           (Phosphatidylinositol-specific phospholipase C) (PI-PLC)
          Length = 329

 Score = 48.1 bits (113), Expect = 4e-05
 Identities = 36/137 (26%), Positives = 58/137 (42%)
 Frame = +3

Query: 90  NPSDWMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAV 269
           N S WM  +P+ +   PL  I+IPGTHDS T+K+ +            IK +        
Sbjct: 40  NWSKWMQPIPDNI---PLARISIPGTHDSGTFKLQN-----------PIKQV-------- 77

Query: 270 SVISRWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNF 449
                W   Q+ D   Q++ G R FDIR  + D +   ++         + + +     F
Sbjct: 78  -----WGMTQEYDFRYQMDHGARIFDIRGRLTDDNTIVLHHGPLYLYVTLHEFINEAKQF 132

Query: 450 LKTHQKEVVLLDLNHFY 500
           LK +  E +++ L   Y
Sbjct: 133 LKDNPSETIIMSLKKEY 149
>sp|P45723|PLC_STAAU 1-phosphatidylinositol phosphodiesterase precursor
           (Phosphatidylinositol diacylglycerol-lyase)
           (Phosphatidylinositol-specific phospholipase C) (PI-PLC)
          Length = 311

 Score = 45.1 bits (105), Expect = 3e-04
 Identities = 47/182 (25%), Positives = 71/182 (39%), Gaps = 11/182 (6%)
 Frame = +3

Query: 90  NPSDWMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAV 269
           +P +WMS L +      +N   IPG+HDS ++ +                          
Sbjct: 17  SPENWMSKLDDGKHLTEIN---IPGSHDSGSFTLKD------------------------ 49

Query: 270 SVISRWAKAQDLDPYGQLNAGIRYFDIR---SAIN--DRDGNEIYSCHSLFSGNIRDDLC 434
            V S WAK QD D   Q+ +G+R+FDIR   SA N        +Y  H L  G   DD  
Sbjct: 50  PVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMISVHHGMVYLHHEL--GKFLDD-- 105

Query: 435 AINNFLKTHQKEVVLLDLNHFYNF------TQHDHMRLLHYLETIFSDKLVPYSRTIPTL 596
               +L  +  E +++ +   Y+       T  +  R  +Y    + +     S   PTL
Sbjct: 106 -AKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYYYNNPQYQNLFYTGSNANPTL 164

Query: 597 SE 602
            E
Sbjct: 165 KE 166
>sp|O07664|HPRK_ENTFA HPr kinase/phosphorylase (HPrK/P) (HPr(Ser) kinase/phosphorylase)
          Length = 311

 Score = 32.0 bits (71), Expect = 2.7
 Identities = 13/51 (25%), Positives = 29/51 (56%)
 Frame = +3

Query: 435 AINNFLKTHQKEVVLLDLNHFYNFTQHDHMRLLHYLETIFSDKLVPYSRTI 587
           ++N  +KT +     L+L  ++N+  HD ++L    E  F+++++P  R +
Sbjct: 25  SLNRTIKTGEISRPGLELTGYFNYYSHDRLQLFGSKEITFAERMMPEERLL 75
>sp|P44207|YE76_HAEIN Putative HTH-type transcriptional regulator HI1476
          Length = 239

 Score = 30.8 bits (68), Expect = 6.0
 Identities = 20/69 (28%), Positives = 32/69 (46%), Gaps = 6/69 (8%)
 Frame = +3

Query: 303 LDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAI-----NNFLKT-HQ 464
           +D Y  +N    +      I   DG E YS   L S  ++ D CA+     N+ L T + 
Sbjct: 104 IDSYSSINVSAGFGSFNEGITQPDGQEPYSDELLTSLGVKADNCAVFWANGNSMLPTINN 163

Query: 465 KEVVLLDLN 491
            + +L+DL+
Sbjct: 164 YDQMLVDLS 172
>sp|Q03306|PKH3_YEAST Serine/threonine-protein kinase PKH3 (Pkb-activating kinase homolog
           3)
          Length = 898

 Score = 30.8 bits (68), Expect = 6.0
 Identities = 14/36 (38%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
 Frame = +3

Query: 45  FSRFTRKSYVHLQNYNPSDWMSNLP--ETLTKLPLN 146
           F+  TRK ++ L+ Y    W  +LP   TL+K+P N
Sbjct: 601 FNELTRKGFLILEKYKNRIWFISLPSYSTLSKIPFN 636
>sp|P13010|KU86_HUMAN ATP-dependent DNA helicase II, 80 kDa subunit (Lupus Ku autoantigen
           protein p86) (Ku86) (Ku80) (86 kDa subunit of Ku
           antigen) (Thyroid-lupus autoantigen) (TLAA) (CTC box
           binding factor 85 kDa subunit) (CTCBF) (CTC85) (Nuclear
           factor IV) (DNA-repair protein XRCC5)
          Length = 732

 Score = 30.4 bits (67), Expect = 7.9
 Identities = 13/31 (41%), Positives = 19/31 (61%)
 Frame = +3

Query: 441 NNFLKTHQKEVVLLDLNHFYNFTQHDHMRLL 533
           NNFLK  Q++V +  LNHF+     D + L+
Sbjct: 656 NNFLKALQEKVEIKQLNHFWEIVVQDGITLI 686
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 122,324,432
Number of Sequences: 369166
Number of extensions: 2686501
Number of successful extensions: 6197
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5947
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6192
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10191921135
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00448

  1. Dr_sW_011_M20
  2. Dr_sW_027_C18