Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_C18
(869 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P09194|PHLC_TRYBB Variant-surface-glycoprotein phospholi... 79 1e-14
sp|O15886|PHLC_TRYCR Variant-surface-glycoprotein phospholi... 75 3e-13
sp|P34024|PLC_LISMO 1-phosphatidylinositol phosphodiesteras... 50 8e-06
sp|P45723|PLC_STAAU 1-phosphatidylinositol phosphodiesteras... 44 8e-04
sp|P08954|PLC_BACTU 1-phosphatidylinositol phosphodiesteras... 42 0.002
sp|P14262|PLC_BACCE 1-phosphatidylinositol phosphodiesteras... 42 0.002
sp|O07664|HPRK_ENTFA HPr kinase/phosphorylase (HPrK/P) (HPr... 32 2.3
sp|P44207|YE76_HAEIN Putative HTH-type transcriptional regu... 31 5.1
sp|P13010|KU86_HUMAN ATP-dependent DNA helicase II, 80 kDa ... 30 6.7
sp|Q62967|ERG19_RAT Diphosphomevalonate decarboxylase (Meva... 30 6.7
>sp|P09194|PHLC_TRYBB Variant-surface-glycoprotein phospholipase C (VSG lipase)
(Glycosylphosphatidylinositol-specific phospholipase C)
(GPI-PLC)
Length = 358
Score = 79.3 bits (194), Expect = 1e-14
Identities = 70/260 (26%), Positives = 117/260 (45%), Gaps = 21/260 (8%)
Frame = +3
Query: 3 SNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKL----ISIFGSWAVSV 170
S+ ++ K + + + G H++ T+ I S DA + + L + V V
Sbjct: 14 SDTRSSIEKKCIGQVYMVGAHNAGTHGIQMFSPFGLDAPEKLRSLPPYVTFLLRFLTVGV 73
Query: 171 ISRWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNFLK 350
SRW + Q+L L+ G+RY D+R I+ N+IY+ H S +++ L + +FL
Sbjct: 74 SSRWGRCQNLSIRQLLDHGVRYLDLRMNISPDQENKIYTTHFHISVPLQEVLKDVKDFLT 133
Query: 351 T--HQKEVVLLDLNHFYNFTQ-HDHMRLLHYLETIFSDKLVPYSRTIPT-LSELWETNHR 518
T E V+LD HFY F + H R + L+ + + +P + ++ T L LW++ R
Sbjct: 134 TPASANEFVILDFLHFYGFNESHTMKRFVEELQAL-EEFYIPTTVSLTTPLCNLWQSTRR 192
Query: 519 VFVFYHCENVHKPYLWPGY------TIPSPWPN-------TDKLSMLMKFLSENYKEGHF 659
+F+ V +PY+ Y + S W N D+L LM E+ G
Sbjct: 193 IFL------VVRPYVEYPYARLRSVALKSIWVNQMELNDLLDRLEELMTRDLEDVSIGGV 246
Query: 660 GDYFYICQGVLTPTPKDYFV 719
Y+ Q + TP D+ V
Sbjct: 247 PSKMYVTQAIGTPRNNDFAV 266
>sp|O15886|PHLC_TRYCR Variant-surface-glycoprotein phospholipase C (VSG lipase)
(Glycosylphosphatidylinositol-specific phospholipase C)
(GPI-PLC)
Length = 380
Score = 74.7 bits (182), Expect = 3e-13
Identities = 65/284 (22%), Positives = 112/284 (39%), Gaps = 25/284 (8%)
Frame = +3
Query: 6 NLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAV----SVI 173
+L + ++ + + G+HD+ +Y ++ S DA ++ S+F S +
Sbjct: 21 DLRSFIGEMAITQVCFVGSHDAASYGVSKDSPFGADAPGFLLG-DSVFASLLRFLFRGIC 79
Query: 174 SRWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNFLK- 350
+ W++ Q + QLN G+RY D+R A N D + +Y+ H S + D L + FL
Sbjct: 80 ASWSRCQWMSVRAQLNHGVRYLDMRVATNPEDASRLYTLHHQISVPLADVLEDVKAFLND 139
Query: 351 -THQKEVVLLDLNHFYNFTQHDHMRLLHYLETIFSDKLVPYSRTIPT-LSELWETNHRVF 524
E ++LD H Y D SD+ +P + T L LW + R
Sbjct: 140 PLSADEFIVLDFQHLYLTDDSDGKGKFFRELDRLSDRFIPVDVPLTTPLEFLWRASSRRR 199
Query: 525 VFYHCENVHKPYLWPGYTIPSP-----WPNTDKLSMLMKFLSE------NYKEGHFGDYF 671
+F + +P I S W N + L L++ L Y +
Sbjct: 200 IFLVVGSGEDESPYPAARIRSKCMVSRWVNENSLRKLLEALDNLLLDDLKYPQTGVPSKL 259
Query: 672 YICQGVLTPTPKDYFVHMMESLKT-------VMASKATPGFAKW 782
Y+ Q V TP D F+ + + +A++ P +W
Sbjct: 260 YVTQAVFTPRYSDIFLGIFPKISRRVVSSIYDVATRVNPSLLEW 303
>sp|P34024|PLC_LISMO 1-phosphatidylinositol phosphodiesterase precursor
(Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol-specific phospholipase C) (PI-PLC)
Length = 317
Score = 50.1 bits (118), Expect = 8e-06
Identities = 45/170 (26%), Positives = 69/170 (40%), Gaps = 4/170 (2%)
Frame = +3
Query: 3 SNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAVSVISRW 182
S LP+T L ++IPGTHD+ +Y G ++
Sbjct: 50 SALPDTTN---LAALSIPGTHDTMSYN----------------------GDITWTLTKPL 84
Query: 183 AKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNFLKTHQK 362
A+ Q + Y QL AGIRY DIR+ +D IY + ++ L I FLK + K
Sbjct: 85 AQTQTMSLYQQLEAGIRYIDIRA----KDNLNIYHGPIFLNASLSGVLETITQFLKKNPK 140
Query: 363 EVVLLDLNHFYNFTQHDHMRLLH----YLETIFSDKLVPYSRTIPTLSEL 500
E +++ L N R+ Y + ++ S IPTL ++
Sbjct: 141 ETIIMRLKDEQNSNDSFDYRIQPLINIYKDYFYTTPRTDTSNKIPTLKDV 190
>sp|P45723|PLC_STAAU 1-phosphatidylinositol phosphodiesterase precursor
(Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol-specific phospholipase C) (PI-PLC)
Length = 311
Score = 43.5 bits (101), Expect = 8e-04
Identities = 76/318 (23%), Positives = 120/318 (37%), Gaps = 43/318 (13%)
Frame = +3
Query: 15 ETLTKLPLNWIA------------IPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSW 158
++L+K P NW++ IPG+HDS ++ +
Sbjct: 12 DSLSKSPENWMSKLDDGKHLTEINIPGSHDSGSFTLKD---------------------- 49
Query: 159 AVSVISRWAKAQDLDPYGQLNAGIRYFDIR---SAIN--DRDGNEIYSCHSLFSGNIRDD 323
V S WAK QD D Q+ +G+R+FDIR SA N +Y H L G DD
Sbjct: 50 --PVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMISVHHGMVYLHHEL--GKFLDD 105
Query: 324 LCAINNFLKTHQKEVVLLDLNHFYNF------TQHDHMRLLHYLETIFSDKLVPYSRTIP 485
+L + E +++ + Y+ T + R +Y + + S P
Sbjct: 106 ---AKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYYYNNPQYQNLFYTGSNANP 162
Query: 486 TLSELWETNHRVFVFYHCENVHKPYLWPGY---TIPSPWPNTDKLSMLMKFLSENYK-EG 653
TL ET ++ +F + Y+ GY T W + + S N K +
Sbjct: 163 TLK---ETKGKIVLF---NRMGGTYIKSGYGADTSGIQWADNATFETKINNGSLNLKVQD 216
Query: 654 HFGDYF--------YICQGVLTPTPKD-YFVHMM------ESLKTV-MASKATPGFAKWL 785
+ DY+ + + T + KD +V+ + L T AS P AK +
Sbjct: 217 EYKDYYDKKVEAVKNLLLKLKTDSNKDNVYVNFLSVASGGSDLSTYNYASHINPEIAKTI 276
Query: 786 KDKEIGKGKINCSIVDFA 839
K GK + IVD+A
Sbjct: 277 KAN--GKARTGWLIVDYA 292
>sp|P08954|PLC_BACTU 1-phosphatidylinositol phosphodiesterase precursor
(Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol-specific phospholipase C) (PI-PLC)
Length = 329
Score = 42.0 bits (97), Expect = 0.002
Identities = 31/122 (25%), Positives = 51/122 (41%)
Frame = +3
Query: 30 LPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAVSVISRWAKAQDLDPY 209
+PL I+IPGTHDS T+K+ + IK + W Q+ D
Sbjct: 52 IPLARISIPGTHDSGTFKLQN-----------PIKQV-------------WGMTQEYDFR 87
Query: 210 GQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNFLKTHQKEVVLLDLNH 389
Q++ G R FDIR + D + ++ + + + FLK + E +++ L
Sbjct: 88 YQMDHGARIFDIRGRLTDDNTIVLHHGPLYLYVTLHEFINEAKQFLKDNPSETIIMSLKK 147
Query: 390 FY 395
Y
Sbjct: 148 EY 149
>sp|P14262|PLC_BACCE 1-phosphatidylinositol phosphodiesterase precursor
(Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol-specific phospholipase C) (PI-PLC)
Length = 329
Score = 42.0 bits (97), Expect = 0.002
Identities = 31/122 (25%), Positives = 51/122 (41%)
Frame = +3
Query: 30 LPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAVSVISRWAKAQDLDPY 209
+PL I+IPGTHDS T+K+ + IK + W Q+ D
Sbjct: 52 IPLARISIPGTHDSGTFKLQN-----------PIKQV-------------WGMTQEYDFR 87
Query: 210 GQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNFLKTHQKEVVLLDLNH 389
Q++ G R FDIR + D + ++ + + + FLK + E +++ L
Sbjct: 88 YQMDHGARIFDIRGRLTDDNTIVLHHGPLYLYVTLHEFINEAKQFLKDNPSETIIMSLKK 147
Query: 390 FY 395
Y
Sbjct: 148 EY 149
>sp|O07664|HPRK_ENTFA HPr kinase/phosphorylase (HPrK/P) (HPr(Ser) kinase/phosphorylase)
Length = 311
Score = 32.0 bits (71), Expect = 2.3
Identities = 13/51 (25%), Positives = 29/51 (56%)
Frame = +3
Query: 330 AINNFLKTHQKEVVLLDLNHFYNFTQHDHMRLLHYLETIFSDKLVPYSRTI 482
++N +KT + L+L ++N+ HD ++L E F+++++P R +
Sbjct: 25 SLNRTIKTGEISRPGLELTGYFNYYSHDRLQLFGSKEITFAERMMPEERLL 75
>sp|P44207|YE76_HAEIN Putative HTH-type transcriptional regulator HI1476
Length = 239
Score = 30.8 bits (68), Expect = 5.1
Identities = 20/69 (28%), Positives = 32/69 (46%), Gaps = 6/69 (8%)
Frame = +3
Query: 198 LDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAI-----NNFLKT-HQ 359
+D Y +N + I DG E YS L S ++ D CA+ N+ L T +
Sbjct: 104 IDSYSSINVSAGFGSFNEGITQPDGQEPYSDELLTSLGVKADNCAVFWANGNSMLPTINN 163
Query: 360 KEVVLLDLN 386
+ +L+DL+
Sbjct: 164 YDQMLVDLS 172
>sp|P13010|KU86_HUMAN ATP-dependent DNA helicase II, 80 kDa subunit (Lupus Ku autoantigen
protein p86) (Ku86) (Ku80) (86 kDa subunit of Ku
antigen) (Thyroid-lupus autoantigen) (TLAA) (CTC box
binding factor 85 kDa subunit) (CTCBF) (CTC85) (Nuclear
factor IV) (DNA-repair protein XRCC5)
Length = 732
Score = 30.4 bits (67), Expect = 6.7
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +3
Query: 336 NNFLKTHQKEVVLLDLNHFYNFTQHDHMRLL 428
NNFLK Q++V + LNHF+ D + L+
Sbjct: 656 NNFLKALQEKVEIKQLNHFWEIVVQDGITLI 686
>sp|Q62967|ERG19_RAT Diphosphomevalonate decarboxylase (Mevalonate pyrophosphate
decarboxylase) (Mevalonate (diphospho)decarboxylase)
Length = 401
Score = 30.4 bits (67), Expect = 6.7
Identities = 27/117 (23%), Positives = 51/117 (43%), Gaps = 6/117 (5%)
Frame = +3
Query: 477 TIPTLSELWETNHRVFVFYHCENVHKPYLWPGYTIPSPWPNT------DKLSMLMKFLSE 638
T P +S L +T+ R+ H N H YT + PN D ++ + +
Sbjct: 271 TFPPISYLNDTSRRIIQLVHRFNAHHGQTKVAYTFDAG-PNAVIFTLEDTVAEFVAAVRH 329
Query: 639 NYKEGHFGDYFYICQGVLTPTPKDYFVHMMESLKTVMASKATPGFAKWLKDKEIGKG 809
++ GD F +G+ V + + LKT +A++ +PG +++ ++G G
Sbjct: 330 SFPPAANGDKFL--KGLQVAP-----VLLSDELKTSLATEPSPGGVQYIIATQVGPG 379
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109,125,529
Number of Sequences: 369166
Number of extensions: 2364340
Number of successful extensions: 5453
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5449
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8598109270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)