Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_M20
(850 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P09194|PHLC_TRYBB Variant-surface-glycoprotein phospholi... 84 5e-16
sp|O15886|PHLC_TRYCR Variant-surface-glycoprotein phospholi... 75 2e-13
sp|P34024|PLC_LISMO 1-phosphatidylinositol phosphodiesteras... 56 1e-07
sp|P14262|PLC_BACCE 1-phosphatidylinositol phosphodiesteras... 49 2e-05
sp|P08954|PLC_BACTU 1-phosphatidylinositol phosphodiesteras... 48 3e-05
sp|P45723|PLC_STAAU 1-phosphatidylinositol phosphodiesteras... 45 3e-04
sp|O07664|HPRK_ENTFA HPr kinase/phosphorylase (HPrK/P) (HPr... 32 2.2
sp|P44207|YE76_HAEIN Putative HTH-type transcriptional regu... 31 5.0
sp|Q03306|PKH3_YEAST Serine/threonine-protein kinase PKH3 (... 31 5.0
sp|P13010|KU86_HUMAN ATP-dependent DNA helicase II, 80 kDa ... 30 6.5
>sp|P09194|PHLC_TRYBB Variant-surface-glycoprotein phospholipase C (VSG lipase)
(Glycosylphosphatidylinositol-specific phospholipase C)
(GPI-PLC)
Length = 358
Score = 84.0 bits (206), Expect = 5e-16
Identities = 65/233 (27%), Positives = 112/233 (48%), Gaps = 14/233 (6%)
Frame = +3
Query: 87 YNPSDWMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKL----ISIF 254
++P WMS+ ++ K + + + G H++ T+ I S DA + + L +
Sbjct: 7 WSPQSWMSDTRSSIEKKCIGQVYMVGAHNAGTHGIQMFSPFGLDAPEKLRSLPPYVTFLL 66
Query: 255 GSWAVSVISRWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLC 434
V V SRW + Q+L L+ G+RY D+R I+ N+IY+ H S +++ L
Sbjct: 67 RFLTVGVSSRWGRCQNLSIRQLLDHGVRYLDLRMNISPDQENKIYTTHFHISVPLQEVLK 126
Query: 435 AINNFLKT--HQKEVVLLDLNHFYNFTQ-HDHMRLLHYLETIFSDKLVPYSRTIPT-LSE 602
+ +FL T E V+LD HFY F + H R + L+ + + +P + ++ T L
Sbjct: 127 DVKDFLTTPASANEFVILDFLHFYGFNESHTMKRFVEELQAL-EEFYIPTTVSLTTPLCN 185
Query: 603 LWETNHRVFVFYHCENVHKPYLWPGY------TIPSPWPNTDKLSMLMKFLSE 743
LW++ R+F+ V +PY+ Y + S W N +L+ L+ L E
Sbjct: 186 LWQSTRRIFL------VVRPYVEYPYARLRSVALKSIWVNQMELNDLLDRLEE 232
>sp|O15886|PHLC_TRYCR Variant-surface-glycoprotein phospholipase C (VSG lipase)
(Glycosylphosphatidylinositol-specific phospholipase C)
(GPI-PLC)
Length = 380
Score = 75.1 bits (183), Expect = 2e-13
Identities = 57/229 (24%), Positives = 97/229 (42%), Gaps = 12/229 (5%)
Frame = +3
Query: 87 YNPSDWMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWA 266
++P WM +L + ++ + + G+HD+ +Y ++ S DA ++ S+F S
Sbjct: 13 WHPQSWMHDLRSFIGEMAITQVCFVGSHDAASYGVSKDSPFGADAPGFLLG-DSVFASLL 71
Query: 267 V----SVISRWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLC 434
+ + W++ Q + QLN G+RY D+R A N D + +Y+ H S + D L
Sbjct: 72 RFLFRGICASWSRCQWMSVRAQLNHGVRYLDMRVATNPEDASRLYTLHHQISVPLADVLE 131
Query: 435 AINNFLK--THQKEVVLLDLNHFYNFTQHDHMRLLHYLETIFSDKLVPYSRTIPT-LSEL 605
+ FL E ++LD H Y D SD+ +P + T L L
Sbjct: 132 DVKAFLNDPLSADEFIVLDFQHLYLTDDSDGKGKFFRELDRLSDRFIPVDVPLTTPLEFL 191
Query: 606 WETNHRVFVFYHCENVHKPYLWPGYTIPSP-----WPNTDKLSMLMKFL 737
W + R +F + +P I S W N + L L++ L
Sbjct: 192 WRASSRRRIFLVVGSGEDESPYPAARIRSKCMVSRWVNENSLRKLLEAL 240
>sp|P34024|PLC_LISMO 1-phosphatidylinositol phosphodiesterase precursor
(Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol-specific phospholipase C) (PI-PLC)
Length = 317
Score = 56.2 bits (134), Expect = 1e-07
Identities = 47/172 (27%), Positives = 71/172 (41%), Gaps = 4/172 (2%)
Frame = +3
Query: 102 WMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAVSVIS 281
WMS LP+T L ++IPGTHD+ +Y G ++
Sbjct: 48 WMSALPDTTN---LAALSIPGTHDTMSYN----------------------GDITWTLTK 82
Query: 282 RWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNFLKTH 461
A+ Q + Y QL AGIRY DIR+ +D IY + ++ L I FLK +
Sbjct: 83 PLAQTQTMSLYQQLEAGIRYIDIRA----KDNLNIYHGPIFLNASLSGVLETITQFLKKN 138
Query: 462 QKEVVLLDLNHFYNFTQHDHMRLLH----YLETIFSDKLVPYSRTIPTLSEL 605
KE +++ L N R+ Y + ++ S IPTL ++
Sbjct: 139 PKETIIMRLKDEQNSNDSFDYRIQPLINIYKDYFYTTPRTDTSNKIPTLKDV 190
>sp|P14262|PLC_BACCE 1-phosphatidylinositol phosphodiesterase precursor
(Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol-specific phospholipase C) (PI-PLC)
Length = 329
Score = 48.5 bits (114), Expect = 2e-05
Identities = 36/137 (26%), Positives = 59/137 (43%)
Frame = +3
Query: 90 NPSDWMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAV 269
N S WM +P+++ PL I+IPGTHDS T+K+ + IK +
Sbjct: 40 NWSKWMQPIPDSI---PLARISIPGTHDSGTFKLQN-----------PIKQV-------- 77
Query: 270 SVISRWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNF 449
W Q+ D Q++ G R FDIR + D + ++ + + + F
Sbjct: 78 -----WGMTQEYDFRYQMDHGARIFDIRGRLTDDNTIVLHHGPLYLYVTLHEFINEAKQF 132
Query: 450 LKTHQKEVVLLDLNHFY 500
LK + E +++ L Y
Sbjct: 133 LKDNPSETIIMSLKKEY 149
>sp|P08954|PLC_BACTU 1-phosphatidylinositol phosphodiesterase precursor
(Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol-specific phospholipase C) (PI-PLC)
Length = 329
Score = 48.1 bits (113), Expect = 3e-05
Identities = 36/137 (26%), Positives = 58/137 (42%)
Frame = +3
Query: 90 NPSDWMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAV 269
N S WM +P+ + PL I+IPGTHDS T+K+ + IK +
Sbjct: 40 NWSKWMQPIPDNI---PLARISIPGTHDSGTFKLQN-----------PIKQV-------- 77
Query: 270 SVISRWAKAQDLDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAINNF 449
W Q+ D Q++ G R FDIR + D + ++ + + + F
Sbjct: 78 -----WGMTQEYDFRYQMDHGARIFDIRGRLTDDNTIVLHHGPLYLYVTLHEFINEAKQF 132
Query: 450 LKTHQKEVVLLDLNHFY 500
LK + E +++ L Y
Sbjct: 133 LKDNPSETIIMSLKKEY 149
>sp|P45723|PLC_STAAU 1-phosphatidylinositol phosphodiesterase precursor
(Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol-specific phospholipase C) (PI-PLC)
Length = 311
Score = 45.1 bits (105), Expect = 3e-04
Identities = 47/182 (25%), Positives = 71/182 (39%), Gaps = 11/182 (6%)
Frame = +3
Query: 90 NPSDWMSNLPETLTKLPLNWIAIPGTHDSFTYKINSHSHVSPDAEDIVIKLISIFGSWAV 269
+P +WMS L + +N IPG+HDS ++ +
Sbjct: 17 SPENWMSKLDDGKHLTEIN---IPGSHDSGSFTLKD------------------------ 49
Query: 270 SVISRWAKAQDLDPYGQLNAGIRYFDIR---SAIN--DRDGNEIYSCHSLFSGNIRDDLC 434
V S WAK QD D Q+ +G+R+FDIR SA N +Y H L G DD
Sbjct: 50 PVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMISVHHGMVYLHHEL--GKFLDD-- 105
Query: 435 AINNFLKTHQKEVVLLDLNHFYNF------TQHDHMRLLHYLETIFSDKLVPYSRTIPTL 596
+L + E +++ + Y+ T + R +Y + + S PTL
Sbjct: 106 -AKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYYYNNPQYQNLFYTGSNANPTL 164
Query: 597 SE 602
E
Sbjct: 165 KE 166
>sp|O07664|HPRK_ENTFA HPr kinase/phosphorylase (HPrK/P) (HPr(Ser) kinase/phosphorylase)
Length = 311
Score = 32.0 bits (71), Expect = 2.2
Identities = 13/51 (25%), Positives = 29/51 (56%)
Frame = +3
Query: 435 AINNFLKTHQKEVVLLDLNHFYNFTQHDHMRLLHYLETIFSDKLVPYSRTI 587
++N +KT + L+L ++N+ HD ++L E F+++++P R +
Sbjct: 25 SLNRTIKTGEISRPGLELTGYFNYYSHDRLQLFGSKEITFAERMMPEERLL 75
>sp|P44207|YE76_HAEIN Putative HTH-type transcriptional regulator HI1476
Length = 239
Score = 30.8 bits (68), Expect = 5.0
Identities = 20/69 (28%), Positives = 32/69 (46%), Gaps = 6/69 (8%)
Frame = +3
Query: 303 LDPYGQLNAGIRYFDIRSAINDRDGNEIYSCHSLFSGNIRDDLCAI-----NNFLKT-HQ 464
+D Y +N + I DG E YS L S ++ D CA+ N+ L T +
Sbjct: 104 IDSYSSINVSAGFGSFNEGITQPDGQEPYSDELLTSLGVKADNCAVFWANGNSMLPTINN 163
Query: 465 KEVVLLDLN 491
+ +L+DL+
Sbjct: 164 YDQMLVDLS 172
>sp|Q03306|PKH3_YEAST Serine/threonine-protein kinase PKH3 (Pkb-activating kinase homolog
3)
Length = 898
Score = 30.8 bits (68), Expect = 5.0
Identities = 14/36 (38%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
Frame = +3
Query: 45 FSRFTRKSYVHLQNYNPSDWMSNLP--ETLTKLPLN 146
F+ TRK ++ L+ Y W +LP TL+K+P N
Sbjct: 601 FNELTRKGFLILEKYKNRIWFISLPSYSTLSKIPFN 636
>sp|P13010|KU86_HUMAN ATP-dependent DNA helicase II, 80 kDa subunit (Lupus Ku autoantigen
protein p86) (Ku86) (Ku80) (86 kDa subunit of Ku
antigen) (Thyroid-lupus autoantigen) (TLAA) (CTC box
binding factor 85 kDa subunit) (CTCBF) (CTC85) (Nuclear
factor IV) (DNA-repair protein XRCC5)
Length = 732
Score = 30.4 bits (67), Expect = 6.5
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +3
Query: 441 NNFLKTHQKEVVLLDLNHFYNFTQHDHMRLL 533
NNFLK Q++V + LNHF+ D + L+
Sbjct: 656 NNFLKALQEKVEIKQLNHFWEIVVQDGITLI 686
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 107,537,714
Number of Sequences: 369166
Number of extensions: 2342080
Number of successful extensions: 5280
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5275
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8341863645
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)