Planaria EST Database


DrC_00447

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00447
         (1021 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O17453|PSD4_SCHMA  26S proteasome non-ATPase regulatory s...   256   6e-68
sp|P55036|PSD4_HUMAN  26S proteasome non-ATPase regulatory s...   238   2e-62
sp|O35226|PSD4_MOUSE  26S proteasome non-ATPase regulatory s...   236   6e-62
sp|P55035|PSD4_DROME  26S proteasome non-ATPase regulatory s...   209   1e-53
sp|P55034|PSD4_ARATH  26S proteasome non-ATPase regulatory s...   158   2e-38
sp|P38886|RPN10_YEAST  26S proteasome regulatory subunit RPN10    142   2e-33
sp|O94444|RPN10_SCHPO  26S proteasome regulatory subunit rpn10    120   8e-27
sp|Q6GDD5|ICAC_STAAR  Biofilm PIA synthesis protein icaC (In...    31   5.0  
>sp|O17453|PSD4_SCHMA 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
           regulatory subunit S5A)
          Length = 420

 Score =  256 bits (655), Expect = 6e-68
 Identities = 142/323 (43%), Positives = 197/323 (60%), Gaps = 18/323 (5%)
 Frame = +1

Query: 1   DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLANYQVVCTLTTDMSKLHSK 180
           D+MRNGD+ P RLQAQ DA      +K++ N ENT GL++LAN +V+CTLT D+SK++++
Sbjct: 14  DYMRNGDFFPTRLQAQNDAVGLICQSKRQRNPENTIGLLSLANTEVLCTLTNDVSKIYNR 73

Query: 181 ITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXXX 360
           + LV+  G I FC+++R++ LALRHRQ R+QK RIV F+GSPI                 
Sbjct: 74  LHLVEPKGRIIFCSSIRIAHLALRHRQLRHQKMRIVCFIGSPILEDEKELTRLAKRLKKE 133

Query: 361 XVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEES 540
            VN+D+I+FGE+E N+ KLS FID +NGKDG+GSHL+++  G+ L ++L TSP++  E+ 
Sbjct: 134 KVNVDIINFGENETNEQKLSEFIDTLNGKDGTGSHLISVAPGTVLHDTLMTSPVVAGEDG 193

Query: 541 GSLGAVG----FGMEEIDDPDLLYALRVSMEDQRMQQERA----XXXXXXXXXXXXXXXX 696
             +   G    FG++  +DPDLLYALRVSMEDQRM+QE                      
Sbjct: 194 SGMAGAGLGLEFGLDGAEDPDLLYALRVSMEDQRMRQEHEVNGDGSNTSVVATSLPAGSG 253

Query: 697 XSEEDMLREAMAASLSDNIGE-------VDFAAMTEEEQIAYATRMSLQ---DEAGAVGS 846
            SEE ML++A+A S+  N  E       +D AAM+EE+QIAYA RMSLQ   +E     +
Sbjct: 254 TSEEAMLQQALAMSMQMNNTESSSLPMDIDLAAMSEEDQIAYALRMSLQQMGEETTQPTT 313

Query: 847 FGETMDTVVEEPEKEQFDVVDNP 915
                D  + EP     D+   P
Sbjct: 314 TTLESDKTIVEPSGVAMDIDQTP 336
>sp|P55036|PSD4_HUMAN 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
            regulatory subunit S5A) (Rpn10) (Multiubiquitin chain
            binding protein) (Antisecretory factor 1) (AF) (ASF)
          Length = 377

 Score =  238 bits (608), Expect = 2e-62
 Identities = 140/329 (42%), Positives = 192/329 (58%), Gaps = 14/329 (4%)
 Frame = +1

Query: 1    DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLAN-YQVVCTLTTDMSKLHS 177
            ++MRNGD+ P RLQAQ DA N   ++K R N EN  GL+TLAN  +V+ TLT D  ++ S
Sbjct: 14   EYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILS 73

Query: 178  KITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXX 357
            K+  V   G I FCT +RV+ LAL+HRQ +N K RI+ FVGSP+                
Sbjct: 74   KLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKK 133

Query: 358  XXVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEE 537
              VN+D+I+FGE E N  KL+AF++ +NGKDG+GSHLVT+P G SL ++L +SPIL  E 
Sbjct: 134  EKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEG 193

Query: 538  SGSLGA----VGFGMEEIDDPDLLYALRVSMEDQRMQQE----RAXXXXXXXXXXXXXXX 693
               LG       FG++   DP+L  ALRVSME+QR +QE    RA               
Sbjct: 194  GAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGT 253

Query: 694  XXSEEDMLREAMAASLSDNIGEVDFAAMTEEEQIAYATRMSLQD-EAGAVGS----FGET 858
              S++ +L+  ++       G  D ++MTEEEQIAYA +MSLQ  E G   S        
Sbjct: 254  EDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSA 313

Query: 859  MDTVVEEPEKEQFDVVDNPDLIGAVLEGM 945
            MDT     E++ +DV+ +P+ + +VLE +
Sbjct: 314  MDTSEPAKEEDDYDVMQDPEFLQSVLENL 342
>sp|O35226|PSD4_MOUSE 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
           regulatory subunit S5A) (Rpn10) (Multiubiquitin chain
           binding protein)
          Length = 376

 Score =  236 bits (603), Expect = 6e-62
 Identities = 138/328 (42%), Positives = 190/328 (57%), Gaps = 13/328 (3%)
 Frame = +1

Query: 1   DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLAN-YQVVCTLTTDMSKLHS 177
           ++MRNGD+ P RLQAQ DA N   ++K R N EN  GL+TLAN  +V+ TLT D  ++ S
Sbjct: 14  EYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILS 73

Query: 178 KITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXX 357
           K+  V   G I FCT +RV+ LAL+HRQ +N K RI+ FVGSP+                
Sbjct: 74  KLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKK 133

Query: 358 XXVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEE 537
             VN+D+I+FGE E N  KL+AF++ +NGKDG+GSHLVT+P G SL ++L +SPIL  E 
Sbjct: 134 EKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEG 193

Query: 538 SGSLGA----VGFGMEEIDDPDLLYALRVSMEDQRMQQE----RAXXXXXXXXXXXXXXX 693
              LG       FG++   DP+L  ALRVSME+QR +QE    RA               
Sbjct: 194 GAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATPGT 253

Query: 694 XXSEEDMLREAMAASLSDNIGEVDFAAMTEEEQIAYATRMSLQ----DEAGAVGSFGETM 861
             S++ +L+  +        G  D ++MTEEEQIAYA +MSLQ     +  A       M
Sbjct: 254 EDSDDALLKMTINQQEFGRPGLPDLSSMTEEEQIAYAMQMSLQGTEFSQESADMDASSAM 313

Query: 862 DTVVEEPEKEQFDVVDNPDLIGAVLEGM 945
           DT     E++ +DV+ +P+ + +VLE +
Sbjct: 314 DTSDPVKEEDDYDVMQDPEFLQSVLENL 341
>sp|P55035|PSD4_DROME 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
           regulatory subunit S5A) (Multiubiquitin chain binding
           protein) (54 kDa subunit of mu particle) (p54)
          Length = 396

 Score =  209 bits (531), Expect = 1e-53
 Identities = 133/337 (39%), Positives = 182/337 (54%), Gaps = 32/337 (9%)
 Frame = +1

Query: 1   DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLAN-YQVVCTLTTDMSKLHS 177
           DF RNGDY P RL  Q D  N     K R N EN  GL+TL+N  +V+ TLT+D  ++ S
Sbjct: 14  DFQRNGDYFPTRLIVQRDGINLVCLTKLRSNPENNVGLMTLSNTVEVLATLTSDAGRIFS 73

Query: 178 KITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXX 357
           K+ LV   G IN  T +R++ L L+HRQ +N K RIVVFVGSPI                
Sbjct: 74  KMHLVQPKGEINLLTGIRIAHLVLKHRQGKNHKMRIVVFVGSPINHEEGDLVKQAKRLKK 133

Query: 358 XXVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEE 537
             VN+D++SFG+   N   L+AFI+A+NGKDG+GSHLV++P GS L ++L +SPI+  E+
Sbjct: 134 EKVNVDIVSFGDHGNNNEILTAFINALNGKDGTGSHLVSVPRGSVLSDALLSSPIIQGED 193

Query: 538 S-GSLGAVG----FGMEEIDDPDLLYALRVSMEDQRMQQER------------------- 645
             G  G  G    FG++  +DP+L  ALRVSME+QR +QE                    
Sbjct: 194 GMGGAGLGGNVFEFGVDPNEDPELALALRVSMEEQRQRQESEQRRANPDGAPPTGGDAGG 253

Query: 646 ----AXXXXXXXXXXXXXXXXXSEEDMLREAMAASL---SDNIGEVDFAAMTEEEQIAYA 804
               +                 +EE ML+ A+A S     DN+   DFA MTEEEQIA+A
Sbjct: 254 GGGVSGSGPGNEESAGAENEANTEEAMLQRALALSTETPEDNL--PDFANMTEEEQIAFA 311

Query: 805 TRMSLQDEAGAVGSFGETMDTVVEEPEKEQFDVVDNP 915
            +MS+QD            D+V ++ ++ + D  + P
Sbjct: 312 MQMSMQDAPD---------DSVTQQAKRPKTDEANAP 339
>sp|P55034|PSD4_ARATH 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
           regulatory subunit S5A) (Multiubiquitin chain binding
           protein)
          Length = 386

 Score =  158 bits (400), Expect = 2e-38
 Identities = 102/310 (32%), Positives = 168/310 (54%), Gaps = 34/310 (10%)
 Frame = +1

Query: 1   DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLAN--YQVVCTLTTDMSKLH 174
           ++MRNGDY+P+RLQAQ +A N    AK + N ENT G++T+A    +V+ T T+D+ K+ 
Sbjct: 14  EWMRNGDYSPSRLQAQTEAVNLLCGAKTQSNPENTVGILTMAGKGVRVLTTPTSDLGKIL 73

Query: 175 SKITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXX 354
           + +  +D+ G IN    ++++QLAL+HRQ++NQ+ RI+VF GSPI               
Sbjct: 74  ACMHGLDVGGEINLTAAIQIAQLALKHRQNKNQRQRIIVFAGSPIKYEKKALEIVGKRLK 133

Query: 355 XXXVNIDVISFGE--SEENQHKLSAFIDAINGKDGSGSHLVTIPAG-SSLQESLRTSPIL 525
              V++D+++FGE   EE   KL A + A+N  D  GSH+V +P+G ++L + L ++P+ 
Sbjct: 134 KNSVSLDIVNFGEDDDEEKPQKLEALLTAVNNND--GSHIVHVPSGANALSDVLLSTPVF 191

Query: 526 MNEESGS------------LGAVGFGMEEIDDPDLLYALRVSMEDQRMQQERA---XXXX 660
             +E  S             G   FG++   DP+L  ALRVSME++R +QE A       
Sbjct: 192 TGDEGASGYVSAAAAAAAAGGDFDFGVDPNIDPELALALRVSMEEERARQEAAAKKAADE 251

Query: 661 XXXXXXXXXXXXXSEEDMLREA-------------MAASLSDNIGEVDFAAMTEEEQ-IA 798
                        S+E + R               +  +++ ++G+V+ +   +E+Q +A
Sbjct: 252 AGQKDKDGDTASASQETVARTTDKNAEPMDEDSALLDQAIAMSVGDVNMSEAADEDQDLA 311

Query: 799 YATRMSLQDE 828
            A +MS+  E
Sbjct: 312 LALQMSMSGE 321
>sp|P38886|RPN10_YEAST 26S proteasome regulatory subunit RPN10
          Length = 268

 Score =  142 bits (358), Expect = 2e-33
 Identities = 82/232 (35%), Positives = 128/232 (55%), Gaps = 17/232 (7%)
 Frame = +1

Query: 1   DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLA--NYQVVCTLTTDMSKLH 174
           ++ RNGD+   R +AQID+  +   AK+  N ENT GL++ A  N +V+ T T +  K+ 
Sbjct: 14  EYSRNGDFPRTRFEAQIDSVEFIFQAKRNSNPENTVGLISGAGANPRVLSTFTAEFGKIL 73

Query: 175 SKITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXX 354
           + +    + G ++  T ++++QL L+HRQ++ Q  RIV FV SPI               
Sbjct: 74  AGLHDTQIEGKLHMATALQIAQLTLKHRQNKVQHQRIVAFVCSPISDSRDELIRLAKTLK 133

Query: 355 XXXVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTI-PAGSSLQESLRTSPILMN 531
              V +D+I+FGE E+N   L  FI A+N      SHL+T+ P    L E++ +SPI++ 
Sbjct: 134 KNNVAVDIINFGEIEQNTELLDEFIAAVNNPQEETSHLLTVTPGPRLLYENIASSPIILE 193

Query: 532 EESGSLGAVG--------------FGMEEIDDPDLLYALRVSMEDQRMQQER 645
           E S  +GA G              FG++   DP+L  ALR+SME+++ +QER
Sbjct: 194 EGSSGMGAFGGSGGDSDANGTFMDFGVDPSMDPELAMALRLSMEEEQQRQER 245
>sp|O94444|RPN10_SCHPO 26S proteasome regulatory subunit rpn10
          Length = 243

 Score =  120 bits (300), Expect = 8e-27
 Identities = 79/218 (36%), Positives = 112/218 (51%), Gaps = 2/218 (0%)
 Frame = +1

Query: 1   DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLANY--QVVCTLTTDMSKLH 174
           ++M NGDY P R +AQ D  +   N K   N EN CGL+T+ +   QV+ TLT D  K  
Sbjct: 14  EWMINGDYIPTRFEAQKDTVHMIFNQKINDNPENMCGLMTIGDNSPQVLSTLTRDYGKFL 73

Query: 175 SKITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXX 354
           S +  + + GN  F   ++++QLAL+HR+++ Q+ RIV FVGSPI               
Sbjct: 74  SAMHDLPVRGNAKFGDGIQIAQLALKHRENKIQRQRIVAFVGSPIVEDEKNLIRLAKRMK 133

Query: 355 XXXVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNE 534
              V ID+I  GE  +N+  L  FIDA N  D    HLV+IP    L   L     +   
Sbjct: 134 KNNVAIDIIHIGEL-QNESALQHFIDAANSSD--SCHLVSIPPSPQLLSDLVNQSPIGQG 190

Query: 535 ESGSLGAVGFGMEEIDDPDLLYALRVSMEDQRMQQERA 648
              S     +G++   D +L  AL +SM ++R +QE A
Sbjct: 191 VVASQNQFEYGVDPNLDVELALALELSMAEERARQEVA 228
>sp|Q6GDD5|ICAC_STAAR Biofilm PIA synthesis protein icaC (Intercellular adhesion protein C)
          Length = 350

 Score = 31.2 bits (69), Expect = 5.0
 Identities = 18/71 (25%), Positives = 36/71 (50%), Gaps = 7/71 (9%)
 Frame = -2

Query: 1014 LKLYSNNLIVFSTIRFIIIDIMIH-------TFKYCTY*IRIIHDIKLFFFRFFNHSIHC 856
            L+ Y  N+++F T  FII+  ++        T++Y T  ++ I    +    F+++S   
Sbjct: 42   LQFYIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESL 101

Query: 855  LTETTNSSSFV 823
            LT+T+    F+
Sbjct: 102  LTDTSFQKQFI 112
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,138,876
Number of Sequences: 369166
Number of extensions: 1816836
Number of successful extensions: 4900
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4745
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4872
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10909662060
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00447

  1. Dr_sW_011_M01
  2. Dr_sW_003_K08