Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_M01
(783 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O17453|PSD4_SCHMA 26S proteasome non-ATPase regulatory s... 234 2e-61
sp|P55036|PSD4_HUMAN 26S proteasome non-ATPase regulatory s... 204 2e-52
sp|O35226|PSD4_MOUSE 26S proteasome non-ATPase regulatory s... 202 8e-52
sp|P55035|PSD4_DROME 26S proteasome non-ATPase regulatory s... 190 3e-48
sp|P55034|PSD4_ARATH 26S proteasome non-ATPase regulatory s... 158 2e-38
sp|P38886|RPN10_YEAST 26S proteasome regulatory subunit RPN10 142 1e-33
sp|O94444|RPN10_SCHPO 26S proteasome regulatory subunit rpn10 120 5e-27
sp|Q9XSC9|TCO2_BOVIN Transcobalamin II precursor (TCII) (TC... 30 7.4
sp|P05175|VAL1_BGMV AL1 protein (40.2 kDa protein) 30 9.7
>sp|O17453|PSD4_SCHMA 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A)
Length = 420
Score = 234 bits (598), Expect = 2e-61
Identities = 125/276 (45%), Positives = 175/276 (63%), Gaps = 15/276 (5%)
Frame = +1
Query: 1 DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLANYQVVCTLTTDMSKLHSK 180
D+MRNGD+ P RLQAQ DA +K++ N ENT GL++LAN +V+CTLT D+SK++++
Sbjct: 14 DYMRNGDFFPTRLQAQNDAVGLICQSKRQRNPENTIGLLSLANTEVLCTLTNDVSKIYNR 73
Query: 181 ITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXXX 360
+ LV+ G I FC+++R++ LALRHRQ R+QK RIV F+GSPI
Sbjct: 74 LHLVEPKGRIIFCSSIRIAHLALRHRQLRHQKMRIVCFIGSPILEDEKELTRLAKRLKKE 133
Query: 361 XVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEES 540
VN+D+I+FGE+E N+ KLS FID +NGKDG+GSHL+++ G+ L ++L TSP++ E+
Sbjct: 134 KVNVDIINFGENETNEQKLSEFIDTLNGKDGTGSHLISVAPGTVLHDTLMTSPVVAGEDG 193
Query: 541 GSLGAVG----FGMEEIDDPDLLYALRVSMEDQRMQQERA----XXXXXXXXXXXXXXXX 696
+ G FG++ +DPDLLYALRVSMEDQRM+QE
Sbjct: 194 SGMAGAGLGLEFGLDGAEDPDLLYALRVSMEDQRMRQEHEVNGDGSNTSVVATSLPAGSG 253
Query: 697 XSEEDMLREAMAASLSDNIGE-------VDFAAMTE 783
SEE ML++A+A S+ N E +D AAM+E
Sbjct: 254 TSEEAMLQQALAMSMQMNNTESSSLPMDIDLAAMSE 289
>sp|P55036|PSD4_HUMAN 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A) (Rpn10) (Multiubiquitin chain
binding protein) (Antisecretory factor 1) (AF) (ASF)
Length = 377
Score = 204 bits (519), Expect = 2e-52
Identities = 116/270 (42%), Positives = 158/270 (58%), Gaps = 9/270 (3%)
Frame = +1
Query: 1 DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLAN-YQVVCTLTTDMSKLHS 177
++MRNGD+ P RLQAQ DA N ++K R N EN GL+TLAN +V+ TLT D ++ S
Sbjct: 14 EYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILS 73
Query: 178 KITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXX 357
K+ V G I FCT +RV+ LAL+HRQ +N K RI+ FVGSP+
Sbjct: 74 KLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKK 133
Query: 358 XXVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEE 537
VN+D+I+FGE E N KL+AF++ +NGKDG+GSHLVT+P G SL ++L +SPIL E
Sbjct: 134 EKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEG 193
Query: 538 SGSLGA----VGFGMEEIDDPDLLYALRVSMEDQRMQQE----RAXXXXXXXXXXXXXXX 693
LG FG++ DP+L ALRVSME+QR +QE RA
Sbjct: 194 GAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGT 253
Query: 694 XXSEEDMLREAMAASLSDNIGEVDFAAMTE 783
S++ +L+ ++ G D ++MTE
Sbjct: 254 EDSDDALLKMTISQQEFGRTGLPDLSSMTE 283
>sp|O35226|PSD4_MOUSE 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A) (Rpn10) (Multiubiquitin chain
binding protein)
Length = 376
Score = 202 bits (514), Expect = 8e-52
Identities = 116/270 (42%), Positives = 157/270 (58%), Gaps = 9/270 (3%)
Frame = +1
Query: 1 DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLAN-YQVVCTLTTDMSKLHS 177
++MRNGD+ P RLQAQ DA N ++K R N EN GL+TLAN +V+ TLT D ++ S
Sbjct: 14 EYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILS 73
Query: 178 KITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXX 357
K+ V G I FCT +RV+ LAL+HRQ +N K RI+ FVGSP+
Sbjct: 74 KLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKK 133
Query: 358 XXVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEE 537
VN+D+I+FGE E N KL+AF++ +NGKDG+GSHLVT+P G SL ++L +SPIL E
Sbjct: 134 EKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEG 193
Query: 538 SGSLGA----VGFGMEEIDDPDLLYALRVSMEDQRMQQE----RAXXXXXXXXXXXXXXX 693
LG FG++ DP+L ALRVSME+QR +QE RA
Sbjct: 194 GAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATPGT 253
Query: 694 XXSEEDMLREAMAASLSDNIGEVDFAAMTE 783
S++ +L+ + G D ++MTE
Sbjct: 254 EDSDDALLKMTINQQEFGRPGLPDLSSMTE 283
>sp|P55035|PSD4_DROME 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A) (Multiubiquitin chain binding
protein) (54 kDa subunit of mu particle) (p54)
Length = 396
Score = 190 bits (483), Expect = 3e-48
Identities = 104/220 (47%), Positives = 138/220 (62%), Gaps = 6/220 (2%)
Frame = +1
Query: 1 DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLAN-YQVVCTLTTDMSKLHS 177
DF RNGDY P RL Q D N K R N EN GL+TL+N +V+ TLT+D ++ S
Sbjct: 14 DFQRNGDYFPTRLIVQRDGINLVCLTKLRSNPENNVGLMTLSNTVEVLATLTSDAGRIFS 73
Query: 178 KITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXX 357
K+ LV G IN T +R++ L L+HRQ +N K RIVVFVGSPI
Sbjct: 74 KMHLVQPKGEINLLTGIRIAHLVLKHRQGKNHKMRIVVFVGSPINHEEGDLVKQAKRLKK 133
Query: 358 XXVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEE 537
VN+D++SFG+ N L+AFI+A+NGKDG+GSHLV++P GS L ++L +SPI+ E+
Sbjct: 134 EKVNVDIVSFGDHGNNNEILTAFINALNGKDGTGSHLVSVPRGSVLSDALLSSPIIQGED 193
Query: 538 S-GSLGAVG----FGMEEIDDPDLLYALRVSMEDQRMQQE 642
G G G FG++ +DP+L ALRVSME+QR +QE
Sbjct: 194 GMGGAGLGGNVFEFGVDPNEDPELALALRVSMEEQRQRQE 233
>sp|P55034|PSD4_ARATH 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A) (Multiubiquitin chain binding
protein)
Length = 386
Score = 158 bits (399), Expect = 2e-38
Identities = 90/233 (38%), Positives = 140/233 (60%), Gaps = 17/233 (7%)
Frame = +1
Query: 1 DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLAN--YQVVCTLTTDMSKLH 174
++MRNGDY+P+RLQAQ +A N AK + N ENT G++T+A +V+ T T+D+ K+
Sbjct: 14 EWMRNGDYSPSRLQAQTEAVNLLCGAKTQSNPENTVGILTMAGKGVRVLTTPTSDLGKIL 73
Query: 175 SKITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXX 354
+ + +D+ G IN ++++QLAL+HRQ++NQ+ RI+VF GSPI
Sbjct: 74 ACMHGLDVGGEINLTAAIQIAQLALKHRQNKNQRQRIIVFAGSPIKYEKKALEIVGKRLK 133
Query: 355 XXXVNIDVISFGE--SEENQHKLSAFIDAINGKDGSGSHLVTIPAG-SSLQESLRTSPIL 525
V++D+++FGE EE KL A + A+N D GSH+V +P+G ++L + L ++P+
Sbjct: 134 KNSVSLDIVNFGEDDDEEKPQKLEALLTAVNNND--GSHIVHVPSGANALSDVLLSTPVF 191
Query: 526 MNEESGS------------LGAVGFGMEEIDDPDLLYALRVSMEDQRMQQERA 648
+E S G FG++ DP+L ALRVSME++R +QE A
Sbjct: 192 TGDEGASGYVSAAAAAAAAGGDFDFGVDPNIDPELALALRVSMEEERARQEAA 244
>sp|P38886|RPN10_YEAST 26S proteasome regulatory subunit RPN10
Length = 268
Score = 142 bits (358), Expect = 1e-33
Identities = 82/232 (35%), Positives = 128/232 (55%), Gaps = 17/232 (7%)
Frame = +1
Query: 1 DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLA--NYQVVCTLTTDMSKLH 174
++ RNGD+ R +AQID+ + AK+ N ENT GL++ A N +V+ T T + K+
Sbjct: 14 EYSRNGDFPRTRFEAQIDSVEFIFQAKRNSNPENTVGLISGAGANPRVLSTFTAEFGKIL 73
Query: 175 SKITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXX 354
+ + + G ++ T ++++QL L+HRQ++ Q RIV FV SPI
Sbjct: 74 AGLHDTQIEGKLHMATALQIAQLTLKHRQNKVQHQRIVAFVCSPISDSRDELIRLAKTLK 133
Query: 355 XXXVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTI-PAGSSLQESLRTSPILMN 531
V +D+I+FGE E+N L FI A+N SHL+T+ P L E++ +SPI++
Sbjct: 134 KNNVAVDIINFGEIEQNTELLDEFIAAVNNPQEETSHLLTVTPGPRLLYENIASSPIILE 193
Query: 532 EESGSLGAVG--------------FGMEEIDDPDLLYALRVSMEDQRMQQER 645
E S +GA G FG++ DP+L ALR+SME+++ +QER
Sbjct: 194 EGSSGMGAFGGSGGDSDANGTFMDFGVDPSMDPELAMALRLSMEEEQQRQER 245
>sp|O94444|RPN10_SCHPO 26S proteasome regulatory subunit rpn10
Length = 243
Score = 120 bits (300), Expect = 5e-27
Identities = 79/218 (36%), Positives = 112/218 (51%), Gaps = 2/218 (0%)
Frame = +1
Query: 1 DFMRNGDYNPNRLQAQIDAFNYFTNAKKRMNVENTCGLVTLANY--QVVCTLTTDMSKLH 174
++M NGDY P R +AQ D + N K N EN CGL+T+ + QV+ TLT D K
Sbjct: 14 EWMINGDYIPTRFEAQKDTVHMIFNQKINDNPENMCGLMTIGDNSPQVLSTLTRDYGKFL 73
Query: 175 SKITLVDLSGNINFCTTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXX 354
S + + + GN F ++++QLAL+HR+++ Q+ RIV FVGSPI
Sbjct: 74 SAMHDLPVRGNAKFGDGIQIAQLALKHRENKIQRQRIVAFVGSPIVEDEKNLIRLAKRMK 133
Query: 355 XXXVNIDVISFGESEENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNE 534
V ID+I GE +N+ L FIDA N D HLV+IP L L +
Sbjct: 134 KNNVAIDIIHIGEL-QNESALQHFIDAANSSD--SCHLVSIPPSPQLLSDLVNQSPIGQG 190
Query: 535 ESGSLGAVGFGMEEIDDPDLLYALRVSMEDQRMQQERA 648
S +G++ D +L AL +SM ++R +QE A
Sbjct: 191 VVASQNQFEYGVDPNLDVELALALELSMAEERARQEVA 228
>sp|Q9XSC9|TCO2_BOVIN Transcobalamin II precursor (TCII) (TC II)
Length = 432
Score = 30.0 bits (66), Expect = 7.4
Identities = 16/40 (40%), Positives = 25/40 (62%), Gaps = 3/40 (7%)
Frame = +1
Query: 406 QHKLSAFIDA---INGKDGSGSHLVTIPAGSSLQESLRTS 516
Q K+ FID ++G S H V++PAGSSL++ L+ +
Sbjct: 328 QAKVPKFIDVLLKVSGISPSYRHSVSVPAGSSLEDILKNA 367
>sp|P05175|VAL1_BGMV AL1 protein (40.2 kDa protein)
Length = 353
Score = 29.6 bits (65), Expect = 9.7
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -2
Query: 569 IPKPTAPRLPLSSFIKIGLVLKD 501
+P+P P PLSSF+ I +V++D
Sbjct: 176 VPEPWVPPFPLSSFVNIPVVMQD 198
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.316 0.131 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,980,408
Number of Sequences: 369166
Number of extensions: 1359473
Number of successful extensions: 3148
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 3074
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3127
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7357347200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)