Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_K08
(802 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O17453|PSD4_SCHMA 26S proteasome non-ATPase regulatory s... 178 2e-44
sp|P55036|PSD4_HUMAN 26S proteasome non-ATPase regulatory s... 172 1e-42
sp|O35226|PSD4_MOUSE 26S proteasome non-ATPase regulatory s... 170 5e-42
sp|P55035|PSD4_DROME 26S proteasome non-ATPase regulatory s... 150 5e-36
sp|P55034|PSD4_ARATH 26S proteasome non-ATPase regulatory s... 100 6e-21
sp|P38886|RPN10_YEAST 26S proteasome regulatory subunit RPN10 99 1e-20
sp|O94444|RPN10_SCHPO 26S proteasome regulatory subunit rpn10 74 4e-13
sp|Q6GDD5|ICAC_STAAR Biofilm PIA synthesis protein icaC (In... 31 3.4
sp|Q9XSC9|TCO2_BOVIN Transcobalamin II precursor (TCII) (TC... 30 7.7
sp|P04726|GDA6_WHEAT Alpha/beta-gliadin clone PW1215 precur... 30 7.7
>sp|O17453|PSD4_SCHMA 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A)
Length = 420
Score = 178 bits (451), Expect = 2e-44
Identities = 104/250 (41%), Positives = 143/250 (57%), Gaps = 18/250 (7%)
Frame = +1
Query: 1 TTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXXXXVNIDVISFGESE 180
+++R++ LALRHRQ R+QK RIV F+GSPI VN+D+I+FGE+E
Sbjct: 87 SSIRIAHLALRHRQLRHQKMRIVCFIGSPILEDEKELTRLAKRLKKEKVNVDIINFGENE 146
Query: 181 ENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEESGSLGAVG----FG 348
N+ KLS FID +NGKDG+GSHL+++ G+ L ++L TSP++ E+ + G FG
Sbjct: 147 TNEQKLSEFIDTLNGKDGTGSHLISVAPGTVLHDTLMTSPVVAGEDGSGMAGAGLGLEFG 206
Query: 349 MEEIDDPDLLYALRVSMEDQRMQQERA----XXXXXXXXXXXXXXXXXSEEDMLREAMAA 516
++ +DPDLLYALRVSMEDQRM+QE SEE ML++A+A
Sbjct: 207 LDGAEDPDLLYALRVSMEDQRMRQEHEVNGDGSNTSVVATSLPAGSGTSEEAMLQQALAM 266
Query: 517 SLSDNIGE-------VDFAAMTEEEQIAYATRMSLQ---DEAGAVGSFGETMDTVVEEPE 666
S+ N E +D AAM+EE+QIAYA RMSLQ +E + D + EP
Sbjct: 267 SMQMNNTESSSLPMDIDLAAMSEEDQIAYALRMSLQQMGEETTQPTTTTLESDKTIVEPS 326
Query: 667 KEQFDVVDNP 696
D+ P
Sbjct: 327 GVAMDIDQTP 336
>sp|P55036|PSD4_HUMAN 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A) (Rpn10) (Multiubiquitin chain
binding protein) (Antisecretory factor 1) (AF) (ASF)
Length = 377
Score = 172 bits (435), Expect = 1e-42
Identities = 103/255 (40%), Positives = 144/255 (56%), Gaps = 13/255 (5%)
Frame = +1
Query: 1 TTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXXXXVNIDVISFGESE 180
T +RV+ LAL+HRQ +N K RI+ FVGSP+ VN+D+I+FGE E
Sbjct: 88 TGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEE 147
Query: 181 ENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEESGSLGA----VGFG 348
N KL+AF++ +NGKDG+GSHLVT+P G SL ++L +SPIL E LG FG
Sbjct: 148 VNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFG 207
Query: 349 MEEIDDPDLLYALRVSMEDQRMQQE----RAXXXXXXXXXXXXXXXXXSEEDMLREAMAA 516
++ DP+L ALRVSME+QR +QE RA S++ +L+ ++
Sbjct: 208 VDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQ 267
Query: 517 SLSDNIGEVDFAAMTEEEQIAYATRMSLQD-EAGAVGS----FGETMDTVVEEPEKEQFD 681
G D ++MTEEEQIAYA +MSLQ E G S MDT E++ +D
Sbjct: 268 QEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYD 327
Query: 682 VVDNPDLIGAVLEGM 726
V+ +P+ + +VLE +
Sbjct: 328 VMQDPEFLQSVLENL 342
>sp|O35226|PSD4_MOUSE 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A) (Rpn10) (Multiubiquitin chain
binding protein)
Length = 376
Score = 170 bits (430), Expect = 5e-42
Identities = 101/254 (39%), Positives = 142/254 (55%), Gaps = 12/254 (4%)
Frame = +1
Query: 1 TTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXXXXVNIDVISFGESE 180
T +RV+ LAL+HRQ +N K RI+ FVGSP+ VN+D+I+FGE E
Sbjct: 88 TGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEE 147
Query: 181 ENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEESGSLGA----VGFG 348
N KL+AF++ +NGKDG+GSHLVT+P G SL ++L +SPIL E LG FG
Sbjct: 148 VNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFG 207
Query: 349 MEEIDDPDLLYALRVSMEDQRMQQE----RAXXXXXXXXXXXXXXXXXSEEDMLREAMAA 516
++ DP+L ALRVSME+QR +QE RA S++ +L+ +
Sbjct: 208 VDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATPGTEDSDDALLKMTINQ 267
Query: 517 SLSDNIGEVDFAAMTEEEQIAYATRMSLQ----DEAGAVGSFGETMDTVVEEPEKEQFDV 684
G D ++MTEEEQIAYA +MSLQ + A MDT E++ +DV
Sbjct: 268 QEFGRPGLPDLSSMTEEEQIAYAMQMSLQGTEFSQESADMDASSAMDTSDPVKEEDDYDV 327
Query: 685 VDNPDLIGAVLEGM 726
+ +P+ + +VLE +
Sbjct: 328 MQDPEFLQSVLENL 341
>sp|P55035|PSD4_DROME 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A) (Multiubiquitin chain binding
protein) (54 kDa subunit of mu particle) (p54)
Length = 396
Score = 150 bits (378), Expect = 5e-36
Identities = 98/263 (37%), Positives = 139/263 (52%), Gaps = 31/263 (11%)
Frame = +1
Query: 1 TTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXXXXVNIDVISFGESE 180
T +R++ L L+HRQ +N K RIVVFVGSPI VN+D++SFG+
Sbjct: 88 TGIRIAHLVLKHRQGKNHKMRIVVFVGSPINHEEGDLVKQAKRLKKEKVNVDIVSFGDHG 147
Query: 181 ENQHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEES-GSLGAVG----F 345
N L+AFI+A+NGKDG+GSHLV++P GS L ++L +SPI+ E+ G G G F
Sbjct: 148 NNNEILTAFINALNGKDGTGSHLVSVPRGSVLSDALLSSPIIQGEDGMGGAGLGGNVFEF 207
Query: 346 GMEEIDDPDLLYALRVSMEDQRMQQER-----------------------AXXXXXXXXX 456
G++ +DP+L ALRVSME+QR +QE +
Sbjct: 208 GVDPNEDPELALALRVSMEEQRQRQESEQRRANPDGAPPTGGDAGGGGGVSGSGPGNEES 267
Query: 457 XXXXXXXXSEEDMLREAMAASL---SDNIGEVDFAAMTEEEQIAYATRMSLQDEAGAVGS 627
+EE ML+ A+A S DN+ DFA MTEEEQIA+A +MS+QD
Sbjct: 268 AGAENEANTEEAMLQRALALSTETPEDNL--PDFANMTEEEQIAFAMQMSMQDAPD---- 321
Query: 628 FGETMDTVVEEPEKEQFDVVDNP 696
D+V ++ ++ + D + P
Sbjct: 322 -----DSVTQQAKRPKTDEANAP 339
>sp|P55034|PSD4_ARATH 26S proteasome non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A) (Multiubiquitin chain binding
protein)
Length = 386
Score = 100 bits (248), Expect = 6e-21
Identities = 70/233 (30%), Positives = 118/233 (50%), Gaps = 32/233 (13%)
Frame = +1
Query: 7 VRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXXXXVNIDVISFGE--SE 180
++++QLAL+HRQ++NQ+ RI+VF GSPI V++D+++FGE E
Sbjct: 91 IQIAQLALKHRQNKNQRQRIIVFAGSPIKYEKKALEIVGKRLKKNSVSLDIVNFGEDDDE 150
Query: 181 ENQHKLSAFIDAINGKDGSGSHLVTIPAG-SSLQESLRTSPILMNEESGS---------- 327
E KL A + A+N D GSH+V +P+G ++L + L ++P+ +E S
Sbjct: 151 EKPQKLEALLTAVNNND--GSHIVHVPSGANALSDVLLSTPVFTGDEGASGYVSAAAAAA 208
Query: 328 --LGAVGFGMEEIDDPDLLYALRVSMEDQRMQQERA---XXXXXXXXXXXXXXXXXSEED 492
G FG++ DP+L ALRVSME++R +QE A S+E
Sbjct: 209 AAGGDFDFGVDPNIDPELALALRVSMEEERARQEAAAKKAADEAGQKDKDGDTASASQET 268
Query: 493 MLREA-------------MAASLSDNIGEVDFAAMTEEEQ-IAYATRMSLQDE 609
+ R + +++ ++G+V+ + +E+Q +A A +MS+ E
Sbjct: 269 VARTTDKNAEPMDEDSALLDQAIAMSVGDVNMSEAADEDQDLALALQMSMSGE 321
>sp|P38886|RPN10_YEAST 26S proteasome regulatory subunit RPN10
Length = 268
Score = 99.0 bits (245), Expect = 1e-20
Identities = 58/157 (36%), Positives = 86/157 (54%), Gaps = 15/157 (9%)
Frame = +1
Query: 1 TTVRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXXXXVNIDVISFGESE 180
T ++++QL L+HRQ++ Q RIV FV SPI V +D+I+FGE E
Sbjct: 89 TALQIAQLTLKHRQNKVQHQRIVAFVCSPISDSRDELIRLAKTLKKNNVAVDIINFGEIE 148
Query: 181 ENQHKLSAFIDAINGKDGSGSHLVTI-PAGSSLQESLRTSPILMNEESGSLGAVG----- 342
+N L FI A+N SHL+T+ P L E++ +SPI++ E S +GA G
Sbjct: 149 QNTELLDEFIAAVNNPQEETSHLLTVTPGPRLLYENIASSPIILEEGSSGMGAFGGSGGD 208
Query: 343 ---------FGMEEIDDPDLLYALRVSMEDQRMQQER 426
FG++ DP+L ALR+SME+++ +QER
Sbjct: 209 SDANGTFMDFGVDPSMDPELAMALRLSMEEEQQRQER 245
>sp|O94444|RPN10_SCHPO 26S proteasome regulatory subunit rpn10
Length = 243
Score = 74.3 bits (181), Expect = 4e-13
Identities = 49/141 (34%), Positives = 71/141 (50%)
Frame = +1
Query: 7 VRVSQLALRHRQSRNQKPRIVVFVGSPIYXXXXXXXXXXXXXXXXXVNIDVISFGESEEN 186
++++QLAL+HR+++ Q+ RIV FVGSPI V ID+I GE +N
Sbjct: 91 IQIAQLALKHRENKIQRQRIVAFVGSPIVEDEKNLIRLAKRMKKNNVAIDIIHIGEL-QN 149
Query: 187 QHKLSAFIDAINGKDGSGSHLVTIPAGSSLQESLRTSPILMNEESGSLGAVGFGMEEIDD 366
+ L FIDA N D HLV+IP L L + S +G++ D
Sbjct: 150 ESALQHFIDAANSSD--SCHLVSIPPSPQLLSDLVNQSPIGQGVVASQNQFEYGVDPNLD 207
Query: 367 PDLLYALRVSMEDQRMQQERA 429
+L AL +SM ++R +QE A
Sbjct: 208 VELALALELSMAEERARQEVA 228
>sp|Q6GDD5|ICAC_STAAR Biofilm PIA synthesis protein icaC (Intercellular adhesion protein
C)
Length = 350
Score = 31.2 bits (69), Expect = 3.4
Identities = 18/71 (25%), Positives = 36/71 (50%), Gaps = 7/71 (9%)
Frame = -2
Query: 795 LKLYSNNLIVFSTIRFIIIDIMIH-------TFKYCTY*IRIIHDIKLFFFRFFNHSIHC 637
L+ Y N+++F T FII+ ++ T++Y T ++ I + F+++S
Sbjct: 42 LQFYIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESL 101
Query: 636 LTETTNSSSFV 604
LT+T+ F+
Sbjct: 102 LTDTSFQKQFI 112
>sp|Q9XSC9|TCO2_BOVIN Transcobalamin II precursor (TCII) (TC II)
Length = 432
Score = 30.0 bits (66), Expect = 7.7
Identities = 16/40 (40%), Positives = 25/40 (62%), Gaps = 3/40 (7%)
Frame = +1
Query: 187 QHKLSAFIDA---INGKDGSGSHLVTIPAGSSLQESLRTS 297
Q K+ FID ++G S H V++PAGSSL++ L+ +
Sbjct: 328 QAKVPKFIDVLLKVSGISPSYRHSVSVPAGSSLEDILKNA 367
>sp|P04726|GDA6_WHEAT Alpha/beta-gliadin clone PW1215 precursor (Prolamin)
Length = 296
Score = 30.0 bits (66), Expect = 7.7
Identities = 19/53 (35%), Positives = 28/53 (52%)
Frame = -1
Query: 697 QDYPRHQTVLFQVLQPQYPLSHRNYQQLQLRLEETFE*HKQFAPLQSLQQNQL 539
Q YP+ Q Q QPQ P+S + QQ Q + ++ + +Q LQ + Q QL
Sbjct: 103 QPYPQPQP---QYPQPQQPISQQQAQQQQQQQQQQQQQQQQQQILQQILQQQL 152
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,150,435
Number of Sequences: 369166
Number of extensions: 1298933
Number of successful extensions: 3909
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3797
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3887
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7570361805
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)