Planaria EST Database


DrC_00387

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00387
         (1071 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O75954|TSN9_HUMAN  Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)    44   6e-04
sp|Q8BJU2|TSN9_MOUSE  Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)    43   0.002
sp|Q61451|CD53_MOUSE  Leukocyte surface antigen CD53 (Cell s...    35   0.37 
sp|P24485|CD53_RAT  Leukocyte surface antigen CD53 (Cell sur...    33   1.1  
sp|Q9DCK3|TSN4_MOUSE  Tetraspanin-4 (Tspan-4) (Transmembrane...    33   1.4  
sp|O14817|TSN4_HUMAN  Tetraspanin-4 (Tspan-4) (Transmembrane...    32   4.0  
sp|Q9D6K9|LASS5_MOUSE  LAG1 longevity assurance homolog 5 (T...    31   5.3  
sp|O95859|TSN12_HUMAN  Tetraspanin-12 (Tspan-12) (Transmembr...    30   9.0  
sp|Q8N5B7|LASS5_HUMAN  LAG1 longevity assurance homolog 5          30   9.0  
sp|P16893|TRAP_PLAFA  Thrombospondin-related anonymous prote...    30   9.0  
>sp|O75954|TSN9_HUMAN Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)
          Length = 239

 Score = 44.3 bits (103), Expect = 6e-04
 Identities = 28/126 (22%), Positives = 54/126 (42%), Gaps = 9/126 (7%)
 Frame = +3

Query: 93  ILDHGLMLEITSGSIY--------LTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXX 248
           +L  G+ L ++ G+          L+   L+I +G I+  T   G  G++KE++ +    
Sbjct: 27  LLGVGIWLSVSQGNFATFSPSFPSLSAANLVIAIGTIVMVTGFLGCLGAIKENKCLLLSF 86

Query: 249 XXXXXXXXXXXXXXXXXXXXXPSSLNDSTKAEMKRTLINQYARTRL-ITDAWNLTQNRLR 425
                                   +N++ K ++K  L+  +    + + +AWN+ Q  +R
Sbjct: 87  FIVLLVILLAELILLILFFVYMDKVNENAKKDLKEGLLLYHTENNVGLKNAWNIIQAEMR 146

Query: 426 CCGVED 443
           CCGV D
Sbjct: 147 CCGVTD 152
>sp|Q8BJU2|TSN9_MOUSE Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)
          Length = 239

 Score = 42.7 bits (99), Expect = 0.002
 Identities = 28/126 (22%), Positives = 53/126 (42%), Gaps = 9/126 (7%)
 Frame = +3

Query: 93  ILDHGLMLEITSGSIY--------LTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXX 248
           +L  G+ L ++ G+          L+   L+I +G I+  T   G  G++KE++ +    
Sbjct: 27  LLGVGIWLSVSQGNFATFSPSFPSLSAANLVIAIGTIVMVTGFLGCLGAIKENKCLLLSF 86

Query: 249 XXXXXXXXXXXXXXXXXXXXXPSSLNDSTKAEMKRTLINQYARTRL-ITDAWNLTQNRLR 425
                                   +N++ K ++K  L+       + + +AWN+ Q  +R
Sbjct: 87  FIVLLIILLAELILIILFFVYMDKVNENAKQDLKEGLLLYNTENNVGLKNAWNIIQAEMR 146

Query: 426 CCGVED 443
           CCGV D
Sbjct: 147 CCGVTD 152
>sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
          Length = 219

 Score = 35.0 bits (79), Expect = 0.37
 Identities = 25/100 (25%), Positives = 37/100 (37%)
 Frame = +3

Query: 138 YLTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXXXXXXXXXXXXXXXXXXXXXXXPS 317
           +LT   +L+ VG I+      G  GS+KE++ +                           
Sbjct: 49  FLTLGNILVIVGSIIMVVAFLGCMGSIKENKCLLMSFFVLLLIILLAEVTIAILLFVYEQ 108

Query: 318 SLNDSTKAEMKRTLINQYARTRLITDAWNLTQNRLRCCGV 437
            LN +  AE     I  Y        AW+  Q +L+CCGV
Sbjct: 109 KLN-TLVAEGLNDSIQHYHSDNSTMKAWDFIQTQLQCCGV 147
>sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
           (Leukocyte antigen MRC OX-44)
          Length = 219

 Score = 33.5 bits (75), Expect = 1.1
 Identities = 24/100 (24%), Positives = 38/100 (38%)
 Frame = +3

Query: 138 YLTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXXXXXXXXXXXXXXXXXXXXXXXPS 317
           +LT   +L+ VG I+      G  GS+KE++ +                           
Sbjct: 49  FLTLGNVLVIVGSIIMVVAFLGCMGSIKENKCLLMSFFVLLLLILLAEVTLAILLFVYEK 108

Query: 318 SLNDSTKAEMKRTLINQYARTRLITDAWNLTQNRLRCCGV 437
            +N +  AE     I  Y        AW+  Q++L+CCGV
Sbjct: 109 KIN-TLVAEGLNDSIQHYHSDNSTRMAWDFIQSQLQCCGV 147
>sp|Q9DCK3|TSN4_MOUSE Tetraspanin-4 (Tspan-4) (Transmembrane 4 superfamily member 7)
          Length = 238

 Score = 33.1 bits (74), Expect = 1.4
 Identities = 35/170 (20%), Positives = 61/170 (35%), Gaps = 7/170 (4%)
 Frame = +3

Query: 135 IYLTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXXXXXXXXXXXXXXXXXXXXXXXP 314
           + +TGT+++  +GF+ C        G+LKE++ +                          
Sbjct: 57  LIVTGTFVMA-IGFVGCI-------GALKENKCLLLTFFVLLLLVFLLEATIAVLFFAYS 108

Query: 315 SSLNDSTKAEMKRTLINQYARTRL-ITDAWNLTQNRLRCCGVEDGQWDIYINSYWDNITN 491
             ++   + ++K+ L     +  + +T+AW++ Q   RCCGV +        + W  + N
Sbjct: 109 DKIDSYAQQDLKKGLHLYGTQGNVGLTNAWSIIQTDFRCCGVSN-------YTDWFEVYN 161

Query: 492 IDLNNMNLKISRHNPFYQFVPISCCWLLVD------PQTGWPGTVYRNVK 623
                              VP SCC    D      P T W    Y  VK
Sbjct: 162 ATR----------------VPDSCCLEFSDSCGLHEPGTWWKSPCYETVK 195
>sp|O14817|TSN4_HUMAN Tetraspanin-4 (Tspan-4) (Transmembrane 4 superfamily member 7)
           (Novel antigen 2) (NAG-2)
          Length = 238

 Score = 31.6 bits (70), Expect = 4.0
 Identities = 39/192 (20%), Positives = 64/192 (33%), Gaps = 15/192 (7%)
 Frame = +3

Query: 93  ILDHGLMLEITSGSIY--------LTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXX 248
           +L  G+ L  T GS          L+   LLI  G  +      G  G++KE++ +    
Sbjct: 27  VLGVGIWLAATQGSFATLSSSFPSLSAANLLIITGAFVMAIGFVGCLGAIKENKCLLLTF 86

Query: 249 XXXXXXXXXXXXXXXXXXXXXPSSLNDSTKAEMKRTLINQYARTRL-ITDAWNLTQNRLR 425
                                   ++   + ++K+ L     +  + +T+AW++ Q   R
Sbjct: 87  FLLLLLVFLLEATIAILFFAYTDKIDRYAQQDLKKGLHLYGTQGNVGLTNAWSIIQTDFR 146

Query: 426 CCGVEDGQWDIYINSYWDNITNIDLNNMNLKISRHNPFYQFVPISCCWLLVD------PQ 587
           CCGV +        + W  + N                   VP SCC    +      P 
Sbjct: 147 CCGVSN-------YTDWFEVYNATR----------------VPDSCCLEFSESCGLHAPG 183

Query: 588 TGWPGTVYRNVK 623
           T W    Y  VK
Sbjct: 184 TWWKAPCYETVK 195
>sp|Q9D6K9|LASS5_MOUSE LAG1 longevity assurance homolog 5 (Translocating chain-associating
           membrane protein homolog 4) (TRAM homolog 4)
          Length = 414

 Score = 31.2 bits (69), Expect = 5.3
 Identities = 17/67 (25%), Positives = 27/67 (40%), Gaps = 4/67 (5%)
 Frame = +2

Query: 20  TSFCY----FKFYIITFCISTCYCWYK*YTRPWVNARNNKWLNIFNRNLSFDICRFYFVL 187
           T FC     F +Y+  FC    + W    + PW       W N   + LS ++  +Y   
Sbjct: 139 TKFCESMWRFTYYLCIFCYGIRFLW----SMPWFWDTRQCWYNYPYQPLSRELYYYYITQ 194

Query: 188 HRLFWNL 208
              +W+L
Sbjct: 195 LAFYWSL 201
>sp|O95859|TSN12_HUMAN Tetraspanin-12 (Tspan-12) (Transmembrane 4 superfamily member 12)
           (Tetraspan NET-2)
          Length = 305

 Score = 30.4 bits (67), Expect = 9.0
 Identities = 15/41 (36%), Positives = 20/41 (48%)
 Frame = +3

Query: 375 RTRLITDAWNLTQNRLRCCGVEDGQWDIYINSYWDNITNID 497
           R R +T AWN  Q   +CCGV      +Y    W  +T +D
Sbjct: 142 RYRWLTHAWNFFQREFKCCGV------VYFTD-WLEMTEMD 175
>sp|Q8N5B7|LASS5_HUMAN LAG1 longevity assurance homolog 5
          Length = 392

 Score = 30.4 bits (67), Expect = 9.0
 Identities = 18/67 (26%), Positives = 27/67 (40%), Gaps = 4/67 (5%)
 Frame = +2

Query: 20  TSFCY----FKFYIITFCISTCYCWYK*YTRPWVNARNNKWLNIFNRNLSFDICRFYFVL 187
           T FC     F FY+  FC    + W    + PW       W N   + LS  +  +Y + 
Sbjct: 139 TKFCESMWRFTFYLCIFCYGIRFLW----SSPWFWDIRQCWHNYPFQPLSSGLYHYYIME 194

Query: 188 HRLFWNL 208
              +W+L
Sbjct: 195 LAFYWSL 201
>sp|P16893|TRAP_PLAFA Thrombospondin-related anonymous protein precursor
          Length = 559

 Score = 30.4 bits (67), Expect = 9.0
 Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
 Frame = +3

Query: 312 PSSLNDSTKAEMKRTLINQYARTRLITDAWNLTQNR-LRCCGVEDGQWDIYINSYWDNIT 488
           P S+ DS K   K +          I    N+  NR L  C   DG+ ++Y +S W+N+ 
Sbjct: 165 PDSIQDSLKESRKLSDRGVKIAVFGIGQGINVAFNRFLVGCHPSDGKCNLYADSAWENVK 224

Query: 489 NI 494
           N+
Sbjct: 225 NV 226
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 114,273,167
Number of Sequences: 369166
Number of extensions: 2307805
Number of successful extensions: 5745
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5534
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5742
length of database: 68,354,980
effective HSP length: 112
effective length of database: 47,664,660
effective search space used: 11630177040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00387

  1. Dr_sW_010_H16
  2. Dr_sW_022_L23