Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_H16
(821 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O75954|TSN9_HUMAN Tetraspanin-9 (Tspan-9) (Tetraspan NET-5) 44 4e-04
sp|Q8BJU2|TSN9_MOUSE Tetraspanin-9 (Tspan-9) (Tetraspan NET-5) 43 0.001
sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell s... 35 0.25
sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell sur... 33 0.72
sp|Q9DCK3|TSN4_MOUSE Tetraspanin-4 (Tspan-4) (Transmembrane... 33 0.95
sp|O14817|TSN4_HUMAN Tetraspanin-4 (Tspan-4) (Transmembrane... 32 2.8
sp|Q9D6K9|LASS5_MOUSE LAG1 longevity assurance homolog 5 (T... 31 3.6
sp|O95859|TSN12_HUMAN Tetraspanin-12 (Tspan-12) (Transmembr... 30 6.1
sp|Q8N5B7|LASS5_HUMAN LAG1 longevity assurance homolog 5 30 6.1
sp|P16893|TRAP_PLAFA Thrombospondin-related anonymous prote... 30 6.1
>sp|O75954|TSN9_HUMAN Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)
Length = 239
Score = 44.3 bits (103), Expect = 4e-04
Identities = 28/126 (22%), Positives = 54/126 (42%), Gaps = 9/126 (7%)
Frame = +3
Query: 93 ILDHGLMLEITSGSIY--------LTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXX 248
+L G+ L ++ G+ L+ L+I +G I+ T G G++KE++ +
Sbjct: 27 LLGVGIWLSVSQGNFATFSPSFPSLSAANLVIAIGTIVMVTGFLGCLGAIKENKCLLLSF 86
Query: 249 XXXXXXXXXXXXXXXXXXXXXPSSLNDSTKAEMKRTLINQYARTRL-ITDAWNLTQNRLR 425
+N++ K ++K L+ + + + +AWN+ Q +R
Sbjct: 87 FIVLLVILLAELILLILFFVYMDKVNENAKKDLKEGLLLYHTENNVGLKNAWNIIQAEMR 146
Query: 426 CCGVED 443
CCGV D
Sbjct: 147 CCGVTD 152
>sp|Q8BJU2|TSN9_MOUSE Tetraspanin-9 (Tspan-9) (Tetraspan NET-5)
Length = 239
Score = 42.7 bits (99), Expect = 0.001
Identities = 28/126 (22%), Positives = 53/126 (42%), Gaps = 9/126 (7%)
Frame = +3
Query: 93 ILDHGLMLEITSGSIY--------LTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXX 248
+L G+ L ++ G+ L+ L+I +G I+ T G G++KE++ +
Sbjct: 27 LLGVGIWLSVSQGNFATFSPSFPSLSAANLVIAIGTIVMVTGFLGCLGAIKENKCLLLSF 86
Query: 249 XXXXXXXXXXXXXXXXXXXXXPSSLNDSTKAEMKRTLINQYARTRL-ITDAWNLTQNRLR 425
+N++ K ++K L+ + + +AWN+ Q +R
Sbjct: 87 FIVLLIILLAELILIILFFVYMDKVNENAKQDLKEGLLLYNTENNVGLKNAWNIIQAEMR 146
Query: 426 CCGVED 443
CCGV D
Sbjct: 147 CCGVTD 152
>sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
Length = 219
Score = 35.0 bits (79), Expect = 0.25
Identities = 25/100 (25%), Positives = 37/100 (37%)
Frame = +3
Query: 138 YLTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXXXXXXXXXXXXXXXXXXXXXXXPS 317
+LT +L+ VG I+ G GS+KE++ +
Sbjct: 49 FLTLGNILVIVGSIIMVVAFLGCMGSIKENKCLLMSFFVLLLIILLAEVTIAILLFVYEQ 108
Query: 318 SLNDSTKAEMKRTLINQYARTRLITDAWNLTQNRLRCCGV 437
LN + AE I Y AW+ Q +L+CCGV
Sbjct: 109 KLN-TLVAEGLNDSIQHYHSDNSTMKAWDFIQTQLQCCGV 147
>sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
(Leukocyte antigen MRC OX-44)
Length = 219
Score = 33.5 bits (75), Expect = 0.72
Identities = 24/100 (24%), Positives = 38/100 (38%)
Frame = +3
Query: 138 YLTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXXXXXXXXXXXXXXXXXXXXXXXPS 317
+LT +L+ VG I+ G GS+KE++ +
Sbjct: 49 FLTLGNVLVIVGSIIMVVAFLGCMGSIKENKCLLMSFFVLLLLILLAEVTLAILLFVYEK 108
Query: 318 SLNDSTKAEMKRTLINQYARTRLITDAWNLTQNRLRCCGV 437
+N + AE I Y AW+ Q++L+CCGV
Sbjct: 109 KIN-TLVAEGLNDSIQHYHSDNSTRMAWDFIQSQLQCCGV 147
>sp|Q9DCK3|TSN4_MOUSE Tetraspanin-4 (Tspan-4) (Transmembrane 4 superfamily member 7)
Length = 238
Score = 33.1 bits (74), Expect = 0.95
Identities = 35/170 (20%), Positives = 61/170 (35%), Gaps = 7/170 (4%)
Frame = +3
Query: 135 IYLTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXXXXXXXXXXXXXXXXXXXXXXXP 314
+ +TGT+++ +GF+ C G+LKE++ +
Sbjct: 57 LIVTGTFVMA-IGFVGCI-------GALKENKCLLLTFFVLLLLVFLLEATIAVLFFAYS 108
Query: 315 SSLNDSTKAEMKRTLINQYARTRL-ITDAWNLTQNRLRCCGVEDGQWDIYINSYWDNITN 491
++ + ++K+ L + + +T+AW++ Q RCCGV + + W + N
Sbjct: 109 DKIDSYAQQDLKKGLHLYGTQGNVGLTNAWSIIQTDFRCCGVSN-------YTDWFEVYN 161
Query: 492 IDLNNMNLKISRHNPFYQFVPISCCWLLVD------PQTGWPGTVYRNVK 623
VP SCC D P T W Y VK
Sbjct: 162 ATR----------------VPDSCCLEFSDSCGLHEPGTWWKSPCYETVK 195
>sp|O14817|TSN4_HUMAN Tetraspanin-4 (Tspan-4) (Transmembrane 4 superfamily member 7)
(Novel antigen 2) (NAG-2)
Length = 238
Score = 31.6 bits (70), Expect = 2.8
Identities = 39/192 (20%), Positives = 64/192 (33%), Gaps = 15/192 (7%)
Frame = +3
Query: 93 ILDHGLMLEITSGSIY--------LTGTYLLIFVGFILCFTVCFGTYGSLKEDRFMXXXX 248
+L G+ L T GS L+ LLI G + G G++KE++ +
Sbjct: 27 VLGVGIWLAATQGSFATLSSSFPSLSAANLLIITGAFVMAIGFVGCLGAIKENKCLLLTF 86
Query: 249 XXXXXXXXXXXXXXXXXXXXXPSSLNDSTKAEMKRTLINQYARTRL-ITDAWNLTQNRLR 425
++ + ++K+ L + + +T+AW++ Q R
Sbjct: 87 FLLLLLVFLLEATIAILFFAYTDKIDRYAQQDLKKGLHLYGTQGNVGLTNAWSIIQTDFR 146
Query: 426 CCGVEDGQWDIYINSYWDNITNIDLNNMNLKISRHNPFYQFVPISCCWLLVD------PQ 587
CCGV + + W + N VP SCC + P
Sbjct: 147 CCGVSN-------YTDWFEVYNATR----------------VPDSCCLEFSESCGLHAPG 183
Query: 588 TGWPGTVYRNVK 623
T W Y VK
Sbjct: 184 TWWKAPCYETVK 195
>sp|Q9D6K9|LASS5_MOUSE LAG1 longevity assurance homolog 5 (Translocating chain-associating
membrane protein homolog 4) (TRAM homolog 4)
Length = 414
Score = 31.2 bits (69), Expect = 3.6
Identities = 17/67 (25%), Positives = 27/67 (40%), Gaps = 4/67 (5%)
Frame = +2
Query: 20 TSFCY----FKFYIITFCISTCYCWYK*YTRPWVNARNNKWLNIFNRNLSFDICRFYFVL 187
T FC F +Y+ FC + W + PW W N + LS ++ +Y
Sbjct: 139 TKFCESMWRFTYYLCIFCYGIRFLW----SMPWFWDTRQCWYNYPYQPLSRELYYYYITQ 194
Query: 188 HRLFWNL 208
+W+L
Sbjct: 195 LAFYWSL 201
>sp|O95859|TSN12_HUMAN Tetraspanin-12 (Tspan-12) (Transmembrane 4 superfamily member 12)
(Tetraspan NET-2)
Length = 305
Score = 30.4 bits (67), Expect = 6.1
Identities = 15/41 (36%), Positives = 20/41 (48%)
Frame = +3
Query: 375 RTRLITDAWNLTQNRLRCCGVEDGQWDIYINSYWDNITNID 497
R R +T AWN Q +CCGV +Y W +T +D
Sbjct: 142 RYRWLTHAWNFFQREFKCCGV------VYFTD-WLEMTEMD 175
>sp|Q8N5B7|LASS5_HUMAN LAG1 longevity assurance homolog 5
Length = 392
Score = 30.4 bits (67), Expect = 6.1
Identities = 18/67 (26%), Positives = 27/67 (40%), Gaps = 4/67 (5%)
Frame = +2
Query: 20 TSFCY----FKFYIITFCISTCYCWYK*YTRPWVNARNNKWLNIFNRNLSFDICRFYFVL 187
T FC F FY+ FC + W + PW W N + LS + +Y +
Sbjct: 139 TKFCESMWRFTFYLCIFCYGIRFLW----SSPWFWDIRQCWHNYPFQPLSSGLYHYYIME 194
Query: 188 HRLFWNL 208
+W+L
Sbjct: 195 LAFYWSL 201
>sp|P16893|TRAP_PLAFA Thrombospondin-related anonymous protein precursor
Length = 559
Score = 30.4 bits (67), Expect = 6.1
Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
Frame = +3
Query: 312 PSSLNDSTKAEMKRTLINQYARTRLITDAWNLTQNR-LRCCGVEDGQWDIYINSYWDNIT 488
P S+ DS K K + I N+ NR L C DG+ ++Y +S W+N+
Sbjct: 165 PDSIQDSLKESRKLSDRGVKIAVFGIGQGINVAFNRFLVGCHPSDGKCNLYADSAWENVK 224
Query: 489 NI 494
N+
Sbjct: 225 NV 226
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,265,055
Number of Sequences: 369166
Number of extensions: 1832716
Number of successful extensions: 4574
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4425
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4571
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7907893860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)