Planaria EST Database

DrC_00334

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00334
         (779 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8BH95|ECHM_MOUSE  Enoyl-CoA hydratase, mitochondrial pre...   287   3e-77
sp|P14604|ECHM_RAT  Enoyl-CoA hydratase, mitochondrial precu...   286   3e-77
sp|Q58DM8|ECHM_BOVIN  Enoyl-CoA hydratase, mitochondrial pre...   283   3e-76
sp|P30084|ECHM_HUMAN  Enoyl-CoA hydratase, mitochondrial pre...   280   3e-75
sp|Q5R646|ECHM_PONPY  Enoyl-CoA hydratase, mitochondrial pre...   276   6e-74
sp|P34559|ECHM_CAEEL  Probable enoyl-CoA hydratase, mitochon...   257   3e-68
sp|P64017|ECHA8_MYCBO  Probable enoyl-CoA hydratase echA8 >g...   236   5e-62
sp|O07137|ECHA8_MYCLE  Probable enoyl-CoA hydratase echA8         233   6e-61
sp|Q52995|ECHH_RHIME  Probable enoyl-CoA hydratase                228   1e-59
sp|P76082|PAAF_ECOLI  Probable enoyl-CoA hydratase paaF           191   2e-48

>sp|Q8BH95|ECHM_MOUSE Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score =  287 bits (734), Expect = 3e-77
 Identities = 140/235 (59%), Positives = 179/235 (76%)
 Frame = +2

Query: 44  FKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDK 223
           F+ IIT KKG++ S+GLIQ+NRPK+LNALC  L+ E+ +A+  ++ D  +  I+LTG DK
Sbjct: 33  FQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEQDPAVGAIVLTGGDK 92

Query: 224 SFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDII 403
           +FAAGADIKEM  +++ + YS  FL  WD + +  KP++AAVNGYALGGGCELAMMCDII
Sbjct: 93  AFAAGADIKEMQNRTFQDCYSSKFLSHWDHITRVKKPVIAAVNGYALGGGCELAMMCDII 152

Query: 404 YAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKV 583
           YAGE A+FGQPEI LGTIPGAGGTQRL +A+GKS AME+VLTGD I A++A   GLVSK+
Sbjct: 153 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 212

Query: 584 FPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
           FP E L+EE I+   +I+  S + +  AKE+V A+F  TL +G   EK LFYSTF
Sbjct: 213 FPVEKLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGNKLEKRLFYSTF 267

>sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score =  286 bits (733), Expect = 3e-77
 Identities = 141/244 (57%), Positives = 182/244 (74%)
 Frame = +2

Query: 17  FHKQYYSTGFKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIK 196
           F +      F+ IIT KKG++ S+GLIQ+NRPK+LNALC  L+ E+ +A+  ++ D  + 
Sbjct: 24  FRRFASGANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVG 83

Query: 197 CIILTGSDKSFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGC 376
            I+LTG +K+FAAGADIKEM  +++ + YS  FL  WD + +  KP++AAVNGYALGGGC
Sbjct: 84  AIVLTGGEKAFAAGADIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGC 143

Query: 377 ELAMMCDIIYAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEA 556
           ELAMMCDIIYAGE A+FGQPEI LGTIPGAGGTQRL +A+GKS AME+VLTGD I A++A
Sbjct: 144 ELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDA 203

Query: 557 LAHGLVSKVFPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLF 736
              GLVSK+FP ETL+EE I+   +I+  S + +  AKE+V A+F  TL +G   EK LF
Sbjct: 204 KQAGLVSKIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLF 263

Query: 737 YSTF 748
           YSTF
Sbjct: 264 YSTF 267

>sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score =  283 bits (725), Expect = 3e-76
 Identities = 139/238 (58%), Positives = 179/238 (75%)
 Frame = +2

Query: 35  STGFKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTG 214
           S  F+ IIT+KKGR+ ++GLIQ+NRPK+LNALC  L+ E+ +A+  ++ D  +  I+LTG
Sbjct: 30  SAAFEYIITAKKGRNSNVGLIQLNRPKALNALCNGLIVELNQALQAFEEDPAVGAIVLTG 89

Query: 215 SDKSFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMC 394
            +K FAAGADIKEM   ++   YS  FL  WD + +  KP++AAVNGYALGGGCELAMMC
Sbjct: 90  GEKVFAAGADIKEMQSLTFQNCYSGGFLSHWDQLTRVKKPVIAAVNGYALGGGCELAMMC 149

Query: 395 DIIYAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLV 574
           DIIYAGE A+FGQPEI +GTIPGAGGTQRL +A+GKS AME+VLTGD I A++A   GLV
Sbjct: 150 DIIYAGEKAQFGQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLV 209

Query: 575 SKVFPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
           SK+FP ET++EE I+   +I+  S +    AKE+V A+F  TL +G+  EK LFYSTF
Sbjct: 210 SKIFPVETVVEEAIQCAEKIASNSKIVTAMAKESVNAAFEMTLAEGVKLEKKLFYSTF 267

>sp|P30084|ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score =  280 bits (716), Expect = 3e-75
 Identities = 137/235 (58%), Positives = 177/235 (75%)
 Frame = +2

Query: 44  FKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDK 223
           F+ II  K+G+++++GLIQ+NRPK+LNALC  L+ E+ +A+  ++ D  +  I+LTG DK
Sbjct: 33  FEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKTFEEDPAVGAIVLTGGDK 92

Query: 224 SFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDII 403
           +FAAGADIKEM   S+ + YS  FLK WD + Q  KP++AAVNGYA GGGCELAMMCDII
Sbjct: 93  AFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDII 152

Query: 404 YAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKV 583
           YAGE A+F QPEI +GTIPGAGGTQRL +A+GKS AME+VLTGD I A++A   GLVSK+
Sbjct: 153 YAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 212

Query: 584 FPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
            P ETL+EE I+   +I+  S + +  AKE+V A+F  TL +G   EK LFYSTF
Sbjct: 213 CPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTF 267

>sp|Q5R646|ECHM_PONPY Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score =  276 bits (705), Expect = 6e-74
 Identities = 136/235 (57%), Positives = 175/235 (74%)
 Frame = +2

Query: 44  FKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDK 223
           F+ II  K+G+++++GLIQ+NRPK+LNALC  L+ E+ +A+  ++ D  +  I+LTG DK
Sbjct: 33  FEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDK 92

Query: 224 SFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDII 403
           +FAAGADIKEM   S+ + YS  FLK WD + Q  KP++AAVNGYA GGGCELAMMCDII
Sbjct: 93  AFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQIKKPVIAAVNGYAFGGGCELAMMCDII 152

Query: 404 YAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKV 583
           YAGE A+F QPEI +GTIPGAGGTQRL + +GKS AME+VLTGD I A++A   GLVSK+
Sbjct: 153 YAGEKAQFAQPEILIGTIPGAGGTQRLTRTVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 212

Query: 584 FPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
            P ETL+EE I+   +I+  S +    AKE+V A+F  TL +G   EK LFYSTF
Sbjct: 213 CPVETLVEEAIQCAEKIASNSKIIAAMAKESVNAAFEMTLAEGSKLEKKLFYSTF 267

>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
          Length = 288

 Score =  257 bits (656), Expect = 3e-68
 Identities = 122/228 (53%), Positives = 169/228 (74%)
 Frame = +2

Query: 65  KKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDKSFAAGAD 244
           K G   ++ LI++NRPK+LNALC +LMTE+ +A+   D DK++  I++TGS+++FAAGAD
Sbjct: 38  KVGEKQNVALIKLNRPKALNALCAQLMTELADALEVLDTDKSVGAIVITGSERAFAAGAD 97

Query: 245 IKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDIIYAGENAK 424
           IKEM    +   +S +FL  W  V+   KP++AAVNG+ALGGG ELAMMCDIIYAGE A+
Sbjct: 98  IKEMTNNEFATTFSGSFLSNWTAVSDVKKPVIAAVNGFALGGGNELAMMCDIIYAGEKAR 157

Query: 425 FGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKVFPTETLL 604
           FGQPEI +GTIPGAGGTQR  +A GKS AME+ LTG+ + A+EA  HG+VSK+FP + ++
Sbjct: 158 FGQPEINIGTIPGAGGTQRWARAAGKSFAMEVCLTGNHVTAQEAKEHGIVSKIFPADQVV 217

Query: 605 EETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
            E +K+  +I+  S L ++ AKEAV  ++  TL +GL  E+ LF++TF
Sbjct: 218 GEAVKLGEKIADQSPLIVQMAKEAVNKAYELTLQEGLHFERRLFHATF 265

>sp|P64017|ECHA8_MYCBO Probable enoyl-CoA hydratase echA8
 sp|P64016|ECHA8_MYCTU Probable enoyl-CoA hydratase echA8
          Length = 257

 Score =  236 bits (602), Expect = 5e-62
 Identities = 116/225 (51%), Positives = 159/225 (70%)
 Frame = +2

Query: 74  RDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDKSFAAGADIKE 253
           RD  +G+I +NRP++LNAL  ++M E+  A  E D+D +I  II+TGS K+FAAGADIKE
Sbjct: 10  RDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAGADIKE 69

Query: 254 MVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDIIYAGENAKFGQ 433
           M   ++ + ++ +F   W  +A    P +AAV GYALGGGCELAMMCD++ A + AKFGQ
Sbjct: 70  MADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQ 129

Query: 434 PEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKVFPTETLLEET 613
           PEIKLG +PG GG+QRL +A+GK++AM+L+LTG  +DA EA   GLVS+V P + LL E 
Sbjct: 130 PEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADDLLTEA 189

Query: 614 IKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
                 IS+MSA   + AKEAV  +F ++L +GL+ E+ LF+S F
Sbjct: 190 RATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF 234

>sp|O07137|ECHA8_MYCLE Probable enoyl-CoA hydratase echA8
          Length = 257

 Score =  233 bits (593), Expect = 6e-61
 Identities = 116/221 (52%), Positives = 156/221 (70%)
 Frame = +2

Query: 86  IGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDKSFAAGADIKEMVGK 265
           +G+I +NRP++LNAL  ++M EI  A  E D D ++  I++TGS K FAAGADIKEM   
Sbjct: 14  VGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITGSPKVFAAGADIKEMASL 73

Query: 266 SYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDIIYAGENAKFGQPEIK 445
           ++T+ +  +F   W  +A    P++AAV GYALGGGCELAMMCD++ A + AKFGQPEIK
Sbjct: 74  TFTDAFDADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADTAKFGQPEIK 133

Query: 446 LGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKVFPTETLLEETIKMC 625
           LG +PG GG+QRL +A+GK++AM+L+LTG  IDA EA   GLVS+V   + LL E   + 
Sbjct: 134 LGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRVVLADDLLPEAKAVA 193

Query: 626 NRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
             IS+MS    + AKEAV  SF +TL +GL+ E+ LF+STF
Sbjct: 194 TTISQMSRSATRMAKEAVNRSFESTLAEGLLHERRLFHSTF 234

>sp|Q52995|ECHH_RHIME Probable enoyl-CoA hydratase
          Length = 257

 Score =  228 bits (582), Expect = 1e-59
 Identities = 119/235 (50%), Positives = 160/235 (68%)
 Frame = +2

Query: 44  FKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDK 223
           +++++   +GR   +GLI +NRP++LNAL   LM E+  A+  +D D+ +  I+L GS+K
Sbjct: 3   YETLLVETQGR---VGLITLNRPQALNALNAVLMRELDAALKAFDADRAVGAIVLAGSEK 59

Query: 224 SFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDII 403
           +FAAGADIKEM G  + + Y  +FL  W+ VA   KP++AAV+G+ALGGGCELAMMCD I
Sbjct: 60  AFAAGADIKEMQGLDFVDGYLADFLGGWEHVANARKPMIAAVSGFALGGGCELAMMCDFI 119

Query: 404 YAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKV 583
            A E AKFGQPEI LG IPG GG+QRL +A+GK++AM+L+LTG  +DA EA   GLVS+V
Sbjct: 120 IASETAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLILTGRMMDAAEAERSGLVSRV 179

Query: 584 FPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
              + LLEE +    +I+  S      AKEAV  S   TL +GL  E+ LF S F
Sbjct: 180 VAPDRLLEEALGAAEKIASFSLPAAMMAKEAVNRSLELTLAEGLRFERRLFQSLF 234

>sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF
          Length = 255

 Score =  191 bits (485), Expect = 2e-48
 Identities = 96/221 (43%), Positives = 145/221 (65%)
 Frame = +2

Query: 74  RDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDKSFAAGADIKE 253
           R   + L+ +NRP + NAL   L+ ++   +     D +I   ++TG+ + FAAGAD+ E
Sbjct: 8   RQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFAAGADLNE 67

Query: 254 MVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDIIYAGENAKFGQ 433
           M  K      +    + W  +    KPL+AAVNGYALG GCELA++CD++ AGENA+FG 
Sbjct: 68  MAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGL 127

Query: 434 PEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKVFPTETLLEET 613
           PEI LG +PGAGGTQRL++++GKS A ++VL+G++I A++A   GLVS VFP++  LE  
Sbjct: 128 PEITLGIMPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVFPSDLTLEYA 187

Query: 614 IKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLF 736
           +++ +++++ S L L+AAK+A+  S    L  GL  E+ LF
Sbjct: 188 LQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLF 228

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75,083,965
Number of Sequences: 369166
Number of extensions: 1412628
Number of successful extensions: 3908
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3849
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7308943600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00334

  1. Dr_sW_022_M14
  2. Dr_sW_008_N16