Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_N16
(779 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8BH95|ECHM_MOUSE Enoyl-CoA hydratase, mitochondrial pre... 287 3e-77
sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precu... 286 3e-77
sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial pre... 283 3e-76
sp|P30084|ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial pre... 280 3e-75
sp|Q5R646|ECHM_PONPY Enoyl-CoA hydratase, mitochondrial pre... 276 6e-74
sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochon... 257 3e-68
sp|P64017|ECHA8_MYCBO Probable enoyl-CoA hydratase echA8 >g... 236 5e-62
sp|O07137|ECHA8_MYCLE Probable enoyl-CoA hydratase echA8 233 6e-61
sp|Q52995|ECHH_RHIME Probable enoyl-CoA hydratase 228 1e-59
sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF 191 2e-48
>sp|Q8BH95|ECHM_MOUSE Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 287 bits (734), Expect = 3e-77
Identities = 140/235 (59%), Positives = 179/235 (76%)
Frame = +2
Query: 44 FKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDK 223
F+ IIT KKG++ S+GLIQ+NRPK+LNALC L+ E+ +A+ ++ D + I+LTG DK
Sbjct: 33 FQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEQDPAVGAIVLTGGDK 92
Query: 224 SFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDII 403
+FAAGADIKEM +++ + YS FL WD + + KP++AAVNGYALGGGCELAMMCDII
Sbjct: 93 AFAAGADIKEMQNRTFQDCYSSKFLSHWDHITRVKKPVIAAVNGYALGGGCELAMMCDII 152
Query: 404 YAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKV 583
YAGE A+FGQPEI LGTIPGAGGTQRL +A+GKS AME+VLTGD I A++A GLVSK+
Sbjct: 153 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 212
Query: 584 FPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
FP E L+EE I+ +I+ S + + AKE+V A+F TL +G EK LFYSTF
Sbjct: 213 FPVEKLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGNKLEKRLFYSTF 267
>sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 286 bits (733), Expect = 3e-77
Identities = 141/244 (57%), Positives = 182/244 (74%)
Frame = +2
Query: 17 FHKQYYSTGFKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIK 196
F + F+ IIT KKG++ S+GLIQ+NRPK+LNALC L+ E+ +A+ ++ D +
Sbjct: 24 FRRFASGANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVG 83
Query: 197 CIILTGSDKSFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGC 376
I+LTG +K+FAAGADIKEM +++ + YS FL WD + + KP++AAVNGYALGGGC
Sbjct: 84 AIVLTGGEKAFAAGADIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGC 143
Query: 377 ELAMMCDIIYAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEA 556
ELAMMCDIIYAGE A+FGQPEI LGTIPGAGGTQRL +A+GKS AME+VLTGD I A++A
Sbjct: 144 ELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDA 203
Query: 557 LAHGLVSKVFPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLF 736
GLVSK+FP ETL+EE I+ +I+ S + + AKE+V A+F TL +G EK LF
Sbjct: 204 KQAGLVSKIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLF 263
Query: 737 YSTF 748
YSTF
Sbjct: 264 YSTF 267
>sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 283 bits (725), Expect = 3e-76
Identities = 139/238 (58%), Positives = 179/238 (75%)
Frame = +2
Query: 35 STGFKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTG 214
S F+ IIT+KKGR+ ++GLIQ+NRPK+LNALC L+ E+ +A+ ++ D + I+LTG
Sbjct: 30 SAAFEYIITAKKGRNSNVGLIQLNRPKALNALCNGLIVELNQALQAFEEDPAVGAIVLTG 89
Query: 215 SDKSFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMC 394
+K FAAGADIKEM ++ YS FL WD + + KP++AAVNGYALGGGCELAMMC
Sbjct: 90 GEKVFAAGADIKEMQSLTFQNCYSGGFLSHWDQLTRVKKPVIAAVNGYALGGGCELAMMC 149
Query: 395 DIIYAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLV 574
DIIYAGE A+FGQPEI +GTIPGAGGTQRL +A+GKS AME+VLTGD I A++A GLV
Sbjct: 150 DIIYAGEKAQFGQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLV 209
Query: 575 SKVFPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
SK+FP ET++EE I+ +I+ S + AKE+V A+F TL +G+ EK LFYSTF
Sbjct: 210 SKIFPVETVVEEAIQCAEKIASNSKIVTAMAKESVNAAFEMTLAEGVKLEKKLFYSTF 267
>sp|P30084|ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 280 bits (716), Expect = 3e-75
Identities = 137/235 (58%), Positives = 177/235 (75%)
Frame = +2
Query: 44 FKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDK 223
F+ II K+G+++++GLIQ+NRPK+LNALC L+ E+ +A+ ++ D + I+LTG DK
Sbjct: 33 FEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKTFEEDPAVGAIVLTGGDK 92
Query: 224 SFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDII 403
+FAAGADIKEM S+ + YS FLK WD + Q KP++AAVNGYA GGGCELAMMCDII
Sbjct: 93 AFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDII 152
Query: 404 YAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKV 583
YAGE A+F QPEI +GTIPGAGGTQRL +A+GKS AME+VLTGD I A++A GLVSK+
Sbjct: 153 YAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 212
Query: 584 FPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
P ETL+EE I+ +I+ S + + AKE+V A+F TL +G EK LFYSTF
Sbjct: 213 CPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTF 267
>sp|Q5R646|ECHM_PONPY Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 276 bits (705), Expect = 6e-74
Identities = 136/235 (57%), Positives = 175/235 (74%)
Frame = +2
Query: 44 FKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDK 223
F+ II K+G+++++GLIQ+NRPK+LNALC L+ E+ +A+ ++ D + I+LTG DK
Sbjct: 33 FEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDK 92
Query: 224 SFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDII 403
+FAAGADIKEM S+ + YS FLK WD + Q KP++AAVNGYA GGGCELAMMCDII
Sbjct: 93 AFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQIKKPVIAAVNGYAFGGGCELAMMCDII 152
Query: 404 YAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKV 583
YAGE A+F QPEI +GTIPGAGGTQRL + +GKS AME+VLTGD I A++A GLVSK+
Sbjct: 153 YAGEKAQFAQPEILIGTIPGAGGTQRLTRTVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 212
Query: 584 FPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
P ETL+EE I+ +I+ S + AKE+V A+F TL +G EK LFYSTF
Sbjct: 213 CPVETLVEEAIQCAEKIASNSKIIAAMAKESVNAAFEMTLAEGSKLEKKLFYSTF 267
>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
Length = 288
Score = 257 bits (656), Expect = 3e-68
Identities = 122/228 (53%), Positives = 169/228 (74%)
Frame = +2
Query: 65 KKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDKSFAAGAD 244
K G ++ LI++NRPK+LNALC +LMTE+ +A+ D DK++ I++TGS+++FAAGAD
Sbjct: 38 KVGEKQNVALIKLNRPKALNALCAQLMTELADALEVLDTDKSVGAIVITGSERAFAAGAD 97
Query: 245 IKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDIIYAGENAK 424
IKEM + +S +FL W V+ KP++AAVNG+ALGGG ELAMMCDIIYAGE A+
Sbjct: 98 IKEMTNNEFATTFSGSFLSNWTAVSDVKKPVIAAVNGFALGGGNELAMMCDIIYAGEKAR 157
Query: 425 FGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKVFPTETLL 604
FGQPEI +GTIPGAGGTQR +A GKS AME+ LTG+ + A+EA HG+VSK+FP + ++
Sbjct: 158 FGQPEINIGTIPGAGGTQRWARAAGKSFAMEVCLTGNHVTAQEAKEHGIVSKIFPADQVV 217
Query: 605 EETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
E +K+ +I+ S L ++ AKEAV ++ TL +GL E+ LF++TF
Sbjct: 218 GEAVKLGEKIADQSPLIVQMAKEAVNKAYELTLQEGLHFERRLFHATF 265
>sp|P64017|ECHA8_MYCBO Probable enoyl-CoA hydratase echA8
sp|P64016|ECHA8_MYCTU Probable enoyl-CoA hydratase echA8
Length = 257
Score = 236 bits (602), Expect = 5e-62
Identities = 116/225 (51%), Positives = 159/225 (70%)
Frame = +2
Query: 74 RDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDKSFAAGADIKE 253
RD +G+I +NRP++LNAL ++M E+ A E D+D +I II+TGS K+FAAGADIKE
Sbjct: 10 RDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAGADIKE 69
Query: 254 MVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDIIYAGENAKFGQ 433
M ++ + ++ +F W +A P +AAV GYALGGGCELAMMCD++ A + AKFGQ
Sbjct: 70 MADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQ 129
Query: 434 PEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKVFPTETLLEET 613
PEIKLG +PG GG+QRL +A+GK++AM+L+LTG +DA EA GLVS+V P + LL E
Sbjct: 130 PEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADDLLTEA 189
Query: 614 IKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
IS+MSA + AKEAV +F ++L +GL+ E+ LF+S F
Sbjct: 190 RATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF 234
>sp|O07137|ECHA8_MYCLE Probable enoyl-CoA hydratase echA8
Length = 257
Score = 233 bits (593), Expect = 6e-61
Identities = 116/221 (52%), Positives = 156/221 (70%)
Frame = +2
Query: 86 IGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDKSFAAGADIKEMVGK 265
+G+I +NRP++LNAL ++M EI A E D D ++ I++TGS K FAAGADIKEM
Sbjct: 14 VGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITGSPKVFAAGADIKEMASL 73
Query: 266 SYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDIIYAGENAKFGQPEIK 445
++T+ + +F W +A P++AAV GYALGGGCELAMMCD++ A + AKFGQPEIK
Sbjct: 74 TFTDAFDADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADTAKFGQPEIK 133
Query: 446 LGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKVFPTETLLEETIKMC 625
LG +PG GG+QRL +A+GK++AM+L+LTG IDA EA GLVS+V + LL E +
Sbjct: 134 LGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRVVLADDLLPEAKAVA 193
Query: 626 NRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
IS+MS + AKEAV SF +TL +GL+ E+ LF+STF
Sbjct: 194 TTISQMSRSATRMAKEAVNRSFESTLAEGLLHERRLFHSTF 234
>sp|Q52995|ECHH_RHIME Probable enoyl-CoA hydratase
Length = 257
Score = 228 bits (582), Expect = 1e-59
Identities = 119/235 (50%), Positives = 160/235 (68%)
Frame = +2
Query: 44 FKSIITSKKGRDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDK 223
+++++ +GR +GLI +NRP++LNAL LM E+ A+ +D D+ + I+L GS+K
Sbjct: 3 YETLLVETQGR---VGLITLNRPQALNALNAVLMRELDAALKAFDADRAVGAIVLAGSEK 59
Query: 224 SFAAGADIKEMVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDII 403
+FAAGADIKEM G + + Y +FL W+ VA KP++AAV+G+ALGGGCELAMMCD I
Sbjct: 60 AFAAGADIKEMQGLDFVDGYLADFLGGWEHVANARKPMIAAVSGFALGGGCELAMMCDFI 119
Query: 404 YAGENAKFGQPEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKV 583
A E AKFGQPEI LG IPG GG+QRL +A+GK++AM+L+LTG +DA EA GLVS+V
Sbjct: 120 IASETAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLILTGRMMDAAEAERSGLVSRV 179
Query: 584 FPTETLLEETIKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLFYSTF 748
+ LLEE + +I+ S AKEAV S TL +GL E+ LF S F
Sbjct: 180 VAPDRLLEEALGAAEKIASFSLPAAMMAKEAVNRSLELTLAEGLRFERRLFQSLF 234
>sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF
Length = 255
Score = 191 bits (485), Expect = 2e-48
Identities = 96/221 (43%), Positives = 145/221 (65%)
Frame = +2
Query: 74 RDDSIGLIQMNRPKSLNALCYELMTEIKEAINEYDNDKNIKCIILTGSDKSFAAGADIKE 253
R + L+ +NRP + NAL L+ ++ + D +I ++TG+ + FAAGAD+ E
Sbjct: 8 RQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFAAGADLNE 67
Query: 254 MVGKSYTEVYSKNFLKEWDCVAQTTKPLVAAVNGYALGGGCELAMMCDIIYAGENAKFGQ 433
M K + + W + KPL+AAVNGYALG GCELA++CD++ AGENA+FG
Sbjct: 68 MAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGL 127
Query: 434 PEIKLGTIPGAGGTQRLVKALGKSRAMELVLTGDAIDAKEALAHGLVSKVFPTETLLEET 613
PEI LG +PGAGGTQRL++++GKS A ++VL+G++I A++A GLVS VFP++ LE
Sbjct: 128 PEITLGIMPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVFPSDLTLEYA 187
Query: 614 IKMCNRISKMSALTLKAAKEAVLASFNTTLDQGLITEKTLF 736
+++ +++++ S L L+AAK+A+ S L GL E+ LF
Sbjct: 188 LQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLF 228
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75,083,965
Number of Sequences: 369166
Number of extensions: 1412628
Number of successful extensions: 3908
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3849
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7308943600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)